Cicadea Biotech provides custom PCR testing strategies and services for microbe detection and gene detection. Our mission is to improve food safety, animal health, plant health and human health with PCR technology platforms. We also serve as a Contract Research Organization for corporate and government clients. The lab is located at the Helix Center, a Biotech Incubator in St. Louis, Missouri, USA.
We provide high-throughput screening for CRISPR transformed cell lines and plants.
We have a QuantStudio 5 Realtime PCR with 384-well format. With this REaltime PCR machine, we can perform high-throughput PCR screening services. We offer high-throughput testing to screen genetic markers.
Why PCR? Traditional culture methods have been commonly used for microbial detection and microbial identification, however, they have limitations. Due to a lengthy culture time (1-7 days) and significant challenges for culturing some microorganisms, culture methods cannot provide rapid diagnostic solutions. Polymerase Chain Reaction (PCR) has demonstrated its effectiveness to detect and identify microbes in less than a few hours with precision.
What are our target microbes? Our assays target a broad spectrum of microbes including bacterial and viral pathogens that infect plants, animals, and humans. We want to help our clients to confirm microbial presence in products and to detect microbial contamination in environmental, agricultural, or clinical samples. Our PCR-based assays can be tailored to meet clients’ specific needs.
PCR technology has been widely used for gene detection by scientists. Cicadea Biotech serves as a contract research organization to perform PCR testing for gene detection. We work with clients to develop custom PCR assays for gene detection and perform high-throughput PCR testing. We provide rapid-testing solutions to detect transgenes in plants and human cancer-related genes. We also perform genomic analysis on any DNA samples.
Our turn-around time for PCR testing can be from 24 hours to days depending on the availability of the assays. The turn-around time for new assay development varies by the availability of materials and assay complexity. We work closely with clients to come up with a reasonable timeline for both assay work and testing work.
With our expertise in assay development of Taqman PCR (or probe-based PCR) assays, we can provide the best target specific qPCR assays for clients. Our assays are tested by standard QC tests to ensure assay quality and to meet clients' needs.
With our expertise in copy number analysis via Taqman qPCR assays, we can help clients to perform gene copy number analysis by developing customized assays and by performing PCR testing. The turn around time can be reasonable to meet clients' needs.
Our expertise in developing Taqman PCR assays can be applied to complete projects related to Taqman assays. We would like to work closely with clients to meet their testing needs.
With our expertise in assay development of PCR assays, we can work with clients to develop customized pathogen detection assay panels for testing purposes. We can provide viable PCR assays to screen many samples for pathogen detection. Our currently developed assays are E. coli O157 and Salmonella.
Run digital PCR assays for cancer detection or other purposes
With the new Droplet Digital PCR (ddPCR) technology, we can detect cancer mutations and pathogens with high sensitivity and accuracy. We provide volume discount for large sample volume.
Cicadea Biotech focus on microbial detection with PCR technologies. We have worked with human pathogens in the past and we can perform PCR amplification test to screen for infectious microbes, such as bacteria, fungus and viruses.
With our experience in genomic DNA extraction from plant samples and bacterial samples, we can work closely with clients to meet their testing needs. Price varies by different sample sizes. Volume discount may be offered.
We can provide PCR assays to identify specific microorganisms and also perform strain typing. We work closely with clients to meet their testing needs.
We perform qPCR for RT-PCR assays. We can develop probe-based PCR assays and run PCR assays to identify the target specific RNA from samples. We work closely with clients to meet their needs.
Cicadea Biotech develops and perform multiplex PCR assays. We work closely with clients to ensure assay meet the clients' quality standards.
We have a QuantStudio 5 Realtime PCR machine with 384-well format. We can Perform large scale genomic DNA analysis on bacteria or others. With our industrial experience in high throughput DNA analysis, we can perform large scale genomic DNA analysis on bacteria from any samples.
Develop custom PCR assays to detect microbes and genes
We can develop PCR assays according to the known DNA sequence. Our assays will be customized to meet your needs. They are validated according to standard QC protocols. We can design multiplex PCR assays to ensure quality assay performance.
We can provide DNA-based testing services with PCR technology platforms.
With many years of biotech industry experience, we can work with clients to develop PCR-based genotyping assays and provide PCR-based genotyping services for plants or other samples. We can have fast turnaround time when all resources are available.
Our expertise in qPCR testing can help clients to perform both qualitative analysis and quantitative analysis.
With many years of assay design experience, we can help clients to design PCR assays. We can also run quality control tests to ensure the quality assay design.
We can develop RT-PCR assays and run RT-PCR assays. We work closely with clients to meet their needs.
With many years of biotech industry experience, we can work with clients to develop PCR assays to detect transgenes and also provide PCR testing for the transgenic plants. We can provide various types of PCR assays including event specific assays, gene specific assays, event specific zygosity assays. Price varies depending on the assay type and sample sizes. We can work with clients to develop a most efficient testing strategy.
With our expertise in large volume or high-throughput DNA extraction, we can provide DNA extraction services to isolate DNA from plants, bacteria and other samples.
Biochemistry & Molecular Biology Services
We can develop PCR assays for gene detection and microbial detection. PCR assays can be probe-based or Taqman PCR. These PCR assays can be realtime PCR assays.
Twenty years of industrial genetic engineering experience. We would like to offer high-throughput gene detection for genetic engineering plants or cells.
"We needed to read ddPCR plates. We sent the plates and received results the following day. They were quick to reply to any questions we had."