"Overall, this was a disappointing experience with Comparative Biosciences. The most surprising thing to me was the complete lack of scientific curiosity on the part of the scientists at this company. It felt like the vendor's chief goals were to churn through the steps of the experiment without regard to the question being asked or the quality of data being delivered. I had asked Comparative Biosciences to conduct a pilot IHC experiment with a new human antibody that were are testing as a therapeutic. As part of the protocol, they agreed to "optimization of staining method for huIgG," then dosing mice and staining tissues for the test material after dosing. After staining, they found that the test material was not found in the mouse tissue and concluded that the test material did not enter the tissue. When asked for controls to demonstrate that their staining method works, they sent a micrograph showing that the secondary antibody they used was able to stain human spleen. Let me repeat: their "optimization" involved staining 1) irrelevant human tissue with 2) only the secondary antibody (without the test material). When asked how they know the secondary antibody binds to my test material, they said that they don't know the epitope of the test material and therefore cannot test it on mouse sections. After a few rounds of back and forth, it was clear they did not understand why staining human tissues with an anti-human secondary antibody was not a good control for IHC in mouse tissues stained with a foreign antigen. I eventually got tired of coaching the scientist and making experimental suggestions."
Comparative Biosciences, Inc. has not received any endorsements.