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Complex Carbohydrate Research Center - Analytical Services

7 Orders Completed
Athens, Georgia, US

About Complex Carbohydrate Research Center - Analytical Services

Divison of: University of Georgia

The University of Georgia Complex Carbohydrate Research Center (CCRC) occupies an 140,000 sq.ft. building specifically designed for the interdisciplinary and equipment-intensive nature of carbohydrate science.

The CCRC’s mission is to optimize cooperation and collaboration among disciplines (biomedical,... Show more »

The University of Georgia Complex Carbohydrate Research Center (CCRC) occupies an 140,000 sq.ft. building specifically designed for the interdisciplinary and equipment-intensive nature of carbohydrate science.

The CCRC’s mission is to optimize cooperation and collaboration among disciplines (biomedical, plant, and microbial glycosciences, synthetic and analytical chemistry) both within the CCRC and with scientists world-wide and to foster analytical service and training.

CCRC Analytical Services offers purification and structural analysis of carbohydrates from animal, plant and bacteria (LPS, CPS and EPS) using a combination of chromatography, mass spectrometry, and NMR spectroscopy techniques. We also offer hands-on courses on carbohydrate analysis on a yearly basis.

We offer a confidential service and although we are not GLP certified, we are committed to follow the GLP and GMP regulations and ICH guidelines. We also develop standard operating procedures (SOPs). We are willing to sign Confidential Disclosure Agreements (CDA) with companies and universities upon request.

Recent Publications

  • RP da Silva, C Heiss, IC Black, P Azadi, JQ Gerlach, LR Travassos, L Joshi, M Kilcoyne, R Puccia. 2015. Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for D-SIGN receptors. Sci Rep 5: 14213-.
  • E Erikson, PR Wratil, M Frank, I Ambiel, K Pahnke, MP Claveria, P Azadi, N Izquierdo-Useros, H Martinez-Oucadi, C Neuer, RI Schnaar, PR Crocker, W Reuter, OT Keppler. 2015. Mouse Siglec-1 mediates trans-infection of surface-bound murine leukemia virus in sialic acid N-acyl side chain-dependent manner. J Biol Chem.
  • M Zhong, H Zhang, JP Reilly, JD Christie, M Ishihara, T Kumagai, P Azadi, MP Reilly. 2015. ABO Blood Group as a Model for Platelet Glycan Modification in Arterial Thrombosis. Arterioscler Thromb Vasc Biol 35(7): 1570-1578.
  • C Wikraiphat, N Saiprom, S Tandhavanant, C Heiss, P Azadi, G Wongsuvan, A Tuanyok, MT Holden, MN Burtnick, PJ Brett, SJ Peacock, N Chantratita. 2015. Colony Morphology Variation of Burkholderia Pseudomallei is Associated with Antigenic Variation and O-Polysaccharide Modifications. Infect Immun 83(5) : 2127-2128.
  • G Suarez, J Romero-Gallo, M Piazuelo, G Wang, R Maier, L Forsberg, P Azadi, M Gomez, P Correa, R Peek. 2015. Modification of helicobacter pylori peptidoglycan enhances NOD1 activation and promotes cancers of the stomach. Journal of Cancer Research 75(8): 1749-1759.
  • N Lin, J Mascarenhas, N Sealover, H George, J Brooks, K Kayser, B Gau, I Yasa, P Azadi, S Archer-Hartmann. 2015. Chinese hamster ovary (CHO) host cell engineering to increase sialylation of recombinant therapeutic proteins by modulating sialytransferase expression. Biotechnol Prog 31(2): 334-346. PMID:25641927
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Our Services (20)


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Glycan Analysis

Price on request
  • Glycosyl composition
  • Glycosyl linkage
  • Molecular weight
  • Glycan profiling
  • 1D and 2D-NMR
  • Mass spectrometry (MALDI-MS, ESI/NSI-MS and MS/MS, LC-ESI-MS, GC-MS)
  • Glycosyl composition
  • Glycosyl linkage
  • Molecular weight
  • Glycan profiling
  • 1D and 2D-NMR
  • Mass spectrometry (MALDI-MS, ESI/NSI-MS and MS/MS, LC-ESI-MS, GC-MS)
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Glycomics Studies

Price on request

We can perform glycomics studies starting from either purified glycoproteins or from cells, tissues, culture supernatants, and gel slices. The analysis can begin on purified animal, plant or bacterial, natural or recombinant glycoproteins, or we can isolate and purify the glycoprotein mixture from cells, tissues, culture... Show more »

We can perform glycomics studies starting from either purified glycoproteins or from cells, tissues, culture supernatants, and gel slices. The analysis can begin on purified animal, plant or bacterial, natural or recombinant glycoproteins, or we can isolate and purify the glycoprotein mixture from cells, tissues, culture supernatants, or gel slices. We can develop custom glycomics services as needed.

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Thermo Fisher Orbitrap Mass Spec Mass Spectrometry

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Glycosaminoglycan Analysis

Price on request

We offer full structural characterization of glycosaminoglycan products and biosimilars such as heparin, low molecular weight heparin, chondroitin sulfate and other GAGs.

Services being offered for GAG structural analysis:
• Enzyme digestion of glycosaminoglycans to disaccharides
• Chemical degradation and HPLC, MS or NMR... Show more »

We offer full structural characterization of glycosaminoglycan products and biosimilars such as heparin, low molecular weight heparin, chondroitin sulfate and other GAGs.

Services being offered for GAG structural analysis:
• Enzyme digestion of glycosaminoglycans to disaccharides
• Chemical degradation and HPLC, MS or NMR analysis
• Oligosaccharide profiling by CTA-SAX-HPLC
• Identification of disaccharides and sulfation pattern by online ESI-MS and ESI-MS/MS
• Identification of released disaccharides by SAX-HPLC, and detection of the disaccharides by both UV and fluorescent detection.
• Structural characterization of GAG oligosaccharides by 1D and 2D NMR
• Sequencing of GAG oligosaccharides by ESI-MS and ESI-MS/MS
• Glycosyl composition analysis of GAGs by GC-MS

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SAX-HPLC GC-MS NMR ESI-MS

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Plant Polysaccharide Analysis

Price on request

Including starch, arabinoxylans, cellulose, chitin and pectins.

  • Purification of plant polysaccharides and isolation of pectin cell wall
  • Glycosyl residue composition analysis
  • Identification of lipid anchors
  • MW determination by size exclusion chromatography with light scattering detector (SEC-ELSD)
  • Chitin analysis
    -... Show more »

Including starch, arabinoxylans, cellulose, chitin and pectins.

  • Purification of plant polysaccharides and isolation of pectin cell wall
  • Glycosyl residue composition analysis
  • Identification of lipid anchors
  • MW determination by size exclusion chromatography with light scattering detector (SEC-ELSD)
  • Chitin analysis
  • Sequencing of polysaccharides by 1D and 2D-NMR analysis
  • Specific test for beta-1,3-glucan analysis
  • Absolute configuration (D or L) of monosaccharides
  • Fatty acid analysis by GC-MS
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GC-MS NMR Analytical SEC

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Lignin Analysis

Price on request

We offer a combination of the following chemical and instrumental analysis for profiling lignocellulosic biomass and pretreated biomass:

  • Pyrolysis Molecular Beam Mass Spectroscopy (py-MBMS): analysis of lignin and C5 and C6 glycosyl compositions in the plant cell wall. Total lignin content, p-hydroxyphenyl (H), syringyl to... Show more »

We offer a combination of the following chemical and instrumental analysis for profiling lignocellulosic biomass and pretreated biomass:

  • Pyrolysis Molecular Beam Mass Spectroscopy (py-MBMS): analysis of lignin and C5 and C6 glycosyl compositions in the plant cell wall. Total lignin content, p-hydroxyphenyl (H), syringyl to guaiacol (S/G/H) ratios can be estimated directly from the spectra.
  • Pyrolysis Gas Chromatography Mass Spectroscopy (py-GCMS): analysis/characterization of lignin structure in plant cell walls.
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py-MBMS py-GCMS Chemometrics

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Lipopolysaccharide Extraction and Purification

Price on request

Isolation methods:

  • for smooth LPS and LOS: hot phenol/water, detergent, EDTA-TEA extraction
  • for rough LPS mutants: phenol-chloroform-petroleum ether method (PCP)
    Extraction methods may need modifications based on the specific nature of the project.

Purification methods:

Isolation methods:

  • for smooth LPS and LOS: hot phenol/water, detergent, EDTA-TEA extraction
  • for rough LPS mutants: phenol-chloroform-petroleum ether method (PCP)
    Extraction methods may need modifications based on the specific nature of the project.

Purification methods:

  • Ultracentrifugation
  • Enzymatic digestion (nucleases, protease)
  • De-lipidation
  • Size exclusion chromatography (SEC) in associative and dissociative conditions
    The progress of purification is verified by SDS-/DOC-PAGE.

Analysis of O-chain and core region released from LPS:
Purification and size determination by FPLC SEC, high-performance anion exchange chromatography (HPAEC) and reverse phase chromatography; monosaccharide composition by GC-MS and GC-FID, GC-CI; glycosyl linkage of neutral and acidic sugars by method of partially methylated alditol acetates (PMAA); glycosyl linkage of of Kdo (2-keto-3-deoxy-octulosonic acid); determination of certain hexosaminuronic acids, deoxyheptulosaric acids, and pseudaminic acid; elucidation of glycosyl sequence and structure by 1D and 2D homo- and heteronuclear NMR, and high resolution MALDI-TOF MS and MS/MS, electrospray ionization (ESI-MS) mass spectrometry.

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MALDI-TOF-MS HPAEC GC-CI GC-MS NMR Analytical SEC SDS-PAGE ESI-MS GC-FID Show 9 more tags Show less

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Capsular Polysaccharide Analysis

Price on request

Isolation and purification of capsular polysaccharides from Gram-negative and Gram-positive bacterial cell; monosaccharide and fatty acid composition by GC-MS and GC-FID, GC-CI; glycosyl linkage analysis by method of partially methylated alditol acetates (PMAA); purification and size determination by FPLC size exclusion... Show more »

Isolation and purification of capsular polysaccharides from Gram-negative and Gram-positive bacterial cell; monosaccharide and fatty acid composition by GC-MS and GC-FID, GC-CI; glycosyl linkage analysis by method of partially methylated alditol acetates (PMAA); purification and size determination by FPLC size exclusion chromatography (SEC), high-performance anion exchange chromatography (HPAEC); structure of polysaccharide and diacylglycerol lipid anchor moieties by MALDI-TOF MS and MS/MS, electrospray ionization (ESI-MS) and by 1D and 2D homo- and heteronuclear NMR.

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MALDI-TOF-MS HPAEC GC-CI GC-MS NMR Analytical SEC ESI-MS GC-FID Show 8 more tags Show less

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Exopolysaccharide Analysis

Price on request

EPSs are isolated form bacterial culture media or from biofilm either in your laboratory or in our facility (please consult specific condition) and extensively purified by using size exclusion chromatography (SEC). EPS can be characterized by molecular size, monosaccharide composition, glycosyl linkage analysis by method of... Show more »

EPSs are isolated form bacterial culture media or from biofilm either in your laboratory or in our facility (please consult specific condition) and extensively purified by using size exclusion chromatography (SEC). EPS can be characterized by molecular size, monosaccharide composition, glycosyl linkage analysis by method of partially methylated alditol acetates (PMAA) and by 1D and 2D homo- and heteronuclear NMR; Comprehensive structural characterization often requires enzymatic depolymerization of EPS.

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GC-MS NMR Analytical SEC

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NMR

Nuclear magnetic resonance spectroscopy
Price on request

We offer routine 1D-1H NMR and 13C-NMR spectroscopy as well as 2D-NMR experiments such as COSY, TOCSY, HSQC.

Nuclear Magnetic Resonance (NMR) instruments:

  • 300 MHz (2 channel, gradients)
  • 500 MHz (89mm wide-bore magnet for solids and liquids, 3 channel, gradients)
  • 600 MHz (3 channel, gradient)
  • 800 MHz (3 channel, gradients)

We offer routine 1D-1H NMR and 13C-NMR spectroscopy as well as 2D-NMR experiments such as COSY, TOCSY, HSQC.

Nuclear Magnetic Resonance (NMR) instruments:

  • 300 MHz (2 channel, gradients)
  • 500 MHz (89mm wide-bore magnet for solids and liquids, 3 channel, gradients)
  • 600 MHz (3 channel, gradient)
  • 800 MHz (3 channel, gradients)
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Protein Characterization

Price on request
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Glycosylation Analysis

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Biochemistry & Molecular Biology

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Imaging & Spectroscopy

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Computational Modeling

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Protein Sequencing

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Spectroscopy

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Carbohydrate Services

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Data Services

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Biology

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Protein Services

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Parastoo Azadi

Technical Director
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Christian Heiss

Assistant Technical Director
  • Positive review for Glycan Analysis:

    November 2016

    "It is the second time for me to deal with CCRC labs. For any researcher, the most important thing is to be sure that the place he/she chose to process his/her samples has experts to process samples, analyze and interpret data. Really , all these requirements were fulfilled in CCRC. Really, they are expertise. I recommend any researcher worldwide to contact CCRC for processing any carbohydrate sample with absolute confidence and full guarantee of obtaining valid consistent reproducible results. They respond to all my inquiries and give me complete explanation for my inquiries. It is rare to find well qualified lab such as CCRC to satisfy all your scientific requirements in sample processing and data analysis. Great thankfulness and appreciation for CCRC staff members for the great experience in the field of carbohydrate. Special thankfulness and appreciation for Dr. Parastoo Azadi and Dr. Christian Heiss for the great experience in the field of carbohydrate. I hope to rework and repeat my experience with CCRC again and agian. Great thankfulness for the team work of Science Exchange for giving me a chance to know CCRC. Special great thankfulness for Ms. Melissa Songpitak, a highly active member in Science Exchange team for her continuous help , support in follow up the orders."

  • Positive review for NMR:

    October 2016

    "It is the first time for me to have an order in CCRC. I got a detailed report on NMR results for my samples processed in CCRC as i asked the Service Provider from the beginning of this order. Moreover, i got a detailed replies on all my inquiries about my NMR results for the samples. I can tell other researchers that CCRC have staff members with high experiences in NMR analyses for carbohydrates. I greatly recommend other researchers to perform their NMR analyses for their carbohydrate samples in CCRC. I greatly appreciate the great effort done for order completion by CCRC staff members. Special thankfulness for Dr. Parastoo Azadi and Dr. Christian Heiss for scientific support and getting results with high validity and perfection. . I greatly appreciate the great effort did by staff members of Science Exchange to help researchers allover the world to find centers and labs with high expertise to carry out their lab works concerning researches. Special thankfulness for Ms. Melissa Spongpitak, a highly active and energetic member in the Science Exchange team for her continuous help, support, guide and interest from the date of order initiation to order completion."

Complex Carbohydrate Research Center - Analytical Services has not received any endorsements.