Cell Line Genetics (CLG) is a leading provider of multi-species cell line characterization and authentication services and custom assay development for cell/gene therapy, regenerative medicine and cancer research.
CLG has the track record and knowledge to meet the demanding needs of academic research institutes, as biotechnology and pharmaceutical companies worldwide.
The CLG Advantage:
Unparalleled Insight and Experience:
Cell Line Genetics has the required expertise to help with the most difficult research projects. Our insight in the chromosomal anomalies of embryonic and induced pluripotent stem cells is recognized and relied on. .
Clinical experience and rigor:With a highly experienced scientific team, Cell Line Genetics stands at the forefront of the field and offers leading scientists quality services and innovative strategies for the most cutting-edge research trends.
Rapid Turnaround Time:
CLG understands that our clients need accurate and fast results. We guarantee rapid turnaround while consistently delivering accurate results, subject matter insight and publication ready images - rush services are also available.
Customized Services:Cell Line Genetics develops custom, validated assays for Cell Characterization and other research purposes. We specialize in Cytogenetics and DNA-based assays (FISH, aCGH), yet continuously seek to expand our offering based on our customer needs
Confidentiality and Unbiased Results Guaranteed:At Cell Line Genetics, confidentiality and data security is of the utmost importance. From the moment we receive samples to the delivery of reports via our secure, HIPAA-compliant StemCloud™ platform, CLG takes every measure to provide unbiased value and peace of mind to our customers.
Mycoplasma is a large group of tiny bacteria, about 0.3-0.8 um in diameter, all lacking a rigid cell wall belonging to the Mollicultes class of bacteria. Unlike other bacterial contaminates, the media will not change turbidity from a mycoplasma infection and therefore it will go undetected unless the culture is tested for mycoplasma. Due to the lack of a rigid cell wall and their small size, they are very difficult to eliminate once they contaminate a culture. Most antibiotics used in cell culturing, such as penicillin and streptomycin, work by attacking the rigid cell walls in bacteria, therefore since mycoplasma have no rigid cell wall they do not kill the mycoplasma and only mask the infection. The 0.3-0.8 um diameter and flexibility, from not having a rigid cell wall, allow these bacteria to pass through most of the conventional filters used in labs.
The two most common ways mycoplasma infection occurs:
Effects of mycoplasma on cultured cells:
How to prevent mycoplasma:
Mycoplasma PCR Based Detection
Cell Line Genetics’ PCR based Mycoplasma detection is extremely sensitive, as it primes to the highly conserved 16S rRNA region of the mycoplasma’s genome. It detects 19 species of Mycoplasma and 1 species of Acholeplasma in any species of cells. Of those 19 species of mycoplasma, 6 are responsible for between 90-95% of all mycoplasma contamination in cultured stem cells.
AttributesExtremely sensitive PCR detects only the highly conserved 16s rRNA region Detects 19 species of Mycoplasma and 1 species of Acholeplasma Live cells or spent media passaged twice without antibiotics accepted Fast turnaround time, highly accurate, competitively priced
Used to: Detect mycoplasma contamination in cell cultures
Sample Requirements: Live cell culture (T-25 flask) or spent media (1 mL Tube) passaged twice with antibiotic free media
Turnaround: 7-10 business days: RUSH services available
Volume/Batch Discounts are available
Cell Line Genetics offers various fluorescence in situ hybridization (FISH) services to meet your characterization needs.
Standard FISH service
Used to identify small emerging abnormal clones, complex chromosome aberrations, small partial trisomies, small marker chromosomes, and to refine chromosome breakpoints
FISH Validation of aCGH Findings
Confirm aCGH duplications and deletions using fluorescently labeled probes
Transgene Integration Site Mapping
Determine the chromosome band location of your inserted transgene
Custom FISH Probe Construction and Hybridization
Can't find the probe you are looking for? CLG can create gene specific FISH probes to meet your research need.
Array Comparative Genomic Hybridization (aCGH) is a high resolution karyotype analysis solution for the detection of unbalanced structural and numerical chromosomal alterations with high-throughput capabilities. Addition of SNPs can also detect polylpoidy, loss of heterozygosity, and uniparental disomy.
CLG offers two different array comparative genomic hybridization arrays to help characterize your cell lines. Cell Line Genetics utilizes Agilent’s Microarray platform.
SurePrint G3 Human CGH Microarray Kit, 8x60K – High-throughput, low cost alternative to karyotyping. Average coverage every 41KB with increased coverage in Refseq genes (33KB)
SurePrint G3 Human Genome CGH+SNP Microarray Kit, 4x180K – High resolution array with SNPs included. Average coverage every 25KB with increased coverage in ISCA regions (5KB)
Cell Line Genetics offers two levels of STR Profile analysis—STR100 (basic) and STR300 (comprehensive analysis). Both are designed to accommodate your needs throughout all stages of your stem cell research including cell line derivation, propagation, and long term culture. All STR services include STR profile storage in CLG's secure, encrypted database for future profile comparison analysis, and 24 hour access to reports on our secure cloud database at no additional charge. Short tandem repeat polymorphisms (STRs) are detected by utilizing Promega's PowerPlex® 16 multiplex STR system which detects STRs for 15 Codis loci plus Amelogenin—the same system used by the FBI and other government forensic labs.
|Electropherogram (by request)||x||x|
|Results archived in CLG database||x||x|
|Comprehensive interpretation of results||x|
|Profile comparison analysis||x|
|Database cross-referencing analysis||x|
Cell Line Genetics is recognized world-wide for its expertise in multispecies stem cell and cancer cell line karyotyping analysis. Unlike most cytogenetic core facilities focused on clinical testing, Cell Line Genetics is dedicated to providing the stem cell research and regenerative medicine field with the highest quality g-band karyotyping service available with fast turn-around guaranteed. Furthermore, Cell Line Genetics' scientists are rigorously trained and experienced at stem cell genomic stability analysis and identifying structural chromosomal anomalies unique to multipotent and pluripotent stem cell lines—many of which can lead to invasive cell populations. Since the company’s inception, Cell Line Genetics has characterized thousands of adult, embryonic and induced pluripotent stem cells samples from leading academic institutions, government agencies, and industry stem cell labs. Request an estimate today and have Cell Line Genetics become your g-band karyotyping quality assurance partner.
"CLG scientists are very responsible and try to help me to figure out the problem I had in my cell lines."
Cell Line Genetics, Inc has not received any endorsements.