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Brains On-Line - A Charles River Company

South San Francisco, California, US

Our mission at Brains On-Line is to provide preclinical research services to facilitate the development of new drugs that target the central nervous system.

Brains On-Line benefits from its intimate connections with academia, as well as with several associated contract research organizations (CRO). Using our expertise, we aim to give full insight in the possibilities of your compound.

Brains On-Line - A Charles River Company has not listed any services.

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Animal Behavior Studies
Price on request

Anxiety/Depression
Forced Swim Test
Tail Suspension Test
Light/Dark Box
Cognition
-Novel Object Recognition (Natural Forgetting, Scopolamine -Induced Deficits, Phencyclidine Induced Deficits)
-Contextual Fear Conditioning
Motor Activity/Coordination
-Home Cage Locomotor Activity
-Open Field Test
-Rotorod
-Grip... Show more »

Anxiety/Depression
Forced Swim Test
Tail Suspension Test
Light/Dark Box
Cognition
-Novel Object Recognition (Natural Forgetting, Scopolamine -Induced Deficits, Phencyclidine Induced Deficits)
-Contextual Fear Conditioning
Motor Activity/Coordination
-Home Cage Locomotor Activity
-Open Field Test
-Rotorod
-Grip Strength
Psychosis/Mania
-Apomorphine Rearing/Climbing
-Psychostimulant Induced Locomotion
All behavior studies are carried out in mice or rats in our state of the art U.S. facility under review of an Institutional Animal Care and Use Committee. In addition to the array of pharmacologically validated assays, we strive to develop of new tests (in house or in collaboration with the sponsor). Given our expertise in microdialysis, all of our behavioral studies can be coupled to in vivo neurotransmitter monitoring via traditional microdialysis or with the use of biosensors.

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Animal Surgery
Price on request

-Jugular vein cannulation
-Femoral vein cannulation
-Probe/sensor implantation brain
-Probe implantation cerebral spinal fluid
-Probe implantation bone
-Probe/sensor implantation in spinal cord
-All types of stereotaxic surgery
-Osmotic minipump implantation
-Catheter placement

We work with a number of generally... Show more »

-Jugular vein cannulation
-Femoral vein cannulation
-Probe/sensor implantation brain
-Probe implantation cerebral spinal fluid
-Probe implantation bone
-Probe/sensor implantation in spinal cord
-All types of stereotaxic surgery
-Osmotic minipump implantation
-Catheter placement

We work with a number of generally available animal models, please contact us with requests.

We frequently ship or receive samples by courier service, sealed and packaged on dry-ice for proper conservation. Intercontinental expeditions are usually delivered within three to four days. Brains On-Line holds the required permits for shipping biological samples into and out of The Netherlands.

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Animal Microsensor Studies
Price on request

Microsensoring has a higher spatial (10-25 μm diameter) and temporal resolution (second-scale recording of neurotransmitters) than microdialysis. Therefore, it can provide more spatial-temporal resolution measurements and capture the fast change neurotransmitter levels, glucose or other physiological compounds.

In our lab we... Show more »

Microsensoring has a higher spatial (10-25 μm diameter) and temporal resolution (second-scale recording of neurotransmitters) than microdialysis. Therefore, it can provide more spatial-temporal resolution measurements and capture the fast change neurotransmitter levels, glucose or other physiological compounds.

In our lab we routinely monitor brain glutamate and periphery glucose levels in both anesthetized and freely moving animals.

Currently we have sensors available for the monitoring of
-Lactic acid
-Glutamate
-Glucose
-NO
-H2O2
-Acetylcholine
-Choline

Sensors can be applied in
-Brain
-Spinal cord
-Subcutanous

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Animal Blood Collection
Price on request

Blood sampling is performed using automated Dilab AccuSamplers®
-Higher degree of precision and repeatability
-Sampling over prolonged periods of time
-Robust set-up for overnight operation
-Freely-moving and stress free conditions

Blood sampling is performed using automated Dilab AccuSamplers®
-Higher degree of precision and repeatability
-Sampling over prolonged periods of time
-Robust set-up for overnight operation
-Freely-moving and stress free conditions

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Cognition and Behavior Animal Models
Price on request

Sub-chronic Phencyclidine

Sub-chronic Phencyclidine

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Huntington's Disease Animal Models
Price on request

Quinolinic Acid Huntington's Model

Quinolinic Acid Huntington's Model

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Immunocytochemistry
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ICC is particularly suitable to answer questions related to the localization of signal transduction markers. ICC is routinely performed on brain sections that can best be prepared by means of frozen sectioning. The availability of primary antibodies or radioligands directed against neurotransmitters, cell surface receptors and... Show more »

ICC is particularly suitable to answer questions related to the localization of signal transduction markers. ICC is routinely performed on brain sections that can best be prepared by means of frozen sectioning. The availability of primary antibodies or radioligands directed against neurotransmitters, cell surface receptors and intracellular receptors, intracellular proteins, peptides etc. these days is nearly unlimited. We have experience with these techniques, with particular interest in receptor systems, G-proteins, kinases and phophatases, neuropeptides, neurotransmitters and neurotransmitter-specific enzymes, cytoskeletal proteins; to mention only a few groups of biomarkers that are being studied.

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Immunohistochemistry (IHC)
Price on request

ICC is particularly suitable to answer questions related to the localization of signal transduction markers. ICC is routinely performed on brain sections that can best be prepared by means of frozen sectioning. The availability of primary antibodies or radioligands directed against neurotransmitters, cell surface receptors and... Show more »

ICC is particularly suitable to answer questions related to the localization of signal transduction markers. ICC is routinely performed on brain sections that can best be prepared by means of frozen sectioning. The availability of primary antibodies or radioligands directed against neurotransmitters, cell surface receptors and intracellular receptors, intracellular proteins, peptides etc. these days is nearly unlimited. We have experience with these techniques, with particular interest in receptor systems, G-proteins, kinases and phophatases, neuropeptides, neurotransmitters and neurotransmitter-specific enzymes, cytoskeletal proteins; to mention only a few groups of biomarkers that are being studied.

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Animal Microdialysis
Price on request

Microdialysis a widely use method to monitor concentrations distribution and elimination of neurotransmitters in the brain of freely moving animals. Pharmacodynamics describe the time-dependent response of the neurotransmitters to pharmacological or behavioural challenges.
Local release of the following neurotransmitters can be... Show more »

Microdialysis a widely use method to monitor concentrations distribution and elimination of neurotransmitters in the brain of freely moving animals. Pharmacodynamics describe the time-dependent response of the neurotransmitters to pharmacological or behavioural challenges.
Local release of the following neurotransmitters can be monitored:
-Acetylcholine
-Dopamine
-Gamma-amino butyric acid (GABA)
-Histamine / tele-Methyl-Histamine
-Norepinephrine
-Serotonin
-Glutamate
-Glycine
-D- and L- serine
-DOPAC, 5-HIAA, HVA and MHPG

But also these other factors that influence neuronal activity can be monitored:
-Choline
-Adenosine, cAMP and cGMP
-Glucose / lactate / pyruvate
-Glycine
-Taurine
-Kynurenine pathway metabolites
-Ethanol-amides

The following administration routes are available:
-Locally (i.e. via the microdialysis probe, sometimes referred to as 'reverse or -retro dialysis'; or iontophoretically)
-Systemically (i.p., s.c. or i.v. through a pemanent catheter)
-Both routes combined

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Parkinsons Disease Animal Models
Price on request

6-hydroxydopamine Parkinson's Model

6-hydroxydopamine Parkinson's Model

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Preclinical PK/PD Studies
in vivo Pharmacokinetics/Pharmacodynamics
Price on request

The MetaQuant microdialysis probe is used within Brains On-Line to make ultra slow microdialysis a medium throughput technique. A unique characteristic of MetaQuant microdialysis is that information can be obtained about the unbound concentrations of a compound in the extracellular spaces in the body.

Brains On-Line routinely... Show more »

The MetaQuant microdialysis probe is used within Brains On-Line to make ultra slow microdialysis a medium throughput technique. A unique characteristic of MetaQuant microdialysis is that information can be obtained about the unbound concentrations of a compound in the extracellular spaces in the body.

Brains On-Line routinely carries out studies that evaluate PK-PD relationships of CNS targeted drugs which are affected by important physiological processed, including:

-Plasma (protein) binding
-Blood-brain barrier transport
-Brain tissue binding
-Receptor kinetics

The MetaQuant offers novel quantitative microdialysis.
-Nearly 100% recovery due to (very) low microdialysis flow
-Consistent data with little variation
-Fewer animals needed (66% reduction compared to Dynamic No Net Flux)
-Faster turnover of studies because there is no need for extensive validation and screening in pilot experiments
-No intrinsic pharmacological effects from compounds added to the perfusate
-Easy combination with jugular vein/femoral vein catheters to assess brain and systemic pharmacokinetics and /or effects within the same animal

At Brains On-Line we have extensive experience with combining microdialysis (regular or MetaQuant probes) and catheters into the jugular vein and/or femoral vein. Allowing simultaneous monitoring of brain and systemic parameters within the same subject.

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In vivo Electrophysiology
Price on request

At Brains On-Line we carry out IN VIVO single-cell recordings to evaluate the effects of different pharmaceuticals on:
-Neuronal firing rate
-Number of neurons per trackt
-Long Term Potentiation

Neurons are identified by
-Their location
-Waveform
-Firing rate
-Response to known (ant)agonists

On this basis we routinely... Show more »

At Brains On-Line we carry out IN VIVO single-cell recordings to evaluate the effects of different pharmaceuticals on:
-Neuronal firing rate
-Number of neurons per trackt
-Long Term Potentiation

Neurons are identified by
-Their location
-Waveform
-Firing rate
-Response to known (ant)agonists

On this basis we routinely study
-Serotonergic neurons (DRN/MRN)
-Adrenergic neurons (LC)
-Dopaminergic neurons (VTA/SN)
-Histaminergic neurons (TMN)
-Glutamatergic neurons

Animals are fixed in position and kept under propofol anesthesia and at constant body temperature; drugs are systemically administered through a lateral tail vein, signal is recorded and analysed with the SPIKE2 software package.

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Western Blot
Price on request

Blotting of proteins or peptides, commonly named 'Western blotting', is the method of choice whenever information is needed on protein identification and protein quantitation.

Western blotting can be used a biochemical quantitation method and a topographical localization technique. Western blotting can be performed both... Show more »

Blotting of proteins or peptides, commonly named 'Western blotting', is the method of choice whenever information is needed on protein identification and protein quantitation.

Western blotting can be used a biochemical quantitation method and a topographical localization technique. Western blotting can be performed both on freshly prepared brain-slices and on homogenized brain-tissue. After tissue sample homogenization and they can be subjected to a number of separation treatments to select, for example membrane bound proteins or cytoplasmic proteins. After poly acrylamide gel electroforeses and transferal to a membrane antibodies can be used to detect and qunatify a protein/receptor of interest.

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Veterinary Laboratory Services
Price on request
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ADME/DMPK Studies
Drug Metabolism and Pharmacokinetics
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Veterinary Research & Diagnostics
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Animal Learning, Memory, and Behavior Tests
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Animal Models of Disease
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Pharmacology & Toxicology
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Protein Services
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Immunostaining
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Protein Expression Visualization
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Animal Models and Studies
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Animal Model in vivo Analyses
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Animal Cognition & Behavior Tests
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CNS/Neurology Animal Models
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Biochemistry & Molecular Biology
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Cognitive, Behavioral, and Psychiatric Animal Models
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Biology
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Animal Physiology Analyses
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