1349747275000

Center of Excellence in Nutrigenomics

State College, Pennsylvania, US

The Center of Excellence in Nutrigenomics (CEN) is striving to be the focal point of molecular nutrition studies. The Center is a conduit to the Huck Institute of the Life Sciences and their genomics initiatives as well as the General Clinical Research Center and their expertise in human nutrition studies. Thus, PSU Nutrigenomics can fulfill our goal to understand nutrition "from the table to the gene and back again".

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Affymetrix RNA microarray
Starting at $200.00 per sample

Processing of Affymetrix slides (includes hybridization, washing, scanning)

Processing of Affymetrix slides (includes hybridization, washing, scanning)

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Assay Development
Price on request
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Apoptosis Assays
Starting at $4.00 per sample

Measure caspase 3/7 using a luminescent assay.

Measure caspase 3/7 using a luminescent assay.

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Cholesterol Efflux Assay
Starting at $50.00 per sample

The process of removing excess cholesterol from the periphery for transport and removal at the liver (reverse cholesterol transport, RCT) is important in preventing atherosclerosis. The first step is cholesterol efflux from macrophages. In these assays we can examine the ability of drugs and compounds to affect this process or... Show more »

The process of removing excess cholesterol from the periphery for transport and removal at the liver (reverse cholesterol transport, RCT) is important in preventing atherosclerosis. The first step is cholesterol efflux from macrophages. In these assays we can examine the ability of drugs and compounds to affect this process or alternatively measure the ability of serum samples to provide carriers proteins. We have developed a non-radioactive methods that is amenable to high-throughput screening ( Zhang J., S. Cai, B. R. Peterson, P. M. Kris-Etherton, and J. P. V. Heuvel. 2011. Development of a cell-based, high-throughput screening assay for cholesterol efflux using a fluorescent mimic of cholesterol. Assay Drug Dev Technol 9:136-146.)

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Cryopreservation
Starting at $280.00 per sample

Mammalian cells will be transiently transfected with the necessary plasmids for performing nuclear receptor reporter assays. The cells will be frozen using Indigo's proprietary Cryomite technology. Quality control will be performed and provided to the client prior to shipping. Detailed instructions for the thawing and... Show more »

Mammalian cells will be transiently transfected with the necessary plasmids for performing nuclear receptor reporter assays. The cells will be frozen using Indigo's proprietary Cryomite technology. Quality control will be performed and provided to the client prior to shipping. Detailed instructions for the thawing and treatment of the assay will be provided. Available assays can be found at indigobiosciences.com

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Genomic DNA Extraction
Starting at $25.00 per sample

DNA is isolated, quantified and diluted

DNA is isolated, quantified and diluted

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Genomics Consulting
Starting at $250.00 per hour
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Mammalian Cell Culture
Starting at $800.00 per sample

Cell culture setup. Includes cell line propagation, transfection and treatment. Experiments are performed in 96-well plates with a maximum of 10-plates per day. We can grow many different transformed cell lines, please inquire about availability.

Cell culture setup. Includes cell line propagation, transfection and treatment. Experiments are performed in 96-well plates with a maximum of 10-plates per day. We can grow many different transformed cell lines, please inquire about availability.

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Microarray Data Analysis
Starting at $200.00 per hour

Analysis, consulting and data interpretation

Analysis, consulting and data interpretation

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Plasmid DNA Preparation
Price on request

DNA is isolated, quantified and diluted

DNA is isolated, quantified and diluted

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RNA Extraction
Starting at $24.00 per sample

RNA extraction appropriate for real-time PCR

RNA extraction appropriate for real-time PCR

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qPCR
Quantitative PCR
Price on request

Real time PCR will be performed using SYBR green as the indicator in an ABI 7300 detection system.

Real time PCR will be performed using SYBR green as the indicator in an ABI 7300 detection system.

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DNA Extraction and Purification
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Cell-Based Assays
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Nucleic Acid Analysis
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Biology
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DNA
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PCR
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Nucleic Acid Services
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Cellular Health & Metabolism Assays
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RNA Microarray
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Nucleic Acid Data Analysis
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Biospecimens
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Cryostorage
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Experimental Design
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Cells and Tissues
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Project Management, Consulting, & Support Services
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Biospecimen Management and Storage
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Cell Death Assays
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Biochemistry & Molecular Biology
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RNA
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Plasmids and Vectors
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RNA Microarray Platforms
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