Boster Biological Technology

Boster offers a three-phase custom mouse monoclonal antibody production service for a starting price of $4,000. We know that there are situations where you are unable to find the antibody you need for your research and Boster is here to help you with that! Our experienced monoclonal antibody production team will consult with you throughout the process to mitigate the risks of monoclonal antibody production. Our goal is not only to make an antibody that recognizes the antigen but to make an antibody that works for your specific application.

Turnaround time: 4-5 months

To get an order form, contact us at [email protected]

Below is our standard service broken down into the typical multi-phase development plan. We can easily customize various aspects of this to your specifications whenever necessary.

Phase 1 Immunization |Timeline: 6 weeks | Requirements: 3-5mg of antigen or immunogen sequence
We can use a variety of antigen types including but not limited to peptides and recombinant proteins. If you have questions about which one is better for your particular needs or want to use other types of antigen, please contact us. Once the antigen is determined, the first stage will begin with immunizing Balb/C mice and ELISA screening of polyclonal sera. You can add additional screening criteria into this step. The sera exhibiting highest titer will be used for fusion in the second stage.

Deliverables: Test bleed report. Optional: we can also provide 15ul of anti-sera for you to test.

Phase 2 Cell Fusion and Screening | Timeline: 7-8 weeks
Fuse the high titer splenocytes with mouse myeloma cells, grow cells in HAT medium and isolate the antigen positive hybridoma cells. In this step we will eliminate the failed fusions of splenocyte-to-splenocyte, myeloma-to-myeloma and unfused cells. After this first elimination there are usually 3000 successful clones left for an ELISA screening for antigen positive clones. This will further narrow the candidate pool to around 10 clones for subcloning. Before subcloning, we will send the supernatant of 2 of the ELISA positive clones to you for your evaluation.

Deliverables: Cell cuture testing report. 1ml supernatant of each selected clone.

Phase 3 Subcloning, Expansion, and Delivery | Timeline: 6-7 weeks
Once you select two clones out of the final candidate pool, we will isolate these clones of interests using the cloning by limiting dilution method. We will expand these clones of interests into monoclonal colonies which are then ready for cryopreservation. We will produce a small batch of monoclonal antibodies using 3 mice via ascites production and purify the antibodies via protein A.

Deliverables: Protein A purified monoclonal antibody 1-3mg per clone; 2 hybridoma clones; 6 months of cell banking service.

Pricing options:
$4,000 - User Provide Antigen
-The most economical solution on the market for custom monoclonal antibody using user provided antigen. Basic screening via direct ELISA.
$4500- Boster Provide Antigen
-Do not have antigen protein? No worries. We will synthesize a recombinant protein for you for only $500.
Custom -Fully Customizable
-Contact us for a free consultation. See details in Additional Services below. We will try our best to accommodate all of your custom requests.
Additional Services:
Recombinant Protein, Custom Screening Criteria, Immunizing More Mice, Additional Clones, Antibody Production, Antibody Purification, Antibody Conjugation, Cell Banking, and Hybridoma Sequencing.

Boster Fail Safe for Monoclonal Service
If a successful clone cannot be found, customer has the following options:
1. Cancel the project after the first delivery of supernatants and only be charged $1,000 for package 1 and $1,500 for package 2 (will still receive recombinant proteins).
2. Purchase an additional 10 supernatants for $999. If a successful clone cannot be found after the second supernatant delivery, customer may choose to cancel the project and only be charged $1,000/$1,500 plus the cost of the additional 10 supernatants, for a total of $1,999/$2,499