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Centro Nacional de Biotecnologia CNB

Madrid, ES

The National Centre for Biotechnolgy (CNB) is a research centre that forms part of the Spanish National Research Council (Consejo Superior de Investigaciones Científicas, CSIC), Spain’s most important public scientific institution. The CNB opened in 1992 to lead in developing modern biotechnology in Spain. Our principal objectives are to:

  • Acquire knowledge and develop new technologies in the areas of human and animal health, agriculture and the environment.
  • Promote knowledge and technology transfer for the benefit of society.
  • Train future generations of researchers and technologists.
  • Engage and inform society about advances and the benefits of biotechnology.

The CNB is distinguished by its versatile interdisciplinary research that combines molecular biology methods with the latest technology in the fields of functional and structural biology. We are a team of more than 600 professionals committed to... Show more »

The National Centre for Biotechnolgy (CNB) is a research centre that forms part of the Spanish National Research Council (Consejo Superior de Investigaciones Científicas, CSIC), Spain’s most important public scientific institution. The CNB opened in 1992 to lead in developing modern biotechnology in Spain. Our principal objectives are to:

  • Acquire knowledge and develop new technologies in the areas of human and animal health, agriculture and the environment.
  • Promote knowledge and technology transfer for the benefit of society.
  • Train future generations of researchers and technologists.
  • Engage and inform society about advances and the benefits of biotechnology.

The CNB is distinguished by its versatile interdisciplinary research that combines molecular biology methods with the latest technology in the fields of functional and structural biology. We are a team of more than 600 professionals committed to research excellence. The CNB has been recognised by an international jury as one of the eight Severo Ochoa Centres of Excellence in medicine and the life sciences.

Selected Publications

  • Eva M. García-Cuesta, Gloria Esteso, Omodele Ashiru, Sheila López-Cobo, Mario Álvarez-Maestro, Ana Linares, Mei M. Ho, Luis Martínez-Piñeiro, Hugh T. Reyburn & Mar Valés-Gómez. Characterization of a human anti-tumoral NK cell population expanded after BCG treatment of leukocytes. Oncoimmunology 2017, e1293212e1293212
  • Muñoz A, Mangano S, González-García MP, Contreras R, Sauer MB, De Rybel B, Weijers D, Sánchez-Serrano JJ, Sanmartín M, Rojo E. Rima-dependent nuclear accumulation of iyo triggers auxin-irreversible cell differentiation in arabidopsis. Plant Cell 2017 Feb 21. pii: tpc.00791.2016. doi: 10.1105/tpc.16.00791
  • Morales L, Oliveros JC, Fernandez-Delgado R, tenOever BR, Enjuanes L, Sola I. SARS-CoV-encoded small RNAs contribute to infection-associated lung pathology. Cell Host Microbe. 2017 Feb 12. pii: S1931-3128(17)30037-9. doi: 10.1016/j.chom.2017.01.015
  • Francis Impens, Nathalie Rolhion, Lilliana Radoshevich, Christophe Bécavin, Mélodie Duval, JeffreyMellin, Francisco García del Portillo, M. Graciela Pucciarelli, Allison H. Williams and Pascale Cossart. N-terminomics identifies PRLI42 as a membrane miniprotein conserved in firmicutes and critical stressome activation in listeria monocytogenes. Nature Microbiology 2017; 2: 17005 (2017), doi:10.1038/nmicrobiol.2017.5doi:10.1038/nmicrobiol.2017.5
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X-ray crystallography
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Protein X-ray crystallography is a high-resolution technique that allows us to study protein structure at atomic level. This method provides a detailed view of protein function, ligand and protein interactions, supramolecular organisation and mutants related to human diseases. Great improvements both in crystallisation techniques... Show more »

Protein X-ray crystallography is a high-resolution technique that allows us to study protein structure at atomic level. This method provides a detailed view of protein function, ligand and protein interactions, supramolecular organisation and mutants related to human diseases. Great improvements both in crystallisation techniques and software for structure resolution and refinement have been achieved since the last decade, increasing the chances of solving a macromolecule structure

The macromolecular X-ray crystallography facility provides the following techniques:

Advice and supervision of protein production from cloning to expression in bacterial, yeast and eukaryotic systemsSupport and training in protein purification to obtain crystal-grade proteinAutomated macromolecular crystallisationOptimisation of crystallisation conditions applying standard and in-house techniquesCrystal mounting, access to synchrotron beam time, X-ray diffraction data collectionData processing and structure resolution and analysis

Service equipment:

Three temperature-controlled crystallisation roomsGenesis RSP 150 workstation (Tecan Trading AG) nanodispenser robotRigaku Desktop Minstrel system for automated visualisation of crystallisation platesCrystalTrak database suite for crystallisation screening and improvement of positive trials.

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Confocal Microscopy
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Confocal microscopy imaging techniques use lasers and electronic systems of digital image capture to provide optical sections of the material. The presence of fluorescent markers in the sample allows location of cell components in single sections and various experimental approaches involving single or multiple fluorescent... Show more »

Confocal microscopy imaging techniques use lasers and electronic systems of digital image capture to provide optical sections of the material. The presence of fluorescent markers in the sample allows location of cell components in single sections and various experimental approaches involving single or multiple fluorescent labelling in fixed cells and tissues.

The service provides infrastructure for widefield, confocal laser scanning and STED super-resolution microscopy, covering most light microscopy applications with technical assistance to all its users. The equipment and services are available to all CNB personnel as well as to researchers from the public and private sectors. The technical staff offers training and advice about equipment use, available methods, and image processing, quantification and analysis if required. Cell culture slides, aliquots of secondary antibodies and probes with broad use in fluorescence microscopy applications are also provided.

The facility’s equipment includes:

  • Confocal multispectral Leica TCS SP5 system. Laser lines: 405, 458, 476, 488, 514, 561, 594 and 633 nm. Incubation system for time-lapse assays.
  • Confocal multispectral Leica TCS SP8 system with a 3X STED module for super- resolution. Laser lines: 405 nm and WLL2 (white laser for 470-670 nm excitation). Depletion lines: 592 and 660 nm.
  • Fluorescence microscope Leica DMI6000B with incubation system for time-lapse studies and OrcaR2 monochrome digital camera for image detection.
  • The unit also provides offline computer workstations for fluorescence and confocal image processing and analysis (LAS AF, LAS X, MetaMorph, Fiji/ImageJ, Huygens, Imaris, Cell Profiler).
  • Auxiliary equipment: CO2 incubator, centrifuge, laminar flow chamber, freezer.

Laser scanning confocal microscopy applications:

  • Multichannel confocal imaging + transmission imaging of living cells or fixed samples (2D, 3D, 4D imaging).
  • High speed confocal microscopy.
  • STED 3X super-resolution.
  • Multidimensional in vivo time-lapse experiments
  • FRET, FRAP, photoactivation, photoswitching, excitation/emission lambda scan, calcium imaging.
  • Subcellular colocalization studies.

Widefield applications:

  • Multichannel fluorescence imaging + transmission imaging (BF, DIC, phase contrast)
  • Multidimensional in vivo time lapse experiments (wound healing, infection, etc.)
  • Tile scan imaging
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Electron Microscopy
Price on request

The electron microscopy service offers a variety of equipment and techniques for the preparation, processing and analysis of biological samples (cell and bacterial cultures, cell fractions, proteins, viruses, animal and plant tissues) by transmission electron microscopy.

The technical staff provides support to users in the... Show more »

The electron microscopy service offers a variety of equipment and techniques for the preparation, processing and analysis of biological samples (cell and bacterial cultures, cell fractions, proteins, viruses, animal and plant tissues) by transmission electron microscopy.

The technical staff provides support to users in the correct use of equipment and methodologies. We offer regular training in the techniques and methods available. We also take care of the sample preparation, if required, and image acquisition and provide support for data interpretation.

Techniques offered include chemical fixation and inclusion in epoxy and acrylic resins, cryofixation (plunge freezing, high pressure freezing), freeze substitution and inclusion in low temperature resins, ultramicrotomy, negative staining, immunonegative staining, immunolabelling, in situ hybridisation, conventional transmission electron and low dose electron microscopy.

Specialised equipment

  • JEOL JEM 1011 transmission electron microscope with Gatan ES1000Ww camera
  • Leica Ultracut UC6 cryo-ultramicrotome
  • Reichert Ultracut E ultramicrotome
  • Automatic cryosubstitution system Leica EM AFS2
  • High-pressure vitrification system Leica EM PACT2
  • Sample trimmer Leica EM TRIM
  • Knifemaker Reichert
  • Carbon coating system Leica EM MED020
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Optical Microscopy
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Microscopy
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Biology
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Fluorescence-Based Microscopy
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Imaging & Spectroscopy
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Biochemistry & Molecular Biology
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Structural Biology
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2017-12-21 04:13:05 -1000

Net Promoter Score of 9 received for X-ray crystallography.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.

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