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CGIBD Advanced Analytics Core

100% Positive
18 Orders Completed
Chapel Hill, North Carolina, US

About CGIBD Advanced Analytics Core

The CGIBD's Advanced Analytics (AA) Core provides high-quality, quantitative solutions for studying proteins, peptides, and other molecules, including mRNA and cDNA. The Core's immunotechnologies (IT) component provides consultation, ordering, and immunoassay services, and supports a wide variety of... Show more »

The CGIBD's Advanced Analytics (AA) Core provides high-quality, quantitative solutions for studying proteins, peptides, and other molecules, including mRNA and cDNA. The Core's immunotechnologies (IT) component provides consultation, ordering, and immunoassay services, and supports a wide variety of analytes in both traditional ELISA/EIA and xMAP-based multiplex bead assay formats.

The AA Core also offers high-throughput real-time qPCR service on its Fluidigm BioMark HD system, allowing users to simultaneously measure as many as 96 samples for up to 96 genes, and requiring as little as a single cell per sample when used in combination with the Core's C1 single-cell auto prep system.

Whether measuring cytokines or gene expression, the AA core's favorable pricing and unique cost-sharing model allow investigators access to a wide variety of assays and an extensive probe library for a fraction of their list price.

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Our Services (7)


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Single Cell RNA-Seq

Starting at $3,110.00 per sample

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Platforms: 10x Genomics, Fluidigm C1
Services offered: Library Preparation, Sequencing, Basic Data Processing

10X Single Cell 3' or 5' Library Preparation
1st sample: ~$3,900 per sample
Samples 2-8: ~$3,110 per sample

Pricing for other 10x services (5' VDJJ,... Show more »

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Platforms: 10x Genomics, Fluidigm C1
Services offered: Library Preparation, Sequencing, Basic Data Processing

10X Single Cell 3' or 5' Library Preparation
1st sample: ~$3,900 per sample
Samples 2-8: ~$3,110 per sample

Pricing for other 10x services (5' VDJJ, ATAC-Seq, etc...) is available upon request.

Fludigm C1 RNA-Seq
Standard (96 cells): ~$4,980
HT (800 cells): ~$5,450

NextSeq-500 Sequencing & Basic Data Analysis
Mid-output 150 cycles: ~$2,200
Mid-output 300 cycles: ~$3,500
High-output 150 cycles: ~$5,640
High-output 300 cycles: ~$8,710

Mid-output yields ~130M read pairs
High-output yields ~400M read pairs

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Fluidigm C1 Illumina NextSeq 500 Bio-Rad ddSeq 10x Genomics Chromium System

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Single Cell Isolation

Starting at $2,340.00 per run

Note: UNC/NCSU/CGIBD Members receive a ~35% discount
Fluidigm C1

The C1 system isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers... Show more »

Note: UNC/NCSU/CGIBD Members receive a ~35% discount
Fluidigm C1

The C1 system isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers or reporter genes. After staining, cells are automatically lysed and template is quickly prepared for qPCR or sequencing analysis—all in as little as four hours.

C1 STA (up to 96 cells): ~$2,340
C1 RNASeq (up to 96 cells): ~$4,980
C1 HT RNASeq (up to 800 cells): ~$5450
C1 REAP-Seq or DNA-Seq: Pricing available upon request

The AA Core offers cell sorting services to assist C1 customers with sample preparation. Alternatively, we accept single cell suspensions in 1X PBS with 0.1% BSA. Optimal cell concentration depends on the IFC being used. Please consult with the core manager to ensure appropriate sample specifications.

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Fluidigm C1

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PCR

Starting at $2,645.00 per run

Note: UNC/NCSU/CGIBD Members receive a ~35% discount
Fluidigm BioMark HD

High-throughput real-time qPCR using a Fluidigm BioMark HD system, allowing simultaneous assay of up to 96 samples for as many as 96 genes.

48.48: ~$2,645
96.96: ~$4,675
192.24: ~$3,900

Samples should be submitted in a conical bottom PCR plate... Show more »

Note: UNC/NCSU/CGIBD Members receive a ~35% discount
Fluidigm BioMark HD

High-throughput real-time qPCR using a Fluidigm BioMark HD system, allowing simultaneous assay of up to 96 samples for as many as 96 genes.

48.48: ~$2,645
96.96: ~$4,675
192.24: ~$3,900

Samples should be submitted in a conical bottom PCR plate following a standard assay design layout and accompanied by a template that outlines the sample order and genes to be tested..

Please provide exactly 5 uL of mRNA or cDNA and note it in the sample template. C1 customers can skip this step, as they will be providing cells rather than nucleic acids. Prospective users are advised to contact the core manager to ensure that someone will be available to receive their samples and the accompanying template when they are delivered.

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Fluidigm Biomark HD qPCR RT-qPCR qRT-PCR mutiplex qPCR Fluidigm Show 6 more tags Show less

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Fluorescence Activated Cell Sorting (FACS)

Fluorescence Activated Cell Sorting
Starting at $311.00 per run

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Sony SH800 FACS
Only available in conjunction with genomics services

First hour: $311
Additional FACS time: +$155.52/hr

The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes (70-130 μm) and supports sorting into tubes and 96- and 384-well... Show more »

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Sony SH800 FACS
Only available in conjunction with genomics services

First hour: $311
Additional FACS time: +$155.52/hr

The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes (70-130 μm) and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. The SH800S is for non-clinical research use only and is a Class 1 laser product.

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Sony FACS cell sorter SH800S Flow cytometry FACS

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ELISA

Enzyme-linked immunosorbent assay
Starting at $205.00 per plate

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Assays are performed using repeating electronic multipipettes (VistaLabs Ovation), washed using a Bio-Tek ELx405rs 96-well plate washer, and read on a Bio-Tek Synergy HT microplate spectrophotometer.

  • Consultation: $80 per hour beyond the first
  • Assay materials: varies... Show more »

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Assays are performed using repeating electronic multipipettes (VistaLabs Ovation), washed using a Bio-Tek ELx405rs 96-well plate washer, and read on a Bio-Tek Synergy HT microplate spectrophotometer.

  • Consultation: $80 per hour beyond the first
  • Assay materials: varies based on assays requested and current pricing
  • Assay service/Labor: $205 per plate
  • Additional processing/handling (plating, etc): $3.15 per sample
  • Absorbance/Fluorescence/Luminescence reading: $16 per plate

All immunoassay samples should be submitted in ready-to-assay form. Any pre-extraction or sample preparation procedures are the sole responsibility of the user, unless alternate arrangements have been made in advance with the core manager.

Samples should be submitted in a minimum-binding 96-well plate (we recommend Fisher part number 12-565-505) following a standard assay design layout and accompanied by a template that outlines the sample order.

Please provide sufficient volume for all samples to be assayed in duplicate, plus an additional 25-50 uL. Samples to be diluted may be submitted at the exact volume required to dilute them up to 250-300uL evenly. Prospective users are advised to contact the core manager to ensure that someone will be available to receive their samples and the accompanying template when they are delivered.

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BioTek microplate washer BioTek Synergy HT ELISA

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Biomarker Analysis

Starting at $235.00 per plate

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Bead-based multiplex assays allow the simultaneous assay of up to 100 different analytes per sample in a 96-well plate using as little as 10 uL per sample. Assays are performed using repeating electronic multipipettes (VistaLabs Ovation), washed using a handheld magnetic... Show more »

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

Bead-based multiplex assays allow the simultaneous assay of up to 100 different analytes per sample in a 96-well plate using as little as 10 uL per sample. Assays are performed using repeating electronic multipipettes (VistaLabs Ovation), washed using a handheld magnetic separator block (Millipore), and read on a Bio-Plex 200 suspension array system with high-throughput fluidics.

  • Consultation: $80/hr (after first)
  • Assay Materials: Varies by assay & vendor
  • Processing/Handling: $3.15/sample
  • Multiplex Assay Service: $235/plate
  • w/ DropArray Miniaturization: +$1,245/plate
  • Multiplex Array Reading: $50/plate

All multiplex samples should be submitted in ready-to-assay form. Any pre-extraction or sample preparation procedures are the sole responsibility of the user, unless alternate arrangements have been made in advance with the core manager.

Samples should be submitted in a minimum-binding 96-well plate (we recommend Fisher part number 12-565-505) following a standard assay design layout and accompanied by a template that outlines the sample order.

Please provide sufficient volume for all samples to be assayed in duplicate, plus an additional 15-30 uL. Samples to be diluted may be submitted at the exact volume required to dilute them up to 75-100 uL evenly.. Prospective users are advised to contact the core manager to ensure that someone will be available to receive their samples and the accompanying template when they are delivered.

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Multiplex immunoassays Bio-Plex Luminex®

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Single Cell Western Blot

Starting at $700.00 per run

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

ProteinSimple Milo

The Milo single-cell Western blot platform measures the expression of multiple proteins across 1,000+ cells in just 4-6 hours. Simply provide your single-cell suspension and compatible primary antibodies and the AA Core will return fluorescence images.... Show more »

Note: UNC/NCSU/CGIBD Members receive a ~35% discount

ProteinSimple Milo

The Milo single-cell Western blot platform measures the expression of multiple proteins across 1,000+ cells in just 4-6 hours. Simply provide your single-cell suspension and compatible primary antibodies and the AA Core will return fluorescence images. Protein expression can then be quantified using Protein Simple's free Scout Software. Milo is a powerful tool both alone and in combination with the Core’s other single cell genomics services (Fluidigm BioMark HD and Fluidigm C1).

Applications of the Milo system include:

  • single cell RNA-Seq validation
  • Cell subtype, protein isoform, and phospho-protein heterogeneity identification
  • Histone modification detection
  • Gene editing efficiency determination
  • Protein expression in low-abundance samples
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western blot quantitative western blot Single cell

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Carlton Anderson

Core Manager & Associate Director
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Gabrielle Cannon

Research Specialist
  • Positive review for Luminex Multiplex Assays:

    July 2014

    "Good quality of data using low amount of sample volume at very fair place."

CGIBD Advanced Analytics Core has not received any endorsements.