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Confocal Imaging Facility

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Princeton, New Jersey, US

About Confocal Imaging Facility

Divison of: Princeton University

The confocal microscopy facility provides researchers with the ability to visualize a variety of samples, from monolayers and small organisms, such as developing fly and fish embryos, to very thick sections from brain and other organ tissues.

This is accomplished using a variety of optical imaging modalities, including laser point (LSCM) and field scanning confocal (CSU), Total Internal Reflectance Fluorescence (TIRF), Multi Photon (MP), and Widefield (WF).

These technologies provide us with the highest light microscope resolution obtainable, giving the scientist a clearer picture of macro, cellular, and subcellular structure and function.

We can image single or multiple labeled specimens, using magnifications from 4X to 150X with a temporal resolution up to 400 fps. Our laboratory has experience imaging in mammalian cells, yeast cells, Drosophila and Zebrafish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens.

Quantitative imaging methods such as FRAP, FLIP, and FRET are available. The laboratory also maintains several workstations with software packages for image processing, analysis, and 3D image reconstruction.

Our Services (12)


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Imaging & Spectroscopy

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We can image single or multiple labeled specimens, using magnifications from 4X to 150X with a temporal resolution up to 400 fps. Our laboratory has experience imaging in mammalian cells, yeast cells, Drosophila and Zebrafish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens.


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FRET

Fluorescence resonance energy transfer
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FRAP

Fluorescence recovery after photobleaching
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Widefield Fluorescence Microscopy

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Multiphoton Fluorescence Microscopy

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TIRF

Total Internal Reflection Microscopy
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Laser Scanning Confocal Microscopy

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Spinning Disk Confocal Microscopy

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Fluorescence-Based Microscopy

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Confocal Microscopy

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Microscopy

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Optical Microscopy

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Gary Laevsky

Facility Director

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