Biozilla

Biozilla is proud to offer you the best plasmid DNA preparation service available. Let us purify your plasmid at the fastest turnaround time in the industry using our proprietary method. We guaranty excellent DNA quality and purity at unbeatable prices.

We have optimized conventional silica-based anion-exchanger techniques to maximize plasmid DNA recovery and purity. The yields shown below are typical yields for high copy number plasmids but the recovery is influenced by the size and sequence of the plasmid and may varies between plasmids.

1) Miniprep (Performed in tube format, not plate format) (5 - 10 ug DNA)
- 1 to 10 plasmids / $3.90 each / 2-3 days
- 11 to 20 plasmids / $3.30 each / 2-3 days
- 21 to 50 plasmids / $2.60 each / 2-3 days
- 51 to 100 plasmids / $2.30 each / 2-3 days
- >100 plasmids / $1.90 each / ≥3 days

2) Petiteprep (0.2 - 0.5 mg DNA)
- 1 to 7 plasmids / $25 each / 2-3 days
- 8 to 15 plasmids / $22 each / 2-3 days
- 16 to 25 plasmids / $19 each / 2-3 days
- 26 to 39 plasmids / $17 each / 3-4 days
- ≥ 40 plasmids / $15 each / ≥4 days

3) Regular Maxiprep (0.5 - 1.0 mg DNA)
- 1 to 7 plasmids / $39 each / 2-3 days
- 8 to 15 plasmids / $34 each / 2-3 days
- 16 to 25 plasmids / $29 each / 2-3 days
- 26 to 39 plasmids / $27 each / 3-4 days
- ≥ 40 plasmids / $25 each / ≥4 days

4) High Yield Maxiprep (1.0 - 1.5 mg DNA)
- 1 to 3 plasmids / $55 each / 2-3 days
- 4 to 7 plasmids / $49 each / 2-3 days
- 8 to 15 plasmids / $45 each / 2-3 days
- 16 to 25 plasmids / $39 each / 3-4 days
- 26 to 39 plasmids / $37 each / 3-4 days
- ≥ 40 plasmids / $35 each / ≥4 days

5) Gigaprep (8 - 12 mg DNA)
- 1 to 4 plasmids / $199 each / 3-5 days
- ≥ 5 plasmids / $179 each / ≥5 days

Replicates (each): $2 (Miniprep) / $15 (Petiteprep) / $25 (Regular Maxiprep) / $35 (High Yield Maxiprep) / $179 (Gigaprep)
Endo-free: +$5 (Petiteprep) +$10 (Regular Maxiprep) / +$15 (High Yield Maxiprep) / +$89 (Gigaprep)
Yield Booster*: +$13 (Petiteprep) / +$19 (Regular Maxiprep) / +$29 (High Yield Maxiprep) / +$169 (Gigaprep)
DNA sequencing: $9.90/reaction
*The Yield Booster treatment is design to improve the yield of low copy number or difficult to amplified plasmids. This approach double the amount of prepared culture and allows its smooth processing throughout the anion-exchanger.

Material provided by customer: >500 ng plasmid
Deliverable:
- DNA quantification
- Spectrophotometric ratios
- Agarose gel electrophoresis

Shipping:  Transit time varies based on location, typically 1-2 days for Asia and 2-3 days for America and Europr. Use our EASYSHIPPING as convenient and affordable shipping method.

We can rapidly express and purify various types of antigens (from mg to g amounts) and have them ready for your specific application.

We developed a series of medium- to high-throughput scalable screening platforms to rapidly identify and optimize a preparation process tailored for every protein. This approach enables us to produce soluble recombinant proteins of the highest purity and activity.

Protein expression and purification screen:
We typically start by cloning the gene of interest into various proprietary solubility-enhancer vectors (more than 50 to choose from), then express the proteins under multiple conditions and purify from the resulting soluble fraction. The end point of our screens is a SDS-PAGE of the eluted materials. Screens start at only $249 (6 conditions).
Depending on the nature of your recombinant protein(s), we have a targeted screen for it: E. coli cytoplasmic or periplasmic expression, E. coli chaperon and/or binding partner co-expression,...

Optimization of protein expression and purification:
Intermediate scale up if necessary.

Protein expression and purification scale up:
- Culture capacity: up to 18 L per day.
- Downstream purification processes tailored to your recombinant protein(s) needs.
- Fast turnaround time and best price guarantied.

DNA cloning in any vector as low as $179. Directional cloning with no need of restriction sites + possibilities to modify vector and/or insert flanking regions. Validation by DNA sequencing of the insert. Price varies based on insert size, vector type and number of ordered clonings.

At Biozilla, we understand that every protein is unique and needs to be treated accordingly. Protein diversity provides nature with an infinite combination of tools but it also creates major challenges in the preparation of recombinant proteins. To tackle these issues, we developed a series of medium- and high-throughput screening platforms to rapidly identify and optimize a preparation process tailored for your protein. This approach enables us to produce soluble recombinant proteins of the highest purity and activity.

Our small scale screening processes have been designed with the scale up in mind so that larger amounts of protein can be rapidly prepared without intermediate steps. This screening approaches are directly scalable, cutting down on timely and expensive rounds of optimization.

Our main screening platform is designed for E. coli expression but our approach is versatile and can be adapted to various expression systems. We can accommodate small screens (just few data points) or larger ones (plate format).

Most protein expression/purification screens are performed in just 4-5 days from plasmid transformation to the end point of the screen. This service starts at only $249 and the full list of prices can be found at https://www.biozilla.bio/protein-optimization-screens.

We offer several scale up options for large scale protein preparation in bacteria (E. coli) and yeast (P. pastoris). We have prepared multiple types of recombinant proteins in the past, yielding from milligram to gram amounts of protein using common purification approaches.

We recommend to first optimize the expression and purification conditions using our screening platform prior to scale up.

​The purified proteins are typically shipped in dry ice in the recommended storage buffer but we can also aliquot and lyophilize your final product.

Turnaround times are based on the amount of initial material (culture volume) but most protein expression/purification scale ups are performed in just 4-5 days.

The full list of prices of protein expression and purification services can be found at https://www.biozilla.bio/protein-scale-up. For us to generate a quote, please give us the following info:
- Name and sequence of the protein(s) to screen.
- If you submit a plasmid, name of the vector, antibiotic resistance and type of induction.
- Number of conditions/variables to screen. Let us know if you need specific conditions to test and if you expect the protein(s) to be relatively difficult or easy to express.

2nd generation Site-Directed Mutagenesis service which offers the following advantages over traditional approaches:
- Efficient: Substitutions or insertion of up to 15 consecutive bp; fragment deletion of unlimited size. Co-modifications at two or three different sites. All these in a single reaction using a polymerase with 10 times higher fidelity than Pfu (used in Quickchange kits). Construction of mutant libraries. Amazing new possibilities!
- Affordable: $79 to $99 per reaction (based on number of requested units) which makes this service cheaper than using a kit!
- Rapid: Mutated plasmid(s) (Miniprep) typically prepared within just few days after reception of the original plasmid.

• Price:
  $99 per mutation for 1 to 5 units / $94 per mutation for 6 to 10 units / $89 per mutation for 11 to 15 units / $84 per mutation for 16 to 20 units / $79 per mutation for 21 to 30 units / Request a quote for >30 units.
  Extra $19 and $39 per unit for respectively 6-8 kb and 8-10 kb.

• Deliverable:
  Miniprep and DNA sequencing surrounding the mutated sequence.

Ready to start?
Please provide us the following information and we will send you a corresponding quote:
1) Plasmid size.
2) Insert nucleotide sequence (text files preferred).
3) Desired mutation(s) (e.g. R657D or G34 to D66 deletion).

Fully optimized yeast system available from Biozilla for your protein (cytoplasmic and secreted proteins).

We offer several scale up options for large scale protein preparation in bacteria (E. coli) and yeast (P. pastoris). We have prepared multiple types of recombinant proteins in the past, yielding from milligram to gram amounts of protein using common purification approaches.

We recommend to first optimize the expression and purification conditions using our screening platform prior to scale up.

​The purified proteins are typically shipped in dry ice in the recommended storage buffer but we can also aliquot and lyophilize your final product.

Turnaround times are based on the amount of initial material (culture volume) but most protein expression/purification scale ups are performed in just 4-5 days.

The full list of prices of protein expression and purification services can be found at https://www.biozilla.bio/protein-scale-up. For us to generate a quote, please give us the following info:
- Name and sequence of the protein(s) to screen.
- If you submit a plasmid, name of the vector, antibiotic resistance and type of induction.
- Number of conditions/variables to screen. Let us know if you need specific conditions to test and if you expect the protein(s) to be relatively difficult or easy to express.

2nd generation Site-Directed Mutagenesis service which offers the following advantages over traditional approaches:
- Efficient: Substitutions or insertion of up to 15 consecutive bp; fragment deletion of unlimited size. Co-modifications at two or three different sites. All these in a single reaction using a polymerase with 10 times higher fidelity than Pfu (used in Quickchange kits). Construction of mutant libraries. Amazing new possibilities!
- Inexpensive service: $79 to $99 per reaction (based on number of requested units) which makes this service cheaper than using a kit!
- Rapid: Mutated plasmid(s) (Miniprep) typically prepared within just few days after reception of the original plasmid.

• Price:
  $99 per mutation for 1 to 5 units / $94 per mutation for 6 to 10 units / $89 per mutation for 11 to 15 units / $84 per mutation for 16 to 20 units / $79 per mutation for 21 to 30 units / Request a quote for >30 units.
  Extra $19 and $39 per unit for respectively 6-8 kb and 8-10 kb.

• Deliverable:
  Miniprep and DNA sequencing surrounding the mutated sequence.

Ready to start?
Please provide us the following information and we will send you a corresponding quote:
1) Plasmid size.
2) Insert nucleotide sequence (text files preferred).
3) Desired mutation(s) (e.g. R657D or G34 to D66 deletion).

We offer several scale up options for large scale protein preparation in bacteria (E. coli) and yeast (P. pastoris). We have prepared multiple types of recombinant proteins in the past, yielding from milligram to gram amounts of protein using common purification approaches.

We recommend to first optimize the expression and purification conditions using our screening platform prior to scale up.

​The purified proteins are typically shipped in dry ice in the recommended storage buffer but we can also aliquot and lyophilize your final product.

Turnaround times are based on the amount of initial material (culture volume) but most protein expression/purification scale ups are performed in just 4-5 days.

The full list of prices of protein expression and purification services can be found at https://www.biozilla.bio/protein-scale-up. For us to generate a quote, please give us the following info:
- Name and sequence of the protein(s) to screen.
- If you submit a plasmid, name of the vector, antibiotic resistance and type of induction.
- Number of conditions/variables to screen. Let us know if you need specific conditions to test and if you expect the protein(s) to be relatively difficult or easy to express.