Center for Bioinformatics & Functional Genomics

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the V4 (515f/806r) region. Currently, we use the EMP primer design (old design, not the new). Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis unless added separately; but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the SSU (1391f/Br) region. Currently, we use the EMP primer design. Contact for pricing - usually between $20 to $30 per sample - depending on number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis, but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.

We perform Library Construction, QC, and Data Acquisition (NGS Sequencing on Illumina MiSeq Platform) for the ITS region. Currently, we use the primer design from (Smith, D. P. & Peay, K. G. Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing. PLoS One 9, e90234 (2014)). Contact for pricing - usually between $20 to $30 per sample - depending on the number of samples. There is a hard floor for pricing (minimum of about $2500 per run). We do not perform the bioinformatics analysis unless added separately, but will deliver demultiplexed Read1 and Read2 FASTQs from each sample. Usual turn-around is under 30 days.

MiSeq Sequencing Only. We can perform most next generation sequencing protocols suitable for the MiSeq platform. These would include dual INDEX Nextera amplicon sequencing (including 12 bp GOLAY INDEX 16S,18S and ITS emp Protocol), microbial genome sequencing, bacterial metagenomics, RNA-Seq (limited scope). Generally, we run and load "ready-to-go" libraries, but will consider library construction on a case by case basis. Please contact us directly to discuss total project costs.

For a 2x300 v2 MiSeq = $1250
For a 2x50 v2 MiSeq = $950
For a 2x500 v2 MiSeq = $1400

For v3 kits please contact (Illumina pricing is in flux).

We run analysis of BigDye 3.1 Terminator Cycle Sequencing (Sanger) on both ABI 3130xl and ABI 3730 Capillary Electrophoresis instruments. We can also analyze GeneMapper with Dye Set D and G5. Data is provided as .ab1, .phd.1 (Sanger) or .fsa (GM) files.
Individual samples are accepted and analyzed in groups of 16, or 48. Full plate (96 samples) are discounted at $213. No minimum numbers of samples are required. We accept the samples as post-run cleaned & dried down. Please see our recommend protocol (this site, or contact Dr. Kiss). We will add Hi-Di Formamide (20 microL) and perform the CE analysis.

Fragmentation of DNA for downstream use in NGS based protocols. Shearing will be done on a Covaris M220 Sonicator. We highly recommend combining shearing with a QC check on a HS DNA Chip (Bioanalyzer2100).

High Sensitivity DNA Chip Kit Analysis by Bioanalyzer2100. Samples can be sized from 50 to 7000 kbps, 11 samples per chip (gel), quantitative range (concentration) is 5-500 pg/μl.

Analysis of total RNA by RNA Pico 6000 Bioanalyzer2100. Eleven samples can be done on a single chip. Please ensure samples are in the range of 250–5000 pg/μl - minimum of 5 μl is required for analysis.

Custom Robotics Protocols on a Beckman-Coulter BiomekFX Liquid Handling Robot. Dual Pod (AP96 and SPAN8), orbital shaker ALP, SPE vacuum ALP, chilling/hating ALPS, Tip Washer ALP, Promega MagnaBot (II) ALP accessory, Bar Code Reader ALP.

Initial setup for a new, not previously programmed project is $500 + consumables. For a previously programmed project the cost is $250 + consumables. Please contact directly for Project Costs.