ChiroSolve

Price: Depends on the number chiral centers, number of enantiomers, molecule type, and the amount needed
Duration: 7 to 15 days

EnantioPrep or method development service offers quick delivery of enantiopure compounds for medicinal chemistry needs within matter of days and defines a stable method that can be used for scale-up production of enantiomers to kilo quantities.
How it works: After the best 3 chiral separation conditions are determined using “Chiral Screen” service, we do bench-top scaleup using each of the 3 resolutions conditions to determine which separation condition offers maximum yield and purity using minimum re-crystallization steps.
If needed, first we recover the racemate from screening kits, prepare the diastereomeric salt using selected chiral resolving agent and solvent; and then purify the salt using series of re-crystallization steps until target purity is achieved. Each step involves treating the increasingly purified enantiomer mixture with the selected solvent; heating and cooling the mixture; and extracting the further purified enantiomeric crystals.
If both enantiomers are needed, the second enantiomer is recovered from the initial filtrate and above enrichment steps are performed using the selected resolution condition for that enantiomer.

Note: All our services are considered “research projects” and we cannot guarantee that the enantiomeric purity will be achieved. Work is done under best-effort basis.
Results

• Up to 90% of the racemate used during screening can be recovered; maximizing the use of racemate to deliver pure enantiomer
• Pure enantiomer obtained after repeated re-crystallization may yield up to 1/3rd quantity of the racemate used
• Up to 5 gm. of pure enantiomer and detailed step-by-step separation method can be developed within matter of days

Key Benefits

• Quick turn-around time to receive pure enantiomer using consistent and robust technology
• Gives "Peace of mind" knowing that the method being defined can be easily scale-up and optimized
• No additional set-up, or investment of expensive instrument needed if larger quantities of enantiomer are needed

ChiroSolve offers “one stop shop” experience towards outsourced lead discovery and optimization efforts. We can deliver the compounds from milligrams to 50 gm quantity in-house; and can work with our partners to scale-up beyond this quantity. We can deliver:

• Organic synthesis: chiral, sugars. Amino acids, peptides, analogs, metabolites, building blocks
• API synthesis: fluorescent probes, medicinal chemistry scaffolds, structure-activity relationship
• Reference Compounds
• Intermediates And Derivatives Of Lead Compounds
• Isotopically Labeled And Unlabeled Compounds

Note: All our services are considered “research projects” and we cannot guarantee that the enantiomeric purity will be achieved. Work is done under best-effort basis.

Customer Centric Services

Medicinal Chemistry Services

• Lead optimization and structure-activity relationship profiling
• Rapid derivatisation of small molecules
• Synthetic feasibility study

Process Development

• Small-Scale Synthesis of API and Intermediates
• Method development and optimization

R&D Services

• Custom synthesis of chiral and non-chiral building blocks
• Parallel synthesis of focused libraries (lead generation)
• Synthesis of metabolites and impurities

Price: $5,000 to $8,000; Duration: 3 to 5 days
Chiral Screen: We screen the given racemate against more than 384 chiral separation conditions to effectively identify all commercially viable conditions for both +ve and -ve enantiomers. Using our high-throughput screening kits with patent-pending technology, we can provide the results within 3 to 5 days.
How it works: Each 96-vial kit contains unique combination of pre-calibrated amount of chiral resolving agent and polar solvent. We use 4 different such kits (comprising of 384 unique conditions). During the experiment racemate is added to each vial of the kit with 1:1 ratio with the resolving agent. The kits are then heated together in water bath to up to 800C and then cooled to ambient temperature for 24 hours. The vials with crystals then show +ve results and need to be analyzed further to check if any enantiomeric enrichment was achieved. Analysis is done using Digital Polarimeter (to read +ve or –ve specific rotation) and / or through chiral HPLC.
Results: Delivery includes:
• Results Charts that show the amount of crystals and the specific rotation for the vials which yielded the +ve results. Based on the amount of yield and specific rotation, we also identify the best 3 resolution conditions.
• Vials with +ve results (both crystals and filtrate) so the customer can validate the results
**Note: All our services are considered “research projects” since the results are dependant on many factors including the type of target racemate. As such we cannot guarantee that target enantiomeric purity will be achieved. Work is done on a best-effort basis.

Key Benefits
• Confidence that you have considered all commercially viable conditions for the chiral resolution of your racemate. This allows you to establish IP foundation fully
• Provides number of possibilities for rapid preparation of enantiomer for early lead identification and preclinical testing