1346303525000

Clinical Microarray Core

Los Angeles, California, 90095
1346303525000
Clinical Microarray CoreUniversity of California, Los AngelesLos Angeles, California, 90095
  • 23 Services
  • 2 Members

UCLA Clinical Microarray Core/JCCC Genomics Shared Resource (CMC/GSR), directed by Dr. Xinmin Li, including a staff of six with 56 years combined genomics experience. [http://youtu.be/9gEONcrFDDk] We are a pharma trial site for Pfizer and Quintiles. This CLIA/CAP genomic facility equipped with all major next generation sequencing/microarray instruments and related bioinformatics resources including:

 Nucleic acids isolation and QC
o MagNa Pure Compact
o Applied Biosystems 7500 Fast Real
o Agilent 2100 Bioanalyzer, NanoDrop 8000, Qubit 2.0 Fluorometer

 NGS
o Illumina HiSeq2500 sequencing system
o Illumina MiSeq sequencing system
o Ion Personal Genome Machine (PGMTM)
o IntegenX Apollo 324 system
o Fluidigm Access Array™ System

 Microarray
o Affymetrix GeneChip® System 3000Dx v.2
o Affymetrix... Show more »

UCLA Clinical Microarray Core/JCCC Genomics Shared Resource (CMC/GSR), directed by Dr. Xinmin Li, including a staff of six with 56 years combined genomics experience. [http://youtu.be/9gEONcrFDDk] We are a pharma trial site for Pfizer and Quintiles. This CLIA/CAP genomic facility equipped with all major next generation sequencing/microarray instruments and related bioinformatics resources including:

 Nucleic acids isolation and QC
o MagNa Pure Compact
o Applied Biosystems 7500 Fast Real
o Agilent 2100 Bioanalyzer, NanoDrop 8000, Qubit 2.0 Fluorometer

 NGS
o Illumina HiSeq2500 sequencing system
o Illumina MiSeq sequencing system
o Ion Personal Genome Machine (PGMTM)
o IntegenX Apollo 324 system
o Fluidigm Access Array™ System

 Microarray
o Affymetrix GeneChip® System 3000Dx v.2
o Affymetrix GeneChip® System 3000
o Affymetrix GeneAtlas system
o Agilent microarray system
o Roche NimbleGen microarray system
o miRNA array system
o Beckman Coulter ArrayPlex

 Bioinformatics
o Hoffman2 data storage server
o Hoffman2 Linus clusters equipped with various genomic data analysis tools
including Galaxy server
o Lab-hosted data analysis server and data analysis tools including Partek
Genomics Suite, NextGENe, Ingenuity.

With the state of the art instruments and well established data analysis and management pipelines, the CMC/GSR offers a wide range of genomic services including:

 Automated DNA/RNA isolation & quality evaluation

 Next generation sequencing services
o RNA-Seq
o miRNA-Seq
o Whole exon sequencing
o Targeted resequencing
o Methy-Seq
o ChIP-Seq
o CLIP-Seq

 Microarray serviceso Gene expression profiling
o SNP genotyping & CNV analysis
o Global methylation analysis
o ChIP-Chip analysis
o miRNA profiling

 Bioinformatics services
o NGS/Microarray Data analysis
o Data analysis consultation & software tutorial
o Online data delivery & long-term data storage

These state-of-the-art NGS and microarray services are offered in a cost-effective and timely manner to basic science, translational and clinical researchers.

The CMC/GSR clientele include researchers generally unfamiliar with whole genome and bioinformatics approaches as well as experts seeking more sophisticated solutions.

In addition to providing comprehensive genomic services, the CMC/GSR provides “beyond service” assistance in science and technology which includes fostering new ideas, facilitating project collaboration and integration across biomedical disciplines and developing new applications to advance the quality of the science.

The CMC/GSR also provides educational training to faculty, staff and students and strives to raise awareness of new directions and major discoveries in the areas of genomics and bioinformatics.

By providing the above services, the CMC/GSR has supported a wide range of translational investigations, including:

 Evaluation of drug effect
 Optimization of drug dose
 Identification of drug targets
 Identification of expression signature for disease classification
 Identification of gene expression markers for disease diagnosis/prognosis
 Evaluation of host response to pathogen infection
 Identification of disease-associated SNPs
 Identification of pathogenic copy number variations
 Understanding molecular mechanisms of pathogenesis and evolution

Sample requirements:
DNA minimum is 1 ng, HIGH quality
or
RNA Standard protocol - 1 ug
RNA specialized protocol - 500pg

In summary, the CMC/GSR provides next-generation sequencing (NGS)/microarray platforms, an integrated bioinformatics pipeline and technical/intellectual expertise, which has significantly facilitated scientific findings and yielded numerous high impact publications including:

 Bensinger SJ, Bradley MN, Joseph SB, Zelcer N, Janssen EM, Hausner MA, Shih R, Parks JS, Edwards PA, Jamieson BD, Tontonoz P. LXR signaling couples sterol metabolism to proliferation in the acquired immune response. Cell. 2008;134:97-111. PMC2626438

 Burton JB, Johnson M, Sato M, Koh SB, Mulholland DJ, Stout D, Chatziioannou AF, Phelps ME, Wu H, Wu L. Adenovirus-mediated gene expression imaging to directly
detect sentinel lymph node metastasis of prostate cancer. Nat Med. 2008;14:882-8. PMC2811163

 Chang J, Wang Z, Tang E, Fan Z, McCauley L, Franceschi R, Guan K, Krebsbach PH, Wang CY. Inhibition of osteoblastic bone formation by nuclear factor-kappaB.
Nat Med. 2009;15:682-9. PMC2768554  Chin MH, Pellegrini M, Plath K, Lowry WE. Molecular analyses of human induced pluripotent stem cells and embryonic stem cells. Cell Stem Cell. 2010;7:263-9. PMC3276111

 Cokus SJ, Feng S, Zhang X, Chen Z, Merriman B, Haudenschild CD, Pradhan S, Nelson SF, Pellegrini M, Jacobsen SE. Shotgun bisulphite sequencing of the
Arabidopsis genome reveals DNA methylation patterning. Nature. 2008;452:215-9. PMC2377394

 Fan Z, Yamaza T, Lee JS, Yu J, Wang S, Fan G, Shi S, Wang CY. BCOR regulates mesenchymal stem cell function by epigenetic mechanisms. Nat Cell Biol. 2009;11:1002-9. PMC2752141

 Ferrari R, Pellegrini M, Horwitz GA, Xie W, Berk AJ, Kurdistani SK. Epigenetic reprogramming by adenovirus e1a. Science. 2008;321:1086-8. PMC2693122

 Nazarian R, Shi H, Wang Q, Kong X, Koya RC, Lee H, Chen Z, Lee MK, Attar N, Sazegar H, Chodon T, Nelson SF, McArthur G, Sosman JA, Ribas A, Lo RS. Melanomas acquire resistance to B-RAF(V600E) inhibition by RTK or N-RAS upregulation. Nature. 2010;468:973-7. PMC3143360

 Poulikakos PI, Persaud Y, Janakiraman M, Kong X, Ng C, Moriceau G, Shi H, Atefi M, Titz B, Gabay MT, Salton M, Dahlman KB, Tadi M, Wargo JA, Flaherty KT,
Kelley MC, Misteli T, Chapman PB, Sosman JA, Graeber TG, Ribas A, Lo RS, Rosen N, Solit DB. RAF inhibitor resistance is mediated by dimerization of aberrantly spliced BRAF(V600E). Nature. 2011;480:387-90. PMC3266695

 Sridharan R, Tchieu J, Mason MJ, Yachechko R, Kuoy E, Horvath S, Zhou Q, Plath K. Role of the murine reprogramming factors in the induction of pluripotency. Cell. 2009;136:364-77. PMC3273494

 Wu X, Ye Y, Kiemeney LA, Sulem P, Rafnar T, Matullo G, Seminara D, Yoshida T, Saeki N, Andrew AS, Dinney CP, Czerniak B, Zhang ZF, Kiltie AE, Bishop DT,
Vineis P, Porru S, Buntinx F, Kellen E, Zeegers MP, Kumar R, Rudnai P, Gurzau E, Koppova K, Mayordomo JI, Sanchez M, Saez B, Lindblom A, de Verdier P, Steineck
G, Mills GB, Schned A, Guarrera S, Polidoro S, Chang SC, Lin J, Chang DW, Hale KS, Majewski T, Grossman HB, Thorlacius S, Thorsteinsdottir U, Aben KK, Witjes
JA, Stefansson K, Amos CI, Karagas MR, Gu J. Genetic variation in the prostate stem cell antigen gene PSCA confers susceptibility to urinary bladder cancer. Nat Genet.
2009;41:991-5. PMC3313685

 Shi H, Moriceau G, Kong X, Lee MK, Lee H, Koya RC, Ng C, Chodon T, Scolyer RA, Dahlman KB, Sosman JA, Kefford RF, Long GV, Nelson SF, Ribas A, Lo RS.
Melanoma whole-exome sequencing identifies (V600E)B-RAF amplificationmediated acquired B-RAF inhibitor resistance. Nat Commun. 2012;3:724.

 Tanaka H, Kono E, Tran CP, Miyazaki H, Yamashiro J, Shimomura T, Fazli L, Wada R, Huang J, Vessella RL, An J, Horvath S, Gleave M, Rettig MB, Wainberg ZA, Reiter RE. Monoclonal antibody targeting of N-cadherin inhibits prostate cancer growth, metastasis and castration resistance. Nat Med. 2010;16:1414-20. PMC3088104

 Tchieu J, Kuoy E, Chin MH, Trinh H, Patterson M, Sherman SP, Aimiuwu O, Lindgren A, Hakimian S, Zack JA, Clark AT, Pyle AD, Lowry WE, Plath K. Female human iPSCs retain an inactive X chromosome. Cell Stem Cell. 2010;7:329-42. PMC2935700

« Show less
    Affymetrix DNA microarray
    $550.00 USD
    per Sample

    Geneatlas Gene Array Hybridization: $550 / sample
    Human U133plus2.0 Array Hybridization: $650 / sample
    Mouse 430 2.0 Array Hybridization: $750 / sample
    Rat 230 2.0 array hybridization: $750 / sample
    Cytoscan HD: $850 / sample
    Other Array Hybridization: pricing on request

    Turnaround time: 2-3... Show more »

    Geneatlas Gene Array Hybridization: $550 / sample
    Human U133plus2.0 Array Hybridization: $650 / sample
    Mouse 430 2.0 Array Hybridization: $750 / sample
    Rat 230 2.0 array hybridization: $750 / sample
    Cytoscan HD: $850 / sample
    Other Array Hybridization: pricing on request

    Turnaround time: 2-3 weeks

    Sample requirements:
    DNA minimum is 1 ng, HIGH quality

    « Show less
    Affymetrix RNA microarray
    $550.00 USD
    per Sample

    Geneatlas Gene Array Hybridization: $550 / sample
    Human U133plus2.0 Array Hybridization: $650 / sample
    Mouse 430 2.0 Array Hybridization: $750 / sample
    Rat 230 2.0 array hybridization: $750 / sample
    Cytoscan HD: $850 / sample
    Other Array Hybridization: pricing on request

    Turnaround time: 2-3... Show more »

    Geneatlas Gene Array Hybridization: $550 / sample
    Human U133plus2.0 Array Hybridization: $650 / sample
    Mouse 430 2.0 Array Hybridization: $750 / sample
    Rat 230 2.0 array hybridization: $750 / sample
    Cytoscan HD: $850 / sample
    Other Array Hybridization: pricing on request

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    « Show less
    Affymetrix microRNA microarray
    $550.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    « Show less
    Agilent DNA microarray
    $450.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    DNA minimum is 1 ng, HIGH quality

    Turnaround time: 2-3 weeks

    Sample requirements:
    DNA minimum is 1 ng, HIGH quality

    « Show less
    Agilent RNA microarray
    $450.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    « Show less
    Agilent microRNA microarray
    $450.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    « Show less
    Exiqon microRNA microarray
    $750.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    « Show less
    Genomic DNA extraction
    $42.00 USD
    per Sample

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    Fees:

    -Nucleic Acid Isolation - Automated
    Unit Price: $50 JCCC rate: $42

    -Nucleic... Show more »

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    Fees:

    -Nucleic Acid Isolation - Automated
    Unit Price: $50 JCCC rate: $42

    -Nucleic Acid Isolation - From FFPE Sample
    Unit Price: $50 JCCC rate: $42

    -Nucleic Acid Isolation - Manual
    Unit Price: $50 JCCC rate: $42

    Turnaround time: 2-3 weeks

    « Show less
    Illumina NGS
    Illumina next generation sequencing
    $1,594.00 USD
    per Sample

    HiSeq 2000 SBS sequencing v3 SR 1 x 50: $1594/lane
    HiSeq 2000 SBS sequencing v3 PE 2 x 100: $2389/lane
    MiSeq Sequencing: pricing on request

    Equipment:
    -Illumina HiSeq 2000 Sequencing System
    -Illumina HiSeq 2500 Sequencing System
    -Illumina MiSeq Personal Sequencer System

    Turnaround time:... Show more »

    HiSeq 2000 SBS sequencing v3 SR 1 x 50: $1594/lane
    HiSeq 2000 SBS sequencing v3 PE 2 x 100: $2389/lane
    MiSeq Sequencing: pricing on request

    Equipment:
    -Illumina HiSeq 2000 Sequencing System
    -Illumina HiSeq 2500 Sequencing System
    -Illumina MiSeq Personal Sequencer System

    Turnaround time: 2-3 weeks

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    « Show less
    Ion Torrent NGS
    Ion Torrent next generation sequencing
    $878.00 USD
    per Sample

    Apliseq Cancer Panel Sequencing $878/sample
    Targeted Resequencing Sequencing $878/sample

    Equipment:
    -Ion Torrent PGM for research applications
    -Ion Torrent PGM for clinical applications

    Turnaround time: 2-3 weeks

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    Apliseq Cancer Panel Sequencing $878/sample
    Targeted Resequencing Sequencing $878/sample

    Equipment:
    -Ion Torrent PGM for research applications
    -Ion Torrent PGM for clinical applications

    Turnaround time: 2-3 weeks

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    « Show less
    Microarray data analysis
    $100.00 USD
    per Hour

    Turnaround time: 2-3 weeks

    Turnaround time: 2-3 weeks

    « Show less
    NGS
    Next Generation Sequencing
    $450.00 USD
    per Sample

    UCLA Clinical Microarray Core offers a wide range of next generation sequencing services including:
    o RNA-Seq
    o miRNA-Seq
    o Whole exon sequencing
    o Targeted resequencing
    o Methy-Seq
    o ChIP-Seq
    o CLIP-Seq
    o NGS/Microarray Data analysis
    o Data analysis consultation... Show more »

    UCLA Clinical Microarray Core offers a wide range of next generation sequencing services including:
    o RNA-Seq
    o miRNA-Seq
    o Whole exon sequencing
    o Targeted resequencing
    o Methy-Seq
    o ChIP-Seq
    o CLIP-Seq
    o NGS/Microarray Data analysis
    o Data analysis consultation & software tutorial
    o Online data delivery & long-term data storage

    These NGS services are offered in a cost-effective and timely manner to basic science, translational and clinical researchers.

    $750 is for one single sample. Practically, people pay around $450/sample.

    Turnaround time: 2-3 weeks

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    « Show less
    NGS data analysis
    Next generation sequencing data analysis
    $100.00 USD
    per Hour

    Turnaround time: 2-3 weeks

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    Turnaround time: 2-3 weeks

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    « Show less
    NimbleGen exon sequencing
    $462.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Turnaround time: 2-3 weeks

    « Show less
    NuGen Ovation Ultralow Library Prep
    $394.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Turnaround time: 2-3 weeks

    « Show less
    NuGen RNA-seq library preparation
    $478.00 USD
    per Sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    « Show less
    RNA extraction
    $42.00 USD
    per Sample

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    Fees:

    -Nucleic Acid Isolation - Automated
    Unit Price: $50 JCCC rate: $42

    -Nucleic... Show more »

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    Fees:

    -Nucleic Acid Isolation - Automated
    Unit Price: $50 JCCC rate: $42

    -Nucleic Acid Isolation - From FFPE Sample
    Unit Price: $50 JCCC rate: $42

    -Nucleic Acid Isolation - Manual
    Unit Price: $50 JCCC rate: $42

    Turnaround time: 2-3 weeks

    « Show less
    RNA sequencing
    $750.00 USD
    per Sample

    RNA-Seq (SR1 x 50): $750 / sample
    RNA-Seq (PR2 x 100): $986 / sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    RNA-Seq (SR1 x 50): $750 / sample
    RNA-Seq (PR2 x 100): $986 / sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Deliverables:
    - Fastq file
    - if it is data analysis, file type will depend on researcher

    « Show less
    Real time reverse transcription polymerase chain reaction (qRT-PCR)
    $208.00 USD
    per Sample

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    QRT-PCR Analysis/Gene - First pair of samples
    Unit price: $208
    JCCC User: $173

    QRT-PCR... Show more »

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    QRT-PCR Analysis/Gene - First pair of samples
    Unit price: $208
    JCCC User: $173

    QRT-PCR Analysis/Gene - Additional pair of samples
    Unit price: $30
    JCCC User: $25

    Platforms:
    - 2X Life Technologies PDM

    Turnaround time: 2-3 weeks

    « Show less
    Truseq DNA library preparation
    $325.00 USD
    per Sample

    Next Generation Sequencing

    Turnaround time: 2-3 weeks

    Sample requirements:
    DNA minimum is 1 ng, HIGH quality

    Next Generation Sequencing

    Turnaround time: 2-3 weeks

    Sample requirements:
    DNA minimum is 1 ng, HIGH quality

    « Show less
    Truseq RNA library preparation
    $349.00 USD
    per Sample

    Next Generation Sequencing

    TruSeq RNA Library prep $349/sample
    TruSeq Small RNA Library prep $429/sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    Next Generation Sequencing

    TruSeq RNA Library prep $349/sample
    TruSeq Small RNA Library prep $429/sample

    Turnaround time: 2-3 weeks

    Sample requirements:
    RNA Standard protocol - 1 ug
    RNA specialized protocol - 500pg

    « Show less
    Whole genome seq
    Whole genome sequencing
    Price on request

    Turnaround time: 2-3 weeks

    Turnaround time: 2-3 weeks

    « Show less
    qPCR
    Quantitative PCR
    $30.00 USD
    per Sample

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    QRT-PCR Analysis/Gene - First pair of samples
    Unit price: $208
    JCCC User: $173

    QRT-PCR... Show more »

    Our core facility provides quality control (QC) services for RNA, miRNA, and DNA samples. This includes RNA/DNA extraction; RNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.

    QRT-PCR Analysis/Gene - First pair of samples
    Unit price: $208
    JCCC User: $173

    QRT-PCR Analysis/Gene - Additional pair of samples
    Unit price: $30
    JCCC User: $250

    Platforms:
    - 2X Life Technologies PDM

    Turnaround time: 2-3 weeks

    « Show less

Anne De Jong gave a positive review for Illumina NGS:

January 07, 2014
We analyzed the NGS data and are very happy with the speed of delivery and quality of the data generated by the UCLA Clinical Microarray Core. For NGS we would definitely choose UCLA again.

Maximilian Larena gave a positive review for RNA sequencing:

January 16, 2014
Highly recommended!
Excellent service at a reasonable price.
jonas-ungerback photo

Jonas Ungerbäck endorsed this lab:

February 25, 2014

My current supervisor, Mikael Sigvardsson, professor in Experimental Hematology, LInköping University, Sweden, mediated the contact since we needed help to analyze small amounts of DNA with ChIP-seq.

The staff has been very helpful and they are also extremely skilled at what they do since library preparation and deep sequencing from DNA amounts to small to measure with standard methods. Moreover, the service has been fast provided and during all the time there has been a very good communication.

As mentioned above I have extremelu well appreciated the experience to work with the Clinical Microarray Core and I have, as a customer felt very important and well handled. Moreover, I feel that I work with very good scientists that take their job very serious and I would therefore any day of the week recommend the Clinical Microarray Core at UCLA to anyone who are in need of such service.

Jonas Ungerbäck, Ph.D, Linköping University, Sweden.

ben-van-handel photo

Ben Van Handel endorsed this lab:

July 30, 2013

I have worked with the CMC for the past 5 years. Dr. Li and Jamie Zhou are absolutely indispensable when it comes to performing microarray or NGS experiments. Consistently, the service they provide is honest, expedient and of the highest quality. On a few occasions, the samples I submitted were of too poor quality to be used; Dr. Li and Jamie immediately contacted me to know how I wished to proceed, thus avoiding wasted time and money. On a more personal note, they and the CMC staff are just wonderful people to work with that will go to the ends of the Earth to help generate quality data. This dedication and passion are rare qualities and should be treasured; I will continue to work exclusively with Dr. Li, Jamie and the CMC as long and I have sequencing needs!

ricardo-frausto photo

Ricardo Frausto endorsed this lab:

July 29, 2013

I am a Senior Research Associate in the Cornea Genetics Laboratory at UCLA, directed by Dr. Anthony J. Aldave. We have used the UCLA Clinical Microarray Core (CMC) services on numerous occasions over the last two years.

We've had RNA and DNA processed through the core and have utilized a number of their technology platforms. For gene expression we used the Affymetrix 1.1ST arrays. We've also used the Affymetrix CytoScanHD array for both copy number analysis and genotyping, the latter of which we are using for relatedness analysis between supposed unrelated individuals. Lastly, we've utilized their DNA-seq capabilities (Illumina Hi-Seq) for both whole exome and region capture sequencing. We plan on using their services for RNA-seq in the near future. All of the data that we've received has been of the highest quality. Dr. Li and his staff have also been very helpful with questions that we have had, both before pursuing a particular approach and after receiving the data.

We've also attended two courses on data analysis and a seminar on utilizing third-party software for the analysis of our data. These have been instrumental in our ability to analyze our data in a manner that allows us to make the most biologically meaningful conclusions.

Probably most importantly, the data received from the core are directly leading to the publication of important findings into the genetics and functional processes underpinning the development of several corneal dystrophies.

I have been most impressed with Dr. Li's willingness to sit down with me to discuss experimental design, so that we are making the most experimentally valid and cost-effective choices. I highly recommend the UCLA Clinical Microarray Core

Endorse this lab