Center for Bioinformatics & Functional Genomics
9.9/10 Overall Rating
100% Positive
23 Orders Completed
Oxford, Ohio, US
The CBFG is the onsite core molecular biology and bioinformatics resource at Miami University (Oxford, OH). We are focused mainly on DNA and protein analysis, but also provide expertise in robotics liquid handling.
Training in the CBFG supports a number of important programs at Miami University. These include the interdisciplinary Cell, Molecular, and Structural Biology (CMSB) program and the Undergraduate Research and Mentoring (URM) program funded by the National Science Foundation.
BluePippin Isolation of DNA fragments for construction of NGS libraries and/or sub-cloning. A variety of size isolation sizes available from 100bp to 50 Kbp.
We will perform basic bioinformatics analysis for some projects on a case-by-case basis. The scope and price will be evaluated on a per-project basis. Example: 16S amplicon project would be $350.
We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the V4 (515f/806r) region. Currently, we use the EMP primer design (old design, not the new). Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis unless added separately; but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.
We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the SSU (1391f/Br) region. Currently, we use the EMP primer design. Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis, but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.
We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the ITS region. Currently, we use the primer design from (Smith, D. P. & Peay, K. G. Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing. PLoS One 9, e90234 (2014)). Contact for pricing - usually between $20 to $30 per sample - depending on the number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis unless added separately, but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.
MiSeq Sequencing Only. We can perform most next generation sequencing protocols suitable for the MiSeq platform. These would include dual INDEX Nextera amplicon sequencing (including 12 bp GOLAY INDEX 16S,18S and ITS emp Protocol), microbial genome sequencing, bacterial metagenomics, RNA-Seq (limited scope). Generally, we run and load "ready-to-go" libraries, but will consider library construction on a case by case basis. Please contact us directly to discuss total project costs.
For a 2x300 v2 MiSeq = $1250
For a 2x50 v2 MiSeq = $950
For a 2x500 v2 MiSeq = $1400
For v3 kits please contact (Illumina pricing is in flux).
We run analysis of BigDye 3.1 Terminator Cycle Sequencing (Sanger) on both ABI 3130xl and ABI 3730 Capillary Electrophoresis instruments. We can also analyze GeneMapper with Dye Set D and G5. Data is provided as .ab1, .phd.1 (Sanger) or .fsa (GM) files.
Individual samples are accepted and analyzed in groups of 16, or 48. Full plate (96 samples) are discounted at $213. No minimum numbers of samples are required. We accept the samples as post-run cleaned & dried down. Please see our recommend protocol (this site, or contact Dr. Kiss). We will add Hi-Di Formamide (20 microL) and perform the CE analysis.
Fragmentation of DNA for downstream use in NGS based protocols. Shearing will be done on a Covaris M220 Sonicator. We highly recommend combining shearing with a QC check on a HS DNA Chip (Bioanalyzer2100).
High Sensitivity DNA Chip Kit Analysis by Bioanalyzer2100. Samples can be sized from 50 to 7000 kbps, 11 samples per chip (gel), quantitative range (concentration) is 5-500 pg/μl.
Analysis of total RNA by RNA Pico 6000 Bioanalyzer2100. Eleven samples can be done on a single chip. Please ensure samples are in the range of 250–5000 pg/μl - minimum of 5 μl is required for analysis.
Custom Robotics Protocols on a epMotion 5073m NGS workstation.
Initial setup for a new, not previously programmed project is $500 + consumables. For a previously programmed project the cost is $250 + consumables. Please contact directly for Project Costs.
Custom Projects
We do (and can) perform multistep customised solutions in special circumstances. Please eMAIL us with your query.
"I worked with Andor Kiss, he did a great job start to finish. He reran all samples that did not show signals on the first run and performed a complete optimization to figure out the issues. I really appreciated his patience and honesty!"
"The Center was great to work with. All work was done promptly and very well. I would definitely recommend their services! The Director, Andor Kiss, is a fantastic colleague."
"1) The data was of high-quality, and we are progressing nicely with the bioinformatics analyses. 2) The data was generated ahead of schedule. 3) Andor Kiss was a pleasure to work with. Andrew Schuerger"
"They are pressional."
Center for Bioinformatics & Functional Genomics has not received any endorsements.