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Bioexpression and Fermentation Facility

Athens, Georgia, US

We are a core facility at the University of Georgia, and we have been active since 1967. We offer protein production, monoclonal antibody and cell culture services. We operate under the ICH Q10 quality system.

Instrumentation: 23 fermenters (1L - 800L scale), GC / HPLC, FPLC

Available animal models: mice, rats, goats, rabbits

Available cell lines: Hybridoma, HEK, CHO, other mammalian lines

Available fermentation: E. coli, Pichia, yeast, other bacteria and archaea

Bioexpression and Fermentation Facility has not listed any services.

Animal Immunization for Antibody Production
Price on request
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Rabbit
Goat
Mouse
Rat
Site-Directed Mutagenesis
Price on request

Instrumentation: We use a PCR based method for reactions and provide Sanger sequence QC data for all mutants
Sample requirements: 500 ng of DNA
Turnaround time: 2 weeks from start of project (projects start at a set time each week)
Deliverables: plasmid DNA (dried) and electronic sequence QC data

Instrumentation: We use a PCR based method for reactions and provide Sanger sequence QC data for all mutants
Sample requirements: 500 ng of DNA
Turnaround time: 2 weeks from start of project (projects start at a set time each week)
Deliverables: plasmid DNA (dried) and electronic sequence QC data

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Eppendorf Thermalcycler
Bacterial Culture
Price on request

Batch or fed-batch fermentation services. We have 23 bioreactors including 1L (8), 5L (8), 20L (2), 100L (1), 200L (1), 400L(1), 500L(1) and 750L(1)

Available fermentation: E. coli, Pichia

Turnaround time: 2 days, with 2-3 weeks lead time

Standard sample requirements: bacterial strain

Batch or fed-batch fermentation services. We have 23 bioreactors including 1L (8), 5L (8), 20L (2), 100L (1), 200L (1), 400L(1), 500L(1) and 750L(1)

Available fermentation: E. coli, Pichia

Turnaround time: 2 days, with 2-3 weeks lead time

Standard sample requirements: bacterial strain

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Microbial Fermentation and Bioprocessing
Price on request

Batch or fed-batch fermentation services. We have 23 bioreactors including 1L (8), 5L (8), 20L (2), 100L (1), 200L (1), 400L(1), 500L(1) and 750L(1)

Available cell lines: myeloma lines, AGK, CHO, Bacculi virus

Turnaround time: 2 days, with 2-3 weeks lead time

Standard sample requirements: strain

Batch or fed-batch fermentation services. We have 23 bioreactors including 1L (8), 5L (8), 20L (2), 100L (1), 200L (1), 400L(1), 500L(1) and 750L(1)

Available cell lines: myeloma lines, AGK, CHO, Bacculi virus

Turnaround time: 2 days, with 2-3 weeks lead time

Standard sample requirements: strain

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Gas Chromatography (GC)
Gas Chromatography
Price on request

Automated sample analysis with capability to detect a variety of products. Please inquire.

Automated sample analysis with capability to detect a variety of products. Please inquire.

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HPLC
High Performance Liquid Chromatography
Price on request

Analytical services including reverse phase, ion exchange and size exclusion

Analytical services including reverse phase, ion exchange and size exclusion

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Mammalian Cell Culture
Price on request

Production of CHO, HEK, Baculovirus or Hybridoma cell lines. We have 3 bioreactors at 1L, 10L and 25L scales.

Turnaround time: 2 days, with 2-3 weeks lead time

Standard sample requirements: strain

Production of CHO, HEK, Baculovirus or Hybridoma cell lines. We have 3 bioreactors at 1L, 10L and 25L scales.

Turnaround time: 2 days, with 2-3 weeks lead time

Standard sample requirements: strain

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Peptide Synthesis
Price on request

We offer peptide production services using a proprietary technology. Staff are experienced with production of difficult peptide targets.

-Recombinant production of peptides >30 amino acids in length
-Process development to gram scale production
-Fill/finish lyophilization in vials available

We offer peptide production services using a proprietary technology. Staff are experienced with production of difficult peptide targets.

-Recombinant production of peptides >30 amino acids in length
-Process development to gram scale production
-Fill/finish lyophilization in vials available

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Fermentation Process Optimization
Price on request

Fermentation optimization to improve the expression of a gene of interest. Fermentations run in banks of 4. Conditions such as temperature, pH, media, dissolved oxygen and feeds can be evaluated in an iterative fashion or using Design of Experiments (DOE).

Fermentation optimization to improve the expression of a gene of interest. Fermentations run in banks of 4. Conditions such as temperature, pH, media, dissolved oxygen and feeds can be evaluated in an iterative fashion or using Design of Experiments (DOE).

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Protein Purification and Quantification
Price on request

We offer purification services utilizing a variety of chromatography types including: Affinity, Ion Exchange, Size Exclusion and Hydrophobic Interactions. Our scale is from 1mL - 10L packed bed columns. We offer diafiltration and concentration via TFF up to 10 m2 membranes with a variety of membrane sizes and types.

Standard deliverable: report

We offer purification services utilizing a variety of chromatography types including: Affinity, Ion Exchange, Size Exclusion and Hydrophobic Interactions. Our scale is from 1mL - 10L packed bed columns. We offer diafiltration and concentration via TFF up to 10 m2 membranes with a variety of membrane sizes and types.

Standard deliverable: report

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Western Blot
Price on request
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Yeast Culture
Price on request

BFF has experience with a wide range of organisms including bacteria, yeast, mycelial fungi, cell culture. Fully controlled fermenters are available and offer the following benefits:

-Batch, fed-batch, or continuous culture
-Wide range of volumes available: 5L, 20L, 100L, 250L, 400L, 500L, 800L working volumes
-Oxygen... Show more »

BFF has experience with a wide range of organisms including bacteria, yeast, mycelial fungi, cell culture. Fully controlled fermenters are available and offer the following benefits:

-Batch, fed-batch, or continuous culture
-Wide range of volumes available: 5L, 20L, 100L, 250L, 400L, 500L, 800L working volumes
-Oxygen supplementation and full process control on all fermenters
-All fermenters set up for anaerobic culture and unusual gas mixtures
-High temperature culture for thermophiles and hyperthermophiles available

Turnaround time: 2 days, with 2-3 weeks lead time

Standard sample requirements: strain

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Custom Monoclonal Antibody (mAb) Production
Starting at $3,960.00 per unit

Standard monoclonal antibody project services including immunizations, fusions and production of Hybridoma in cell culture. Five mice to be immunized and the highest titer mouse would be used for a fusion. The project includes immunizations, screening by ELISA, 1 fusion, screening of hybridomas and production of 2L of... Show more »

Standard monoclonal antibody project services including immunizations, fusions and production of Hybridoma in cell culture. Five mice to be immunized and the highest titer mouse would be used for a fusion. The project includes immunizations, screening by ELISA, 1 fusion, screening of hybridomas and production of 2L of supernatant using the best clone selected.

Turnaround time: 6 months once the project initiated

Standard sample requirements: 2-4 mg of immunogen

Standard deliverables: purified antibody from 2L of culture

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Analytical Chemistry Services
Price on request
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Chemistry and Materials
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Purification Services
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Synthetic Chemistry
Price on request
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Chromatography
Price on request
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Liquid Chromatography
Price on request
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Cells and Tissues
Price on request
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Biology
Price on request
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Protein Services
Price on request
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Mutagenesis
Price on request
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Protein Expression Visualization
Price on request
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Antibody Services
Price on request
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Biochemistry & Molecular Biology
Price on request
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2018-01-08 12:43:26 -0800

Net Promoter Score of 10 received for HPLC.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-08-24 07:19:24 -0700

Net Promoter Score of 10 received for Microbial Fermentation and Bioprocessing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
July 21, 2017

Positive review received for Microbial Fermentation and Bioprocessing:

"Efficient! Easy to work with them, especially Ron."

2017-05-15 15:31:48 -0700

Net Promoter Score of 10 received for Yeast Culture.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
May 12, 2017

Positive review received for Mammalian Cell Culture:

"The Bioexpression and Fermentation Facility at UGA was a very great partner in our research efforts. They grew the cells exactly to protocol and produced beautiful, accompanying Gel and Blot data for our review. They are fast, efficient, and always happy to answer any questions we may have along the way. I will continue to use the Bioexpression and Fermentation Facility at UGA for my work moving forward."

April 29, 2017

Positive review received for Yeast Culture:

"Excellent service"

July 12, 2016

Positive review received for Yeast Culture:

"We once again requested BFF to ferment some cells for us and the service has worked just like the first time, quite fast, efficient and profissional. Great job from BFF team!"

March 2, 2016

Positive review received for Microbial Fermentation and Bioprocessing:

"Among few options, I am very glad I decided to work with these guys at "Bioexpression and Fermentation Facility". The negotiation is always very smooth, the quality of the service and their commitment is memorable. We have already requested two services with them and we look forward to placing more orders ahead. We are really happy with the service provided by this facility!"

April 7, 2015

Positive review received for Gas Chromatography (GC):

"We had a great experience with Bioexpression and Fermentation Facility analyzing our samples by GC-FID. The results were delivered in a timely manner with a detailed report. I do recommend their service for chromatography analysis (GC, HPLC)."

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