1381777811414 3856cab1396a93a0e15896df1e0d7aeb

Center for Bioinformatics & Functional Genomics

Oxford, Ohio, US

The CBFG is the onsite core molecular biology and bioinformatics resource at Miami University (Oxford, OH). We are focused mainly on DNA and protein analysis, but also provide expertise in robotics liquid handling.

Training in the CBFG supports a number of important programs at Miami University. These include the interdisciplinary Cell, Molecular, and Structural Biology (CMSB) program and the Undergraduate Research and Mentoring (URM) program funded by the National Science Foundation.

Center for Bioinformatics & Functional Genomics has not listed any services.

Bioinformatics
Starting at $350.00 per unit

We will perform basic bioinformatics analysis for some projects on a case-by-case basis. The scope and price will be evaluated on a per-project basis. Example: 16S amplicon project would be $350.

We will perform basic bioinformatics analysis for some projects on a case-by-case basis. The scope and price will be evaluated on a per-project basis. Example: 16S amplicon project would be $350.

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16S rRNA Sequencing
Starting at $20.00 per sample

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the V4 (515f/806r) region. Currently, we use the EMP primer design (old design, not the new). Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing... Show more »

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the V4 (515f/806r) region. Currently, we use the EMP primer design (old design, not the new). Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis unless added separately; but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.

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18S rRNA Sequencing
Starting at $20.00 per sample

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the SSU (1391f/Br) region. Currently, we use the EMP primer design. Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing (minimum of about $2500 per... Show more »

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the SSU (1391f/Br) region. Currently, we use the EMP primer design. Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis, but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.

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ITS Sequencing
Internal Transcribed Spacer Sequencing
Starting at $20.00 per sample

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the ITS region. Currently, we use the primer design from (Smith, D. P. & Peay, K. G. Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing. PLoS One 9, e90234 (2014)).... Show more »

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the ITS region. Currently, we use the primer design from (Smith, D. P. & Peay, K. G. Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing. PLoS One 9, e90234 (2014)). Contact for pricing - usually between $20 to $30 per sample - depending on the number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis unless added separately, but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.

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Illumina Sequencing
Starting at $1,999.00 per lane

MiSeq Sequencing Only. We can perform most next generation sequencing protocols suitable for the MiSeq platform. These would include dual INDEX Nextera amplicon sequencing (including 12 bp GOLAY INDEX 16S,18S and ITS emp Protocol), microbial genome sequencing, bacterial metagenomics, RNA-Seq (limited scope). Generally, we run... Show more »

MiSeq Sequencing Only. We can perform most next generation sequencing protocols suitable for the MiSeq platform. These would include dual INDEX Nextera amplicon sequencing (including 12 bp GOLAY INDEX 16S,18S and ITS emp Protocol), microbial genome sequencing, bacterial metagenomics, RNA-Seq (limited scope). Generally, we run and load "ready-to-go" libraries, but will consider library construction on a case by case basis. Please contact us directly to discuss total project costs.

For a 2x300 v2 MiSeq = $1250
For a 2x50 v2 MiSeq = $950
For a 2x500 v2 MiSeq = $1400

For v3 kits please contact (Illumina pricing is in flux).

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DNA Sequencing
Starting at $3.00 per sample

We run analysis of BigDye 3.1 Terminator Cycle Sequencing (Sanger) on both ABI 3130xl and ABI 3730 Capillary Electrophoresis instruments. We can also analyze GeneMapper with Dye Set D and G5. Data is provided as .ab1, .phd.1 (Sanger) or .fsa (GM) files.
Individual samples are accepted and analyzed in groups of 16, or 48. Full... Show more »

We run analysis of BigDye 3.1 Terminator Cycle Sequencing (Sanger) on both ABI 3130xl and ABI 3730 Capillary Electrophoresis instruments. We can also analyze GeneMapper with Dye Set D and G5. Data is provided as .ab1, .phd.1 (Sanger) or .fsa (GM) files.
Individual samples are accepted and analyzed in groups of 16, or 48. Full plate (96 samples) are discounted at $213. No minimum numbers of samples are required. We accept the samples as post-run cleaned & dried down. Please see our recommend protocol (this site, or contact Dr. Kiss). We will add Hi-Di Formamide (20 microL) and perform the CE analysis.

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DNA Fragmentation Assays
Starting at $25.00 per sample

Fragmentation of DNA for downstream use in NGS based protocols. Shearing will be done on a Covaris M220 Sonicator. We highly recommend combining shearing with a QC check on a HS DNA Chip (Bioanalyzer2100).

Fragmentation of DNA for downstream use in NGS based protocols. Shearing will be done on a Covaris M220 Sonicator. We highly recommend combining shearing with a QC check on a HS DNA Chip (Bioanalyzer2100).

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DNA Fragment Analysis
Starting at $75.00 per chip

High Sensitivity DNA Chip Kit Analysis by Bioanalyzer2100. Samples can be sized from 50 to 7000 kbps, 11 samples per chip (gel), quantitative range (concentration) is 5-500 pg/μl.

High Sensitivity DNA Chip Kit Analysis by Bioanalyzer2100. Samples can be sized from 50 to 7000 kbps, 11 samples per chip (gel), quantitative range (concentration) is 5-500 pg/μl.

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RNA Quality Control
Starting at $40.00 per chip

Analysis of total RNA by RNA Pico 6000 Bioanalyzer2100. Eleven samples can be done on a single chip. Please ensure samples are in the range of 250–5000 pg/μl - minimum of 5 μl is required for analysis.

Analysis of total RNA by RNA Pico 6000 Bioanalyzer2100. Eleven samples can be done on a single chip. Please ensure samples are in the range of 250–5000 pg/μl - minimum of 5 μl is required for analysis.

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Robotics Engineering
Starting at $250.00 per run

Custom Robotics Protocols on a Beckman-Coulter BiomekFX Liquid Handling Robot. Dual Pod (AP96 and SPAN8), orbital shaker ALP, SPE vacuum ALP, chilling/hating ALPS, Tip Washer ALP, Promega MagnaBot (II) ALP accessory, Bar Code Reader ALP.

Initial setup for a new, not previously programmed project is $500 + consumables. For a... Show more »

Custom Robotics Protocols on a Beckman-Coulter BiomekFX Liquid Handling Robot. Dual Pod (AP96 and SPAN8), orbital shaker ALP, SPE vacuum ALP, chilling/hating ALPS, Tip Washer ALP, Promega MagnaBot (II) ALP accessory, Bar Code Reader ALP.

Initial setup for a new, not previously programmed project is $500 + consumables. For a previously programmed project the cost is $250 + consumables. Please contact directly for Project Costs.

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Biochemistry & Molecular Biology
Price on request

Custom Projects

We do (and can) perform multistep customised solutions in special circumstances. Please eMAIL us with your query.

Custom Projects

We do (and can) perform multistep customised solutions in special circumstances. Please eMAIL us with your query.

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July 21, 2018

Positive review received for Biochemistry & Molecular Biology:

"The Center was great to work with. All work was done promptly and very well. I would definitely recommend their services! The Director, Andor Kiss, is a fantastic colleague."

2018-05-29 06:33:18 -0700

Net Promoter Score of 10 received for Biochemistry & Molecular Biology.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-04-09 10:57:12 -0700

Net Promoter Score of 9 received for 16S rRNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 7.
2018-03-01 05:58:53 -0800

Net Promoter Score of 10 received for 16S rRNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9,
2017-07-05 15:48:19 -0700

Net Promoter Score of 10 received for 16S rRNA Sequencing.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
2017-05-15 15:07:35 -0700

Net Promoter Score of 10 received for 16S rRNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-05-15 14:57:46 -0700

Net Promoter Score of 10 received for Illumina Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
March 10, 2017

Positive review received for Illumina Sequencing:

"1) The data was of high-quality, and we are progressing nicely with the bioinformatics analyses. 2) The data was generated ahead of schedule. 3) Andor Kiss was a pleasure to work with. Andrew Schuerger"

2016-12-09 10:41:41 -0800

Net Promoter Score of 10 received for Illumina Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2016-06-21 15:26:06 -0700

Net Promoter Score of 10 received for Illumina Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
May 20, 2015

Positive review received for Illumina Sequencing:

"They are pressional."

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