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BioPhysical-Solutions

Ambler, Pennsylvania, US

BioPhysical-Solutions was set up as a Discovery Partner to encourage wider utilization of Biophysical approaches in the Life Sciences. Biophysical techniques often exploit label-free observations to detect molecular interactions (binding) and/or conformational changes. Many of the tools are highly specialized, and the data generated may lead to key insights into molecular interactions. However, interpreting the results to yield definitive conclusions often benefits from the insight of technology experts. Thus the initiation of BioPhysical-Solutions.

BioPhysical-Solutions offers several unique label-free capabilities: Thermal Stability Analysis for protein formulation or compound binding, and the Corning EPIC technology for both biophysical binding and cell-based assays (see below). We also offer unique permutations of labeled technologies, such as Fluorescence Lifetime analysis (in high throughput, on a nano-sec... Show more »

BioPhysical-Solutions was set up as a Discovery Partner to encourage wider utilization of Biophysical approaches in the Life Sciences. Biophysical techniques often exploit label-free observations to detect molecular interactions (binding) and/or conformational changes. Many of the tools are highly specialized, and the data generated may lead to key insights into molecular interactions. However, interpreting the results to yield definitive conclusions often benefits from the insight of technology experts. Thus the initiation of BioPhysical-Solutions.

BioPhysical-Solutions offers several unique label-free capabilities: Thermal Stability Analysis for protein formulation or compound binding, and the Corning EPIC technology for both biophysical binding and cell-based assays (see below). We also offer unique permutations of labeled technologies, such as Fluorescence Lifetime analysis (in high throughput, on a nano-sec time scale) using pre-marketed instrumentation from Fluorescence Innovations, Inc.

Fluorescence Lifetime explores fluorescence decay as a function of time and is able to reduce small molecule screening artifacts. For example, using Fluorescence Lifetime to collect thermal stability data has enabled an over 20-fold reduction in the amount of protein required (compared to published procedures). When used to measure FRET assays, Fluorescence Lifetime has demonstrated > 10 fold reduction in noise, enabling a small FRET signal to yield statistically significant results. In one implementation, protein conformational changes were able to be pursued as therapeutic hypotheses.

BioPhysical-Solutions is located at the PABiotech in Doylestown, PA, and has access to one of the largest and best documented libraries of natural product extracts available for HTS. This library was developed by the Merck + Schering Plough companies, and was donated to the Blumberg foundation for Hepatitis B Research, forming the Natural Products Discovery Institute (NPDI). BioPhysical-Solutions offers access to this collection using it's label-free screening technologies.

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BioPhysical-Solutions has not listed any services.

High Throughput Screening (HTS)
Price on request

Many partners offer High Throughput Screening. Biophysical-Solutions emphasizes label-free technologies, and offers unparalleled capabilities using Thermal Shift Assay (TSA, DSF, or ThermoFluor) for biophysical binding, and the Corning EPIC technology for either biophysical binding, or cell-based assays.

Projects will be... Show more »

Many partners offer High Throughput Screening. Biophysical-Solutions emphasizes label-free technologies, and offers unparalleled capabilities using Thermal Shift Assay (TSA, DSF, or ThermoFluor) for biophysical binding, and the Corning EPIC technology for either biophysical binding, or cell-based assays.

Projects will be unique. Project scope and price will be developed with the discovery partner directly.

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Label-Free Ligand Binding Assays
Price on request

Corning® EPIC® Label-Free Screening Assays

Corning's label-free technology, the Epic, is a plate-based biosensor that measures mass changes near a well surface.
It can be used for biochemical screening, measuring fragment, small molecule, or antigen interacting with a binding partner adhered to the surface. In this... Show more »

Corning® EPIC® Label-Free Screening Assays

Corning's label-free technology, the Epic, is a plate-based biosensor that measures mass changes near a well surface.
It can be used for biochemical screening, measuring fragment, small molecule, or antigen interacting with a binding partner adhered to the surface. In this sense it is the only high-throughput biosensor capability currently available.
EPIC is also widely used to measure cellular effects, where it reports on therapeutics interacting with GPCRs, ion channels, receptor tyrosine kinases, cytokine interactions, and even viral entry. On stimulation, cells undergo morphological changes that are observed in this label-free assay. Such changes may include a change in cell shape, cytoskeletal rearrangements, or just migration of molecules to or from the membrane. This makes EPIC the choice for initial screening to find potential therapeutics that interact with your cellular system !
BioPhysical-Solutions was an early adopter of this technology and has several instruments able to contribute to your discovery programs.

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Corning® Epic®
Differential Scanning Fluorimetry (DSF)
Differential Scanning Fluorimetry
Price on request

DSF, also known as ThermoFluor, Thermal Shift Assay (TSA), Protein Thermal Shift Assay (PTSA), Protein Thermal Shift (PTS), and several other acronyms, has been widely used for fragment and small molecule hit finding, HTS, mode-of-action, and driving SAR. It has also been used for protein formulation purposes. ... Show more »

DSF, also known as ThermoFluor, Thermal Shift Assay (TSA), Protein Thermal Shift Assay (PTSA), Protein Thermal Shift (PTS), and several other acronyms, has been widely used for fragment and small molecule hit finding, HTS, mode-of-action, and driving SAR. It has also been used for protein formulation purposes.
BioPhysical-Solutions has a unique method of collecting thermal shift data, that utilizes a pre-marketed technology currently not available elsewhere, meaning it consumes at least an order-of-magnitude less protein per well than other hardware, making it the choice for HTS where protein resources are limited.
Projects are unique and will be designed based on client need, utilizing our ~20-year experience with this technology.

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Thermal Shift Assay (TSA)
Price on request

TSA, also known as ThermoFluor, Differential Scanning Fluorimetry (DSF), Protein Thermal Shift Assay (PTSA), Protein Thermal Shift (PTS), and several other acronyms, has been widely used for fragment and small molecule hit finding, HTS, mode-of-action, and driving SAR. It has also been used for protein formulation purposes. ... Show more »

TSA, also known as ThermoFluor, Differential Scanning Fluorimetry (DSF), Protein Thermal Shift Assay (PTSA), Protein Thermal Shift (PTS), and several other acronyms, has been widely used for fragment and small molecule hit finding, HTS, mode-of-action, and driving SAR. It has also been used for protein formulation purposes.
BioPhysical-Solutions has a unique method of collecting thermal shift data, that utilizes a pre-marketed technology currently not available elsewhere, meaning it consumes at least an order-of-magnitude less protein per well than other hardware, making it the choice for HTS where protein resources are limited.
Projects are unique and will be designed based on client need, utilizing our ~20-year experience with this technology.

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Protein Biophysical Characterization
Price on request
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Natural Product Screening
Price on request

With ~40% of all drugs derived from Natural Sources and half of these being unmodified Natural Products) continuing to probe natural libraries can be a rich source of novel lead molecules.

BioPhysical-Solutions has teamed with the Natural Products Discovery Institute (NPDI, www.npdi-us.org) to offer Label-Free HTS of the... Show more »

With ~40% of all drugs derived from Natural Sources and half of these being unmodified Natural Products) continuing to probe natural libraries can be a rich source of novel lead molecules.

BioPhysical-Solutions has teamed with the Natural Products Discovery Institute (NPDI, www.npdi-us.org) to offer Label-Free HTS of the NPDI Libraries. Natural libraries are NOT like other small molecule screening libraries...and their prosecution using common screening technologies will yield a rich hit set, largely consisting of artifacts. BioPhysical-Solutions has been prosecuting natural extract libraries for years, and has developed a streamlined process emphasizing label-free technologies, early selectivity profiling, and rapid deconvolution to identify novel naturals.

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Solubility and Dissolution Testing
Price on request

Corning's label-free technology, the Epic, has been widely used to measure compound aggregation. BioPhysical-Solutions can evaluate the behavior of your hit/lead molecules in this assay.

Corning's label-free technology, the Epic, has been widely used to measure compound aggregation. BioPhysical-Solutions can evaluate the behavior of your hit/lead molecules in this assay.

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Corning® Epic®
Biology
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Biochemistry & Molecular Biology
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Protein Services
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Protein Characterization
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Biomolecular Interaction Analysis
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Chemistry and Materials
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Analytical Chemistry Services
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Drug Discovery & Development
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Drug Discovery
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Hit Identification
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Natural Products
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2018-06-07 11:43:47 +0200

Net Promoter Score of 8 received for Thermal Shift Assay (TSA).

Additional Ratings: satisfaction with deliverable: 4, satisfaction with timeliness: 2.

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