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CIEnP - Center of Innovation and Preclinical Studies

Florianópolis, BR

CIEnP (Center of Innovation and Preclinical studies) is a private and nonprofit Contract Research Organization.

CIEnP collaborates with Sponsors around the world to achieve project objectives, providing clients with high-quality and cost-effective research project.

Services

CIEnP has a number of resources and capabilities available to support your company through the drug discovery and drug development process. Complete support services including consultation, protocol development, quality assurance, analytical chemistry and archive facilities are integral components of studies performed at CIEnP.

Besides our efficacy models and target validation studies we also offer a number of preclinical regulated studies to accomplish the internationals regulatory requirements (FDA, EMA, and ANVISA), following well accepted guidelines (OECD and ICH).

All our Study Directors have either Ph.D degrees and... Show more »

CIEnP (Center of Innovation and Preclinical studies) is a private and nonprofit Contract Research Organization.

CIEnP collaborates with Sponsors around the world to achieve project objectives, providing clients with high-quality and cost-effective research project.

Services

CIEnP has a number of resources and capabilities available to support your company through the drug discovery and drug development process. Complete support services including consultation, protocol development, quality assurance, analytical chemistry and archive facilities are integral components of studies performed at CIEnP.

Besides our efficacy models and target validation studies we also offer a number of preclinical regulated studies to accomplish the internationals regulatory requirements (FDA, EMA, and ANVISA), following well accepted guidelines (OECD and ICH).

All our Study Directors have either Ph.D degrees and between 10-15 years experience, most have been trained worldwide, delivering high-quality research in our core areas.

• PK/TK (in vivo), ADME (in vitro) and bioanalysis
• Efficacy studies for the different systems (gastrointestinal, central and peripheral nervous system, genitourinary/renal, cardiovascular, endocrine);
• Safety (toxicology, safety pharmacology and genotoxicity)
• Cosmetics
• Cell based assays

Contact the CIEnP so that we can assist your company in the development of drugs and cosmetics.

Recent Publications

• Jakubek, l. M. ; Ulinski, G. ; LEITE, DFP ; Slavsky, M. ; Rajanna, S. ; Morelli, J. ; Kelly, M. ; Dwyer, J. ; Fitzgerald, M. ; Piepenhagen, P. ; Jayyosi, Z. . Hepatic Spheroids for Long-term Toxicity Studies. Applied in vitro toxicology, 2016. – Accept for publication.
• Souza PS., Gonçalves ED., Pedroso GS., Farias HR., Junqueira SC.,3, MARCON R., Tuon T., Cola M., Silveira PC., Santos AR., CALIXTO JB., Souza CT,de Pinho RA1, Dutra RC. Physical Exercise Attenuates Experimental Autoimmune Encephalomyelitis by Inhibiting Peripheral Immune Response and Blood-Brain Barrier Disruption, Molecular Neurobiology. Epub ahead of print. (2016). doi:10.1007/s12035-016-0014-0.
• SCHWANK, RC., MARCON, R., BENTO, AF., CALIXTO, JB. EPA- and DHA-derived resolvins' actions in inflammatory bowel disease. European Journal of Pharmacology, v. 785, p 156–164, 2016.
• Forner S, Martini AC, de ANDRADE EL, Rae GA. Neuropathic pain induced by spinal cord injury: Role of endothelin ETA and ETB receptors. Neuroscience Letter. v 23; p 14-21. , 2016.
• Bitencourt, R.M. ; de Souza, A.C. ; Bicca, M.A. ; Pamplona, F.A. ; Mello, N. ; Passos, G.F. ; Medeiros, R.; Takahashi, R.N; CALIXTO, J.B. ; Prediger, R.D. . Blockade of hippocampal bradykinin B1 receptors improves spatial learning and memory deficits in middle-aged rats. Behavioural Brain Research, v. 316, p. 74-81, 2016.
• FREITAS CS., Roveda AC Jr., Truzzi DR., Garcia AC., Cunha TM., Cunha FQ., Franco DW. Anti-inflammatory and Anti-nociceptive Activity of Ruthenium Complexes with Isonicotinic and Nicotinic Acids (Niacin) as Ligands. Journal of Medicinal Chemistry. 58(11), p 4439-48. 2015.
• Loch-Neckel, G ; Bicca, M.A. ; Leal, P.C. ; Mascarello, A. ; SIQUEIRA, J.M. ; CALIXTO, J.B. . In vitro and in vivo anti-glioma activity of a chalcone-quinoxaline hybrid. European Journal of Medicinal Chemistry, v. 90, p. 93-100, 2015.
• Patricio, E S ; Costa, R ; Figueiredo, CP ; Maíra A ; Manjavachi, MN ; SEGAT, GC ; Gentry, C; Luiz, AP ; Fernandes, ES ; Cunha, TM ; Bevan, S; Calixto, JB . Mechanisms Underlying the Scratching Behavior Induced by the Activation of Proteinase Activated Receptor-4 (PAR-4) in Mice. Journal of Investigative Dermatology, v. 135, p. 2484-2491, 2015.
• Luiz, AP ; Schroeder, SD ; RAE, GA ; CALIXTO, JB. ; Chichorro, JG . Contribution and interaction of kinin receptors and dynorphin A in a model of trigeminal neuropathic pain in mice. Neuroscience, v. 14, p. 189-200, 2015.
• Dutra, R.C. ; Bicca, M.A. ; SEGAT, G.C. ; Silva, K.A.B.S. ; Motta, E.M. ; Pianowski, L.F. ; Costa, R. ; CALIXTO, J.B. . The antinociceptive effects of the tetracyclic triterpene euphol in inflammatory and neuropathic pain models: The potential role of PKCε. Neuroscience, v. 303, p. 126-137, 2015

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CIEnP - Center of Innovation and Preclinical Studies has not listed any services.

Xenograft Development
Price on request

Ectopic tumor xenograft model

Evaluated parameters:
* Body weight
* Tumor volume
* Tumor markers

Ectopic tumor xenograft model

Evaluated parameters:
* Body weight
* Tumor volume
* Tumor markers

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Syngeneic (Allograft) Tumor Models
Price on request

Syngeneic tumor model

Evaluated parameters:
* Body weight
* Final tumor volume
* Tumor markers
* Immune system

Syngeneic tumor model

Evaluated parameters:
* Body weight
* Final tumor volume
* Tumor markers
* Immune system

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Orthotopic Tumor Animal Models
Price on request

Orthotopic tumor xenograft model

Evaluated parameters:
* Body weight
* Final tumor volume
* Tumor markers

Orthotopic tumor xenograft model

Evaluated parameters:
* Body weight
* Final tumor volume
* Tumor markers

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In vitro Oncology Models
Price on request

In vitro assays:

Human and murine tumor cell lines (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cell proliferation
* Apoptosis
* Cell cycle
* Clonogenic assay
* Cell migration
* Mitochondrial membrane potential

In vitro assays:

Human and murine tumor cell lines (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cell proliferation
* Apoptosis
* Cell cycle
* Clonogenic assay
* Cell migration
* Mitochondrial membrane potential

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Physicochemical Testing
Price on request
Request a quote for more information about this service.
In vivo PK/PD Studies
Price on request

Pharmacokinetics and drug metabolism

Pharmacokinetics & drug metabolism studies generates information on absorption, distribution, metabolism and excretion important to the drug development. Those studies help to define and optimize lead compounds; characterize compound profiles; to define the bioavailability of... Show more »

Pharmacokinetics and drug metabolism

Pharmacokinetics & drug metabolism studies generates information on absorption, distribution, metabolism and excretion important to the drug development. Those studies help to define and optimize lead compounds; characterize compound profiles; to define the bioavailability of different formulations; define species-related discrepancies and potential drug interactions; provide valuable data on exposure levels of compounds and their metabolites after single and repeated compound administration, giving support to toxicology studies interpretation;

CIEnP offers:

  • PK studies using unlabeled test compound to assist your drug development plan.

Administration routes: Parenteral, enteral and topic (Single and multiple dosing available)

Matrices: Plasma, serum, whole blood, urine, feces, brain, skin, cerebrospinal fluid (CSF) or other tissues from rodents

Deliverables: Analysis of pharmacokinetics parameters with validated phoenix WinNolinTM software

  • Exploratory assessment of dose proportionality
  • Toxicokinetic studies
  • Development and validation of Bioanalytical methods
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Mouse
Rat
drug metabolism
ADME and DMPK Studies
Drug Metabolism and Pharmacokinetics
Price on request
  • Development and validation of Bioanalytical methods

  • Physicochemical properties determination (Lipophilicity, solubility, pka and stability)

  • Skin Absorption (OECD guideline 428)

  • DMPK in vitro assays (please consult)

  • Development and validation of Bioanalytical methods

  • Physicochemical properties determination (Lipophilicity, solubility, pka and stability)

  • Skin Absorption (OECD guideline 428)

  • DMPK in vitro assays (please consult)

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Cosmetic Product Testing
Price on request

CIEnP offers a comprehensive package of assays to evaluate cosmetic ingredients for their in vitro efficacy and safety. Relatively simple one-cell type monolayer based test methods present a good and cost-efficient starting point. Most cosmetic formulation ingredients will come in contact with skin keratinocytes, melanocytes and... Show more »

CIEnP offers a comprehensive package of assays to evaluate cosmetic ingredients for their in vitro efficacy and safety. Relatively simple one-cell type monolayer based test methods present a good and cost-efficient starting point. Most cosmetic formulation ingredients will come in contact with skin keratinocytes, melanocytes and possibly fibroblasts; therefore all cell types have been established as common in vitro test systems *.

Skin Absorption:

Skin absorption tests aims to understand the percutaneous absorption following topical exposure of your individual ingredients / chemicals or finished products using our skin absorption assay. CIEnP skin absorption test is performed using human skin. Synthetic membranes can also be employed in Franz cell drug diffusion studies. CIEnP skin absorption test can assess a range of non-radiolabelled test articles, including liquids, solids, semi-solids, pastes, gels, and creams.

Toxicity:

Cytotoxicity tests assess the skin irritation potential on cultured human or mammalian cell lines. Cytotoxicity is the first test to provide reliable insight into the safety of cosmetics and chemicals. Toxic effects on the cellular level could be identified by different viability tests including:
* Neutral Red Uptake (NRU)
* MTT Assay
* Anexin and 7AAD

Efficacy in vitro testing on cosmetics and ingredients for supporting for marketing claims:

Anti-aging:

  • Antioxidant activity (DPPH);
  • Measurement of intracellular reactive oxygen species (ROS);
  • Measurement of glutathione levels;
  • Lipid peroxidation assay;
  • Apoptosis;
  • Cell proliferation assay;
  • Cell Cycle regulation;
  • Evaluation of extracellular matrix proteins and metalloproteinases;
  • DNA protection;

Moisturization:

  • Determination of acid hyaluronic;
  • Aquaporin production;

Vitalizing effect:

  • Cell proliferation assay;
  • Cell migration assay;
  • Acid hyaluronic and other matrix protein measurement;
  • ATP determination;
  • Metabolic activity measurement;

Calming effect:

  • Proinflammatory cytokine production/release (IL-1β, IL-6, IL-8, TNF-α);
  • PGE-2 determination;

Skin repair:

  • Expression and activity of Matrix metalloproteinases;
  • Angiogenesis assay;
  • Wound Healing assessment (scratch test on fibroblasts or keratinocytes);
  • Cell migration and proliferation assay;

Antioxidant effect:

  • Measurement of intracellular reactive oxygen species (ROS);
  • Measurement of glutathione levels;
  • Lipid peroxidation assay;
  • Cell proliferation assay;
  • Cell Cycle regulation;
  • Evaluation of extracellular matrix proteins and metalloproteinases;

Firmness:

  • Quantification of skin collagen;
  • Quantification of skin elastin;
  • Quantification of skin fibronectin;
  • Evaluation of matrix metalloproteinases;
  • Cell proliferation assay;

Skin pigmentation:

  • TRP-1, TRP-2, tyrosinase, MC1R and MITF mRNA cell expression;
  • Tyrosinase activity;
  • Melanin content

Techniques:

Western blot;
Real time PCR;
Colorimetric assays;
Enzyme assays;
Immunohistochemistry;
Immunocytochemistry;
Immunofluorescence;
Flow cytometry;

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Skin
Firmness
Skin Absorption
Cytotoxicity
Anti-aging
cosmetic
skin repair
marketing claim
calming effect
firmness
aging
Cell-Based Assays
Price on request

CUSTOMIZED CELL-BASED ASSAY TESTING

CIEnP provides in vitro screening and mechanism of action studies using primary or immortalized cell lines. Our scientific team provides a broad array of validated assays as well as in-depth biology support for drug discovery and we works with sponsor to determinate the best in vitro assay... Show more »

CUSTOMIZED CELL-BASED ASSAY TESTING

CIEnP provides in vitro screening and mechanism of action studies using primary or immortalized cell lines. Our scientific team provides a broad array of validated assays as well as in-depth biology support for drug discovery and we works with sponsor to determinate the best in vitro assay for the proposal.

Example Cell Systems:

  • Pancreas carcinoma – Mia-PaCa-2;
  • Glioblastoma – U87-MG;
  • Neuroblastoma cell (SH-SY5Y);
  • Breast adenocarcinoma – MCF-7;
  • Prostate carcinoma – LNCaP;
  • Lung carcinoma – A549;
  • Colorectal adenocarcinoma – Caco-2;
  • Lymphoblast – TK-6;
  • Keratinocytes;
  • Fibroblasts;
  • Melanocytes;

Example ex vivo cell subsets

  • Bronchiolar lavage cells;
  • Macrophages;
  • Splenocytes;
  • Lymphocytes;
  • Hepatocytes;
  • Primary neuronal cultures from Sprague Dawley rats;
  • Primary glial cultures from Sprague Dawley rats;

Example Readouts:

  • Cytotoxicity;
  • Apoptosis;
  • Cell cycle;
  • Clonogenic assay;
  • Biomarkers (quantitative PCR or ELISA);
  • Cell proliferation;
  • Cell stimulation/activation;
  • Cellular invasion/migration;
  • Cellular phenotyping (multi-color flow cytometry);
  • Mitochondrial membrane potential;
  • Oxidative stress;
  • Morphological characterization;
  • Enzymatic measurements;

The above list is a small sampling of the available assays. Contact a scientist today to discuss a cell-based assay test that can meet your primary objectives.

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Preclinical PK/PD Studies
in vivo Pharmacokinetics/Pharmacodynamics
Price on request

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available, in order to... Show more »

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available, in order to meet the needs of custom assays including:

  • Binding analysis;
  • Boyden chamber;
  • Cistometry;
  • Citology;
  • Flow cytometry;
  • ELISA;
  • calorimetric assay;
  • Enzyme assay;
  • Histology;
  • Immunofluorescence;
  • Microscopy;
  • Tissue/Organ Bath;
  • Real-time PCR;
  • Agonists and antagonists;
  • Western blot;
  • Others (on request).
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Animal Cardiac Test Panel
Price on request

Experimental models:

Carotid Artery and Jugular Vein Catheterization

Parameters Evaluated:
* Mean arterial pressure
* Heart rate

Isolated heart and tissues

Parameters Evaluated:
* Muscle contraction and relaxation
* Intraventricular pressure

Pharmacodynamics

The pharmacodynamic studies are... Show more »

Experimental models:

Carotid Artery and Jugular Vein Catheterization

Parameters Evaluated:
* Mean arterial pressure
* Heart rate

Isolated heart and tissues

Parameters Evaluated:
* Muscle contraction and relaxation
* Intraventricular pressure

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:

  • Western blot;
  • Real time PCR;
  • Colorimetric assays;
  • Enzyme assays;
  • Immunohistochemistry;
  • Immunofluorescence;
  • Organ bath;
  • Experimental surgery;
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Cardiovascular
organ bath
languedorff
cardiovascular
Dermatopathology
Price on request

In vitro assays:

Human fibroblasts and keratinocytes cell lines (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cell proliferation
* Cytokine levels

Experimental models

- Imiquimod-induced psoriasis-like skin inflammation

Evaluated parameters:
* Immunological cell profile
*... Show more »

In vitro assays:

Human fibroblasts and keratinocytes cell lines (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cell proliferation
* Cytokine levels

Experimental models

- Imiquimod-induced psoriasis-like skin inflammation

Evaluated parameters:
* Immunological cell profile
* Scratching behavior
* Skin edema
* Scars
* Skin histology

- Diphenylcyclopropenone (DCP)-induced dermatitis

Evaluated parameters:
* Scratching behavior
* Skin histology

- Itching induced by compound 48/80

Evaluated parameters:
* Scratching behavior
* Skin histology

- Itching induced by receptor PAR-2 agonist

Evaluated parameters:
*Scratching behavior
*Skin histology

Pharmacodynamics:

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:
* Agonists and antagonists;
* Western blot;
* Real time PCR;
* Colorimetric assays;
* ELISA assays;
* H&E stain;
* Immunohistochemistry;
* Immunofluorescence;
* MTT assay;
* Neutral red uptake assay;
* Sulphorhodamine B assay;

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dermatitis
itching
Scratching
psoriasis
Imiquimod-induced psoriasis
Diphenylcyclopropenone- induced dermatitis
Itching induced by compound 48/80
Itching induced by receptor PAR-2 agonist
skin inflammation
dermatology
Gastrointestinal Disease Animal Models
Price on request

In vitro assays:

Intestinal cell line (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cytokine levels
* Permeability

Intestinal inflammation models induced by chemical agents:

Acute or chronic colitis induced by dextran sodium sulfate

In vitro assays:

Intestinal cell line (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cytokine levels
* Permeability

Intestinal inflammation models induced by chemical agents:

Acute or chronic colitis induced by dextran sodium sulfate

  • Acute colitis induced by trinitrobenzene sulfonic acid (TNBS)
  • Acute colitis induced by oxazolone.

Evaluated parameters:
* Lesion extent (macroscopic or microscopic analysis);
* Changes in body weight;
* Clinical signs of colitis;
* Rectal bleeding and diarrhea;
* Detection of neutrophil infiltration;
* Cytokine release;
* Enzymes/proteins activities and expression.

Acute Ulcer models:

  • Nonsteroidal anti-inflammatory drug-induced ulcer (indomethacin, ibuprofen, acetylsalicylic acid);
  • Indomethacin- and bethanechol-induced ulcer;
  • Ethanol-induced ulcer;
  • Cold restraint stress- induced ulcer.

Chronic Ulcer models:

  • Acetic acid-induced ulcer;

Evaluated parameters
* Lesion extent (macroscopic or microscopic analysis);
* Gastric mucus content;
* Reduced glutatione content;
* Detection of neutrophil infiltration;
* Cytokine release;
* Enzymes/proteins activities and expression;
* Gastric mucosa morphology;
* Cell proliferation

Intestinal transit:

  • Activated charcoal labeled intestinal transit model
  • Phenol red labeled intestinal transit model

Evaluated parameters:
* Increase or delay of small intestine transit

Acid gastric secretion evaluation:

  • Pylorus ligature;
  • H+/K+-ATPase activity (in vitro and ex vivo);

Evaluated parameters:
* pH;
* Cytokine release;
* Enzymes activities.

Intestinal constipation:

  • Cold saline-induced model of constipation

Evaluated parameters
* Frequency of defection (per day);
* Faecal water content;
* Measurement of intestinal function;
* Intestinal contractility;
* Enteric nervous system alterations;
* Cytokine release;
* Enzymes/proteins activities and expression.

Gastric emptying:

  • Phenol red labeled gastric emptying model

Evaluated parameters:
* Increase or delay of gastric emptying;

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:
Use of Agonists and antagonists;
*Colorimetric assay;
* Flow cytometry;
* ELISA;
* Histology;
* Immunohistochemistry;
* Immunofluorescence;
* Real-time PCR;
* Western blot;
* Others (on request).

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Mouse
Rat
ulcer
gastric ulcer
colitis
crohn disease
Acid gastric secretion
costipation
Intestinal transit
Gastric emptying
gastroduodenal ulcers
DSS- induced colitis
TNBS- induced colitis
Trinitrobenzenesulfonic acid- induced colitis
Dextran Sulfate Sodium- induced colitis
oxazolone- induced colitis
Ethanol- induced gastric ulcers
NSAID-induced ulcer
indomethacin- induced ulcer
stress- induced ulcers
aspirin-induced ulcer
acetylsalicylic acid- induced ulcer
ASA-induced ulcer
ibuprofen- induced ulcer
Pylorus ligature
H+/K+-ATPase activity
Cold saline-induced model of constipation
Activated charcoal labeled intestinal transit model
Phenol red labeled intestinal transit model
Phenol red labeled gastric emptying model
Phenol red
gastrointestinal
inflammatory Bowel Disease
Rheumatoid Arthritis Animal Models
Price on request

Arthritis and osteoarthritis models

• CFA-induced arthritis;
• Methylated bovine serum albumin (mBSA)-induced arthritis;
• Monosodium iodoacetate-induced osteoarthritis.

Evaluated parameters:
* Changes in body weight;
* Clinical signs of arthritis (score);
* Edema measurement;
* Thermal and mechanical hyperalgesic... Show more »

Arthritis and osteoarthritis models

• CFA-induced arthritis;
• Methylated bovine serum albumin (mBSA)-induced arthritis;
• Monosodium iodoacetate-induced osteoarthritis.

Evaluated parameters:
* Changes in body weight;
* Clinical signs of arthritis (score);
* Edema measurement;
* Thermal and mechanical hyperalgesic threshold;
* Joint microscopic analysis;
* Cell migration (leukocytes);
* Cytokine and other inflammatory mediators release;
* Enzymes/proteins activities and expression.

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:

  • Colorimetric assay;
  • Flow cytometry;
  • ELISA;
  • Histology;
  • Immunohistochemistry;
  • Immunofluorescence;
  • Real-time PCR;
  • Western blot;
  • Others (on request)
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Arthritis
Osteoarthritis (OA)
Monosodium iodoacetate-induced osteoarthritis
CFA-induced arthritis
Methylated bovine serum albumin- induced arthritis
mBSA- induced arthritis
Inflammation Animal Models
Price on request

In vitro assays:

Primary leukocytes and Lymphocytes and macrophages cell lines (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cell proliferation
* Apoptosis
* Cytokine and chemokine production
* Cell migration

For Evaluation Of Analgesic And Anti-Inflammatory Activities Of New... Show more »

In vitro assays:

Primary leukocytes and Lymphocytes and macrophages cell lines (please see Cell-based Assays)

Evaluated parameters:
* Cytotoxicity
* Cell proliferation
* Apoptosis
* Cytokine and chemokine production
* Cell migration

For Evaluation Of Analgesic And Anti-Inflammatory Activities Of New Products.

• Ear edema (arachidonic acid- and croton oil-induced edema);
• Paw edema (carrageenan-, complete Freund's adjuvant (CFA)- and zymosan- induced edema).

Evaluated parameters:
* Edema measurement;
* Plasma extravasation;
* Cell migration (leukocytes);
* Cytokine and other inflammatory mediators release;
* Enzymes/proteins activities and expression.

• Peritonitis (carrageenan-, zymosan- and lipopolysaccharide from Escherichia coli (LPS)- induced peritonitis);
• Pleurisy (carrageenan-, zymosan- and lipopolysaccharide from Escherichia coli (LPS)- induced pleurisy);

Evaluated parameters:
* Plasma extravasation;
* Cell migration (leukocytes);
* Cytokine and other inflammatory mediators release;
* Enzymes/proteins activities and expression.

• Sepsis (cecal ligation and puncture (CLP)- and lipopolysaccharide from Escherichia coli (LPS)- induced sepsis);

Evaluated parameters
* Survival;
* Cell migration (leucocytes);
* Cytokine and other inflammatory mediators release;
* Enzymes/proteins activities and expression.

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:

  • Colorimetric assay;
  • Flow cytometry;
  • ELISA;
  • Histology;
  • Immunohistochemistry;
  • Immunofluorescence;
  • Real-time PCR;
  • Western blot;
    *Others (on request).
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Mouse
Rat
inflammatory
Peritonitis
Sepsis
Pain
pleurisy
Arachidonic acid-induced ear edema
Croton oil-induced ear edema
Carrageenan-induced paw edema
CFA-induced paw edema
Zymosan induced peritonitis
Carrageenan-induced pleurisy
Sepsis induced by CLP
Sepsis induced by LPS
Neuropathic Pain Animal Models
Price on request

Neuropathies

• Partial sciatic nerve ligation- induced neuropathy
• Chemotherapy- induced neuropathy
• Diabetes- induced neuropathy
• Experimental Autoimmune Encephalomyelitis (EAE) induced- neuropathy
• Brachial Plexus Avulsion (BPA)- induced neuropathy

Evaluated parameters:
* Locomotor activity (open field,... Show more »

Neuropathies

• Partial sciatic nerve ligation- induced neuropathy
• Chemotherapy- induced neuropathy
• Diabetes- induced neuropathy
• Experimental Autoimmune Encephalomyelitis (EAE) induced- neuropathy
• Brachial Plexus Avulsion (BPA)- induced neuropathy

Evaluated parameters:
* Locomotor activity (open field, rotarod);
* Hindpaw withdrawal response (mechanical and thermal stimulus);
* Evaluation of the spinal cord;
* Detection of neutrophil infiltration;
* Cytokine release;
* Enzymes/proteins activities and expression;
* Microglial, astrocytes and neurons morphology, transformation, and proliferation.

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:
* Binding analysis;
* Citology;
* Flow cytometry;
* ELISA;
* Colorimetric assay;
* Enzyme assay;
* Histology;
* Immunohistochemistry
* Immunofluorescence;
* Real-time PCR;
* Agonists and antagonists;
* Western blot;
* Others (on request).

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CNS/Neurology
Neuroscience
Neuropathic Pain
neuropathy
Partial sciatic nerve ligation - induced neuropathy
Chemotherapy- induced neuropathy
Diabetes- induced neuropathy
Brachial Plexus Avulsion (BPA)- induced neuropathy
Experimental Autoimmune Encephalomyelitis (EAE) induced- neuropathy
Neurodegeneration Animal Models
Price on request

In vitro Assays (please see Cell-based Assays)

• Primary neuronal and glial cultures isolated from Sprague Dowley rats (E18);
• Glioblastoma cell (U87-MG);
• Neuroblastoma cell (SH-SY5Y)
• Primary dopaminergic neuronal cells;

Evaluated parameters:
* Cytotoxicity
* Cytokine and chemokine production
* Morphological... Show more »

In vitro Assays (please see Cell-based Assays)

• Primary neuronal and glial cultures isolated from Sprague Dowley rats (E18);
• Glioblastoma cell (U87-MG);
• Neuroblastoma cell (SH-SY5Y)
• Primary dopaminergic neuronal cells;

Evaluated parameters:
* Cytotoxicity
* Cytokine and chemokine production
* Morphological characterization;
* Analysis of oxidative stress,
* Enzymatic measurement

Neurodegenerative diseases animal models

• Alzheimer’s disease model (amyloid-β- induced model, Streptozotocin-induced model and scopolamine-induced amnesia model)

Evaluated parameters:
* Behavioral tests (Novel and/or realocation object recognition; and others);
* T-maze; Y-maze; and others)
* Pathologic changes in brains;
* Indicators of oxidative stress;
* Neurotransmitters levels in serum and brain.

• Parkinson’s disease model (MPTP-, Rotenone -, Reserpine-, 6-OHDA- induced models)

Evaluated parameters
* Assessment of the extent of the lesion;
* Screening for motor impairments (open field, rotarod, grip strength,);
* Screening for non-motor symptoms (elevated plus maze, Novel Object Recognition; Social recognition; Elevated plus maze; Forced swim test; Sucrose test; and others)
* Analysis of dopamine (DA) and its metabolites.
* Other Neurotransmitters levels in serum and brain.
* Pathologic changes in brains;
* Indicators of oxidative stress;

• MOG- induced Experimental Autoimmune Encephalomyelitis (EAE)

Evaluated parameters:
* Disease scoring;
* Evaluation of the spinal cord and brain;
* Detection of blood–brain barrier disruption;
* Detection of neutrophil infiltration;
* Cytokine release;
* Microglial morphology, transformation, and proliferation.

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:

  • ELISA;
  • Colorimetric assay;
  • Histology;
  • Immunohistochemistry;
  • Immunofluorescence;
  • Flow cytometry;
  • Real-time PCR;
  • Western blot;
    *Others (on request).
« Show less
neurology
CNS/Neurology
Neuroscience
neurodegenerative disorders
Alzheimer
Parkinson
amyloid-β- induced alzheimer model
scopolamine-induced amnesia model
streptozotocin-induced model
Rotenone- induced parkinson model
Reserpine- induced parkinson model
6-OHDA- induced parkinson model
MPTP- induced parkinson model
multiple sclerosis
Experimental Autoimmune Encephalomyelitis (EAE)
MOG- induced Experimental Autoimmune Encephalomyelitis (EAE)
Animal Anxiety and Depression Tests
Price on request

There are a number of tests to analyze anxiety and depression-like behavior, CIEnP offers:

Social recognition test
Light-dark box test;
Forced swim test;
Tail suspension test;
Sucrose preference test;
Elevated plus maze;
Open field;
Marble burying test.

There are a number of tests to analyze anxiety and depression-like behavior, CIEnP offers:

Social recognition test
Light-dark box test;
Forced swim test;
Tail suspension test;
Sucrose preference test;
Elevated plus maze;
Open field;
Marble burying test.

« Show less
Mouse
Rat
CNS/Neurology
Neuroscience
anxiety
depression
Social recognition test Light-dark box test
Forced swim test
Tail suspension test
Sucrose preference test
Elevated plus maze
Open field
Marble burying test
neurodegenerative disorders
psychotic
Animal Learning, Memory, and Behavior Tests
Price on request

There are a number of tests to analyze memory and learning processes, CIEnP offers:

Object recognition test
Social recognition test;
Step-down;

There are a number of tests to analyze memory and learning processes, CIEnP offers:

Object recognition test
Social recognition test;
Step-down;

« Show less
Stress & Behavior
neurology
CNS/Neurology
Neuroscience
neurodegenerative diseases
scenescence
Barnes maze
Object recognition test
Social recognition test
Step-down
learning
cognition
Behavior
memory
Nociceptive Pain Animal Models
Price on request

Nociception induced by chemical agents:

  • Formalin-induced nociception;
  • Glutamate-induced nociception
  • Acetic acid-induced nociception
  • Capsaicin-induced nociception
  • Menthol-induced nociception;
  • Cinnamaldehyde-induced nociception
  • Other (upon request);

Surgical incision-induced nociception (post -operative... Show more »

Nociception induced by chemical agents:

  • Formalin-induced nociception;
  • Glutamate-induced nociception
  • Acetic acid-induced nociception
  • Capsaicin-induced nociception
  • Menthol-induced nociception;
  • Cinnamaldehyde-induced nociception
  • Other (upon request);

Surgical incision-induced nociception (post -operative pain);

Hyperalgesia induced by chemical agents:

  • Carrageenan-induced hyperalgesia;
  • PGE2-induced hyperalgesia;
  • Bradykinin-induced hyperalgesia;
  • 5-HT-induced hyperalgesia;
  • Pro-inflammatory cytokines-induced hyperalgesia;
  • Other (upon request);

Hyperalgesia induced by thermal stimulus.

Evaluated parameters
* Number of writhes;
* Licking time;
* Thermal and mechanical hyperalgesic threshold;
* Locomotor activity;
* Plasma extravasation;
* Cell migration (leucocytes);
* Cytokine and other mediators release;
* Enzymes/proteins activities and expression.

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:

  • Colorimetric assay;
  • Flow cytometry;
  • ELISA;
  • Histology;
  • Immunohistochemistry;
  • Immunofluorescence;
  • Real-time PCR;
  • Western blot;
  • Others (on request).
« Show less
Mouse
Rat
Pain
Neuropathic Pain
spinal cord injury
Diabetes
EAE
Neuropathy induced by partial ligation of sciatic nerve
Chemotherapy-induced neuropathy
Neuropathy induced by inflammatory mediators in the brachial plexus
Neuropathy induced by experimental encephalomyelitis (EAE)
Diabetes-induced neuropathy
Neuropathy induced by spinal cord injury after spinal cord compression
neuropathy
Metabolic Disease Animal Models
Price on request

In vitro assays:

  • Cell proliferation in NB2-11 cell line

Experimental models:

-Streptozotocin-induced diabetes

Evaluated parameters:
* Fasting glucose
* Blood glucose levels
* Food intake
* Triglycerides, lipid profiles, lactate dehydrogenase, among others.
* Body weight

- Diet-induced... Show more »

In vitro assays:

  • Cell proliferation in NB2-11 cell line

Experimental models:

-Streptozotocin-induced diabetes

Evaluated parameters:
* Fasting glucose
* Blood glucose levels
* Food intake
* Triglycerides, lipid profiles, lactate dehydrogenase, among others.
* Body weight

- Diet-induced hypertriglyceridemia

Evaluated parameters:
* Fasting glucose
* Blood glucose levels
* Food intake
* Triglycerides, lipid profiles, among others.
* Body weight

- Diet-induced obesity

Evaluated parameters
* Fasting glucose
* Blood glucose levels
* Food intake
* Insulin levels
* Glucose tolerance
* Triglycerides, lipid profiles, among others.
* Body weight

- Hypophysectomy

Evaluated parameters
* Growth hormone levels
* Body growth
* Body weight

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:
* Western blot;
* Real time PCR;
* Colorimetric assays;
* Enzyme assays;
* H&E stain;
* Immunohistochemistry;
* Immunofluorescence;
* Experimental surgery;
* MTT assay;
* Sulphorhodamine B assay;

« Show less
Mouse
Rat
metabolic
Obesity
Diabetes
hypertriglyceridemia
Diet-induced obesity
Hypophysectomy
Diet-induced hypertriglyceridemia
Streptozotocin-induced diabetes
NB2-11 cell line
endocrine
Triglycerides
lipid profiles
glucose
Renal Disease Animal Models
Price on request

In vitro assays:

  • Cell viability in human renal cell (caki-1)

Experimental models:

- Cyclophosphamide-induced haemorrhagic cystitis

Evaluated parameters:
* Non-voiding contractions;
* Bladder pressure;
* Bladder capacity;
* Voided volume;
* Voiding efficiency.

- Isolated organs and... Show more »

In vitro assays:

  • Cell viability in human renal cell (caki-1)

Experimental models:

- Cyclophosphamide-induced haemorrhagic cystitis

Evaluated parameters:
* Non-voiding contractions;
* Bladder pressure;
* Bladder capacity;
* Voided volume;
* Voiding efficiency.

- Isolated organs and tissues

Evaluated parameters:
* Muscle contraction and relaxation

Pharmacodynamics

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques, equipment, methods, and approaches available to be associate to different experimental models, in order to meet the needs of custom assays including:
* Western blot;
* Real time PCR;
* Colorimetric assays;
* Enzyme assays;
* H&E stain;
* Immunohistochemistry;
* Immunofluorescence;
* Experimental surgery;
* MTT assay;
* Sulphorhodamine B assay;

« Show less
Renal
Urinary
organ bath
cystitis
bladder
Cyclophosphamide-induced haemorrhagic cystitis
Isolated organs
renal
bladder
urinary tract
Animal Model in vivo Analyses
Price on request
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Clinical Research
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Immunology Animal Models
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Pharmacology & Toxicology
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Veterinary Research & Diagnostic Services
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Analytical Chemistry Services
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Nephrology Animal Models
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Animal Cognition & Behavior Tests
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Cells and Tissues
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CNS/Neurology Animal Models
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Animal Models of Disease
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Cosmetic Development and Testing
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Xenograft Models
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In vitro Disease Models
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Biology
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Clinical and Anatomic Pathology
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Product Development & Testing
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Pain/Neuropathy Animal Models
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Animal Models and Studies
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Clinical Laboratory Services
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Metabolic Animal Models
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Chemistry and Materials
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Oncology Animal Models
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Gastroenterology Animal Models
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Veterinary Laboratory Services
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