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Cellomatics BioSciences Ltd

2 Orders Completed
Nottingham, GB

About Cellomatics BioSciences Ltd

Cellomatics Biosciences Ltd. is a laboratory-based Contract Research Organisation (CRO) specialised in Oncology/Immuno-Oncology, Immunology/Inflammation and Respiratory therapeutic areas. We provide bespoke pre-clinical/early discovery phase laboratory services to support academic groups,... Show more »

Cellomatics Biosciences Ltd. is a laboratory-based Contract Research Organisation (CRO) specialised in Oncology/Immuno-Oncology, Immunology/Inflammation and Respiratory therapeutic areas. We provide bespoke pre-clinical/early discovery phase laboratory services to support academic groups, pharmaceutical and biotechnology companies in their R&D studies and biomarker research. Backed by the company's expertise in providing preclinical drug development services and as part of its growth strategy, Cellomatics BioSciences is expanding to be an innovative biopharmaceutical company. Our objective is to generate a growing pipeline of highly differentiated, potential best-in-class therapeutics to drive the development of novel drug products for treatment of life-threatening diseases with unmet medical need. Our commitment is to develop and deliver therapeutic compounds with improved efficacy and/or adverse effect profile in comparison with the currently available approved standard of care products or any other products in development.

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Our Services (39)


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Drug Target Validation

Price on request

Target validation includes the following stages:

  • Discovering a biomolecule of interest
  • Investigating the expression of the molecular target within the tissue of interest
  • Evaluating its potential as a target to understand if it is directly involved in the disease process
  • Designing a bioassay to measure biological... Show more »

Target validation includes the following stages:

  • Discovering a biomolecule of interest
  • Investigating the expression of the molecular target within the tissue of interest
  • Evaluating its potential as a target to understand if it is directly involved in the disease process
  • Designing a bioassay to measure biological activity to verify if target modulation produces the desired therapeutic effect.
  • Constructing a high-throughput screen.
  • Performing screening to find hits and evaluating the hits

We offer a range of strategies and capabilities for modulating gene expression in vitro.

These include but are not limited to the use of antibodies, negative dominant controls, antisense oligonucleotides, ribozymes, and small-interfering RNAs.

Our services to support target validation and discovery:

  • We can offer support and assay design for protein and novel target expression in a range of normal and diseased tissue, including:
    • IHC and novel marker assay development & co-localisation studies
    • phosphorylated marker assay development
  • Low-throughput target validation assay that represents biology
  • Molecular level
    • Screen enzyme inhibitors or activators
  • Cellular Level
    • Verify the involvement of the protein in the disease state (often use gene silencing siRNAs).
    • Understand the protein pathways and interactions.
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Drug Target Identification

Price on request

Target identification/discovery is the first stage of drug development process. The key objective is to identify a novel or a putative therapeutic target for a disease with unmet medical need. Often proteins including enzymes, cell signalling receptors, structural proteins and regulatory factors are the most common targets.... Show more »

Target identification/discovery is the first stage of drug development process. The key objective is to identify a novel or a putative therapeutic target for a disease with unmet medical need. Often proteins including enzymes, cell signalling receptors, structural proteins and regulatory factors are the most common targets. However, nucleic acids such as mRNA, carbohydrates and lipids are increasingly being recognised as therapeutic targets.

Target identification can be based on:

  • a published (peer-reviewed) data demonstrating a link between the target and a disease of interest: literature target, patents etc.
  • ‘Omic’ studies: Transcriptional profiling and proteomics data that leads to the discovery of genes/proteins with differential expression patterns in diseased states
  • Evidence of a novel biology that regulates the disease pathway or the target of interest.
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Biomarker Analytical Validation

Price on request

Validation is the process of evaluating the reliability, reproducibility and accuracy of the biomarker across a range of experimental conditions. Qualification process provides an evidentiary link between the biomarker and biological processes/clinical end points.

Following validation and qualification, a biomarker can be used... Show more »

Validation is the process of evaluating the reliability, reproducibility and accuracy of the biomarker across a range of experimental conditions. Qualification process provides an evidentiary link between the biomarker and biological processes/clinical end points.

Following validation and qualification, a biomarker can be used to diagnose the risk, presence and prognosis of a disease in an individual. In evaluating potential drug therapies, a biomarker may be used as a surrogate for a natural endpoint such as survival or irreversible morbidity. If a treatment alters the biomarker, which has a direct connection to improved health, the biomarker serves as a surrogate endpoint for evaluating clinical benefit. Some of the main areas in which molecular biomarkers are used in the drug development process are: early drug development studies, safety studies, proof of concept studies, and molecular profiling.

We offer assay development and validation for prognostic/predictive clinical biomarkers and pathway activation biomarkers:

  • Mutliplex immunoassay
  • Multiplex nucleic acid testing
  • Western Blot
  • Clinical Chemistry assays
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Multiplex Immunoassays

Price on request

Multiplex immunoassays allow simultaneous detection and quantification of multiple analytes in a single sample from a variety of biological specimens including but not limited to serum, plasma, CSF, cell culture supernatants, sputum.

xMAP Technology has application in serological assays by easy detection of antibodies. A... Show more »

Multiplex immunoassays allow simultaneous detection and quantification of multiple analytes in a single sample from a variety of biological specimens including but not limited to serum, plasma, CSF, cell culture supernatants, sputum.

xMAP Technology has application in serological assays by easy detection of antibodies. A serology assay enables the detection of an antibody (target) with the use of a capture protein antigen attached to the surface of a microsphere. A detection antibody that incorporates a fluorescent label is used to quantify the amount of target antibody present. This assay is useful when serum antibodies need to be measured, such as for the response to a vaccine, allergic reactions, or autoantibody detection.

It combines the efficiency and sensitivity of traditional ELISAs with high through-put capacity, small sample volume,efficiency in terms of time and cost,high reproducibility and sensitivityand analyte quantification over a wide range of concentrations.

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Multiplex Gene Expression Analysis

Price on request

Multiplex nucleic acid testing allows multiplexed gene expression analysis (RNA targets) and DNA copy number variation analysis, allowing researchers to measure up to 80 genes in a single well. These assays are hybridization-based using the xMAP® Luminex®magnetic beads and performed on 96-well plates. Luminex xMAP® technology... Show more »

Multiplex nucleic acid testing allows multiplexed gene expression analysis (RNA targets) and DNA copy number variation analysis, allowing researchers to measure up to 80 genes in a single well. These assays are hybridization-based using the xMAP® Luminex®magnetic beads and performed on 96-well plates. Luminex xMAP® technology combines advanced fluidics, optics, and digital signal processing with fluorescently dyed microspheres to enable the quantitation of multiple nucleic acid from a single sample. There are >17,000 targets for RNA and >700 targets for DNA available for customisation.

We have a number of assays that are applicable to clinical diagnostics, infectious diseases and genetic (pharmacogenetic testing). Luminex xTAG Technology products provide accurate, flexible, low-cost bead arrays. They combine any set of up to 150 nucleic acid tests and multiplex them in a single reaction. xTAG assays are run on the Luminex xMAP® systems, which use lasers or LEDs to read color coded microspheres that attach to specific nucleic acid sequences.

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qRT-PCR

Quantitative reverse transcription polymerase chain reaction
Price on request

(Real-Time Quantitative Reverse Transcription PCR) is a major development of PCR technology that enables reliable detection and measurement of products generated during each cycle of PCR process. This technique became possible after introduction of an oligonucleotide probe which was designed to hybridize within the target... Show more »

(Real-Time Quantitative Reverse Transcription PCR) is a major development of PCR technology that enables reliable detection and measurement of products generated during each cycle of PCR process. This technique became possible after introduction of an oligonucleotide probe which was designed to hybridize within the target sequence. Cleavage of the probe during PCR because of the 5' nuclease activity of Taq polymerase can be used to detect amplification of the target-specific product.

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cDNA Synthesis

Price on request

Total RNA can be isolated from cells in culture or from tissues. RNA can be extracted using Tri-reagent methodology or preparatory RNA isolation kits which typically use a combination of organic and solid phase extraction methodologies.

Either Tri-reagent or a lysis buffer are used for the extraction of RNA samples, which are... Show more »

Total RNA can be isolated from cells in culture or from tissues. RNA can be extracted using Tri-reagent methodology or preparatory RNA isolation kits which typically use a combination of organic and solid phase extraction methodologies.

Either Tri-reagent or a lysis buffer are used for the extraction of RNA samples, which are finally resuspended in Tris-EDTA buffer. The RNA mass is determined using an NanoDrop, purity assessed from the 260/280nm and 230/260 ratio. Following extraction, RNA is processed with a Turbo DNA-free kit to reduce any residual genomic DNA (gDNA) contamination prior to reverse transcription and production of copy DNA (cDNA) using the High Capacity cDNA Reverse Transcription Kit.

Once formed cDNA samples can be probed using quantitative PCR (QPCR) and commercially available TaqMan probes from Applied Biosystems, currently there are over 8 million predesigned TaqManTM assays available .

Typically QPCR require a suitable endogenous control - a stable gene that can be used to make relative measures against. Commercially available endogenous control TaqMan probes are available such as beta-glucuronidase (GUSB), hypoxanthine-guanine hosphoribosyltransferase HPRT1 (HGPRT), beta-2-microglobulin (B2M) and human glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Selection of an appropriate control requires screening cDNA for a range of endogenous controls and analysing the data using GeneNorm. Once identified endogenous control genes can be determined alongside a gene of interest to determine changes in gene expression. This enables normalising of target gene expression levels using the equation 2(-ΔΔCt), where ΔΔCt = ΔCt (treated) -ΔCt (control; media) and this calculates fold-change in the gene expression.

A dual assay methodology is used which which enables endogenous control genes and genes of interest to be investigated simultaneously on a sample in a single well - this reduces the quantity of sample required. QPCR experiments are performed on a StepOne Plus instrument using a thermal profile of 2min at 50οC, 10min at 95οC, 50 cycles of 15s at 95οC and 1min at 60οC. Non-template controls were included for all samples.

Non-template controls (NTCs) are determined for all samples and represent a negative control which is run in parallel for each sample, in which the reverse transcriptase enzyme, which converts mRNA to cDNA, is removed. If there is amplification in NTCs it represents genomic DNA contamination and not mRNA expression.

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qPCR

Quantitative PCR
Price on request

Total RNA can be isolated from cells in culture or from tissues. RNA can be extracted using Tri-reagent methodology or preparatory RNA isolation kits which typically use a combination of organic and solid phase extraction methodologies.

Either Tri-reagent or a lysis buffer are used for the extraction of RNA samples, which are... Show more »

Total RNA can be isolated from cells in culture or from tissues. RNA can be extracted using Tri-reagent methodology or preparatory RNA isolation kits which typically use a combination of organic and solid phase extraction methodologies.

Either Tri-reagent or a lysis buffer are used for the extraction of RNA samples, which are finally resuspended in Tris-EDTA buffer. The RNA mass is determined using an NanoDrop, purity assessed from the 260/280nm and 230/260 ratio. Following extraction, RNA is processed with a Turbo DNA-free kit to reduce any residual genomic DNA (gDNA) contamination prior to reverse transcription and production of copy DNA (cDNA) using the High Capacity cDNA Reverse Transcription Kit.

Once formed cDNA samples can be probed using quantitative PCR (QPCR) and commercially available TaqMan probes from Applied Biosystems, currently there are over 8 million predesigned TaqManTM assays available .

Typically QPCR require a suitable endogenous control - a stable gene that can be used to make relative measures against. Commercially available endogenous control TaqMan probes are available such as beta-glucuronidase (GUSB), hypoxanthine-guanine hosphoribosyltransferase HPRT1 (HGPRT), beta-2-microglobulin (B2M) and human glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Selection of an appropriate control requires screening cDNA for a range of endogenous controls and analysing the data using GeneNorm. Once identified endogenous control genes can be determined alongside a gene of interest to determine changes in gene expression. This enables normalising of target gene expression levels using the equation 2(-ΔΔCt), where ΔΔCt = ΔCt (treated) -ΔCt (control; media) and this calculates fold-change in the gene expression.

A dual assay methodology is used which which enables endogenous control genes and genes of interest to be investigated simultaneously on a sample in a single well - this reduces the quantity of sample required. QPCR experiments are performed on a StepOne Plus instrument using a thermal profile of 2min at 50οC, 10min at 95οC, 50 cycles of 15s at 95οC and 1min at 60οC. Non-template controls were included for all samples.

Non-template controls (NTCs) are determined for all samples and represent a negative control which is run in parallel for each sample, in which the reverse transcriptase enzyme, which converts mRNA to cDNA, is removed. If there is amplification in NTCs it represents genomic DNA contamination and not mRNA expression.

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RNA Extraction and Purification

Price on request

Total RNA can be isolated from cells in culture or from tissues. RNA can be extracted using Tri-reagent methodology or preparatory RNA isolation kits which typically use a combination of organic and solid phase extraction methodologies.

Either Tri-reagent or a lysis buffer are used for the extraction of RNA samples, which are... Show more »

Total RNA can be isolated from cells in culture or from tissues. RNA can be extracted using Tri-reagent methodology or preparatory RNA isolation kits which typically use a combination of organic and solid phase extraction methodologies.

Either Tri-reagent or a lysis buffer are used for the extraction of RNA samples, which are finally resuspended in Tris-EDTA buffer. The RNA mass is determined using an NanoDrop, purity assessed from the 260/280nm and 230/260 ratio. Following extraction, RNA is processed with a Turbo DNA-free kit to reduce any residual genomic DNA (gDNA) contamination prior to reverse transcription and production of copy DNA (cDNA) using the High Capacity cDNA Reverse Transcription Kit.

Once formed cDNA samples can be probed using quantitative PCR (QPCR) and commercially available TaqMan probes from Applied Biosystems, currently there are over 8 million predesigned TaqManTM assays available .

Typically QPCR require a suitable endogenous control - a stable gene that can be used to make relative measures against. Commercially available endogenous control TaqMan probes are available such as beta-glucuronidase (GUSB), hypoxanthine-guanine hosphoribosyltransferase HPRT1 (HGPRT), beta-2-microglobulin (B2M) and human glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Selection of an appropriate control requires screening cDNA for a range of endogenous controls and analysing the data using GeneNorm. Once identified endogenous control genes can be determined alongside a gene of interest to determine changes in gene expression. This enables normalising of target gene expression levels using the equation 2(-ΔΔCt), where ΔΔCt = ΔCt (treated) -ΔCt (control; media) and this calculates fold-change in the gene expression.

A dual assay methodology is used which which enables endogenous control genes and genes of interest to be investigated simultaneously on a sample in a single well - this reduces the quantity of sample required. QPCR experiments are performed on a StepOne Plus instrument using a thermal profile of 2min at 50οC, 10min at 95οC, 50 cycles of 15s at 95οC and 1min at 60οC. Non-template controls were included for all samples.

Non-template controls (NTCs) are determined for all samples and represent a negative control which is run in parallel for each sample, in which the reverse transcriptase enzyme, which converts mRNA to cDNA, is removed. If there is amplification in NTCs it represents genomic DNA contamination and not mRNA expression.

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In vitro Toxicity Testing

Price on request

Drugs research and development cost every year over $145 billion in US only. One of the key contributors to these rising costs is unacceptable drug safety profile either detected during Phase 1 Clinical Trial (50-60%) or during Phase IV Post marketing surveillance where some severe side effects were unnoticed during the phase I... Show more »

Drugs research and development cost every year over $145 billion in US only. One of the key contributors to these rising costs is unacceptable drug safety profile either detected during Phase 1 Clinical Trial (50-60%) or during Phase IV Post marketing surveillance where some severe side effects were unnoticed during the phase I clinical trial. Of note, it has been described that in 30% of the studied cases the animals showed a toxic effect on a different organ compared to human (https://www.gwern.net/docs/dnb/2000-olson.pdf)

Main point of Preclinical testing is to determine the potential drug adverse effects, toxicity and drug-drug interactions before the drug is tested in either animal models or Phase 1 clinical trial. Hepato and renal in vitro toxicity tests are fundamental for pre-screening drug compounds to reduce costs for in vivo testing and Clinical Trials.
Moreover, it should be considered that the animal model might not mirror completely the human model, a good example is the expression of cytochrome P450. Hence the need to identify physiologically-relevant hepatic and kidney cellular models.
We offer a vast selection of 2D and 3D cellular models together with biochemical, metabolic and genetic assays to detect drug toxicity, ADME, pharmacokinetic and toxicogenomics.

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Immunohistochemistry (IHC)

Price on request

Cellomatics provides Contract Histology Services to support non-regulatory pre-clinical histology and medical research studies in tissue from humans and other species.

We offer:

  • Tissue processing, embedding in paraffin and sectioning at different thickness, staining with standard and/or specific dyes. All process or just... Show more »

Cellomatics provides Contract Histology Services to support non-regulatory pre-clinical histology and medical research studies in tissue from humans and other species.

We offer:

  • Tissue processing, embedding in paraffin and sectioning at different thickness, staining with standard and/or specific dyes. All process or just parts of the process, i.e. only cutting.
  • Setting protocols - titration and optimisation of antibodies
  • Image analysis and reporting
  • DNA/RNA extraction from FFPE
  • parallel analysis of analytes/genes with xMAP and localisation in tissues with IHC

We can undertake an extensive range of specialised staining methods to identify specific tissues, cell types and tissue/cell constituents including hematoxylin, eosin, PAS, Masson's Trichrome, Reticulin, Giemsa, Van Geisson, MSB, PTAH, OGF, silver impregnation, Oil Red O, Alcian blue, Congo red, Toluidine blue and many more. All specialised stains are accompanied with appropriate controls.

Cellomatics has extensive experience in providing immunohistochemistry services to screen healthy and diseases tissues using an extensive range of antibodies directed against specific biomarkers or against cell signalling proteins.

We support Target Validation and Target Distribution Studies by demonstrating and quantifying the expression of a particular target within cells/tissues which can be used either as predictive or prognostic tissue biomarkers in tissues originating from preclinical animal models or from clinical trials. Further, we have the capabilities to optimise and validate IHC immune-oncology and respiratory biomarker assays together with quantitative analysis to provide valuable scientific insights.

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Mammalian Cell Culture

Price on request

Our cell culture services include primary cultures of human origin as well as established cell lines. This facilitates the production of natural or recombinant proteins or for use in biological assays such as wound healing bioassays, immunomodulation, cell migrations, proliferation/viability, or investigating the inflammatory... Show more »

Our cell culture services include primary cultures of human origin as well as established cell lines. This facilitates the production of natural or recombinant proteins or for use in biological assays such as wound healing bioassays, immunomodulation, cell migrations, proliferation/viability, or investigating the inflammatory mediator response. Our labs are equipped with Class ll biological safety cabinets, fridges, freezers and CO2 incubators. All routine cells are cultured without the use of antibiotics, using a variety of media with additives and serum-free media as appropriate. All cells are tested for the presence of Mycoplasma contamination.

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Lead Identification and Validation

Price on request

Lead Identification and Validation Services

Lead Identification and Validation Services

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Drug Discovery

Price on request

Drug Discovery Services

Drug Discovery Services

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Biomarkers

Price on request

Biomarkers Services

Biomarkers Services

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Immunoassays

Price on request

Immunoassays Services

Immunoassays Services

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Bioanalytical Assays

Price on request

Bioanalytical Assays Services

Bioanalytical Assays Services

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Bioanalysis

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Bioanalysis Services

Bioanalysis Services

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Pharmacology

Price on request

Pharmacology Services

Pharmacology Services

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Genotyping and Gene Expression Assays

Price on request

Genotyping Services

Genotyping Services

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DNA Synthesis and Probe Development

Price on request

DNA Synthesis and Probe Development Services

DNA Synthesis and Probe Development Services

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PCR

Price on request

PCR Services

PCR Services

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DNA Services

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DNA Services

DNA Services

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RNA Services

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RNA Services

RNA Services

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Nucleic Acid Services

Price on request

Nucleic Acid Services

Nucleic Acid Services

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Toxicology

Price on request

Toxicology Services

Toxicology Services

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Pharmacology & Toxicology

Price on request

Pharmacology & Toxicology Services

Pharmacology & Toxicology Services

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Drug Discovery & Development

Price on request

Drug Discovery & Development Services

Drug Discovery & Development Services

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Immunostaining

Price on request

Immunostaining Services

Immunostaining Services

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Protein Expression Visualization

Price on request

Protein Expression Visualization Services

Protein Expression Visualization Services

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Protein Services

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Protein Services

Protein Services

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Biochemistry & Molecular Biology

Price on request

Biochemistry & Molecular Biology Services

Biochemistry & Molecular Biology Services

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Cell and Tissue Culture

Price on request

Cell and Tissue Culture Services

Cell and Tissue Culture Services

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Cells and Tissues

Price on request

Cells and Tissues Services

Cells and Tissues Services

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Biology

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Biology Services

Biology Services

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Biostatistics & Bioinformatics

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Biostatistics & Bioinformatics Services

Biostatistics & Bioinformatics Services

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Statistical Analysis

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Statistical Analysis Services

Statistical Analysis Services

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Data Analysis

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Data Analysis Services

Data Analysis Services

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Data Services

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Data Services

Data Services

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Shailendra Singh

Founder & CEO

Cellomatics BioSciences Ltd has not received any reviews.

Cellomatics BioSciences Ltd has not received any endorsements.