Celplor LLC

Cell lines available:
- HGK293
- COS-7
- 3T3

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Assay design and optimization are the core of biomedical research. We have been working on basic research projects for over twenty years. Our scientists have published numerous original research papers in high impact journals in their fields. Our research fields cover almost every main subjects of modern biology and we are familiar with most of commonly used assays in these fields. We can work with you as a team for your new assay development. We can help you design the assay, make reagents and perform assay optimization. By leveraging our years of experiences in basic research, you can significantly shorten the time for you assay development and become more productive and competitive.

One of the important steps in cell based assay development is creating a cell line stably expressing your gene of interest at high level. It is usually a time-limiting step of your project. Why risk wasting your precious time by assigning this critical task to someone with limited experiences? With your expression vector ready, we can deliver your monoclonal stable cell line with highest expression of your gene of interest within 2-3 months.

Cell lines available:
- HGK293
- COS-7
- 3T3

Turnaround time: 2-3 months

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Capabilities:
- Kit-based, custom designed ELISA
- Sandwich, competitive, indirect
- Homogenization of cell/tissue lysates
- Transfection/Transduction of mammalian cells
- Analyze serum, plasma, cells, tissues
- In-house mouse/rat serum and tissues
- Analyze chromogenic substrates

Equipment:
- Bio-Rad microplate reader
- Multi-channel pipets

Number of samples processed in a single experiment: 96-well plate

Typical turn-around time: 5-10 business days

ELISA assay development, validation and sample processing

Over 100 established ELISA assays for human, mouse and rat proteins, including: interleukins, interferons, TGFs, EGF, etc.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Long range PCR and Southern blot screening including primer and probe design.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

We provide mouse tail genotyping service with affordable price and fast turnaround. Results are usually available within 48 hrs after samples are received. $5.50 per sample with $2.00/extra reaction. Electronic image of the gel is included in the report. No minimum number of samples is required for each order.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Regular cDNA cloning starts from $250.00 depending on the size of the insert. Price includes primer design and synthesis, sequencing verification of 5' and 3' ends of insert. No charge if the construct cannot be delivered.

Deliverables:
- one mini-prep
- one glycerol stock
- electronic report with sequencing chromatograms
- samples returned upon request

Turnaround time: 10-15 business days

Please include the following in your quote request:
- sample size
- vector
- template
- concentration

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Our protein scientists have been working on protein expression and purification for more than twenty years. We can start your project even you have nothing in hand but the name of your protein.

You are flexible in choosing:
• expression system (Bacteria, insect or mammalian)
• tag (6xHis, Flag, HA, GST, MBP, etc)
• tag location (N or C terminus)
• tag removal

We will work with you to set milestones for your project. We will provide you detailed results of each milestones to ensure you are confident for continuing the project.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Southern blot is the golden standard for the identification of positive ES clones when generating knock-out and knock-in mice. However, most of the labs use radioactive probes which are hazardous to both environment and experimenter. Moreover, high background and low specificity are the common problems encountered in Southern blot. Weeks or even months are wasted in the optimization of experimental conditions. We are specialized in Southern blot analysis. We use non-radioactive approach with same sensitivity as radioactive protocol. Turnaround time is only one month from probe design, probe screen to positive ES clone identification.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Stable cell line development
One of the important steps in cell based assay development is creating a cell line stably expressing your gene of interest at high level. It is usually a time-limiting step of your project. Why risk wasting your precious time by assigning this critical task to someone with limited experiences? With your expression vector ready, we can deliver your monoclonal stable cell line with highest expression of your gene of interest within 2-3 months.

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.

Capabilities:
- Homogenization of cell, tissue lysates
- Transfection/Transduction of mammalian cells
- In-house cell lines (HEK293, 3T3, CHO) and tissue banks (rat/mouse)
- Protein concentration determination (DC™ Protein Assay)
- Minimum protein concentration - 1µg/uL
- Strip/reprobe membranes
- Commonly used antibodies available (anti-actin, HRP-labeled anti-mouse/rabbit secondary)
- Optimize protocols for particular antigen/target

Visualization:
- Chemiluminescent substrates on x-ray film (Fuji)
- Ponceau S staining

Equipment:
- Bio-Rad
- Wet transfers

Number of samples in a single run: 9

Our protein scientists have rich experiences in Western blot Analysis. Thousands of gels have been completed and results are published in peer-reviewed journals. Proprietary know-hows and reagent components significantly lower the background signals and enhance positive signals.

Turnaround time: 5 business days

Please include the following in your quote request:
- concentration of samples

Please include the following in your sample shipment:
- primary Ab (we do not have collections of primary Abs, researcher must provide)

Deliverables:
- pictures of the gel
- samples returned upon request

Note: Customer has to provide QC data of all the samples and reagents as specified by Celplor prior to submission. Celplor will verify the quality of the received materials. Customer will be responsible for any extra cost of the project resulted from inaccurate QC data and/or low quality material submitted.