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Bon Opus Biosciences

4 Orders Completed
Millburn, New Jersey, US

About Bon Opus Biosciences

Bon Opus offers expert service like no one else. With decades of experience in every field, Bon Opus brings the best and brightest to each project. Our boutique size and structure allow for us to work closely with you to make the best experience possible. Ollie the Otter is always putting a smile on our faces,... Show more »

Bon Opus offers expert service like no one else. With decades of experience in every field, Bon Opus brings the best and brightest to each project. Our boutique size and structure allow for us to work closely with you to make the best experience possible. Ollie the Otter is always putting a smile on our faces, and all of us at Bon Opus strive to do the same for you. We genuinely care about your research, and know you deserve only the best. Bon Opus will be with you at every step of the way.

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Our Services (27)


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Custom Polyclonal Antibody Production

Price on request

Our polyclonal antibody experts can start straight from your provided peptide sequence or design peptide sequence, and finish your project to your exact specifications in less than 15 weeks. Our high standards include antigen affinity purification and ELISA identification (>1:32000) to help prove beyond a doubt our quality and... Show more »

Our polyclonal antibody experts can start straight from your provided peptide sequence or design peptide sequence, and finish your project to your exact specifications in less than 15 weeks. Our high standards include antigen affinity purification and ELISA identification (>1:32000) to help prove beyond a doubt our quality and dedication to providing you exactly what you need: your research deserves the best.

Standard Package
The Ollie Package involves the standard immunization, screening, antigen affinity purification and ELISA validation for your Antibody Production. The Ollie Package includes 5 milestones, and finishes within 12-15 weeks.

Milestones

  1. Peptide design and synthesis
  2. Peptide conjugation to carrier protein (KLH, BSA, DVA)
  3. Immunization of two rabbits
  4. ELISA identification (>1:32000)
  5. Antigen affinity purification

Final Deliverable
0.5-1ml of pre-immune Sera per rabbit
2-3mg antigen Peptide
2-5mg Purified Antibody per project
Certificate of Analysys
Data Package

Custom Options
If the Ollie Package doesn’t perfectly fit your requirements, feel free to create your own package! Please indicate your desired quantities of Pre-Immune Sera, Peptide, and Antibody. You can create your own package using the same sheet as before. Possible customization options include:

Antibody Identification
ELISA
Dot Blot
Western Blot
IHC
Antibody Labeling
HRP
Biotin
FITC

Data Packages Contain:
Identification data
HPLC Report
Mass Spec

Why Polyclonal?
When comparing polyclonal and monoclonal antibodies, there are benefits to each, depending on your application and needs. Polyclonal antibodies tend to have a higher affinity, which can increase the signal of a protein with a lower expression level. Polyclonal AB also recognize a number of binding sites for antibodies on your antigen. This allows for denatured protein to be detected more easily.

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Peptide Synthesis

Price on request

Bon Opus Bio provides high quality peptide synthesis service of up to 149 residues in length, with a scale ranging from milligrams to kilograms.

Modifications
Amidation, Quenched Fluorescence Peptide, Unusual Amino Acid, Acetylation, Fluorescence/Dye Modification, Cyclic Peptide, Phosphorylation, MAPS and Carrier Complex,... Show more »

Bon Opus Bio provides high quality peptide synthesis service of up to 149 residues in length, with a scale ranging from milligrams to kilograms.

Modifications
Amidation, Quenched Fluorescence Peptide, Unusual Amino Acid, Acetylation, Fluorescence/Dye Modification, Cyclic Peptide, Phosphorylation, MAPS and Carrier Complex, Isotope Labeling, N&C Terminal Modification

Peptide Synthesis

Quantity: From mg to kg
Purity: Up to >98%
Length: Up to 149aa
Data: HPLC and MS
Delivery: Less than 30aa Within 2 weeks
Price: From $2.60/aa

Peptide Library

Quantity: Up to 20mg
Purity: Up to >98%
Length: 5-20aa
Data: HPLC and MS
Delivery: 3 Weeks
Size: 48+ Peptides

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Custom Monoclonal Antibody (mAb) Production

Price on request

Bon Opus will get you the highest quality monoclonal antibody for your recombinant protein based antigen, delivering 4 clones, and >1mg Purified Antibody.

Antigen design: Provide your target protein sequence and our expert will review the sequence and design specific antigen

Antigen Production- Recombinant Protein is made... Show more »

Bon Opus will get you the highest quality monoclonal antibody for your recombinant protein based antigen, delivering 4 clones, and >1mg Purified Antibody.

Antigen design: Provide your target protein sequence and our expert will review the sequence and design specific antigen

Antigen Production- Recombinant Protein is made in house with choice of expression system:
E.coli
Yeast
Insect cells
Mammalian cells
Peptide: KLH-conjugated peptide

Immunization and Fusion:
Immunization of 4 mice
Boost Immunizations
Bleed and Monitor the immune response by ELISA
Fusion and subclone

Screening
1st supernatant screening by ELISA
2nd supernatant screening by ELISA and Western Blot
3rd supernatant screening by Flow Cytometry
4th supernatant screening by immunohistochemistry or immunocytochemistry
5th supernatant screening for mAb/mAb Sandwich ELISA

Antibody Production: Scale up positive clones purify antibody with Protein G beads

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Bacterial Protein Expression

Price on request
Request a quote for more information about this service.

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Gene Synthesis

Price on request

Bon Opus offers gene synthesis from any Gene Length, to help meet different project needs with the upmost reliability and efficiency. Our Gene Synthesis service is able to effectively complete enzymatic assembly on the basis of short DNA fragments, so we may generate your requested sequence up to 200kb in length. We significantly... Show more »

Bon Opus offers gene synthesis from any Gene Length, to help meet different project needs with the upmost reliability and efficiency. Our Gene Synthesis service is able to effectively complete enzymatic assembly on the basis of short DNA fragments, so we may generate your requested sequence up to 200kb in length. We significantly optimize the sequences using technology, and can provide synthesized genes with 100% accuracy. Bon Opus uses de novo synthesis, and Sanger sequencing to offer the highest quality output for our customers, designed to fit all your gene synthesis needs.

Base Pair Length: Price, Timeline
Up to 250: $125, 5-10 Days
250-1500: $0.25/bp, 5-10 Days
1501-3000: $0.27/bp, 12-15 Days
3001-4500: $0.30/bp, 15-20 Days
Over 4500: Please Inquire

Deliverables:
4ug Lyophilized Plasmid DNA
Sequencing Chromatogram
Certificate of Analysis

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Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
Price on request
Request a quote for more information about this service.

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Plasmid DNA Preparation

Price on request

For high quality plasmid DNA, Bon Opus's Plasmid DNA Preparation services are perfect for your needs. All plasmids are free of animal-derived materials and, upon request, can contain extremely low levels of endotoxins. Bon Opus DNA is perfect for transfection, antibody preparation, and other research. Full documentation and... Show more »

For high quality plasmid DNA, Bon Opus's Plasmid DNA Preparation services are perfect for your needs. All plasmids are free of animal-derived materials and, upon request, can contain extremely low levels of endotoxins. Bon Opus DNA is perfect for transfection, antibody preparation, and other research. Full documentation and project management ensures the highest quality product and experience possible.

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Protein Expression in Yeast

Price on request
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PCR

Price on request

Bon Opus PCR Cloning & Subcloning services allow for amplified DNA sequences. For cloning, Bon Opus offers sequence optimizations to verify the sequence to your exact needs. We will customize your project to your every request by designing primers, cloning PCR Products into a new vector, and verifying the result using Sanger sequencing.

Bon Opus PCR Cloning & Subcloning services allow for amplified DNA sequences. For cloning, Bon Opus offers sequence optimizations to verify the sequence to your exact needs. We will customize your project to your every request by designing primers, cloning PCR Products into a new vector, and verifying the result using Sanger sequencing.

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Baculovirus/Insect Cell Protein Expression

Price on request
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DNA Subcloning

Price on request

Bon Opus PCR Cloning & Subcloning services allow for amplified DNA sequences. For subcloning, Bon Opus utilizes the transformation of the template sequences between vectors with the same enzyme site restrictions, while offering high quality output, with the full economic and efficiency benefits that our subcloning entails.

Bon Opus PCR Cloning & Subcloning services allow for amplified DNA sequences. For subcloning, Bon Opus utilizes the transformation of the template sequences between vectors with the same enzyme site restrictions, while offering high quality output, with the full economic and efficiency benefits that our subcloning entails.

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Site-Directed Mutagenesis

Price on request

With our Site-Directed Mutagenesis service, Bon Opus can create specific DNA Mutations, such as point, insertion, or deletion mutations, and these mutations can occur at multiple sites. Site-Directed Mutagenesis are very useful in gene research due to the effects on character and characterization of the protein, and are widely... Show more »

With our Site-Directed Mutagenesis service, Bon Opus can create specific DNA Mutations, such as point, insertion, or deletion mutations, and these mutations can occur at multiple sites. Site-Directed Mutagenesis are very useful in gene research due to the effects on character and characterization of the protein, and are widely used in studies into protein functional site structure, functions & interactions of DNA components, activity optimization for enzymes, gene therapy, and more.

The following pricing is for Site-Directed Mutagenesis based on provided templates, without Gene Synthesis. If your project includes Gene Synthesis prior to SDM, please request a quote for heavily discounted pricing.

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De Novo Antibody Sequencing

Price on request

We have developed a protein de novo sequencing technology to determine the amino acid sequence of an unknown antibody with mass spectrometry. The technology is based on the mass spec measurement of the protein molecules, and does not require the access to the cell line. What we require is merely 0.2mg of the protein. Within three... Show more »

We have developed a protein de novo sequencing technology to determine the amino acid sequence of an unknown antibody with mass spectrometry. The technology is based on the mass spec measurement of the protein molecules, and does not require the access to the cell line. What we require is merely 0.2mg of the protein. Within three weeks, you will receive a detailed report from us, which includes the amino acid sequences of the heavy and light chains, the supporting data for the correctness of the sequence, as well as other observations we have made about the molecule.

Deliverable: A comprehensive report including the full sequences of the heavy and light chains, the supporting data for the sequence correctness, and other observations such as irregular modifications on the antibody.

Turnaround time: 15 business days after receiving the sample.

Service guarantee:

• A 30X average coverage*. That is, on average, each amino acid of the protein is covered by 30 or more distinct** overlapping peptides.
• At least 5X coverage on the CDR regions.
• Each amino acid of the variable regions is supported by strong MS/MS signals in at least 3 distinct peptides.

  • We normally get >50X coverage in our sequencing projects.

** Two distinct peptides must have different sequences, modifications, charge states, or measured in different LC-MS runs. When counting the coverage depth, it is important to count only the distinct peptides because repeated scans of the same peptide in the same LC-MS run do not provide much orthogonal information.

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Polyclonal Antibody Production

Price on request
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Hybridoma Sequencing

Price on request

A fast and cost-efficient alternative to our antibody sequencing service; our Antibody Hybridoma Sequencing is a fast and cost-efficient alternative. For as low as $800, you can have your sample characterized in just a few days. In addition, you will have a material ready for various molecular manipulations, including assembling... Show more »

A fast and cost-efficient alternative to our antibody sequencing service; our Antibody Hybridoma Sequencing is a fast and cost-efficient alternative. For as low as $800, you can have your sample characterized in just a few days. In addition, you will have a material ready for various molecular manipulations, including assembling desirable expression cassettes suitable for transient and stable expression, so you would never have to worry about your sample.

For a regular submission, all we need is approximately 10^5 cells that can be submitted as live/frozen cells, RNA-stabilized cells, or as lysed cells. If you do not have that many to spare, no worries! For a little bit extra, we can work with any sample size; down to a single cell. We work with all classes of immunoglobulins from all commercial species, and these projects are performed, and these antibodies are made, all in the U.S.A.!

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Biology

Price on request
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DNA Synthesis and Probe Development

Price on request
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Mutagenesis

Price on request
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DNA Services

Price on request
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Nucleic Acid Services

Price on request
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Antibody Services

Price on request
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Therapeutic Monoclonal Antibody (mAb) Development

Price on request
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Biochemistry & Molecular Biology

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Synthetic Chemistry

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Protein Production

Price on request
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Chemistry and Materials

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Protein Services

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Bon Opus Biosciences Services

Inside Sales Manager
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Novoprotein Scientific Services

Vice President at Novoprotein

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