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Confocal and Specialized Microscopy Shared Resource

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New York, New York, US

About Confocal and Specialized Microscopy Shared Resource

Divison of: Columbia University

The Confocal and Specialized Microscopy Shared Resource (CSMSR) provides advanced microscope systems for multidimensional optical imaging of living and fixed cells and tissues. The CSMSR offers a wide range of state-of-the-art microscopy technologies, from super-resolution microscopy to laser capture and... Show more »

The Confocal and Specialized Microscopy Shared Resource (CSMSR) provides advanced microscope systems for multidimensional optical imaging of living and fixed cells and tissues. The CSMSR offers a wide range of state-of-the-art microscopy technologies, from super-resolution microscopy to laser capture and microirradiation.

Our full-time staff provide assistance using the microscopes, training for independent use, and technical support. Trained in cell biology and biophysics with in-depth expertise in light microscopy, we can help with experimental design, image acquisition, and data interpretation.

Technologies Offered:
- Confocal microscopy (scanning and spinning-disk)
- Two-photon microscopy
- Wide-field fluorescence microscopy
- Digital deconvolution
- Transmitted-light imaging (phase, DIC, histology)
- Super-resolution imaging (STORM and SIM)
- Cell surface imaging with <100 nM z-resolution (TIRF)
- Laser-capture microdissection
- Microfluidic cell culture while imaging
- Software for image analysis, processing, and presentation

Application Examples:
- Live-cell imaging
- Fluorescence Recovery after Photobleaching (FRAP)
- Förster Resonance Energy Transfer (FRET)
- Colocalization analysis
- Cell and particle tracking studies

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Our Services (23)


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3D Structured Illumination Microscopy

Starting at $134.40 per hour

Nikon N-SIM
2D SIM, TIRF-SIM, 3D SIM
Fluorescence Excitation at 405, 488, 561 nm
Software : NIS-Elements

Nikon N-SIM
2D SIM, TIRF-SIM, 3D SIM
Fluorescence Excitation at 405, 488, 561 nm
Software : NIS-Elements

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Microscopes Elements

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Stochastic Optical Reconstruction Microscopy

Starting at $134.40 per hour

N-STORM from Nikon

2D STORM and 3D STORM

Fluorescence excitation :

405, 488, 561, 647 nm

Software : NIS-Elements

N-STORM from Nikon

2D STORM and 3D STORM

Fluorescence excitation :

405, 488, 561, 647 nm

Software : NIS-Elements

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Image Analysis

Starting at $64.00 per hour

Software :

ImageJ/Fiji
Volocity
NIS-Elements
CellProfiler
Matlab
Features :

  • Image Processing
  • Deconvolution
  • Colocalization
  • Volume reconstruction
  • Segmentation
  • Measurements
    ...

Software :

ImageJ/Fiji
Volocity
NIS-Elements
CellProfiler
Matlab
Features :

  • Image Processing
  • Deconvolution
  • Colocalization
  • Volume reconstruction
  • Segmentation
  • Measurements
    ...
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Laser Capture Microdissection

Starting at $134.40 per hour

PALM Microbeam IV from Zeiss

Laser Capture Microdissection And Microirradiation And Color Histology Imaging

  • Automated stage for multi-location time-lapse or mosaic imaging
  • Autofocus using hardware (Definite Focus) or software
  • Non-contact laser catapulting for capturing live cells or material from frozen or fixed... Show more »

PALM Microbeam IV from Zeiss

Laser Capture Microdissection And Microirradiation And Color Histology Imaging

  • Automated stage for multi-location time-lapse or mosaic imaging
  • Autofocus using hardware (Definite Focus) or software
  • Non-contact laser catapulting for capturing live cells or material from frozen or fixed tissue
  • Captured material can be used for isolation of DNA, RNA, or protein, or for cell/tissue culture applications
  • Axiocam ICc for color imaging and histological staining

Software: PALM Robo, Zen

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Multiphoton Fluorescence Microscopy

Starting at $134.40 per hour

Nikon A1RMP Confocal

Features :

  • Motorized XY Stage
  • Autofocus using the Perfect Focus System
  • Tokai Hit stage-top incubator and objective heater
  • 2-photon microscopy with non-descanned detector
  • Nikon 25x/1.10 coverslip-corrected water-immersion IR lens

Fluorescence excitation :

  • 2-photon: Software-tunable Chameleon II laser (680-1040 nm)

Software : NIS-Elements

Nikon A1RMP Confocal

Features :

  • Motorized XY Stage
  • Autofocus using the Perfect Focus System
  • Tokai Hit stage-top incubator and objective heater
  • 2-photon microscopy with non-descanned detector
  • Nikon 25x/1.10 coverslip-corrected water-immersion IR lens

Fluorescence excitation :

  • 2-photon: Software-tunable Chameleon II laser (680-1040 nm)

Software : NIS-Elements

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Spinning Disk Confocal Microscopy

Starting at $134.40 per hour

Yokogawa CSU-X1

Fluorescence excitation:

405, 488, 561, 647 nm

Features:

  • Tokai Hit stage-top incubator and objective heater
  • Motorized Stage and Piezo Z
  • EMCCD Camera (512x512) (Evolve)

Software: NIS-Elements

Yokogawa CSU-X1

Fluorescence excitation:

405, 488, 561, 647 nm

Features:

  • Tokai Hit stage-top incubator and objective heater
  • Motorized Stage and Piezo Z
  • EMCCD Camera (512x512) (Evolve)

Software: NIS-Elements

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Confocal Microscopy

Starting at $134.40 per hour

Nikon A1 Confocal or Nikon A1RMP confocal
Fluorescence Excitation :
405, 458, 488, 514, 561, 639 nm
Features :

  • Motorized XY Stage allowing mosaic images and multi-point time-lapse imaging.
  • Autofocus using the Perfect Focus System.
  • Spectral unmixing of overlapping fluorophores or autofluorescence
  • Tokai Hit stage-top incubator and objective heater
    Software : NIS-Elements

Nikon A1 Confocal or Nikon A1RMP confocal
Fluorescence Excitation :
405, 458, 488, 514, 561, 639 nm
Features :

  • Motorized XY Stage allowing mosaic images and multi-point time-lapse imaging.
  • Autofocus using the Perfect Focus System.
  • Spectral unmixing of overlapping fluorophores or autofluorescence
  • Tokai Hit stage-top incubator and objective heater
    Software : NIS-Elements
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Microscopes Confocal microscope Elements

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DIC Microscopy

Differential interference contrast microscopy
Starting at $134.40 per hour
Request a quote for more information about this service.
Microscopes

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TIRF

Total Internal Reflection Microscopy
Starting at $134.40 per hour

Fluorescence excitation :

488, 561 nm
EM-CCD camera (Andor)
Software : NIS-Elements

Fluorescence excitation :

488, 561 nm
EM-CCD camera (Andor)
Software : NIS-Elements

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Widefield Fluorescence Microscopy

Starting at $134.40 per hour

Inverted Zeiss Axio Observer.Z1

Features:

  • Automated stage for multi-location time-lapse or mosaic imaging
  • Autofocus using hardware (Definite Focus) or software
  • Bioptechs objective heater
  • Fast switching LEDs for multicolor live imaging
  • Orca ER cooled CCD camera

Fluorescence excitation:

Inverted Zeiss Axio Observer.Z1

Features:

  • Automated stage for multi-location time-lapse or mosaic imaging
  • Autofocus using hardware (Definite Focus) or software
  • Bioptechs objective heater
  • Fast switching LEDs for multicolor live imaging
  • Orca ER cooled CCD camera

Fluorescence excitation:

  • Mercury halide lamp with fluorescence filters
  • LEDs at 390, 455, 470, 505 and 590 nm

Software : ZenBlue

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Data Services

Price on request
Request a quote for more information about this service.

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Fluorescence-Based Microscopy

Price on request
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Cells and Tissues

Price on request
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Tissue Processing

Price on request
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Super Resolution Microscopy

Price on request
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Microscopy

Price on request
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Optical Microscopy

Price on request
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Tissue Preparation

Price on request
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Biospecimens

Price on request
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Biology

Price on request
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Imaging & Spectroscopy

Price on request
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Computational Modeling

Price on request
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Transmitted Light Microscopy

Price on request
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Theresa Swayne

Manager

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