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Cell Imaging and Analysis Network (CIAN)

Montreal, Quebec

The Cell Imaging and Analysis Network (CIAN) provides services in the following fields:
- Automation/High throughput screening (liquid handler, pinning robot)
- Protein expression and antibody production

The CIAN includes also two user-based facilities, where users get training and work autonomously on the equipment:
- Proteomics (2D, DiGE, related protein analyses)
- Cell Imaging (confocal and fluorescence microscopy)

CIAN is designed to facilitate training, enhance interactions, share resources and expertise

    2D PAGE
    Two dimensional polyacrylamide gel electrophoresis
    $11.57 USD
    per Gel

    Users operated (training ensured by the CIAN)
    24 cm $17.39 CAD
    13 cm $14.18 CAD
    11 cm $14.18 CAD
    7 cm $11.94 CAD

    Users operated (training ensured by the CIAN)
    24 cm $17.39 CAD
    13 cm $14.18 CAD
    11 cm $14.18 CAD
    7 cm $11.94 CAD

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    Antigen expression and purification
    $726.81 USD
    per Sample
    Please contact Cell Imaging and Analysis Network (CIAN) for more information regarding this service.
    Confocal microscopy
    $48.45 USD
    per Hour

    Laser scanning confocal microscopy - $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.
    Spinning disk confocal microscopy - $50 CAD per hour, additional $60 CAD per hour for technical assistance

    Laser scanning confocal microscopy - $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.
    Spinning disk confocal microscopy - $50 CAD per hour, additional $60 CAD per hour for technical assistance

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    DIGE
    Differential gel electrophoresis
    Price on request

    Users operated (training ensured by the CIAN)
    A typical DiGE experiment (2 experimental conditions, 3 biological replicates each, pooled internal standard, all 3 dyes) on three 24cm strips / DALT gels would cost 3 * ($16.63 + $16.84) = $100.41 for the sample separation, plus minimally $738.80 for CyDyes, a total of $839.21 CAD.

    Users operated (training ensured by the CIAN)
    A typical DiGE experiment (2 experimental conditions, 3 biological replicates each, pooled internal standard, all 3 dyes) on three 24cm strips / DALT gels would cost 3 * ($16.63 + $16.84) = $100.41 for the sample separation, plus minimally $738.80 for CyDyes, a total of $839.21 CAD.

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    Data storage
    $24.22 USD
    per Sample

    Per 100GB:
    1 month $25.00 CAD
    6 month $120.00 CAD
    12 month $200.00 CAD

    Per 100GB:
    1 month $25.00 CAD
    6 month $120.00 CAD
    12 month $200.00 CAD

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    Differential interference contrast (DIC) microscopy
    $48.45 USD
    per Hour

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance

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    FRET
    Fluorescence resonance energy transfer
    $48.45 USD
    per Hour

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.

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    Fluorescence microscopy
    $48.45 USD
    per Hour

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.
    Meta Spectral Imaging: Using the concept of Emission Fingerprinting, the LSM 510 META detector is able to separate even widely overlapping emission spectra, allowing for more precision in fluorescent imaging. Fluorochromes that resemble other emission... Show more »

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.
    Meta Spectral Imaging: Using the concept of Emission Fingerprinting, the LSM 510 META detector is able to separate even widely overlapping emission spectra, allowing for more precision in fluorescent imaging. Fluorochromes that resemble other emission spectra peaks can be used simultaneously through the technique of linear unmixing

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    Fluorescence recovery after photobleaching (FRAP)
    $48.45 USD
    per Hour

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance

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    Gel staining
    $1.37 USD
    per Gel

    Users operated (training ensured by the CIAN)
    Deep Purple:
    26cm x 20cm $60.82 CAD
    16cm x 16 cm $40.27 CAD
    13cm x 9cm $10.03 CAD
    8.6cm x 7cm $10.03 CAD

    SYPRO Ruby
    26cm x 20cm $146.34 CAD
    16cm x 16cm $73.67 CAD
    13cm x 9cm $18.32 CAD
    8.6cm x 7cm $18.32 CAD

    Silver Show more »

    Users operated (training ensured by the CIAN)
    Deep Purple:
    26cm x 20cm $60.82 CAD
    16cm x 16 cm $40.27 CAD
    13cm x 9cm $10.03 CAD
    8.6cm x 7cm $10.03 CAD

    SYPRO Ruby
    26cm x 20cm $146.34 CAD
    16cm x 16cm $73.67 CAD
    13cm x 9cm $18.32 CAD
    8.6cm x 7cm $18.32 CAD

    Silver
    26cm x 20cm $11.33 CAD
    16cm x 16cm $5.67 CAD
    13cm x 9cm $2.83 CAD
    8.6cm x 7cm $1.42 CAD

    ProQ Diamond
    26cm x 20cm $195.45 CAD
    16cm x 16cm $140.31 CAD
    13cm x 9cm $42.72 CAD
    8.6cm x 7cm $16.33 CAD

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    Genomic DNA extraction
    $31.45 USD
    per Plate

    Fly Genomic DNA extraction using robotics:
    Submit 5 Drosophila flies/well in 96-well plates to get genomic DNA suitable for PCR genotyping.
    $35 CAD per plate (96 samples per plate = $0.36 CAD per sample)

    Fly Genomic DNA extraction using robotics:
    Submit 5 Drosophila flies/well in 96-well plates to get genomic DNA suitable for PCR genotyping.
    $35 CAD per plate (96 samples per plate = $0.36 CAD per sample)

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    Immunization
    Price on request

    Price estimate:
    $1216 CAD (2 rabbits)
    $788 CAD (4 rats)

    Price estimate:
    $1216 CAD (2 rabbits)
    $788 CAD (4 rats)

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    Live cell imaging
    $48.45 USD
    per Hour

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.

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    Molecular cloning
    $726.81 USD
    per Sample
    Please contact Cell Imaging and Analysis Network (CIAN) for more information regarding this service.
    Multidimensional Imaging
    $48.45 USD
    per Hour

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.

    Z-stack (3D)
    Time-lapse
    Multi-color allow up to 6-dimension imaging

    $50 CAD per hour usage, additional $60 CAD per hour for technical assistance.

    Z-stack (3D)
    Time-lapse
    Multi-color allow up to 6-dimension imaging

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    Off-gel IEF
    Off-gel isoelectric focusing
    $11.57 USD
    per Gel

    Users operated (training ensured by the CIAN)
    24 cm $17.39 CAD
    13 cm $14.18 CAD
    11 cm $14.18 CAD
    7 cm $11.94 CAD

    Users operated (training ensured by the CIAN)
    24 cm $17.39 CAD
    13 cm $14.18 CAD
    11 cm $14.18 CAD
    7 cm $11.94 CAD

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    Plasmid DNA extraction
    $50.08 USD
    per Plate

    $55 CAD per plate (96 samples per plate = $0.57 CAD per sample)
    Using robotics:
    Submit E. coli clones on agar plates or as liquid cultures in tubes or 96-deep well plates for plasmid DNA miniprep.

    $55 CAD per plate (96 samples per plate = $0.57 CAD per sample)
    Using robotics:
    Submit E. coli clones on agar plates or as liquid cultures in tubes or 96-deep well plates for plasmid DNA miniprep.

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    Polyclonal antibody production
    Price on request

    Includes cloning, expression optimization, antigen expression and purification, immunization, serum testing and antibody purification
    - immunization of 4 rats $3288 CAD
    - immunization of 2 rabbits $3716 CAD

    Includes cloning, expression optimization, antigen expression and purification, immunization, serum testing and antibody purification
    - immunization of 4 rats $3288 CAD
    - immunization of 2 rabbits $3716 CAD

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    Protein Expression Optimization
    $290.72 USD
    per Sample

    Add $100 CAD if sequence is to be verified

    Add $100 CAD if sequence is to be verified

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    Recombinant protein production
    $726.81 USD
    per Sample

    His- or GST-tagged fusion in E. coli
    Subcloning into expression vector tagging available
    Expression optimization
    Antigen expression and purification

    His- or GST-tagged fusion in E. coli
    Subcloning into expression vector tagging available
    Expression optimization
    Antigen expression and purification

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    Robotics
    $4.84 USD
    per Hour

    Sagian Core System

    Custom Automated Assay Development
    High-Throughput Screening
    We design, program and validate methods that fit your needs.

    Examples of custom methods:
    - siRNA screen with fluorimetric readout
    - yeast SGA screens
    - ELISA
    - Cherry picking for secondary screens
    -... Show more »

    Sagian Core System

    Custom Automated Assay Development
    High-Throughput Screening
    We design, program and validate methods that fit your needs.

    Examples of custom methods:
    - siRNA screen with fluorimetric readout
    - yeast SGA screens
    - ELISA
    - Cherry picking for secondary screens
    - PCR-based genotyping
    - Library re-array (E. coli library for an RNAi screen in C. elegans)
    - Re-format of a S. cerevisiae collection from 96-well plates to individual vials

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    SDS-PAGE
    Sodium dodecyl sulfate polyacrylamide gel electrophoresis
    $6.10 USD
    per Gel

    Users operated (training ensured by the CIAN)
    Hand cast gel and run:
    26cm x 20cm $17.60 CAD
    16cm x 16cm $12.83 CAD
    8.6cm x 7cm $6.30 CAD

    precast run:
    26cm x 20cm $13.10 CAD
    16cm x 16cm$10.87 CAD
    13cm x 9cm $25.21 CAD

    Users operated (training ensured by the CIAN)
    Hand cast gel and run:
    26cm x 20cm $17.60 CAD
    16cm x 16cm $12.83 CAD
    8.6cm x 7cm $6.30 CAD

    precast run:
    26cm x 20cm $13.10 CAD
    16cm x 16cm$10.87 CAD
    13cm x 9cm $25.21 CAD

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    Serum testing
    $387.63 USD
    per Sample

    For antibody production

    For antibody production

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    Synthetic genetic array (SGA) screen
    Price on request

    Using robotics

    Using robotics

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    qPCR
    Quantitative PCR
    Price on request

    Users operated (training ensured by the CIAN)
    Operates on a bring your own reagent principle

    Users operated (training ensured by the CIAN)
    Operates on a bring your own reagent principle

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