The Confocal and Light Microscopy core facility at Dana Farber Cancer Institute provides state-of-the-art imaging microscopy technology for fixed and live cells and tissues along with scientific and technical expertise to assist in experimental design and optimal image collection.
Imaging techniques available through the core include:
Live-cell long term imaging
Fluorescence spectral detection
Fluorescence lifetime imaging
Available equipment includes:
Yokogawa spinning disk confocal microscope (Andor)
Leica SP5 laser scanning confocal with a white light laser
Zeiss 710 confocal/multi-photon
Nikon inverted widefield fluorescence microscope
Training on all equipment, consultation about various imaging techniques, and assistance with image analysis is also available. Core staff is also able to assist with troubleshooting microscope issues in individual research labs.
The DFCI Confocal and Light Microscopy Core Facility has several types of microscopy equipment equipment available for use. We have a widefield (non-confocal) inverted microscope that has both transmitted light and epi-fluorescence illumination which can be used for both fixed cell fluorescence imaging or live-cell imaging. In addition, a spinning disk confocal microscope on an inverted Nikon microscope is available. A Leica SP5 laser scanning confocal on an inverted microscope has conventional scanner, a fast resonant scanner, and spectral excitation and emission. Our newest investment is the Zeiss 710 laser scanning confocal / multi-photon / FLIM system.
Spinning Disk Confocal Assisted Use
$115.00/hr (DFCI internal)
$185.00 (external academic)
Leica SP5X confocal Assisted Use
$130.00/hr (DFCI internal)
$200.00 (external academic)
Zeiss 710 with 2-photon laser or FLIM Assisted Use
$165.00/hr (DFCI internal)
$180.00 (external academic)
The Director is available to discuss your project and imaging needs, help troubleshoot sample preparation, optimize the imaging equipment, or assist with equipment in your own lab.
$90.00/hr (DFCI internal)
$155.00 (external academic)
Help with microscopy equipment that you have in your lab. Services available in your own lab include:
a) Mercury bulb replacement: Mercury bulbs need to be replaced after about 200 hours of use for optimal fluorescence microscopy and to ensure safety. Alignment of the bulb also needs to be checked periodically in order to make sure illumination is even across the field of view. I will replace your bulb and show you proper alignment technique. $45 (not including replacement bulb).
b) Halide/mercury fluorescent bulb replacement: The new fluorescent light sources use bulbs that last longer than the older mercury bulbs and are designed to have even illumination with minimal alignment. Bulbs are rated to last 2000 hrs., but many last for less time. The fluorescent light is delivered via what is called a liquid light guide, which decays over time and also requires periodic replacement. $45 (not including replacement bulb or replacement liquid light guide).
c) Halogen/brightfield bulb replacement: The brightfield / white light halogen bulb on your microscope will periodically burn out. The lifetime of these bulbs is variable and much less critical to monitor than mercury bulbs. These are like typical light bulbs that can just be replaced when they burnout. Alignment should be performed each time a user sits down at the microscope (see Koehler Illumination) $45 (not including replacement bulb).
Koehler illumination tutorial
Microscope misalignment can cause many problems with transmitted light (brightfield, phase, Nomarski DIC) and fluorescence imaging. It is essential that each microscopy user understand and be able to perform Koehler alignment, particularly with transmitted light microscopy since it requires realignment of the light source each time the sample or objective is changed. I will show you how to do this alignment on your microscope. $90 per hour.
Objective lens cleaning
Objective lenses must be cleaned on a regular basis to ensure optimal performance. Even with the best microscope and camera, you cannot capture a good image with a dirty lens! I will carefully clean the objectives on the microscope in your lab. $45 for up to five objectives.
Microscopes require regular maintenance to keep them in good working order. Without proper care, poor image quality and damaged equipment could result. This service includes cleaning the condenser, removing the oil and dust from all of the optics, and checking the performance of instrument. $90 per hour. Email me your microscope model for an estimate.
Diagnose a problem with your microscope
If you are having problems with a microscope in your lab, I may be able to help detect a problem. We can determine whether it’s a quick fix or something that may require contacting the appropriate company. Perhaps your fluorescence image is not what you’d expect? or maybe you are having a problem capturing an image like what you can see through the eye pieces? Please contact me. Help that requires extensive time will cost $90 per hour.
Confocal and Light Microscopy core facility (DFCI) has not received any reviews.
Confocal and Light Microscopy core facility (DFCI) has not received any endorsements.