1363460938966 fe628c600313d31f882803019bf44008

Biophysical Analysis Lab

West Lafayette, Indiana, US

The purpose of the Biophysical Analysis Lab (BAL) at the Bindley Biosciences Center is to provide complete biophysical analysis of macromolecules.

In the lab, there are two analytical ultracentrifuges: Beckman-Coulter XLI and XLA, a Biacore 3000, an ITC, and a SEC-MALS platform. With these instruments we can determine kinetic parameters, stoichiometry of binding, size distribution, shape, oligomerization state and other biophysical measurements. Complementing these traditional biophysical methods, the BAL also provides mass spectrometric analyses at both the protein and peptide levels. The lab strives to establish long-term collaborations and deliver professional quality data that can be used for publications and grant proposals.

Biophysical Analysis Lab has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
Analytical Ultracentrifugation (AUC)
Price on request

There are two analytical ultracentrifuges: a Beckman Coulter XLI and a Beckman Coulter XLA. The XLI is equipped with both Rayleigh Interference and absorbance optics. This provides a complete analysis both at low concentrations (~0.1 mg/ml) and samples without chromophores. The XLA is equipped with only absorbance optics and is... Show more »

There are two analytical ultracentrifuges: a Beckman Coulter XLI and a Beckman Coulter XLA. The XLI is equipped with both Rayleigh Interference and absorbance optics. This provides a complete analysis both at low concentrations (~0.1 mg/ml) and samples without chromophores. The XLA is equipped with only absorbance optics and is mainly used for protein/peptide analyses.

The information you can get from the analytical ultracentrifugation is dependent on the questions being asked. Routinely, sedimentation velocity experiments are employed. This type of experiment can be used to evaluate the stoichiometry and kinetics of interactions along with hydrodynamic and thermodynamic properties of the reacting systems. This technique can provide information on the sedimentation coefficient distributions, macromolecular shape distributions, equilibrium/rate constants and molecular weight distributions. Sedimentation equilibrium experiments can also be performed to determine kinetic constants for self associating systems. These experiments are conducted in the native buffer and do not rely on either immobilization or labeling of the samples.

« Show less
Fluorescence spectrophotometry
Price on request

The Cary Eclipse fluorimeter can be used for a variety of fluorescent based assays. This instrument is also equipped with a manual polarizer for polarization assays and a 96-well plate adapter for larger sample analysis.

The Cary Eclipse fluorimeter can be used for a variety of fluorescent based assays. This instrument is also equipped with a manual polarizer for polarization assays and a 96-well plate adapter for larger sample analysis.

« Show less
Isothermal Titration Calorimetry (ITC)
Price on request

ITC is the only instrument capable of measuring the delta H (enthalpy) of interactions. The stoichiometry along with the strength of the interaction (Kd) can be ascertained from ITC experiments. This instrument is the gold standard for pharma companies to fine tune the drug interactions. The ITC is ideal for small molecule-protein... Show more »

ITC is the only instrument capable of measuring the delta H (enthalpy) of interactions. The stoichiometry along with the strength of the interaction (Kd) can be ascertained from ITC experiments. This instrument is the gold standard for pharma companies to fine tune the drug interactions. The ITC is ideal for small molecule-protein based interactions but can be applied to any system e.g. Protein-DNA, RNA interactions. This system also does not require any specific labeling, immobilization or special buffers.

« Show less
Surface Plasmon Resonance (SPR)
Price on request

The Biacore 3000 is built on the platform that uses the Surface Plasmon Resonance (SPR) phenomena, which can be used to monitor the refractive index of a given medium near the surface of a chip. The Biacore 3000 uses a label free system to determine the kinetic parameters (kon/koff) of biomolecular interactions. Evaluation of the... Show more »

The Biacore 3000 is built on the platform that uses the Surface Plasmon Resonance (SPR) phenomena, which can be used to monitor the refractive index of a given medium near the surface of a chip. The Biacore 3000 uses a label free system to determine the kinetic parameters (kon/koff) of biomolecular interactions. Evaluation of the kinetic parameters (kon/koff) of a protein-ligand system will require the immobilization of either protein or ligand to the surface of the chip. The binding of analytes flowing of over the surface of the chip can be uses to describe kon (association) and koff (disassociation). The chips are re-useable and have 4 binding surfaces which can be evaluated simultaneously. The Biacore Evaluation software can be used to derive the kinetic parameters for several models.

« Show less
SEC-MALS
Size exclusion chromatography with multiangle light scattering detectors
Price on request

SEC- MALS experiments can provide information on the absolute molecular weight of a system in solution without the need for standards, labeling or immobilization. The SEC-MALS also provides information about the hydrodynamic radius of the system and can be applied to a variety of different samples: proteins, DNA, RNA,... Show more »

SEC- MALS experiments can provide information on the absolute molecular weight of a system in solution without the need for standards, labeling or immobilization. The SEC-MALS also provides information about the hydrodynamic radius of the system and can be applied to a variety of different samples: proteins, DNA, RNA, polysaccarides, virus, polymers, nanoparticles etc. This technique coupled with AUC can provide a complete biophysical characterization of your particle’s size and distribution.

« Show less
Biolayer Interferometry
Price on request

High throughput binding analysis instrument (Affinity Range 10 pM-0.1 mM)
Application: Kinetic analysis, small molecules library screening, fast protein quantitation, and assay development

Sample volume: 200 uL for 96 wells plate, 40 uL for 384 titled wells plate

High throughput binding analysis instrument (Affinity Range 10 pM-0.1 mM)
Application: Kinetic analysis, small molecules library screening, fast protein quantitation, and assay development

Sample volume: 200 uL for 96 wells plate, 40 uL for 384 titled wells plate

« Show less
Spectroscopy
Price on request
Request a quote for more information about this service.
Chromatography
Price on request
Request a quote for more information about this service.
Calorimetry
Price on request
Request a quote for more information about this service.
Spectrophotometry
Price on request
Request a quote for more information about this service.
Interferometry
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
Purification Services
Price on request
Request a quote for more information about this service.
Centrifugation
Price on request
Request a quote for more information about this service.
Imaging & Spectroscopy
Price on request
Request a quote for more information about this service.
Physical Analysis Methods
Price on request
Request a quote for more information about this service.
Biomolecular Interaction Analysis
Price on request
Request a quote for more information about this service.
Size Exclusion Chromatography (SEC)
Price on request
Request a quote for more information about this service.
Chemistry and Materials
Price on request
Request a quote for more information about this service.
Engineering
Price on request
Request a quote for more information about this service.
Analytical Chemistry Services
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.

Biophysical Analysis Lab has not received any ratings.

Be the first to endorse Biophysical Analysis Lab

Endorse this lab