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Biofizik, Inc.

San Diego, California, US

Biofizik, Inc. is a data generating contract research organization specializing in biophysical approaches for drug discovery and development and the basic life sciences.

Biofizik, Inc. was formed in 2016 by Simon Bergqvist as a resource for biophysical binding approaches to enable small molecule and biotherapeutic drug discover & development and life sciences basic research.

Prior to Biofizik, Inc. Simon led the biophysics group at the Pfizer, La Jolla Oncology Research Unit. This previous role supported a portfolio of drug discovery and development programs for small molecule and biotherapeutic drugs.

Biofizik, Inc. has capabilities for small molecule and macromolecule binding studies with a particular emphasis on understanding the thermodynamic and kinetic underpinnings of binding interactions and applying them to support both drug discovery & development and basic science... Show more »

Biofizik, Inc. is a data generating contract research organization specializing in biophysical approaches for drug discovery and development and the basic life sciences.

Biofizik, Inc. was formed in 2016 by Simon Bergqvist as a resource for biophysical binding approaches to enable small molecule and biotherapeutic drug discover & development and life sciences basic research.

Prior to Biofizik, Inc. Simon led the biophysics group at the Pfizer, La Jolla Oncology Research Unit. This previous role supported a portfolio of drug discovery and development programs for small molecule and biotherapeutic drugs.

Biofizik, Inc. has capabilities for small molecule and macromolecule binding studies with a particular emphasis on understanding the thermodynamic and kinetic underpinnings of binding interactions and applying them to support both drug discovery & development and basic science research.

Publications

  • Spectrum and Degree of CDK Drug Interactions Predicts Clinical Performance. Chen, P., Lee, N., Hu, W., Xu, M., Ferre, P.A., Lam, H., Bergqvist, S., Solowiej, J., et al. Mol. Cancer Ther. 2016. Oct; 15 (10):2273-2281.
  • Design and Synthesis of Pyridone Containing 3,4-Dihydroisoquinoline-1(2H)-ones as a Novel Class of Enhancer of Zeste Homolog 2 (EZH2) Inhibitors. Kung, P.; Rui, E., Bergqvist, S., Bingham, P., Braganza, J., Collins, M., Cui, M., et al. J. Med. Chem. 2016 Sep 22; 59 (18) 8306-25.
  • Resensitization to Crizotinib by the Lorlatinib ALK Resistance Mutation L1198F. Shaw, A.T., Friboulet, L., Leshchiner, I., Gainor, J.F., Bergqvist, S., Brooun, A., et al. N Engl J Med. 2016 Jan 7;374(1):54-61.
  • Engineering of an isolated p110 subunit of PI3K permits crystallization and provides a platform for structure-based drug design. Chen, P., Deng, Y.L., Bergqvist, S., Falk, M., Liu, W., Timofeevski, S., Brooun, A. et al. Protein Sci. 2014 23 (10), 1332-40.
  • Covalent EGFR inhibitor analysis reveals importance of reversible interactions to potency and mechanisms of drug resistance. Schwartz, P.A., Kuzmic, P., Solowiej, J., Bergqvist, S., Bolonos, B., Almaden, C., Nagata, A., Ryan, K., Feng, J., Dalvie, D., Kath, J.C., Xu, M., Wani, R.; Murray, B.W. PNAS USA. 2014 Jan 7;111(1):173-8.
  • Identification of Cys255 in HIF-1 as a novel site for the development of covalent inhibitors of HIF-1/ARNT PasB domain protein-protein interaction. Cardoso, R., Love, R., Nilsson,C.L, Bergqvist, S., Nowlin, D., Yan, J., Liu, K.C., et al. Protein Sci. 2012. 21 (12), 1885-96.
  • Targeting of 4-1BB by monoclonal antibody PF-05082566 enhances T-cell function and promotes anti-tumour activity. Fisher, T.S, Kamperschroer, C., Oliphant, T., Love, V., Lira, P.D., Doyonnas, R., et al. Cancer Immunol Immunother. 2012. 61 (10), 1721-33.
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Surface Plasmon Resonance (SPR)
Price on request

SPR studies offer relatively high throughput and low sample requirement compared to other direct binding approaches. Assay conditions must be optimized to provide robust capture of the ligand. Part of Biofizik’s assay development offering is to create a robust optimized assay for detection of small or large molecule binding.
A... Show more »

SPR studies offer relatively high throughput and low sample requirement compared to other direct binding approaches. Assay conditions must be optimized to provide robust capture of the ligand. Part of Biofizik’s assay development offering is to create a robust optimized assay for detection of small or large molecule binding.
A great strength of biosensors for binding studies is the wide dynamic range suitable for screening and of early fragment screening hits to late stage pre-clinical compounds with little modification to the basic assay conditions.
Biacore biosensor systems provide a versatile biosensor platform that can be used to study the binding affinity and real time kinetics of a range of biological systems including small-molecule and antibody binding interactions.

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Biacore 3000
Isothermal Titration Calorimetry (ITC)
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ITC is benchmark technique for binding interaction analysis providing a direct solution binding measure of equilibrium binding affinity (KB/KD), binding stoichiometry and enthalpy change (ΔH).
Assay development is very simple for ITC comprising mainly of exchanging the binding partners into a matched buffer system and accurately... Show more »

ITC is benchmark technique for binding interaction analysis providing a direct solution binding measure of equilibrium binding affinity (KB/KD), binding stoichiometry and enthalpy change (ΔH).
Assay development is very simple for ITC comprising mainly of exchanging the binding partners into a matched buffer system and accurately determining the sample stock concentrations.
Concentration determination is typically performed spectrophotometrically using the NanoDrop system for proteins and by accurate weighting of small molecule compounds.
The typical dynamic range of KD determination for ITC studies is 10e-5 - 10e-8 nM. However, this can be extended through modifications to the standard procedures.

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Differential Scanning Calorimetry (DSC)
Price on request

DSC is a routine assay for determination of unfolding properties of a protein. DSC can be run on either an automated capillary DSC system or manually using a larger volume cell where greater sensitivity is required. DSC provides ranking of compound binding to proteins by the accurate measurement of the shift in unfolding temperature.

DSC is a routine assay for determination of unfolding properties of a protein. DSC can be run on either an automated capillary DSC system or manually using a larger volume cell where greater sensitivity is required. DSC provides ranking of compound binding to proteins by the accurate measurement of the shift in unfolding temperature.

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Fragment Screening
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Data Inspection and Preparation
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Calorimetry
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Data Services
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Computational Modeling
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Biology
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Physical Analysis Methods
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Biomolecular Interaction Analysis
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Engineering
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Hit Identification
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Drug Discovery & Development
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Biochemistry & Molecular Biology
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Drug Discovery
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December 23, 2017

Positive review received for Isothermal Titration Calorimetry (ITC):

"Working with Simon is like having another team member. This project we set up required flexibility as we did not know what to expect from drug binding. Simon set up our project to be evaluated at specific milestones and we did not waste time or money. He is responsive, communicative, and very knowledgeable. I already have ideas for our next project!"

2017-12-19 04:35:38 +0800

Net Promoter Score of 10 received for Isothermal Titration Calorimetry (ITC).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 9.
2017-11-30 09:45:20 +0800

Net Promoter Score of 10 received for Isothermal Titration Calorimetry (ITC).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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