1352598199381 67ccca5ba84224cd4ccc49bd3594cb15

Biotechnology Research and Education Program

College Park, Maryland, US

The Biotech Research and Education Program (BREP) at the University of Maryland is the regions premier biotechnology, biopharmaceutical and biofuel research center. The program consists of two core facilities dedicated to providing supplemental research to academia and industry.

Founded in 1985, The Bioprocess Scale-Up Facility (BSF) is the Biotechnology Research and Education Program's modern bioprocessing laboratory dedicated to the development and scale-up of fermentation biotechnology products and processes, specifically bacteria and yeast. With over 1500 fermentations conducted since 1998, the BSF has accelerated the R&D of local biotechnology leaders such as Human Genome Sciences, NIH and MedImmune, as well as growing Maryland start-ups such as Martek Biosciences and Digene Corporation.

The BSF offers a broad range of bioprocess scale-up and production services. The facility is ideal for... Show more »

The Biotech Research and Education Program (BREP) at the University of Maryland is the regions premier biotechnology, biopharmaceutical and biofuel research center. The program consists of two core facilities dedicated to providing supplemental research to academia and industry.

Founded in 1985, The Bioprocess Scale-Up Facility (BSF) is the Biotechnology Research and Education Program's modern bioprocessing laboratory dedicated to the development and scale-up of fermentation biotechnology products and processes, specifically bacteria and yeast. With over 1500 fermentations conducted since 1998, the BSF has accelerated the R&D of local biotechnology leaders such as Human Genome Sciences, NIH and MedImmune, as well as growing Maryland start-ups such as Martek Biosciences and Digene Corporation.

The BSF offers a broad range of bioprocess scale-up and production services. The facility is ideal for those seeking innovative research in bacterial and yeast fermentations in the biopharmaceutical and biofuel industries. With production volumes of up to 250L, the BSF is designed to bolster the areas burgeoning biotech industry. The facility is also capable of performing advanced separations, protein purification and product analysis.

Our most recent facility, the Biopharmaceutical Advancement Facility (BAF) specializes in the development of cell culture-based biopharmaceutical products. The facility houses equipment that can culture adherent or suspension-adapted cell lines. Specific services offered include: antibody and protein production, cell bank production and short term storage, bioreactor process development, scale-up and scale-down studies, and cell clarification. Facility staff offers extensive experience with a range of cell lines: HEK293, CHO, Sf9, NSO, MSCs, and many other primary cell lines.

Both facilities, located in Maryland, are not restricted by current good manufacturing practices (cGMP) requirements, however production processes are directly transferable to our partners operating under cGMPs (such as Paragon Bioservices in Baltimore, MD and the Walter Reed Army Institute of Research).

Certifications: GLP, BSL-2, BSL-1
Cell lines: full access to all BSL-2, BSL-1 cell lines from ATCC

Instrumentation:
- New Brunswick Bioflo310 Cell Culture Bioreactors (1.0, 3.5, 5.0, 10.0 working volume)
- New Brunswick Scientific Fermentation bioreactors
• Bioflo 6000 – 200L
• Bioflo 310 – 2.5L and 10L
• Bioflo Pro – 100L
• Bioflo 5000 – 60L
- Beckman Coulter Counter
- Nexcelom Cellometer Auto T4
- Nova Biomedical BioProfile Flex Analyzer
- Sartorius Biowelder
- Thermo CO2 Incubators
- New Brunswick Innova44R Shaker/Incubator
- Centrifuges
• Sharples – large bowl and small bowl continuous,
• CEPA – large bowl continuous,
• Beckman Coulter Avanti J-20XP,
• Thermo Electron Corporation IEC Micromax RF microcentrifguge
- Chromatography (Bio-Rad NGC, 2 x Bio-Rad BioLogic LP)
- Ultrafiltration/Diafiltration (Millipore Pellicon-2, Millipore 1)
- Microfluidics Microfluidizer Processor M-110EH
- YSI 2700 glucose analyzer
- BioTek Synergy HT plate reader
- Bio-Rad Mini Protean electrophoresis setups
- Wyatt Multi-Angle Light Scattering (MALS), connected to a Waters Alliance HPLC system for SEC MALS.
- Jasco FP-8200 Fluorescence Spectrophotometer with a Peltier temperature cell.
- Jasco 710 Spectropolarimeter (Circular Dichroism)
- Horiba LB-550 Dynamic Light Scattering (DLS).

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Biotechnology Research and Education Program has not listed any services.

Bacterial cell culture
Starting at $1,100.00 per run

Bacteria and yeast fermentation services

per fermentation – up to 48 hours - total volume listed.
10L - $1100 – New Brunswick Eppendorf Bioflo310
60L - $2700 - New Brunswick Eppendorf Bioflo5000
100L - $3400 - New Brunswick Eppendorf Bioflo Pro
200L - $5000 - New Brunswick Eppendorf – Bioflo 6000

Turnaround time: ~2... Show more »

Bacteria and yeast fermentation services

per fermentation – up to 48 hours - total volume listed.
10L - $1100 – New Brunswick Eppendorf Bioflo310
60L - $2700 - New Brunswick Eppendorf Bioflo5000
100L - $3400 - New Brunswick Eppendorf Bioflo Pro
200L - $5000 - New Brunswick Eppendorf – Bioflo 6000

Turnaround time: ~2 weeks for fermentation; 4-6 weeks for cell culture

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Biochemical engineering
Price on request

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more... Show more »

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Bioprocess Development and Scale-Up
Price on request

The cornerstone facility in the Mid-Atlantic with 30 years of contract research scale-up projects.

Any cell line (BSL1 or BSL2) can be scaled to 250L (bacteria and yeast) and 4 x 10L suspension cell culture. Prices are available in other services listed.

The cornerstone facility in the Mid-Atlantic with 30 years of contract research scale-up projects.

Any cell line (BSL1 or BSL2) can be scaled to 250L (bacteria and yeast) and 4 x 10L suspension cell culture. Prices are available in other services listed.

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Cell fermentation
Starting at $1,100.00 per run

Bacteria and yeast fermentation services

per fermentation – up to 48 hours - total volume listed.
10L - $1100 – New Brunswick Eppendorf Bioflo310
60L - $2700 - New Brunswick Eppendorf Bioflo5000
100L - $3400 - New Brunswick Eppendorf Bioflo Pro
200L - $5000 - New Brunswick Eppendorf – Bioflo 6000

Turnaround time: ~2... Show more »

Bacteria and yeast fermentation services

per fermentation – up to 48 hours - total volume listed.
10L - $1100 – New Brunswick Eppendorf Bioflo310
60L - $2700 - New Brunswick Eppendorf Bioflo5000
100L - $3400 - New Brunswick Eppendorf Bioflo Pro
200L - $5000 - New Brunswick Eppendorf – Bioflo 6000

Turnaround time: ~2 weeks

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Cell ultra-filtration
Starting at $250.00 per run

UF/DF for protein separations and concentration.

$250 set-up plus
$5 per liter

filter not included

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media... Show more »

UF/DF for protein separations and concentration.

$250 set-up plus
$5 per liter

filter not included

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Chromatography Services
Starting at $80.00 per hour

Protein purification services

Equipment
Bio-Rad Biologic LP
Bio-Rad NGC
Fraction collection

$450 set up
$80 per hour with reproducible protocol
$110+per hour with no existing protocol

resin not included

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen,... Show more »

Protein purification services

Equipment
Bio-Rad Biologic LP
Bio-Rad NGC
Fraction collection

$450 set up
$80 per hour with reproducible protocol
$110+per hour with no existing protocol

resin not included

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Fluid Dynamics
Price on request

Turbulence and multiphase fluid dynamics

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification... Show more »

Turbulence and multiphase fluid dynamics

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Biospecimen Freeze Drying
Starting at $150.00 per sample

Tray freeze drying -
any combination of tubes or vials, up to 2 square feet of space.
This cost does not include labor for aliquoting.

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if... Show more »

Tray freeze drying -
any combination of tubes or vials, up to 2 square feet of space.
This cost does not include labor for aliquoting.

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Lyophilization
Starting at $250.00 per sample

Aseptic Vialing of BDS, API, or other liquid solutions (nonGMP), lyophilization via tray drying, (Labconco).
with stoppering

less than 100 vials - $250
each additional vial - $1 each - up to 500 total

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized... Show more »

Aseptic Vialing of BDS, API, or other liquid solutions (nonGMP), lyophilization via tray drying, (Labconco).
with stoppering

less than 100 vials - $250
each additional vial - $1 each - up to 500 total

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Mammalian cell culture
Starting at $500.00 per run

Mammalian, Insect, Plant, Stem, Bachilovirus cell culture

Process Equipment
Static and shaking incubators
T-Flask
Roller Bottle
Spinners
New Brunswick Scientific (NBS) Celligen 310 Bioreactors (working volume of 1.5, 2.5, 5.0,
and 10.0 liters)

$500 set up charge
$100 per day maintenance charge
$500 per... Show more »

Mammalian, Insect, Plant, Stem, Bachilovirus cell culture

Process Equipment
Static and shaking incubators
T-Flask
Roller Bottle
Spinners
New Brunswick Scientific (NBS) Celligen 310 Bioreactors (working volume of 1.5, 2.5, 5.0,
and 10.0 liters)

$500 set up charge
$100 per day maintenance charge
$500 per passage

media cost not incuded

Turnaround time: ~4-6 weeks

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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Metabolic engineering
Price on request

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more... Show more »

Turnaround time: ~1 week

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

« Show less
Protein Purification
Starting at $80.00 per hour

Protein purification services

Equipment
Bio-Rad Biologic LP
Bio-Rad NGC
Fraction collection

$450 set up
$80 per hour with reproducible protocol
$110+per hour with no existing protocol

Turnaround time: ~4-6 weeks

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell... Show more »

Protein purification services

Equipment
Bio-Rad Biologic LP
Bio-Rad NGC
Fraction collection

$450 set up
$80 per hour with reproducible protocol
$110+per hour with no existing protocol

Turnaround time: ~4-6 weeks

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

« Show less
Yeast culture
Starting at $1,100.00 per run

Yeast fermentation services

per fermentation – up to 48 hours - total volume listed.
10L - $1100 – New Brunswick Eppendorf Bioflo310
60L - $2700 - New Brunswick Eppendorf Bioflo5000
100L - $3400 - New Brunswick Eppendorf Bioflo Pro
200L - $5000 - New Brunswick Eppendorf – Bioflo 6000

Turnaround time: ~2 weeks for... Show more »

Yeast fermentation services

per fermentation – up to 48 hours - total volume listed.
10L - $1100 – New Brunswick Eppendorf Bioflo310
60L - $2700 - New Brunswick Eppendorf Bioflo5000
100L - $3400 - New Brunswick Eppendorf Bioflo Pro
200L - $5000 - New Brunswick Eppendorf – Bioflo 6000

Turnaround time: ~2 weeks for fermentation; 4-6 weeks for cell culture

Standard requirements:
Stable cell lines, frozen, vapor phase liquid nitrogen, Lyophilized cell line, Culture plate, glycerol stock.
Process SOPs (if available), growth conditions, media recipes or names, growth curves, sample timing etc. Purification processes, comparative literature, etc. The more information provided, the more accurate the estimate and less costly the project.

Deliverables:
- serum / tissue for animal studies, and powerpoint / pdf for final reports
- excel files for growth curves, data acquisition on some equipment.
- Fermentation logs
- Purified product
- Frozen stocks, frozen cell paste, cell lysates, filtered supernatant, dried cells/reagent

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June 3, 2014

Positive review received for Mammalian cell culture:

"Attentive, resourceful, exact. An excellent project experience."

December 12, 2012

Positive review received for Mammalian cell culture:

"Ben Woodard worked with our group to find the best culture material for our project, and was able to explain the pros and cons of different culture materials clearly to a non-specialist. His communications were always prompt, and the project was completed on time. I would definitely work with him again."

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