Io3glptnscomt299opmp bps

BPS Bioscience

San Diego, CA, US

Our mission is to provide the highest quality life science products and services in a timely manner to help our customers accelerate drug discovery research and development for the treatment of human diseases.

Our scientists are experts in the design, expression, purification, and characterization of active recombinant enzymes and enzyme complexes. We are also highly skilled in developing recombinant cell lines and all aspects of biochemical and cell-based assay development. A separate group of Ph.D.-level scientists is dedicated to offering drug discovery services for targeting epigenetics, immune checkpoint, metabolism, and cell signaling pathways.

Innovation is a key part of BPS’s core focus. BPS was the first company to offer a complete suite of HDAC, PARP, and PDE enzymes, and continues to offer the broadest selection of bromodomains, methyltransferases, demethylases, methyl-lysine readers, and other... Show more »

Our mission is to provide the highest quality life science products and services in a timely manner to help our customers accelerate drug discovery research and development for the treatment of human diseases.

Our scientists are experts in the design, expression, purification, and characterization of active recombinant enzymes and enzyme complexes. We are also highly skilled in developing recombinant cell lines and all aspects of biochemical and cell-based assay development. A separate group of Ph.D.-level scientists is dedicated to offering drug discovery services for targeting epigenetics, immune checkpoint, metabolism, and cell signaling pathways.

Innovation is a key part of BPS’s core focus. BPS was the first company to offer a complete suite of HDAC, PARP, and PDE enzymes, and continues to offer the broadest selection of bromodomains, methyltransferases, demethylases, methyl-lysine readers, and other epigenetic tools, many of which are not commercially available elsewhere. We are the first and only company offering screening services for DNMTs, tankyrase and PARPs, demethylases, pyruvate kinases, and many of our methyltransferases. Continued innovation has allowed us to expand our areas of expertise to meet research demands.

BPS maintains a customer-oriented approach; we listen to our customers and customize our services to meet their specific research requirements. With any request, we aim to support our customers in reaching their research goals. By focusing on our core competencies, we can quickly provide personalized service and targeted results. BPS has provided products and services to pharmaceutical companies, biotechnology companies, and academic institutes in over 45 countries worldwide.

« Show less

BPS Bioscience has not listed any services.

Stable Cell Line Generation
Price on request

Reduce your workload by letting us create or transform high quality, stable cell lines appropriate for your research. Learn more about our Cell Line Development Services.

Mammalian Stable Cell Line Development

BPS provides a custom service to generate stable cell lines that constantly express proteins of interest at high,... Show more »

Reduce your workload by letting us create or transform high quality, stable cell lines appropriate for your research. Learn more about our Cell Line Development Services.

Mammalian Stable Cell Line Development

BPS provides a custom service to generate stable cell lines that constantly express proteins of interest at high, homogeneous levels. The gene of interest will be cloned into the desired mammalian expression vector. The parent cell line will then be tested for its sensitivity to selection antibiotics and optimized for transfection conditions.

The stable cell line is generated by the transfection of the expression construct into the parent cell line with subsequent selection with antibiotics. The individual stable clones will be expanded and screened by Western Blot for optimal expression of the protein of interest. Quality control testing will be performed to ensure that the final stable cell lines meet the requirements of the customers.

If you would like to learn more about our mammalian expression service, please contact us.

« Show less
Custom Cell-Based Assays
Price on request

BPS Bioscience has the capability to develop custom assays designed to meet your specific research needs. We will work with you to ensure that the development of your custom assay is easy and simple, enabling you to spend more time focusing on key elements of your research.

BPS Bioscience has the capability to develop custom assays designed to meet your specific research needs. We will work with you to ensure that the development of your custom assay is easy and simple, enabling you to spend more time focusing on key elements of your research.

« Show less
Monoclonal Antibody (mAb) Production
Price on request

BPS provides mAb transient expression service by using serum free HEK293 suspension system.

Pilot expression and purification will be evaluated and optimized. Using the results of these experiments, mAb expression will be scaled up and purified by affinity chromatography. You will receive the purified antibody and a complete... Show more »

BPS provides mAb transient expression service by using serum free HEK293 suspension system.

Pilot expression and purification will be evaluated and optimized. Using the results of these experiments, mAb expression will be scaled up and purified by affinity chromatography. You will receive the purified antibody and a complete project report with the SDS-PAGE/Western blot analysis that documents the antibody production.

« Show less
Bacterial Protein Expression
Price on request

Subcloning/PCR Cloning

BPS will clone your insert into an expression vector. Either a standard subcloning or a PCR cloning will be used to transfer the gene of interest into the expression vector. Clones will be screened for the correct restriction fragments and sequenced.

Expression Screening

The expression vector... Show more »

Subcloning/PCR Cloning

BPS will clone your insert into an expression vector. Either a standard subcloning or a PCR cloning will be used to transfer the gene of interest into the expression vector. Clones will be screened for the correct restriction fragments and sequenced.

Expression Screening

The expression vector containing the gene of interest will be transformed into an expression strain of E. coli for protein production. Three to five individual clones will be screened in a small-scale induction experiment and analyzed by SDS/PAGE for production of a protein of the expected size. If desired, a Western blot can be carried out to verify immunoreactivity with your antisera, or with an anti-affinity tag antisera.

Optimization of Expression

The expression of the recombinant protein can be optimized by doing a time course of induction, altering induction temperature or screening growth media. For each test, the recombinant protein will be analyzed by SDS-PAGE and/or Western blot to determine optimal conditions for protein production.

Protein Characterization

A small-scale culture (10-100 ml) from one isolate of the strain producing the recombinant protein will be induced and the expressed protein will be characterized for cellular location, solubility, and accessibility of purification tags. In addition, the yield per unit volume will be estimated. Using the results of these experiments, a strategy for producing and purifying the protein will be made.

Protein Production

Using shake flasks the strain expressing the recombinant protein can be scaled up between one and several liter volumes. You will receive the cell pellets and a complete project report with the SDS-PAGE and/or Western blot analysis that document protein production.

Protein Purification

BPS will purify your recombinant protein(s). We offer a variety of services from inclusion body preparation to affinity column purification.

If you would like to learn more about our E.coli expression service, please contact us.

« Show less
Baculovirus Protein Expression
Price on request

Subcloning/PCR Cloning

BPS will clone your insert into a baculovirus transfer vector. Either a standard subcloning or a PCR cloning will be used to transfer the gene of interest into the vector. Clones will be screened for the correct restriction fragments and sequenced, if necessary.

*Recombinant Virus... Show more »

Subcloning/PCR Cloning

BPS will clone your insert into a baculovirus transfer vector. Either a standard subcloning or a PCR cloning will be used to transfer the gene of interest into the vector. Clones will be screened for the correct restriction fragments and sequenced, if necessary.

Recombinant Virus Production

Using linearized DNA from Autographa californica nuclear polyhedrosis virus (AcMNPV), BPS will cotransfect a baculovirus transfer vector into Sf9 cells. The low –titer recombinant viruses will be amplified 2-3 times to generate high-titer virus stocks.

Optimization of Expression

Recombinant protein expression will be optimized in one or more cell lines by doing a time-course study of infection, varying the multiplicity of infection (MOI). At each time point, the expressed protein will be analyzed by SDS-PAGE and/or Western blot. The results of these experiments will be used to determine the optimal conditions for protein production.

Protein Characterization

Recombinant protein from a small-scale culture will be characterized for cellular location, solubility, and accessibility of purification tags. In addition, the yield per unit volume will be estimated. Using the results of these experiments, a strategy for producing and purifying the protein will be made.

Protein Production

Production of the recombinant protein can be scaled up between one and several liter volumes. You will receive the cell pellets or conditioned media and a complete project report with the SDS-PAGE and/or Western blot analysis that document protein production.

Protein Purification

BPS will purify your recombinant proteins using an affinity column. Large scale purification is also available.

If you would like to learn more about our baculovirus expression service, please contact us.

« Show less
Protein Expression
Price on request

Subcloning/PCR Cloning

BPS will clone your insert into the desired mammalian expression vector. Either standard subcloning or PCR cloning will be used to transfer the gene of interest into the expression vector. Clones will then be screened for the correct restriction fragments and sequenced.

*Optimization of Expression... Show more »

Subcloning/PCR Cloning

BPS will clone your insert into the desired mammalian expression vector. Either standard subcloning or PCR cloning will be used to transfer the gene of interest into the expression vector. Clones will then be screened for the correct restriction fragments and sequenced.

Optimization of Expression and Protein Characterization

The mammalian expression vector will be transiently transfected into suspension 293 cells grown in a defined, serum-free medium. Recombinant protein production will be optimized in a small-scale culture by doing a time course of expression. For each test, the recombinant protein will be analyzed by SDS-PAGE and/or Western blot. In addition, the yield per unit volume will be estimated. Using the results of these experiments, a strategy for producing and purifying the protein will be made.

Protein Production

Production of the recombinant mammalian-expressed protein can be scaled up to 1 liter. You will receive the cell pellets or conditioned media and a complete project report with the SDS-PAGE/Western blot analysis that documents the protein production.

Protein purification

BPS will purify your recombinant proteins using an affinity column. Large scale purification is also available.

« Show less
Cell Proliferation Assays
Price on request

BPS currently provides tumor cell proliferation assays that can determine the IC50 values of your compounds of interest in whole cell assays. BPS offers over 20 different normal and tumor cell lines for cell screening and proliferation assays.

BPS currently provides tumor cell proliferation assays that can determine the IC50 values of your compounds of interest in whole cell assays. BPS offers over 20 different normal and tumor cell lines for cell screening and proliferation assays.

« Show less
Tumor cells
Phosphodiesterase (PDE) Assays
Price on request

BPS Bioscience now provides services to determine IC50’s or screen PDE inhibitors using our cell-based assays. Our PDE cell-based assays include: PDE1B, PDE4D, PDE7A and other PDEs of your choice.

BPS Bioscience now provides services to determine IC50’s or screen PDE inhibitors using our cell-based assays. Our PDE cell-based assays include: PDE1B, PDE4D, PDE7A and other PDEs of your choice.

« Show less
Immunotherapy Screening
Price on request

BPS Bioscience now provides services to determine EC50’s or screen inhibitors of Immunotherapy proteins using our cell-based assays! We can perform assays in HeLa, HEK293, or other cell lines of your choice.

BPS Bioscience now provides services to determine EC50’s or screen inhibitors of Immunotherapy proteins using our cell-based assays! We can perform assays in HeLa, HEK293, or other cell lines of your choice.

« Show less
Histone Deacetylase (HDAC) Assays
Price on request

BPS Bioscience now provides services to determine IC50’s or screen inhibitors of HDACs using our cell-based assays! We can perform assays in tumor cell lines such as A549, PC-12, or other cell lines of your choice.

BPS Bioscience now provides services to determine IC50’s or screen inhibitors of HDACs using our cell-based assays! We can perform assays in tumor cell lines such as A549, PC-12, or other cell lines of your choice.

« Show less
Signaling Pathway Analysis
Price on request

BPS has developed a number of recombinant reporter cell lines that can be used to screen inhibitors and/or activators of various cell pathways. If you are developing inhibitors specific to one of our pathway reporters, these cell-based systems offer a more complex setting than cell-free systems. This screening service is also a... Show more »

BPS has developed a number of recombinant reporter cell lines that can be used to screen inhibitors and/or activators of various cell pathways. If you are developing inhibitors specific to one of our pathway reporters, these cell-based systems offer a more complex setting than cell-free systems. This screening service is also a great tool that can be used to tease out the specific pathway that a particular compound is targeting; simply send us the compound and we can screen against our entire portfolio or a select few pathways. For a list of the pathways offered, click here. With any screen, we provide rapid, dependable profiling of compounds against the requested pathway-specific cell lines by EC50 or IC50 determination. Contact us for more details.

« Show less
Enzyme Assays
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Cell-Based Assays
Price on request
Request a quote for more information about this service.
Biochemical Assays
Price on request
Request a quote for more information about this service.
Hydrolase Assays
Price on request
Request a quote for more information about this service.
Cell and Tissue Culture
Price on request
Request a quote for more information about this service.
Antibody Services
Price on request
Request a quote for more information about this service.
Specialized Cell-Based Assays
Price on request
Request a quote for more information about this service.
Cell Viability & Proliferation Assays
Price on request
Request a quote for more information about this service.
Cell Signaling Analyses
Price on request
Request a quote for more information about this service.
Ask A Question Find what you're looking for? If not, you can ask this lab a question:

BPS Bioscience has not received any ratings.

Be the first to endorse BPS Bioscience

Endorse this lab