Boster Biological Technology

Custom Rabbit Polyclonal Antibody starting from $600 

Workflow Overview:

• Antigen Expression: Design & express recombinant antigen
• Immunization: Immunize 2 rabbits
• Purification: Affinity purification and validation
• Deliverables: ELISA test report, 3-5mg of the purified antibody
• Workflow Duration: 3-4 months

Regular Custom Rabbit Polyclonal Antibody Service

Request a quote for your project to start the discussion. Please provide the target sequence/Uniprot ID, applications (IHC, IF, WB), and your other requirements.

Promotion: $600 Custom Rabbit Polyclonal Antibody

Boster Bio offers a special $600 custom polyclonal antibody production package, which includes the recombinant antigen expression service and antibody purification. If your custom antibody project falls into any of the 3 purposes below, you are eligible for the promotion:

1. You work with non-traditional model organisms (e.g. zebrafish, drosophila, frog, yeast...anything other than human and murine).
2. Your research is related to COVID-19.
3. You want to replace a discontinued antibody.

Our 27+ years of experience in antibody design and production will ensure you the best chance of getting a high affinity rabbit polyclonal antibody that works for your application. Since 1993, our team has produced over 10,000 antibodies, so we have full confidence to help you with your custom antibody project.

All you need to provide is (1) your target sequence/Uniprot ID, (2) applications (IHC, IF, WB), and (3) your other requirements, and our lab will get to work.

Our team of experts will provide support for each step of antibody production - from antigen design to final antibody purification. You will be notified when each milestone is achieved. If you have any questions along the way, feel free to send us a message and we’ll make sure you stay informed. Contact us for a quote today!

Custom Rabbit Monoclonal Antibody

Workflow Overview:

• Peptide synthesis: 10mg, >90% purity
• Peptide conjugation: conjugation KLH for immunization, BSA-biotin conjugation for probing and screening
• Immunization: 2 rabbits
• ELISA assay monitoring to select best animal for monoclonal antibody
• Screen with FACS (>a million B cells)
• Supernatant production and secondary screening with ELISA
• Deliverables: Summary report, ELISA data, sequence information of delivered clones, map of the vector, 0.5mg (0.1-1mg) of purified antibody from 2 chosen clones, 100ng of heavy and light chain plasmid DNA for final chosen clone
• Duration: 4-6 months

Custom Mouse Monoclonal Antibody

Workflow Overview:

• Antigen Expression: Design & express recombinant antigen
• Immunization & test-bleed on ELISA: immunize 3 mice
• Fusion & Screening: Harvest splenocytes for hybridoma fusion & Screen for positive clones
• Subcloning: Subclone & isolate stable positive clones
• Purification: Purify and test antibodies
• Deliverables: Final delivery report, 2mg monoclonal antibodies, 2 hybridoma clones, 6-month cell banking service
• Workflow Duration: 5-6 months

Our 27+ years of experience in antibody design and production will ensure you the best chance of getting a high affinity rabbit or mouse monoclonal antibody that works for your application. Since 1993, our team has produced over 10,000 antibodies, so we have full confidence to help you with your custom antibody project.

All you need to provide is your target sequence/Uniprot ID, applications (IHC, IF, WB), and your other requirements, and our lab will get to work.

Our team of experts will provide support for each step of antibody production - from antigen design to final antibody purification. You will be notified when each milestone is achieved. If you have any questions along the way, feel free to send us a message and we’ll make sure you stay informed. Contact us for a quote today!

Singleplex & Multiplex ELISA Testing Service Overview

In a nutshell: You send us your samples, we test them for your analytes of interest and send you the results data.

1. Select Your Assay: Choose from a range of singleplex & multiplex ELISA kits or create a custom multiplex assay.
2. Ship Your Samples: Ship your samples with 3-5 lbs dry ice to our facility and we'll get started.
3. Get Results: We'll deliver detailed data results to you within 2-3 weeks of receiving your samples.

Highlights of the service:

• We can do all sandwich ELISA, EIA, and Phosphorylation ELISA kits.
• We can source positive and negative controls.
• Guaranteed results with high quality assay specifications for sensitivity and reproducibility.
• Our experiences allow us to provide custom consultation to help you decide the configuration based on the nature of each analyte.
• If you need help deciding which analytes are the best markers for your research topic, we can consult on that, too.

Typical ELISA Service Workflow

1. Assay Design Consultation: We discuss the goals of your assays and help you decide on analytes/biomarkers, format, and configuration. We have worked with experts in many subject matters and can provide scientific consultation for our customers in addition to simple outsourcing capacity.
2. Kits/Antibodies Selection: We pick the right assay for you - singleplex or multiplex. Boster carries 1,000+ highly sensitive sandwich ELISA kits and a range of other ELISA kit formats. If you want to work with ELISA kits from other companies, we can procure them for you, or you can send your own ELISA kits to us. If no kit is available, we can develop an assay from antibody pairs. Certain antibody combinations are best for specific assay types. For example, in sandwich ELISAs, one antibody traps the analyte, and the other antibody is used to detect. These pairs are tested for compatibility. If you're interested in testing many analytes simultaneously, you can choose from our multiplex ELISA kits or request a custom multiplex ELISA kit.
3. Pilot Studies: Turnaround time is generally 5-20 days. We test standard samples, matrices, conjugates, etc. in combination to create the prototype. We help you select standards and set aside key controls, etc.
4. Technical Transfer Or Scaling Up: We ramp up the testing scale for you, or we transfer the technology to your laboratory, ensuring that the assay works in your technicians' hands. We can also custom manufacture these kits for you if you want to develop them into a product.

Since 1993, Boster has been manufacturing antibodies and ELISA kits for scientists around the world. Our founder developed Picokine™, a proprietary high sensitivity sandwich ELISA platform, in the early 2000s. Over the past two decades, we have developed 1,000+ highly sensitive Picokine™ ELISA kits which have been cited in over 6,000 publications to the end of 2019 (sources: Google Scholar, Bioz, and CiteAb). Hundreds of ELISA assays are run each week by the ELISA experts at Boster Bio. With its history and scale, Boster is able to provide you a unique combination of service quality, subject expertise, and cost savings when you need an ELISA service to streamline your workflow.

Service Overview

Phase 1: Antibody IHC Optimization Service - Finding the best dilution ratio for a new antibody

• For a new antibody, i.e. antibody that has not been validated with IHC, we start off with the initial optimization service to determine the best epitope retrieval method and primary antibody incubation concentration.

Phase 2: IHC Staining On Tissues Of Interest - Using the antigen retrieval method and optimal concentration

• We validate the antibody on sample sets using the conditions set in phase 1. We can carry out the study automated on Leica Bond automated system or manually if requested. We offer cell pellet arrays (CPA) and tissue microarrays (TMA) that consist of different expression levels of the target of interest as the validation sample. To get the results in the most cost-effective way, we recommend customers to test on these arrays because they can get the answer they want from a single slide (per testing condition).

Since 1993, Boster has been manufacturing antibodies and reagents for pathology/IHC. Our founder came from a medical histology background and IHC has been in the company's gene from day 1. With hundreds of IHC experiments performed each week and almost 3 decades of continuous improvement, Boster's technical team has mastered the art of IHC. By the end of 2019, there were more than 5,000 articles published using IHC results obtained from Boster antibodies (source: Google scholar, Bioz and CiteAb). With our history and size, Boster is able to provide you a unique combination of service quality, subject expertise and cost savings when it comes to IHC service.

Western Blot Made Easy

Source your western blot experiments to us if you...

1. Want to free your lab from running gels and transfers.
2. Are bothered by the thought of buying and testing yet one more antibody.
3. Don't have the time to run all those samples.
4. Want to try out a CRO who can be your long term partner in broadening the bandwidth of your lab/team in a flexible manner.

You need to provide

• Purified protein (>50 ug/sample, concentration: >1 mg/ml) or lysate sample (total protein >500 ug/sample, concentration: >1 mg/ml)
• Primary antibody to detect target protein (or purchased from us)

What will you get?

• A full report with digitized WB exposure data image (films and membranes) for each gel we run for you. Gels can have 10-15 lanes per gel.
• Advanced protein characterization: We also provide free consultation to optimize the WB strategy for you if needed--WB sometimes is not sufficient in characterizing protein of interest from contaminants. We can design custom strategies such as using silver stain and LC-MS to identify contaminants.

Since 1993, Boster has been manufacturing antibodies and reagents for proteomics. With hundreds of western blot experiments performed each week and almost 3 decades of continuous improvement, Boster's technical team has mastered the art of WB. By the end of 2019, there were more than 7,000 articles published using WB results obtained from Boster antibodies (source: Google scholar, Bioz and CiteAb). With our history and size, Boster is able to provide you a unique combination of service quality, subject expertise and cost savings when it comes to WB service.

Let us do the tedious antibody testing for you

Our experts will help you test antibodies for IHC, ICC/IF, WB, ELISA & FCM

We've heard from customers how they've wasted so much time and money on testing antibodies from various companies before finding an antibody (usually from Boster) that works for their samples and experiments. The whole process of purchasing (or requesting trial size antibodies) and testing multiple antibodies can be very time-consuming, expensive, and frustrating.

We want to help take that hassle away from you. Our economy of scale and close relationships with other antibody companies enable us to perform antibody testing more cost effectively and efficiently. Send us the targets and applications you are interested in and we'll handle the rest of the initial discovery process. We will do the antibody procurement and testing, so you can save time and money while shortening the cycle for on-boarding a new antibody.

Workflow Overview

1. Send Us Targets: Send us the targets you're interested in and let us know what application(s) you need the antibodies validated for.
2. We Do The Testing: Boster will procure the antibodies on your behalf and perform the testing to determine which antibodies work as expected.
3. Get Results: You will receive a detailed report from us that presents the antibody validation results to help you decide which antibody to buy.

Boster Bio has 27+ years of experience in manufacturing ELISA kits and antibodies. As a trusted ELISA kit company, we proudly offer 1000+ ELISA kits in sandwich ELISA format, covering human, mouse, rat, and various other mammalian species.

Boster's enzyme-linked immunosorbent assays go through painstaking robust validation to ensure the highest specificity, sensitivity, and stability. Use Boster’s ELISA assay kits and you will have reproducible, publishable data lot after lot. As of 2019, our ELISA kits alone have been cited in 6,000+ publications globally.

Boster Bio is offering gene-editing services including but not limited to mutagenesis, tagging, and injection. Our service can significantly reduce your experiment design time and can be tailored to different experimental requirements.