After more than a decade of experience in the sale and marketing of research products for “Life Science Research”, Werner Hassler, Switzerland and Gunther Jaeger, Germany founded the Cologne based BIOTREND Chemikalien GmbH in May 1988.
BIOTREND was instigated in order to meet the growing demand from customers for an independent and technologically orientated distribution facility offering high quality, yet cost-effective products for biochemical, immunochemical and pharmaceutical research.
Through our innovative strength we are not only providing our customers a vast product range, but also a high quality, first-class service package.
At present we offer more than 90.000 products that have successfully been implemented not only in the drug discovery market, but also in cancer research as well as other scientific fields.
BIOTREND's custom production of polyclonal antibodies offers you the following services:
Immunisation of 2 rabbits
Delivery of 2 x 1,5 ml preimmune serum
Cost of the animals plus housing and care for approx. 7 weeks
Serum collection (20 ml per animal for testing plus the serum from final bleeding)
Price: EUR 535,-
We also offer additional complete immunisation packages which include peptide synthesis and conjugation or affinity purification against the antigen
5 mg of FITC labelled conjugate
5 mg of CFTM Dye labelled conjugate
Price includes Certificate of Analysis and selective precipitation of starting material if required.
2-4 mg of HRP conjugated Antibody
Price includes Certificate of Analysis and Ig-Fraction production prior to conjugation with removal of free HRP.
2-4 mg of AP conjugated Antibody
Price includes Certificate of Analysis and Ig-Fraction production prior to conjugation.
This conjugation is designed to yield negligible free enzyme. Therefore a removal step is normally not required.
Custom Labelling with Gold Particles
We can label almost any protein, antibody an most peptides with gold particles of sizes ranging from 0,8 nm to 25 nm.
Please contact us for details or visit AURION.
5 mg of biotinylated Antibody
Price includes Certificate of Analysis and immunoglobulin fraction production prior to conjugation.
Other conjugations include:
[125I] Iodination Service
For the iodination approx. 0,5 - 1,0mg of the cold compound is required.
[3H] Tritiation Service
Catalytic reduction with Tritium gas (Pd/C)
The Tritium labelling Service includes the reaction of the precursor according to the method of choice as well as removing catalyst and labile tritium.
We also offer HPLC purification and characterisation of the final products according to GLP.
We encourage you to discuss your specific requirements with us.
Tritiation Handling System- [3H2]
Complete 3H-Technology (incl. dry HV-pumps)
Custom monoclonal antibodies without limitation of positive clones
Price: Euro 3990,-
Duration of project: 4 to 6 months after receipt of an antigen (peptide/protein).
Quantity: 3 cryo cultures and 10 ml supernatant from each hybridoma cell line
Development of hybridomas includes the following steps/components:
1.Planning, preparation work, conjugation, if necessary
2.Immunisation, cell fusion, selection and screening
This part contains: Immunisation of at least 10 mice, cultivation of the necessary myeloma line, all steps for cell fusion, including selection on HAT medium and screening. The customer receives detailed protocols and information concerning the schedule and the tests after conclusion of this part.
This part contains: Two clonings including screening and cultivation of positive cultures after the second cloning. The custo-mer receives 1 ml solution of supernatant for each positive clone for testing in his own system.
4.Final cloning and Cryo cultures
This part contains final clonings and all preparatory steps to obtain pure clones. Three cryo cultures will be pre-pared for each positive stable clone. The cryo cultures and 10 ml of cell culture supernatant of each clone will be delivered to the customer. Furthermore, two cryo cultures can be prepared upon request of the client and be retained for security reasons for at least five years. These cultures will neither be used by BIOTREND nor a third party.
The customer receives complete documentation of all working steps.
After each phase of successful completion.
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