Brooklyn Bio Inc. is your friendly neighborhood biotech startup, founded in New York City. The company specializes in molecular techniques to generate and test genetic constructs. Our areas of expertise include bacterial, yeast, and plant genetics. We want to work with you to take the headache out of cloning by designing and/or executing your ideal cloning strategies. Our goal is to maximize efficiency of your experiments by providing reliable testing and tools.
We can work with your team to develop the best work flow for your project. We can recommend experimental procedures, starting material sources, and alternate plans to ensure your success. Our experts in plant genetics, microbiology, molecular biology, and data visualization can advise and offer project management solutions.
Genomic DNA purification; gene amplification by PCR; recombinant cloning with restriction enzymes, CRISPR, or Gibson assemblies. Overexpression, site-directed mutagenesis, or genetic knockouts, using bacterial, yeast, or plant systems. We can provide purified DNA, plasmids, or genetically-modified organisms.
Design of knock-out, knock-in, fusion protein genes systems. Site-directed mutagenesis or biochemical pathway manipulation
Construct design; cloning as required from genomic DNA or RNA; protein overexpression; genetic knock-out or knock-in systems; plant, fungi, yeast, and bacterial systems
Construct design; cloning as required from genomic DNA or RNA; protein overexpression and purification; genetic knock-out or knock-in systems; plant, fungi, yeast, and bacterial systems
Recombinant DNA manipulation; restriction enzyme analysis; DNA purification; DNA sequencing. Quantitative PCR; RNA purification; RNA reverse transcription; cDNA generation and cloning.
Site-directed mutagenesis; recombinant DNA manipulation; restriction enzyme analysis; DNA purification; DNA sequencing
Protein overexpression; protein purification; protein concentration; antigen purification; apoptosis assays; ELISA (enzyme-linked immunosorbant assay); Western blot
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