J6jmcmactyg6ugu1r3bm creative biogene

Creative Biogene

Shirley, New York, US

Creative Biogene provides kits, reagents, and services that help researchers explore questions about gene discovery, regulation, and function. Creative Biogene holds a leadership position in the global market and is committed to improving the human condition through biotechnology.

Creative Biogene is a US-based manufacturer and provider of genomics and proteomics products and services for academic and governmental research institutes, pharmaceutical and biotechnology industry.

Our mission is to become the premier provider of innovative technologies, products, unique tools and services for research discoveries and product development in the areas of biological and biomedical research. Emphasis will be placed on the manufacture and distribution of genomics and proteomics based biological research reagent products of the highest possible quality, which include nucleic acid, protein and antibody based research reagent products and services.

Creative Biogene has not listed any services.

Mutant Library Construction
Price on request

A mutant library is a collection of protein variants which is becoming more and more popular in identification of vital residues controlling physiological activities and the investigation of protein optimization. Creative Biogene which possesses high-throughput gene synthesis and mutagenesis platform and highly experienced staffs... Show more »

A mutant library is a collection of protein variants which is becoming more and more popular in identification of vital residues controlling physiological activities and the investigation of protein optimization. Creative Biogene which possesses high-throughput gene synthesis and mutagenesis platform and highly experienced staffs is able to provide the most affordable mutant library construction services with fastest turnaround time to satisfy your downstream needs.

Creative Biogene is able to provide the following mutant library construction services for your scientific research:

Site-directed Mutagenesis Libraries

By combining de novo gene synthesis and site-directed mutagenesis technique, Creative Biogene can offer the most advanced site-directed mutagenesis library construction services. In these libraries, any given residue can be substituted with any of other 19 common amino acids.

Scanning Point Mutation Libraries

Scanning point mutation is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning that it is able to substitute each amino acid with all 20 amino acids simultaneously. This technique enables to provide a detailed profile of each amino acid at the position.

Randomized and Degenerated Libraries

With the help of advanced degenerate oligonucleotide techniques, Creative Biogene can construct any form of randomization or degenerate of full-length gene in an interested DNA fragment. The mutation frequency can be set to any value between 1 and 12 mutations per kb.

« Show less
Shotgun Library Construction Service
Price on request

A shotgun library is a collection of random-sheared DNA molecules. These libraries typically consist of inserts ranging from 1-10kb and extensively used in DNA sequencing, gene screening and DNA mapping throughout the world. As a leading supplier in shotgun library construction services, Creative Biogene is able to provide you the... Show more »

A shotgun library is a collection of random-sheared DNA molecules. These libraries typically consist of inserts ranging from 1-10kb and extensively used in DNA sequencing, gene screening and DNA mapping throughout the world. As a leading supplier in shotgun library construction services, Creative Biogene is able to provide you the most affordable shotgun library construction services using its sophisticated equipments, advanced technologies and highly experienced staffs to satisfy your downstream needs.

Applications:

Small genome sequencing such as virus, phage, plasmid, etc.;
BAC/Fosmid clone sequencing;
Specific genes’ identification;
Gene screening;
Thorough sequencing with fragment lengths > 9 k and without reference sequence.

« Show less
Fosmid Library Construction
Price on request

Fosmids are similar to cosmids but are based on the bacterial F-plasmid. Fosmids are commonly used for preparing genomic libraries when a uniform insert size is desired. Fosmid library can be applied in whole genome physical map construction, the studies of gene function, gene expression and gene regulation. It is also a useful... Show more »

Fosmids are similar to cosmids but are based on the bacterial F-plasmid. Fosmids are commonly used for preparing genomic libraries when a uniform insert size is desired. Fosmid library can be applied in whole genome physical map construction, the studies of gene function, gene expression and gene regulation. It is also a useful tool in map-based cloning of an important gene and the analysis of gene consistence. As a leading supplier in fosmid library construction services, Creative Biogene is committed to providing you the most affordable fosmid library construction services with fastest turnaround time to satisfy your downstream needs.

Applications:

Construction of whole genome physical maps.
Research of gene function and gene expression regulation.
Map-based cloning of genes conferring important traits.
Gene structure and function analysis.

« Show less
BAC Libraries
Price on request

Custom Libraries Construction Service

Features of Creative Biogene's custom libraries construction service:
Highly experience in extraction target molecules from various samples, such as plant, fungal, bacterial, animal etc.
Customer desired vector and bacteria strain are available
High recombinant rate and low clone... Show more »

Custom Libraries Construction Service

Features of Creative Biogene's custom libraries construction service:
Highly experience in extraction target molecules from various samples, such as plant, fungal, bacterial, animal etc.
Customer desired vector and bacteria strain are available
High recombinant rate and low clone redundancy
Strict quality control
Competitive prices
Fast turnaround time

« Show less
Genomic Library Construction
Price on request

A genomic library is a collection of DNA covering the entire genome of an organism. The genomic library contains all DNA sequences such as expressed genes, non-expressed genes, exons and introns, promoter and terminator regions, intervening DNA sequences etc. It is commonly used for genome mapping, sequencing, particular gene’s... Show more »

A genomic library is a collection of DNA covering the entire genome of an organism. The genomic library contains all DNA sequences such as expressed genes, non-expressed genes, exons and introns, promoter and terminator regions, intervening DNA sequences etc. It is commonly used for genome mapping, sequencing, particular gene’s studying and so on. As a leading supplier in genomic library construction services, Creative Biogene can provide the most affordable genomic library construction services with fastest turnaround time to satisfy your downstream needs.

Applications:

Study the genome sequence of a particular gene.
Determine the complete genome sequence of a given organism.
Genetic engineering.
Study the function of regulatory sequences in vitro.

« Show less
cDNA Library Construction
Price on request

As a pioneer biotechnology company in the world, Creative Biogene can provide high quality cDNA libraries construction service to customers worldwide. Creative Biogene’s advanced technologies and highly experienced staffs are available to provide you a range of cDNA libraries construction service, including standard cDNA library,... Show more »

As a pioneer biotechnology company in the world, Creative Biogene can provide high quality cDNA libraries construction service to customers worldwide. Creative Biogene’s advanced technologies and highly experienced staffs are available to provide you a range of cDNA libraries construction service, including standard cDNA library, subtractive cDNA library, normalized cDNA library, full length cDNA library, yeast two-hybrid cDNA library and SSH cDNA library. Creative Biogene’s goal is to provide you with the most affordable and high-quality cDNA libraries construction service to ensure your satisfaction in a timely and professional manner.

Features of Creative Biogene's cDNA libraries construction service:
Large numbers of primary clones.
Low vector background and high percentage of recombinants.
The cDNAs are cloned directionally into our standard vector or your vector of choice.
Competitive prices.
Fast turnaround time.

« Show less
Genome Editing
Price on request

Advantages of microbial gene editing in Creative Biogene:
A wide range of microorganisms species
Scarless genome editing
Precise editing to every base pair
Fast and high efficiency

There are a lot of applications in microbial gene editing, including but not limited to the following items:
Industrial... Show more »

Advantages of microbial gene editing in Creative Biogene:
A wide range of microorganisms species
Scarless genome editing
Precise editing to every base pair
Fast and high efficiency

There are a lot of applications in microbial gene editing, including but not limited to the following items:
Industrial Applications
Pharmaceutical applications
Environmental applications
Agricultural applications

Creative Biogene offers a large panel of microorganisms’ genome editing services. Our excellent staffs with abundant project experience and professional know-how, will work together with you closely and report the project progress in time. Creative Biogene has confidence in speeding your microbe genome editing project and delivering the final products with fast turnaround time and high-quality.

« Show less
CRISPR Knock-in Cell Lines
Price on request
  • Engineered Cell Lines by CRISPR/Cas9 and TALEN System

Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. We are pleased to announce that Creative Biogene is providing new services for making... Show more »

  • Engineered Cell Lines by CRISPR/Cas9 and TALEN System

Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. We are pleased to announce that Creative Biogene is providing new services for making engineered cell lines.

Creative Biogene can use CRISPR/Cas9 and TALEN System to make various forms of gene modification. We have years of experience in generating the following cell lines.
• Knockout cell lines
• Conditional Knockout cell lines
• Site-specific knock-in cell lines
• Gene Mutation cell lines
• Double gene knockout cell lines
• Gene tagging cell lines

Custom Genome Editing Cell Lines

Our services include:

• Construct generation
Targeting vector construction and confirmation, and select the most efficient engineered nuclease
• Stable cell clones selection
Perform transfection on target cells including HEK293 cells, CHO cells, CHO D44 cells, HeLa cells,
other tumor cells, Mouse ES cells, Human ES cells, Primary cells etc. Select cell by MACS, FACS and screen
gene-edited cell clones. Expand and cryopreserve the engineered cells.
• Gene editing evaluation
Evaluate the target stable cell clones via one or several assays such as Western Blot, ELISA, real-time PCR, or reporter assays etc.

« Show less
Viral Packaging
Price on request

Creative Biogene offers custom viral packaging services which can be tailored to meet your specific requirements.

• Adenovirus Service
• AAV Service
• Lentivirus Service
• Retrovirus Service

Features of Creative Biogene's viral packaging services

Quality: Production of virus in a state-of-the art BSL-2... Show more »

Creative Biogene offers custom viral packaging services which can be tailored to meet your specific requirements.

• Adenovirus Service
• AAV Service
• Lentivirus Service
• Retrovirus Service

Features of Creative Biogene's viral packaging services

Quality: Production of virus in a state-of-the art BSL-2 facility with robust quality control in accordance with NIH Biosafety Level 2 criteria.
High Titer: Using proprietary technologies that are unique and highly efficient, we can easily yield 108 to 109 TU/ml lentivirus/retrovirus without concentrating steps.
Quick Turnaround: Creative Biogene has consolidated its procedure of viral packaging services as a standard production-line resulting in a quick turnaround.
Service Customization: We will do anything in our power to make sure your needs are met. We offer plasmid amplification, custom vector building, and large scale production.

« Show less
RNAi Technology
Price on request

RNAi Service

Creative Biogene has built-up leading scientific, technical and commercial track records, successfully advancing therapeutic programs for numerous major industry and academic partners in a wide range of disease areas. With roots as a pioneer of genome-scale RNAi screening and long-standing contributions towards... Show more »

RNAi Service

Creative Biogene has built-up leading scientific, technical and commercial track records, successfully advancing therapeutic programs for numerous major industry and academic partners in a wide range of disease areas. With roots as a pioneer of genome-scale RNAi screening and long-standing contributions towards refining best practices in this field, Creative Biogene provides leading functional genomics technologies including RNA interference (RNAi) tools for gene silencing (siRNA, shRNA, miRNA) and cutting-edge systems for gene editing and gene knockout. All RNAi research projects carried out by Creative Biogene are custom-designed to address each client's specific experimental, strategic and budgetary guidelines, while always meeting the highest scientific standards in the field. Creative Biogene has developed a unique expertise of handling all steps involved from dsRNA design and production to fully automated screening and data analysis. RNA Interference (RNAi) is a powerful tool used to effectively regulate gene expression and is critical in the drug discovery process. Knockdown of target genes with RNAi reagents can be used to facilitate the identification and validation of drug targets and to elucidate biochemical and cell signaling pathways. RNAi is a specific, potent, and highly successful approach for loss-of-function studies in virtually all eukaryotic organisms. RNAi also offers the promise of a new, faster, more cost-effective, and more versatile alternative to current transgenic mouse knockout technologies.

Creative Biogene utilizes many different RNAi technologies to achieve robust knockdown and help you understand complex biological systems. The professional technical services of Creative Biogene's RNAi service team can help clients achieve the maximal value of RNAi technology and avoid some experimental errors. Creative Biogene's research team will work with you to develop much more extensive and effective solutions. Creative Biogene has been granted multiple patents on DNA-based, shRNA and miRNA platforms that are popularly used as gene silencing tools. Compared to other shRNA-expressing and miRNA-expressing plasmids, Creative Biogene's platform design allows high-level expression of shRNA and miRNA. Viruses that can integrate into the chromosomes of most host cells are often used to carry shRNA or miRNA for RNAi screening because of their high transduction rates.

Creative Biogene offers custom RNAi services for your scientific research as follows:
• siRNA, shRNA and miRNA design and synthesis: customer provides target gene name, sequence or
GeneBank ID and so on.
• Choice of shRNA and miRNA vector.
• siRNA, shRNA and miRNA transfection optimization service.
• Test of inhibitory effect: the mRNA expression is detected by RT- PCR; the protein expression is
detected by Western Blot.
• Provision of experimental reports RNAi sequence, RT-PCR test report, Western Blot picture and so
on.
• Custom preparation of shRNA and miRNA expressing lentiviral particles, determination of the virus
titer and establishment of stable cell lines.

« Show less
Custom Gene Cloning
Price on request

As a leading Gene Synthesis service provider in the world, Creative Biogene has extensive experience in synthesis & cloning of genes/genomic DNA from many species. We can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors. Creative Biogene has... Show more »

As a leading Gene Synthesis service provider in the world, Creative Biogene has extensive experience in synthesis & cloning of genes/genomic DNA from many species. We can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors. Creative Biogene has accomplished hundreds of thousands of gene-synthesis & cloning projects for scientists over the world.

Creative Biogene’s gene synthesis and Cloning service is flexible to accommodate projects from single gene synthesis to whole genome synthesis. Our services include:

•Codon Optimization for any given expression system.
• Synthesis of genes of interest with or without tags, and cloning the target sequence into a standard vector.
•Sub-cloning of your synthetic gene products into a vector of your choice.
•Amplification of specific cDNA by PCR/RT-PCR and cloning into a standard vector or a vector of your choice.

Your Reasons to Choose Creative Biogene:

•All sequences are verified by sequencing and guaranteed error-free.
•Delivery of high quality products and final detailed report.
•Our Custom Services team can help you to save your precious time and speed up your research.

We would like to accommodate your needs and hope that our service is of some help. Please don’t hesitate to contact us

« Show less
MicroRNA (miRNA) Synthesis
Price on request

MicroRNA Agomir/ Antagomir Synthesis Service

Creative Biogene is a global leading agomir/ antagomir service provider for all passionate scientific researchers and our comprehensive synthesis service is supported by Cutting-Edge Chemical Synthesis Technology.

Agomir/antagomir are members of a novel class of chemically... Show more »

MicroRNA Agomir/ Antagomir Synthesis Service

Creative Biogene is a global leading agomir/ antagomir service provider for all passionate scientific researchers and our comprehensive synthesis service is supported by Cutting-Edge Chemical Synthesis Technology.

Agomir/antagomir are members of a novel class of chemically engineered oligonucleotides. Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells. The antisense strand has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 4 cholesterol groups at the 3' end, and full length nucleotide 2'-methoxy modification. Antagomir is single-strand miRNA inhibitor carrying the same modifications functioning by blocking miRNA regulation of target gene expression efficiently.

Creative Biogene provides agomir/ antagomir synthesis service that covers all human, mouse and rat miRNAs in the current miRBase .

Creative Biogene's MicroRNA agomir/ antagomir synthesis service provides unparalleled advantages

¬Our agomir/ antagomir is more resistant to degradation than normal miRNA mimics/inhibitors, making it more stable
and durable.
¬Due to higher cell membrane binding affinity, our agomir/antiagomir exhibits higher transfection efficiency with relatively
less reagent.
¬Creative Biogene's MicroRNA agomir/ antagomir is especially suitable for in vivo use.

Creative Biogene's agomir/ antagomir service provide you top-quality services at most cost effective prices.

We are committed to pave new ways to meet the needs of our valued customers with the best product and service. For any more information, please feel free to contact us! Our customer service representatives are available 24 hours a day, from Monday to Sunday.

« Show less
DNA Synthesis
Price on request

The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers. Creative Biogene provides a full spectrum of high quality specialty Oligonucleotide Synthesis Service that range from oligo synthesis for both research and cGMP grade, oligonucleotide modifications to high... Show more »

The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers. Creative Biogene provides a full spectrum of high quality specialty Oligonucleotide Synthesis Service that range from oligo synthesis for both research and cGMP grade, oligonucleotide modifications to high throughput DNA, RNA oligo synthesis.Our custom DNA oligos are optimized for various synthesis scales, modifications and applications to ensure consistently high quality and fast turnaround time.

You ask for it – we synthesize! Just let us know your demand.

Creative Biogene is able to provide the following services:
DNA Synthesis

• Unmodified DNA Synthesis
• Long Oligonucleotide Synthesis
• Arrays/Libraries Oligonucleotide Synthesis
• DNA modification
• DNA qPCR probes
• Others according to your requirements

« Show less
In vivo Phenotypic Screening
Price on request

Creative Biogene is one of the best partners for scientists in the process of both target-based and phenotypic drug discovery. As an experienced drug discovery company, we utilize a large variety of assays that are designed to support your drug discovery. The goal of our services is to effectively reduce the time and cost of your... Show more »

Creative Biogene is one of the best partners for scientists in the process of both target-based and phenotypic drug discovery. As an experienced drug discovery company, we utilize a large variety of assays that are designed to support your drug discovery. The goal of our services is to effectively reduce the time and cost of your discovery programs by taking advantage of our expertise and innovative technology platforms.

« Show less
CRISPR Knock-out Cell Lines
Price on request
  • Engineered Cell Lines by CRISPR/Cas9 and TALEN System

Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. We are pleased to announce that Creative Biogene is providing new services for making... Show more »

  • Engineered Cell Lines by CRISPR/Cas9 and TALEN System

Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. We are pleased to announce that Creative Biogene is providing new services for making engineered cell lines.

Creative Biogene can use CRISPR/Cas9 and TALEN System to make various forms of gene modification. We have years of experience in generating the following cell lines.
• Knockout cell lines
• Conditional Knockout cell lines
• Site-specific knock-in cell lines
• Gene Mutation cell lines
• Double gene knockout cell lines
• Gene tagging cell lines

Custom Genome Editing Cell Lines

Our services include:

• Construct generation
Targeting vector construction and confirmation, and select the most efficient engineered nuclease
• Stable cell clones selection
Perform transfection on target cells including HEK293 cells, CHO cells, CHO D44 cells, HeLa cells,
other tumor cells, Mouse ES cells, Human ES cells, Primary cells etc. Select cell by MACS, FACS and screen
gene-edited cell clones. Expand and cryopreserve the engineered cells.
• Gene editing evaluation
Evaluate the target stable cell clones via one or several assays such as Western Blot, ELISA, real-time PCR, or reporter assays etc.

« Show less
RNAi Screening
Price on request

Creative Biogene utilizes many different RNAi technologies to achieve robust knockdown and help you understand complex biological systems. The professional technical services of Creative Biogene's RNAi service team can help clients achieve the maximal value of RNAi technology and avoid some experimental errors. Creative... Show more »

Creative Biogene utilizes many different RNAi technologies to achieve robust knockdown and help you understand complex biological systems. The professional technical services of Creative Biogene's RNAi service team can help clients achieve the maximal value of RNAi technology and avoid some experimental errors. Creative Biogene's research team will work with you to develop much more extensive and effective solutions. Creative Biogene has been granted multiple patents on DNA-based, shRNA and miRNA platforms that are popularly used as gene silencing tools. Compared to other shRNA-expressing and miRNA-expressing plasmids, Creative Biogene's platform design allows high-level expression of shRNA and miRNA. Viruses that can integrate into the chromosomes of most host cells are often used to carry shRNA or miRNA for RNAi screening because of their high transduction rates.

Creative Biogene offers custom RNAi services for your scientific research as follows:
• siRNA, shRNA and miRNA design and synthesis: customer provides target gene name, sequence or
GeneBank ID and so on.
• Choice of shRNA and miRNA vector.
• siRNA, shRNA and miRNA transfection optimization service.
• Test of inhibitory effect: the mRNA expression is detected by RT- PCR; the protein expression is
detected by Western Blot.
• Provision of experimental reports RNAi sequence, RT-PCR test report, Western Blot picture and so
on.
• Custom preparation of shRNA and miRNA expressing lentiviral particles, determination of the virus
titer and establishment of stable cell lines.

http://www.creative-biogene.com/Services/RNAi-Service.html

« Show less
Retroviral Production
Price on request

Creative Biogene is pioneering in the development of retroviral technology with years of experience in retroviral services. Creative Biogene's custom retrovirus production is a highly efficient approach to deliver genes of interest for stable expression into a broad range of dividing mammalian cells and it aims to provide... Show more »

Creative Biogene is pioneering in the development of retroviral technology with years of experience in retroviral services. Creative Biogene's custom retrovirus production is a highly efficient approach to deliver genes of interest for stable expression into a broad range of dividing mammalian cells and it aims to provide valuable research tools and services in molecular biology.

Creative Biogene offers custom retrovirus construction services for your scientific research as follows:
• Construction of ordered retrovirus vector (gene expression and shRNA expression).
• Production of a retrovirus producer cell line by transfection.
• Production of viral supernatant by transient transfection as well as assay for replication competent
retrovirus.
• Quality control assays including quantification of genome copies and quantification of retroviral
vector infectivity.

http://www.creative-biogene.com/Services/Custom-Viral-Service/Retrovirus-service.html

« Show less
Lentivirus production
Price on request

Creative Biogene is pioneering in the development of lentiviral technology. With years of experience in research of lentivirus, Creative Biogene provides convenient and efficient services for studies on promoter regulation, target gene over-expression, silencing of genes and so on. Using an innovative platform "CLPS"... Show more »

Creative Biogene is pioneering in the development of lentiviral technology. With years of experience in research of lentivirus, Creative Biogene provides convenient and efficient services for studies on promoter regulation, target gene over-expression, silencing of genes and so on. Using an innovative platform "CLPS" (Creative Lentiviral Packaging System), Creative Biogene generates more lentivirus than other popular systems, has high safety profile and high efficiency of gene delivery to virtually all cell types and whole model organisms, and what is more, it is compatible with any lentiviral vector system.

Creative Biogene offers custom lentivirus construction services for your scientific research as follows:
• Make your lentiviral inducible shRNA vector or lentiviral expression vector for your targets.
• Delivery purified, Ready-To-Use lentivirus for your target of interest at high titer.
• Generate stable cells in the cell line of your interest.
• Select the stably transduced, high expression cells.
• Express protein in suspension mammalian cells using lentiviral transduction.
• Validate the genomic integration of gene via genomic PCR.
• Validate the high-expression clone by Western Blot.
• Purify the mammalian cell expressed proteins.

http://www.creative-biogene.com/Services/Custom-Viral-Service/Lentivirus-service.html

« Show less
AAV Production
Adeno-associated virus (AAV) production
Price on request

Creative Biogene possesses the world-class production facility and manufacture rAAV vectors with the highest possible stringency, resulting in an extremely pure, high quality product every time. Creative Biogene offers the latest generation rAAV stocks optimised for high levels of transgene expression and you can get reliable,... Show more »

Creative Biogene possesses the world-class production facility and manufacture rAAV vectors with the highest possible stringency, resulting in an extremely pure, high quality product every time. Creative Biogene offers the latest generation rAAV stocks optimised for high levels of transgene expression and you can get reliable, reproducible, high purity, high titer vector stocks every time you order. With the latest innovations in rAAV technology, Creative Biogene can apply rAAV virus with the highest standard (>95% purity). When you order the service of Creative Biogene, you are buying top quality products for your research. In recent years, AAV has become increasingly valuable for in vivo studies in animals, and are also currently being tested in human clinical trials. Unlike adenovirus, AAV has its own advantages. Firstly, it is non-pathogenic (most people carry this harmless virus) and does not stimulate inflammation in the host; Also, it elicits little or no immune response; Lastly, AAV viruses can infect non-dividing (quiescent) cells, such as neurons, and remain expression in their genomes for a long time, so clinical trials have been initiated where AAV vectors are used to deliver genes to the brain.

Creative Biogene offers custom AAV construction services for your scientific research as follows:
• Cloning gene of interest into appropriate AAV shuttle plasmid
• Packaging by Transfection of 293T cells with AAV expression vectors and helper plasmids(AAV
serotypes 1- 9).
• Harvest, amplification and rAAV purification
• Desalting, filter sterilization, and titer determination

http://www.creative-biogene.com/Services/Custom-Viral-Service/AAV-service.html

« Show less
Adenovirus production
Price on request

Creative Biogene is a biotechnology company specializing in custom adenovirus production service. Creative Biogene's state-of-the-art facilities and highly experienced staff are available in order to assist in all areas of adenovirus vector design and construction, as well as the generation of the adenovirus in high titer.... Show more »

Creative Biogene is a biotechnology company specializing in custom adenovirus production service. Creative Biogene's state-of-the-art facilities and highly experienced staff are available in order to assist in all areas of adenovirus vector design and construction, as well as the generation of the adenovirus in high titer. Using unique processes for the generation of new adenovirus constructs, Creative Biogene can provide the fastest turn around time of any supplier in the industry.

Recombinant adenoviruses are versatile and highly efficient tools used for gene delivery and expression in mammalian cells. Numerous biological features of adenoviruses have made them the vector of choice for both in vitro and in vivo applications. Adenoviruses can infect a variety of mammalian cell types with high efficiency. They remain epichromosal upon infection, so they are only suitable for transient expression. Creative Biogene offers an array of adenovirus services to meet your needs. Creative Biogene's committed to providing you with the best research materials and support in the adenovirus field.

Creative Biogene offers custom adenovirus construction services for your scientific research as follows:
• Clone gene of interest into appropriate transfer vector.
• Transfect HEK 293 cells with recombinant viral DNA to produce recombinant adenoviruses.
• Create high-titer recombinant adenovirus stock.

http://www.creative-biogene.com/Services/Custom-Viral-Service/Adenovirus-service.html

« Show less
Targeted Bisulfite Sequencing
Price on request

DNA methylation is a key epigenetic modification to cytosines, often in CpG dinucleotides. This modification has been frequently associated with gene silencing, but the precise role of DNA methylation in development and disease remains a mystery.

Genome-wide DNA methylation analysis is now made easily using our comprehensive... Show more »

DNA methylation is a key epigenetic modification to cytosines, often in CpG dinucleotides. This modification has been frequently associated with gene silencing, but the precise role of DNA methylation in development and disease remains a mystery.

Genome-wide DNA methylation analysis is now made easily using our comprehensive DNA methylation analysis services. The study of methylation at single base resolution of individual cytosines in DNA is facilitated by bisulfite treatment of DNA followed by PCR amplification, cloning, and sequencing of individual amplimers.

Creative Biogene offers platform to yield reliable information on the methylation states of individual cytosines by effectively and efficiently preparing converted DNA for use in next generation sequencing techniques. The most reliable and comprehensive data is offered timely to the clients during the experiments. We can help you to speed up your work and we promise to delivery the service you need within shortest turnaround time, so that you can skip the technical hurdles, focus more on the biology your research, and expedite end-results.

« Show less
Cloning Vectors
Price on request

A vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed. Common to all engineered vectors are an origin of replication, a multicloning site, and a selectable marker. The purpose of a vector which transfers genetic information to... Show more »

A vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed. Common to all engineered vectors are an origin of replication, a multicloning site, and a selectable marker. The purpose of a vector which transfers genetic information to another cell is typically to isolate, multiply, or express the insert in the target cell. Vectors called expression vectors (expression constructs) specifically are for the expression of the transgene in the target cell, and generally have a promoter sequence that drives expression of the transgene. Simpler vectors called transcription vectors are only capable of being transcribed but not translated: they can be replicated in a target cell but not expressed, unlike expression vectors. Transcription vectors are used to amplify their insert. Insertion of a vector into the target cell is usually called transformation for bacterial cells, transfection for eukaryotic cells, although insertion of a viral vector is often called transduction.

Creative Biogene offers a wide range of vectors including expression vectors and clone vectors. Choose your vector from Creative Biogene to match your various needs.

« Show less
Cosmid/Fosmids/Phagemid Vector
Price on request

Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids typically lack the genes required for packaging into virions. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as... Show more »

Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids typically lack the genes required for packaging into virions. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification.

Creative Biogene offers phagemid-vectors which can be used in the study of high-throughput screening of protein-protein interactions, selection of proteins with specific binding properties (high affinity binders), enzyme inhibitor screening, development of affinity matrices and ligand screening etc.

« Show less
Oligonucleotide Synthesis
Price on request

The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers. Creative Biogene provides a full spectrum of high quality specialty Oligonucleotide Synthesis Service that range from oligo synthesis for both research and cGMP grade, oligonucleotide modifications to high... Show more »

The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers. Creative Biogene provides a full spectrum of high quality specialty Oligonucleotide Synthesis Service that range from oligo synthesis for both research and cGMP grade, oligonucleotide modifications to high throughput DNA, RNA oligo synthesis.Our custom DNA oligos are optimized for various synthesis scales, modifications and applications to ensure consistently high quality and fast turnaround time.

You ask for it – we synthesize! Just let us know your demand.

Creative Biogene is able to provide the following services:

  • DNA Synthesis
    • Unmodified DNA Synthesis
    • Long Oligonucleotide Synthesis
    • Arrays/Libraries Oligonucleotide Synthesis
    • DNA modification
    • DNA qPCR probes
    • Others according to your requirements
  • RNA Synthesis
    • Unmodified RNA Synthesis
    • Long RNA Oligonucleotide Synthesis
    • RNA Modification
    • Labeled RNA Probes
    • Others according to your requirements
  • Large Scale Oligonucleotide Synthesis
« Show less
Random Mutagenesis
Price on request

Creative Biogene are able to create a random mutagenesis library of any desired complexity to any gene of your interest. This library is made by deliberately introducing errors in the synthesis process. The mutational frequency can be controlled from 0.5 to 5 random amino acid substitutions per 1,000bp gene variant. The randomized... Show more »

Creative Biogene are able to create a random mutagenesis library of any desired complexity to any gene of your interest. This library is made by deliberately introducing errors in the synthesis process. The mutational frequency can be controlled from 0.5 to 5 random amino acid substitutions per 1,000bp gene variant. The randomized portion of the gene can cover the complete open reading frame or desired regions within the peptide.

« Show less
Site-Directed Mutagenesis
Price on request

Creative Biogene uses a targeted PCR-based process to introduce genetic variation precisely where you need it, then clone the construct into the vector of your choice. Creative Biogene's site-directed mutagenesis services include point mutations, deletions, and insertions. They can be customized to fit any scientific applications.

Creative Biogene uses a targeted PCR-based process to introduce genetic variation precisely where you need it, then clone the construct into the vector of your choice. Creative Biogene's site-directed mutagenesis services include point mutations, deletions, and insertions. They can be customized to fit any scientific applications.

« Show less
Gene Synthesis
Price on request

As a leading Gene Synthesis service provider in the world, Creative Biogene has extensive experience in synthesis & cloning of genes/genomic DNA from many species. We can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors. Creative Biogene has... Show more »

As a leading Gene Synthesis service provider in the world, Creative Biogene has extensive experience in synthesis & cloning of genes/genomic DNA from many species. We can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors. Creative Biogene has accomplished hundreds of thousands of gene-synthesis & cloning projects for scientists over the world.

Creative Biogene’s gene synthesis and Cloning service is flexible to accommodate projects from single gene synthesis to whole genome synthesis. Our services include:

  • Codon Optimization for any given expression system.
  • Synthesis of genes of interest with or without tags, and cloning the target sequence into a standard vector.
  • Sub-cloning of your synthetic gene products into a vector of your choice.
  • Amplification of specific cDNA by PCR/RT-PCR and cloning into a standard vector or a vector of your choice.

Your Reasons to Choose Creative Biogene:

  • All sequences are verified by sequencing and guaranteed error-free.
  • Delivery of high quality products and final detailed report.
  • Our Custom Services team can help you to save your precious time and speed up your research.

We would like to accommodate your needs and hope that our service is of some help. Please don’t hesitate to contact us.

« Show less
RNA Synthesis
Price on request

siRNA, shRNA and miRNA design and synthesis: customer provides target gene name, sequence or
GeneBank ID and so on.

siRNA, shRNA and miRNA design and synthesis: customer provides target gene name, sequence or
GeneBank ID and so on.

« Show less
Plasmid DNA Preparation
Price on request

Creative Biogene has been perfecting plasmid DNA production for many years. We offer a wide range of plasmid preparation services for many applications, including research, preclinical, clinical, and diagnostic applications. The plasmid DNA isolation service will be suitable for transfection and animal studies to both... Show more »

Creative Biogene has been perfecting plasmid DNA production for many years. We offer a wide range of plasmid preparation services for many applications, including research, preclinical, clinical, and diagnostic applications. The plasmid DNA isolation service will be suitable for transfection and animal studies to both biotechnology researchers and pharmaceutical companies. Our goal is to provide you with the most affordable, high-quality plasmid DNA manufacturing services that ensure your satisfaction in a timely and professional manner.

Plasmid DNAs serve as important tools in genetics and biotechnology labs where they are commonly used to multiply (make many copies of) or express particular genes. Another major use of plasmids is to make large amounts of proteins from the inserted gene. This is a cheap and easy way of mass-producing a gene or the protein it then codes for, for example, insulin or even antibiotics.

Summary of Our Process:

  • Selection of host strain and growth conditions.
  • High-cell-density fermentation.
  • Cell harvesting, lysis and plasmid purification.
  • Wide range of quality control testing including genomic DNA /RNA /protein contamination test, endotoxin (lipopolysaccharides) assay, restriction profile analysis, DNA Sequencing and OD260/280 readings and so on.

Creative Biogene offers plasmid production services for your scientific research as follows:

  • Construct your plasmid according to your requirements.
  • Produce plasmids for applications both in vitro and in vivo.
  • Robust manufacturing protocols.
  • Flexible scale: up to gram level.
  • High standard quality control.
  • Short turn-around time.
« Show less
Aptamer Synthesis
Price on request

Creative Biogene offers custom aptamer development services for your scientific research as follows:

  • Selection is an in vitro process that can target any small molecule, biopolymer, or cell.
  • Uniform activity regardless of the batch.
  • Aptamers can be rapidly produced in large quantities with easy quality control
  • High... Show more »

Creative Biogene offers custom aptamer development services for your scientific research as follows:

  • Selection is an in vitro process that can target any small molecule, biopolymer, or cell.
  • Uniform activity regardless of the batch.
  • Aptamers can be rapidly produced in large quantities with easy quality control
  • High stability
  • High specificity, aptamers can discriminate enantiomers and protein isoforms
  • Affinity parameters can be controlled on demand
  • Wide variety of chemical modifications are introduced to diversify properties and functions
  • Unlimited shelf-life
  • No evidence of immunogenicity

Aptamers have been attracting more and more attention within life science research and applications owing to these advantages over antibody. Aaptamers are playing their roles in ELISAS, Westerns, Flow Cytometry & Microarrays and are hopeful to replace antibodies in all of the areas currently dominated by antibodies. Moreover, there is one aptamer approved by FDA and several other candidates are currently in clinical trials. Thus, aptamers possess encouraging prospects.

« Show less
Stable Cell Line Generation
Price on request

Creative Biogene offers high quality, fast turnaround and cost-effective Stable Cell Line Generation Service for protein expression, gene knockdown or genome editing that meet your specific research needs. Based on many years' experience on cell line development, Creative Biogene's cell culture scientists focus on... Show more »

Creative Biogene offers high quality, fast turnaround and cost-effective Stable Cell Line Generation Service for protein expression, gene knockdown or genome editing that meet your specific research needs. Based on many years' experience on cell line development, Creative Biogene's cell culture scientists focus on optimizing all parameters specific to your cell lines to efficiently produce stable cell lines according to your requirements. Creative Biogene?provides commercial and research cell line based on its proprietary expression vector, high throughput cell screening technologies and serum, animal components free process without any adaptation issue in a significantly reduced timeline.

Stable cell line is the best solution, not only to produce recombinant protein and antibody, but also for application in drug screenings, gene functional studies etc. Depending on the type of gene expression and the gene construct, there are many different approaches for establishing stable cell lines

« Show less
Animal Model in vivo Analyses
Price on request
Request a quote for more information about this service.
Virus production
Price on request
Request a quote for more information about this service.
Epigenetic Analysis
Price on request
Request a quote for more information about this service.
RNA
Price on request
Request a quote for more information about this service.
Mammalian Cell Culture
Price on request
Request a quote for more information about this service.
Microbiology, Virology, and Parasitology
Price on request
Request a quote for more information about this service.
Molecular Cloning
Price on request
Request a quote for more information about this service.
Animal Models and Studies
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Viral Production and Assays
Price on request
Request a quote for more information about this service.
Aptamers
Price on request
Request a quote for more information about this service.
In vivo Assays
Price on request
Request a quote for more information about this service.
Mutagenesis
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
DNA Libraries
Price on request
Request a quote for more information about this service.
DNA
Price on request
Request a quote for more information about this service.
Animal Physiology Analyses
Price on request
Request a quote for more information about this service.
Cells and Tissues
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
Genetic Engineering
Price on request
Request a quote for more information about this service.
Plasmids and Vectors
Price on request
Request a quote for more information about this service.
Ask A Question Find what you're looking for? If not, you can ask this lab a question:
2017-05-24 11:07:43 -0700

Net Promoter Score of 6 received for Stable Cell Line Generation.

Additional Ratings: satisfaction with deliverable: 6, satisfaction with timeliness: 8.

Be the first to endorse Creative Biogene

Endorse this lab