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Cellular Imaging Core at Lake Nona

Orlando, Florida, US

The Cell Imaging core at Sanford-Burnham’s Orlando campus houses state of the art imaging instruments and extensive digital image acquisition and process services, and provides guidance on experiment design, investigator training and use of the instruments, or full-service imaging. It also offers assistance in troubleshooting and optimizing experiments to ensure the highest data is acquired.

The Cell Imaging Core at Lake Nona broadly supports research programs by providing access to sophisticated microscopes for digital imaging, as well as training, assistance and guidance, helping scientists from any institution.

Equipment and Resources:

  • Nikon A1RSI Spectral Confocal Microscope: laser point scanner and high-speed resonant scanning mirrors, A1-DUV GAsP Detector and AOTF Control with 7 Laser Lines (402, 458, 476, 488, 514, 561 and 637nm) on a Ti-E fully motorized inverted microscope with Perfect... Show more »

The Cell Imaging core at Sanford-Burnham’s Orlando campus houses state of the art imaging instruments and extensive digital image acquisition and process services, and provides guidance on experiment design, investigator training and use of the instruments, or full-service imaging. It also offers assistance in troubleshooting and optimizing experiments to ensure the highest data is acquired.

The Cell Imaging Core at Lake Nona broadly supports research programs by providing access to sophisticated microscopes for digital imaging, as well as training, assistance and guidance, helping scientists from any institution.

Equipment and Resources:

  • Nikon A1RSI Spectral Confocal Microscope: laser point scanner and high-speed resonant scanning mirrors, A1-DUV GAsP Detector and AOTF Control with 7 Laser Lines (402, 458, 476, 488, 514, 561 and 637nm) on a Ti-E fully motorized inverted microscope with Perfect Focus System (PFS). High-speed resonant scanning or high-resolution point scanning. It has a Nikon Qi1 CCD Camera for wide-field fluorescence, and capability for live cell imaging, stage and objective heating, and gas control. Runs on Nikon Elements.

  • Nikon Inverted Total Internal Reflection Fluorescence Microscope: Brightfield, DIC and Widefield Fluorescence and (TIRF) fully motorized microscope with PFS (Perfect Focus System). Equipped for FRET and Ca2+ imaging, with a cooled monochrome EMCCD camera (QuantEM 16bit 512x512, Photometrics) and a cooled monochrome CCD camera (CoolSNAP HQ2 14bit 1392x1040, Photometrics). Lamdba DG-5 Xenon lamp, Mercury Intensilight, Ar-ion laser emitting at 488 and 514 nm and diode laser emitting at 561 nm. Runs on Nikon Elements Software.

  • A non-motorized routine Olympus inverted wide-field fluorescence and diascopic light microscope equipped with a SPECTRA X, a State of the Art Solid State Lighting Instrument that uses an arc lamp that provides a cool, stable, robust, spectrally pure light and allows the user to make his or her own choices about bandpass during use in the field is also available. This system has six independent sources, one for each color band. The microscope was also equipped with a QImaging Retiga 4000R digital camera, which provides higher sensitivity and resolution that makes it ideal for automated imaging applications.

  • A non-motorized routine Olympus inverted wide-field fluorescence and diascopic light microscope equipped with a SPECTRA X, a State of the Art Solid State Lighting Instrument that uses an arc lamp that provides a cool, stable, robust, spectrally pure light and allows the user to make his or her own choices about bandpass during use in the field is also available. This system has six independent sources, one for each color band. The microscope was also equipped with a QImaging Retiga 4000R digital camera, which provides higher sensitivity and resolution that makes it ideal for automated imaging applications.

  • A Nikon Eclipse LV100N Polarizing Microscope: It can be used for the quantitative assessment of myocardial collagen. Polarized light microscopy can also be used to study a wide range of physicochemical properties. Materials and tissues can be characterized and identified by optical properties such as refractive index and birefringence and other phenomena such as particle size, particle shape, color, twinning and the presence of inclusions.

  • A Zeiss PALM Laser capture microdissection System: Using this instrument user can collect tissue or cells for further analysis with extreme precision and without physical contact.

  • Two Nikon Biostations, which are enclosed environmental systems capable of 2-channel fluorescence or bright filed illumination.

  • An FEI Morgagni TEM equipped with SC1000 ORIUS™ CCD camera, which is the latest generation of large format retractable and fiber-optical coupled CCD cameras; it offers a high speed (>14 fps - frames per second) image viewing mode, the SC1000 can output high quality (dark and gain corrected) images via a digital video stream.

  • Two dedicated image analysis workstations are available with Nikon Elements, ImagePro, Volocity, ImageJ and HALO. HALO offers 14 image analysis algorithms from Indica labs allowing molecular assay, morphological feature detection and quantification of scientific images. The algorithms are integrated with Aperio Spectrum images and will also work on images acquired from other systems.

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Cellular Imaging Core at Lake Nona has not listed any services.

Bright-Field Microscopy
Price on request
  • Brightfield and Darkfield
  • Phase contrast
  • Nomarski Differential Interference Contrast (DIC)
  • Live Cell and Fixed Specimen
  • Brightfield and Darkfield
  • Phase contrast
  • Nomarski Differential Interference Contrast (DIC)
  • Live Cell and Fixed Specimen
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Confocal Microscopy
Price on request

Nikon A1RSI Spectral Confocal Microscope with standard and high-speed resonant scanning mirrors, a multi-detector unit equipped with 2 GaAsP PMTs and 2 high sensitivity PMTs and AOTF Control with 7 Laser Lines (402, 458, 476, 488, 514, 561 and 637nm) on a Ti-E fully motorized inverted microscope with Perfect Focus System,... Show more »

Nikon A1RSI Spectral Confocal Microscope with standard and high-speed resonant scanning mirrors, a multi-detector unit equipped with 2 GaAsP PMTs and 2 high sensitivity PMTs and AOTF Control with 7 Laser Lines (402, 458, 476, 488, 514, 561 and 637nm) on a Ti-E fully motorized inverted microscope with Perfect Focus System, white-light TIRF Illuminator, a Nikon Qi1 CCD Camera for wide-field fluorescence, and capability for live cell imaging with or without continuous superfusion, stage and objective heating, and gas control.

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Confocal Microscopy Training
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Fluorescence Microscopy Training
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Laser Capture Microdissection
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Laser microdissection experiments can be performed using the Zeiss PALM Laser capture microdissection System in the Core. Using this instrument user can collect tissue or cells for further analysis with extreme precision and without physical contact.

Laser microdissection experiments can be performed using the Zeiss PALM Laser capture microdissection System in the Core. Using this instrument user can collect tissue or cells for further analysis with extreme precision and without physical contact.

« Show less
Microscopy and Imaging Fundamentals Training
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Polarized Light Microscopy
Price on request

A Nikon Eclipse LV100N Polarizing Microscope is offered in the Facility, it can be used for the quantitative assessment of myocardial collagen and can involve a multitude of disciplines that seek to unravel the consequences of complex interactions between organisms and the physical world. Polarized light microscopy can also be... Show more »

A Nikon Eclipse LV100N Polarizing Microscope is offered in the Facility, it can be used for the quantitative assessment of myocardial collagen and can involve a multitude of disciplines that seek to unravel the consequences of complex interactions between organisms and the physical world. Polarized light microscopy can also be used to study a wide range of physicochemical properties. Materials and tissues can be characterized and identified by optical properties such as refractive index and birefringence and other phenomena such as particle size, particle shape, color, twinning and the presence of inclusions.

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Stereomicroscopy
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Transmission Electron Microscopy (TEM)
Transmission Electron Microscopy services
Price on request

FEI Morgagni TEM equipped with SC1000 ORIUS™ CCD camera, which is the latest generation of large format retractable and fiber-optical coupled CCD cameras; it offers a high speed (>14 fps - frames per second) image viewing mode, the SC1000 can output high quality (dark and gain corrected) LIVE images via a digital video stream.

FEI Morgagni TEM equipped with SC1000 ORIUS™ CCD camera, which is the latest generation of large format retractable and fiber-optical coupled CCD cameras; it offers a high speed (>14 fps - frames per second) image viewing mode, the SC1000 can output high quality (dark and gain corrected) LIVE images via a digital video stream.

« Show less
TIRF
Total Internal Reflection Microscopy
Price on request

Fully motorized combined dual laser (488 and 561nm) total internal reflection (TIRFM) and wide-field fluorescence microscope (Nikon TiE) equipped with a Lambda DG-5 excitation source/controller suitable for Ca2+-imaging and high speed FRET analyses, two cameras (high speed Photometrics QuantEM EMCCD and high-resolution HQ2 CCD camera), and live cell imaging capability.

Fully motorized combined dual laser (488 and 561nm) total internal reflection (TIRFM) and wide-field fluorescence microscope (Nikon TiE) equipped with a Lambda DG-5 excitation source/controller suitable for Ca2+-imaging and high speed FRET analyses, two cameras (high speed Photometrics QuantEM EMCCD and high-resolution HQ2 CCD camera), and live cell imaging capability.

« Show less
Widefield Fluorescence Microscopy
Price on request

Non-motorized routine Olympus inverted wide-field fluorescence and diascopic light microscope equipped with a SPECTRA X, a State of the Art Solid State Lighting Instrument that uses an arc lamp that provides a cool, stable, robust, spectrally pure light and allows the user to make his or her own choices about bandpass during use... Show more »

Non-motorized routine Olympus inverted wide-field fluorescence and diascopic light microscope equipped with a SPECTRA X, a State of the Art Solid State Lighting Instrument that uses an arc lamp that provides a cool, stable, robust, spectrally pure light and allows the user to make his or her own choices about bandpass during use in the field is also available. This system has six independent sources, one for each color band. The microscope was also equiped with a QImaging Retiga 4000R digital camera, which provides higher sensitivity and resolution that makes it ideal for automated imaging applications.

« Show less
Biology
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Cells and Tissues
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Biospecimens
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Tissue Processing
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Tissue Preparation
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Imaging & Spectroscopy
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Imaging and Microscopy Training
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Microscopy
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Electron Microscopy
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Optical Microscopy
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Transmitted Light Microscopy
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Fluorescence-Based Microscopy
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