BioInfoExperts laboratory services focuses on the genetic analysis of viral and bacterial pathogens, and can perform whole genome sequencing, ultradeep targeted sequencing, transcriptome sequencing, as well as other pathogen-specific protocols like single genome sequencing.
Our staff is skilled in data generation and assembly using both Illumina and PacBio sequencing platforms, with off-the-shelf kits like Nextera and TruSeq, as well as developing optimized protocols for special projects when premade kits are not adequate. We have sequenced many different viral and bacterial pathogens, with extensive work in the HIV field. Other pathogens include the viruses Hepatitis C, Zika, rotavirus, norovirus and influenza, as well as bacteria like MRSA, cholera, tuberculosis and salmonella.
We will work closely with you and your team to produce high-quality accurate sequencing in an efficient and affordable manner. Our bioinformatics team will be there to assist once the sequencing is finished to assemble genomes, extract informative polymorphisms for phylogenetic analysis, perform clustering analysis on your deep sequencing data, produce a concise list of dysregulated genes for transcriptomics, among other options depending on the specific goals of your project.
We have an extensive publication history, and work closely with our clients and collaborators from the initial planning of experiments until publication of the data. We also provide grant writing and manuscript development services, and we pride ourselves in forming long lasting collaborations with groups around the world. Please contact us today so we can discuss your project and help you get the data you need to move your research forward.
BioInfoExperts utilizes the new Pacific Biosciences Sequel System. Sequel SMRT Cells contain 1 million zero-mode waveguides delivering up to 10 billion bases of long-read sequence per whole-genome analysis run, and up to 20 Gb in RNA isoform sequencing (Iso-Seq). PacBio now supports multiplexing options to reduce costs. With longer reads and multiple passes on the same read (circular consensus sequencing), SMRT sequencing provides enhanced genome reconstruction, ultradeep and accurate targeted sequencing, and novel isoform variant detection.
Sequel System applications:
• Create high-quality whole genome de novo assemblies of eukaryotic organisms
• Survey large population cohort studies for structural variants
• Sequence full-length transcriptomes or targeted transcripts
• Target regions not currently accessible by other technologies
• Detect genomic variation in complex populations on all size scales
• Detect epigenetic modifications
The isoform sequencing (Iso-Seq) application generates full-length cDNA sequences — from the 5’ end of transcripts to the poly-A tail — eliminating the need for transcriptome reconstruction using isoform-inference algorithms. The PacBio Sequel system increases the output for each SMRT cell and eliminates the need for separate size-selected library preparations. Both advances significantly reduce cost. Cost estimation will be calculated based on the depth of coverage (amount of data) needed and number of samples. Turnaround time for sequencing is typically under 30 days from the receipt of samples; data analysis will take additional time and is directly related to existing annotation for reference genomes.
• Full-length transcript sequencing requires intact, non-degraded full length transcripts to prepare cDNA libraries.
• Total RNA amount is ≥ 10 μg, at a concentration of 200 ng/μl or greater, and must be free of DNA contamination.
• RIN value ≥ 8.0 and pure (OD260/280 ≥ 1.8, OD260/230 ≥ 1.8)
We provide manual extraction services for RNA, miRNA, and DNA from many sample types. This service includes RNA quality control and quantification on both the Qubit 3.0 and Agilent Tapestation. We also can provide cDNA synthesis for quantitative PCR (qPCR) and perform qPCR or droplet-digital PCR (ddPCR) for you. We have extensive experience extracting viral RNA from a variety of sample types. We also prepare high-quality RNA extractions for transcriptomic and RNAseq experiments. Typical turnaround time for RNA extraction is 1-3 business days from receipt of the samples.
We can provide genomic DNA extraction services, with affordable prices and high-quality high-yield results. Prices and turnaround time will depend on your preferred method of extraction and number of samples. Typical turnaround time for gDNA extraction is 1-3 days from receipt of the samples.
Bioinformatics covers a large number of services and if you have a job, it is likely we can handle it.
We have a strong combined background in:
BioInfoExperts focuses on whole genome sequencing of viral and bacterial pathogens utilizing the Illumina MiSeq and NextSeq platforms, as well as the PacBio Sequel system. We provide various library preparation services, including off-the-shelf kits and custom-designed protocols as needed. Turnaround times vary with your project requirements. As an example, a 2x300bp PE miSeq v3 run with 24 multiplexed Nextera libraries prepared from supplied bacterial genomic DNA typically is finished with 10 business days from receipt of samples.
DNA (0.1-1ug) should be high-molecular weight and high purity (A260/280:1.8-2.0, A260/230: ≥ 1.8) to obtain optimal results. RNA should have RIN >8.0. QC services are available.
Past sequencing projects have included:
Virus: HIV, HCV, Zika, norovirus, rotavirus and more.
Bacteria: MRSA, salmonella, tuberculosis, V. cholera, and others.
We provide a large range of Nucleic Acids Services including DNA/RNA/miRNA isolation, quantification, purification, imaging, size selection and many more. Please tell us about your project and we will help find the right application to fit your needs.
Biochemistry & Molecular Biology Services
Software Development and IT Services
Cell Culture Media and Reagents Services
Biostatistics & Bioinformatics Services
Next Generation Sequencing (NGS) Services
DNA Extraction and Purification Services
"Great communication, quick turnover time, high quality results."
BioInfoExperts LLC has not received any endorsements.