Pjdyzl9stdxdrqnwsi4a sanfordburnham

Cancer Metabolism Facility

La Jolla, California, US

The scientific focus of the Sanford Burnham Prebys Cancer Metabolism Core is to investigate the role of metabolism in cancer on both the cellular and organismal level, combining in vitro and in vivo analysis. To accomplish this, we integrate a number of instruments and methodologies to build an overall model of cellular metabolism. These approaches range from the relatively simple (such as rapidly measuring basic metabolic substrates from media or plasma) to rather complex (tracing the metabolism of stable isotope-labeled substrates in vitro or in vivo).

Recent Publications:

Ratnikov, B., Aza-Blanc, P., Ronai, Z.A., Smith, J.W., Osterman, A.L., and Scott, D.A. (2015) Glutamate and asparagine cataplerosis underlie glutamine addiction in melanoma. Oncotarget 6, 7379-7389.

LaGory E.L., Wu, C., Taniguchi, C.M., Ding, C.K., Chi, J.T., von Eyben, R., Scott, D.A., Richardson, A.D., and Giaccia, A.J. (2015)... Show more »

The scientific focus of the Sanford Burnham Prebys Cancer Metabolism Core is to investigate the role of metabolism in cancer on both the cellular and organismal level, combining in vitro and in vivo analysis. To accomplish this, we integrate a number of instruments and methodologies to build an overall model of cellular metabolism. These approaches range from the relatively simple (such as rapidly measuring basic metabolic substrates from media or plasma) to rather complex (tracing the metabolism of stable isotope-labeled substrates in vitro or in vivo).

Recent Publications:

Ratnikov, B., Aza-Blanc, P., Ronai, Z.A., Smith, J.W., Osterman, A.L., and Scott, D.A. (2015) Glutamate and asparagine cataplerosis underlie glutamine addiction in melanoma. Oncotarget 6, 7379-7389.

LaGory E.L., Wu, C., Taniguchi, C.M., Ding, C.K., Chi, J.T., von Eyben, R., Scott, D.A., Richardson, A.D., and Giaccia, A.J. (2015) Suppression of PGC-1α Is Critical for Reprogramming Oxidative Metabolism in Renal Cell Carcinoma. Cell Rep. 12, 116-127.

Avellaneda Matteo, D., Grunseth, A.J., Gonzalez, E.R., Anselmo, S.L., Kennedy, M.A., Moman P., Scott, D.A., Hoang, A., Sohl, C.D. (2017) Molecular mechanisms of isocitrate dehydrogenase 1(IDH1) mutations identified in tumors: The role of size and hydrophobicity at residue 132 on catalytic efficiency. J Biol Chem. 292,7971-7983.

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Metabolite Profiling
Starting at $81.00 per plate

The YSI 2950 metabolite analyzer is able to measure glucose, glutamine, lactate, and glutamate in media or other liquid samples in 2-3 minutes for all 4 metabolites. It handles 96-well plates with a minimum volume of 100 μl per well. A full 96-well plate may be analyzed in 3-4 hours of hands-free automation. Price is per plate for... Show more »

The YSI 2950 metabolite analyzer is able to measure glucose, glutamine, lactate, and glutamate in media or other liquid samples in 2-3 minutes for all 4 metabolites. It handles 96-well plates with a minimum volume of 100 μl per well. A full 96-well plate may be analyzed in 3-4 hours of hands-free automation. Price is per plate for non-profits. Turn-around usually 3-10 days from receipt.

Pricing*:
Pricing per sample: $54 (external non-profit) / $105.20 (external for-profit)
YSI analysis (reagents & use) per minute: $0.27 (external non-profit) / $0.53 (external for-profit)
* Rates shown do not reflect internal subsidies, such as those for Sanford-Burnham Cancer Center members.

CMR167

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Cellular Bioenergetics
Starting at $270.00 per run

Measurement of cellular respiration and glycolysis using a Seahorse XFp is available for San Diego users.

Pricing:
Per XFp plate: $270 (external non-profit) / $526 (external for-profit)
Additional information:
The Seahorse XFp can simultaneously measure the two major energy producing pathways of live cells (mitochondrial... Show more »

Measurement of cellular respiration and glycolysis using a Seahorse XFp is available for San Diego users.

Pricing:
Per XFp plate: $270 (external non-profit) / $526 (external for-profit)
Additional information:
The Seahorse XFp can simultaneously measure the two major energy producing pathways of live cells (mitochondrial respiration and glycolysis) in a microplate in real-time. This fast and sensitive measurement of cellular bioenergetics is label free, enabling time-resolved analysis and the reuse of the cells.

The instrument determines the rate of glycolysis and respiration of adherent cells in a 6-well format. Glycolysis is observed via the acidification of the tissue culture media. Respiration is observed by measuring total oxygen consumption. With proper experimental design, the rates of basal respiration, ATP production, proton leak, maximal respiration, spare respiratory capacity and non-mitochondrial oxygen consumption may be determined.

Note: this service is only available in the La Jolla/ San Diego area (customer cultures cells, we provide plates and reagents).

CMR166

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Agilent Seahorse XF Analyzer
GC-MS
Gas Chromatography Coupled Mass Spectrometry
Starting at $54.00 per sample

GC/MS-based analysis of metabolites including amino acids, keto acids, sugars, fatty acids, short-chain fatty acids and cholesterol.

Pricing:
Pricing per sample: $54 (external non-profit) / $105.20 (external for-profit)
Pricing reflects determination of 13C-labeling OR metabolite quantification; for both services, prices are... Show more »

GC/MS-based analysis of metabolites including amino acids, keto acids, sugars, fatty acids, short-chain fatty acids and cholesterol.

Pricing:
Pricing per sample: $54 (external non-profit) / $105.20 (external for-profit)
Pricing reflects determination of 13C-labeling OR metabolite quantification; for both services, prices are $108/ $110.40. Additional charges may apply for sample preparation.

Additional information:
The Shimadzu QP2010plus gas chromatograph-mass spectrometer is used for metabolite quantification and metabolic flux analysis including determination of stable isotope labeling rates of various intra- and extracellular metabolites. A panel of 35-40 polar metabolites typically detected in cells includes amino acids and TCA cycle and glycolysis metabolites. Fatty acid abundance and 13C labeling are also readily determined. Measuring both the abundances of these metabolites and the rate of stable isotope labeling enables determination of the relative activity of a number of metabolic pathways involved in tumor progression, including glycolysis, the pentose phosphate pathway, the TCA cycle, de novo fatty acid biosynthesis and amino acid biosynthesis. In addition, the metabolism or exchange of other isotope labeled metabolites can be tracked as needed.

CMR164

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Cellular Health & Metabolism Assays
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Mass Spectrometry
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Other Mass Spectrometry Methods
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Metabolomics
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Biology
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Cell-Based Assays
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