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Biocytogen LLC.

Worcester, Massachusetts, US

Biocytogen was founded in June 2008, in the Commonwealth of Massachusetts, and Beijing Biocytogen Co., Ltd was established in November 2009. By the end of 2015, Biocytogen has more than 160 employees, and this number is expected to reach 300 by the end of 2016. Biocytogen currently has three subsidiaries located in Worcester, Massachusetts, Beijing Daxing Bio-medicine Industry Park, and Jiangsu Haimen Biotech and Pharmaceutical Park.

During Biocytogen’s 8 years of development, it has rapidly integrated the various advantageous resources of China and United States.Biocytogen has built a development and service platform for genetic knockout mouse based on the following techniques: “unique gene targeting vector construction,” “stable passaged C57BL/6 mouse embryonic stem cells”, “accurate and efficient microinjection” as well as a development and service platform for gene knockout and knockin model animals based on... Show more »

Biocytogen was founded in June 2008, in the Commonwealth of Massachusetts, and Beijing Biocytogen Co., Ltd was established in November 2009. By the end of 2015, Biocytogen has more than 160 employees, and this number is expected to reach 300 by the end of 2016. Biocytogen currently has three subsidiaries located in Worcester, Massachusetts, Beijing Daxing Bio-medicine Industry Park, and Jiangsu Haimen Biotech and Pharmaceutical Park.

During Biocytogen’s 8 years of development, it has rapidly integrated the various advantageous resources of China and United States.Biocytogen has built a development and service platform for genetic knockout mouse based on the following techniques: “unique gene targeting vector construction,” “stable passaged C57BL/6 mouse embryonic stem cells”, “accurate and efficient microinjection” as well as a development and service platform for gene knockout and knockin model animals based on CRISPR/Cas9,TALEN, and EGE(Extreme Genome Editing System). All of these technology platforms have promoted Biocytogen’s rapid development and helped the company become a prestigious model animal development technology service supplier in China and all over the world. Relying on their model organism development platform, Biocytogen has developed or is developing nearly 1000 genetic knockout/knockin mouse strains, more than 100 genetic knockout/knockin rat strains, more than 200 genetic knockout/knockin cell lines, and, in cooperation with research institutes, 4 primate strains.

Selected Publications

  • Zhou S, Liu Q, Wu X, Chen P, Wu X, Guo Y, Liu S, Liang Z, Fan C, Wang Y. A safe and sensitive enterovirus A71 infection model based on human SCARB2 knock-in mice. Vaccine. 2016 May 23;34(24):2729-36. doi: 10.1016/j.vaccine.2016.04.029.
  • Wu M, Wei C, Lian Z, Liu R, Zhu C, Wang H, Cao J, Shen Y, Zhao F, Zhang L, Mu Z, Wang Y, Wang X, Du L, Wang C. Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system. Sci Rep. 2016 Apr 11;6:24360. doi: 10.1038/srep24360.
  • Liu Q, Fan C, Zhou S, Guo Y, Zuo Q, Ma J, Liu S, Wu X, Peng Z, Fan T, Guo C, Shen Y, Huang W, Li B, He Z, Wang Y. Bioluminescent imaging of vaccinia virus infection in immunocompetent and immunodeficient rats as a model for human smallpox. Sci Rep. 2015 Aug 3;5:11397. doi: 10.1038/srep11397.
  • Gopinathan G, Jin T, Liu M, Li S, Atsawasuwan P, Galang MT, Allen M, Luan X, Diekwisch TG. The expanded amelogenin polyproline region preferentially binds to apatite versus carbonate and promotes apatite crystal elongation. Front Physiol. 2014 Nov 11;5:430. doi: 10.3389/fphys.2014.00430.
  • Zhu G, Li Y, Zhu F, Wang T, Jin W, Mu W, Lin W, Tan W, Li W, Street RC, Peng S, Zhang J, Feng Y, Warren ST, Sun Q, Jin P, Chen D. Coordination of engineered factors with TET1/2 promotes early-stage epigenetic modification during somatic cell reprogramming. Stem Cell Reports. 2014 Feb 27;2(3):253-61. doi: 10.1016/j.stemcr.2014.01.012.
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Biocytogen LLC. has not listed any services.

Cell Line Derived Xenograft (CDX) Models
Cell Line Derived Xenograft Models
Price on request

Pharmacological and pharmacodynamic test for CDX (Cell line-Derived Xenograft) tumor model

CDX (Cell line-Derived Xenograft) is a well established model for the research and development of tumor drugs. The model is easy to establish and has excellent repeatability, so it is still under high demand for the research and... Show more »

Pharmacological and pharmacodynamic test for CDX (Cell line-Derived Xenograft) tumor model

CDX (Cell line-Derived Xenograft) is a well established model for the research and development of tumor drugs. The model is easy to establish and has excellent repeatability, so it is still under high demand for the research and development of tumor drugs. Cell line models are established by inoculating passage tumor cells in vitro, and culturing them into the body of immune deficient mice, which have high homology[What has high homology? The cells or the immune deficient mice? I assume the mice so I have changed accordingly] due to the long-term in vitro passage of cells.

Available services: Provide various cell line tumor models according to the customer’s requirements for research and development; establishment of subcutaneous tumor model and orthotopic tumor model or metastatic tumor model; provide in vivo pharmacological and pharmacodynamic service for the corresponding models.

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TALEN Genome Editing
Price on request

Brief introduction of the technology

TALEN (Transcription Activator-Like Effector Nuclease) is another high-efficiency genome modification tool. Its high efficiency, strong specificity, low missing rate, and simple design makes the technology widely used for gene knockout and genome modification at the cell and in vivo... Show more »

Brief introduction of the technology

TALEN (Transcription Activator-Like Effector Nuclease) is another high-efficiency genome modification tool. Its high efficiency, strong specificity, low missing rate, and simple design makes the technology widely used for gene knockout and genome modification at the cell and in vivo levels.

Service process

BIOCYTOGEN provides services for mouse model construction in one step, including model designing, construction, and genotypic identification service

  • Project feasibility evaluation and model design

BIOCYTOGEN possesses a senior technical team specializing in comprehensive analysis of project feasibility, and provides a free and professional model design service. The services include:

Ÿ Define the purpose for constructing the model
Ÿ Analyze the target gene’s structure and sequence information
Ÿ Analyze existing data
Ÿ Design all possible model construction schemes
Ÿ Analyze the advantages and risks of all model construction schemes
Ÿ Accurate prediction of project implementation time and quotation

  • Design and construction of TALEN

  • Pronuclear injection

Ÿ Pronuclear injection of the mRNA (TALEN) into the mouse zygotes

  • Obtain the mouse of generation F0 and breeding

Ÿ Recipient female mouse feeding and the birth of chimeric mouse
Ÿ Mating of chimeric mouse and wild mouse

  • Obtain the phyla-genetic hybrid mouse of generation F1

Ÿ Birth of generation F1 mice
Ÿ Ensure the phyla heredity by identification of generation F1 mouse tail genotype

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Humanized Mouse Models
Price on request

Definition:

A mouse model with a functional human gene, cell, tissue, or organ, which is used as living body substitution model for human disease study. This service refers to the model with the functional human gene.

Advantages and disadvantages:

Advantages: the human gene is expressed in the mouse body using the... Show more »

Definition:

A mouse model with a functional human gene, cell, tissue, or organ, which is used as living body substitution model for human disease study. This service refers to the model with the functional human gene.

Advantages and disadvantages:

Advantages: the human gene is expressed in the mouse body using the promoter and regulation region of the mouse gene.

Disadvantages: to ensure that the human gene is physiologically expressed and regulated normally in the mouse genome, the sequence and structure information of the genes and proteins should be analyzed in depth before designing, and comprehensive risk assessments should be built.

Principle

A humanized mouse model with a functional human gene is constructed by replacing part or all of the mouse gene with the human gene. The expression of the human protein in the mouse body is the same as the mouse protein’s expression, but the protein or functional region(s) of the mouse protein is not expressed in any cells or tissues.

Application

  • Human gene expression and functional studies
  • Human disease studies, such as AIDS, cancer, infectious diseases, human degenerative diseases, and blood diseases, etc.
  • Preclinical drug study
  • Compound safety evaluation
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Knockout Mouse Development
Price on request

Definition:

Use the homologous recombination technology to replace all the exons, several important exons, or function domains of a target gene using a section of exogenous DNA sequence, and obtain a mouse model where all of the tissues and cells during all developmental stages (from 1-cell embryo to adult stage) do not... Show more »

Definition:

Use the homologous recombination technology to replace all the exons, several important exons, or function domains of a target gene using a section of exogenous DNA sequence, and obtain a mouse model where all of the tissues and cells during all developmental stages (from 1-cell embryo to adult stage) do not express the gene.

Advantages and disadvantages:

  • Advantages: the design of the targeting strategy and construction of the target vector is simple
  • Disadvantages: the knockout of the target gene may cause embryonic death or expression dysregulation of other genes. The conditional knockout design strategy can be used if this risk is predicted to exist.

Application:

  • Physiological or pathological function study of a gene on the whole-body level
  • Drug target affirmation
  • Drug toxicological study

Principle:

The conventional knockout realizes the target gene knockout by replacing one or more exons of the target gene using the screening gene neomycin (Neo). The knockout occurs in all of the tissues and cells in the body, which includes the risk of embryonic death. This risk can be avoided by a conditional knockout strategy.

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Embryonic Stem Cell Gene Targeting
Price on request
  • Rosa26 locus gene knockin mouse model
  • Reporter/tag mouse model
  • Conditional point mutation mouse model-tissue specific
  • Conditional point mutation mouse model-induced
  • Conventional point mutation mouse model
  • Humanized mouse model
  • Knockin mouse model
  • Knockout first, conditional ready mouse model
  • Conventional... Show more »
  • Rosa26 locus gene knockin mouse model
  • Reporter/tag mouse model
  • Conditional point mutation mouse model-tissue specific
  • Conditional point mutation mouse model-induced
  • Conventional point mutation mouse model
  • Humanized mouse model
  • Knockin mouse model
  • Knockout first, conditional ready mouse model
  • Conventional knockout mouse model
  • Gene Targeting Technology Based on Embryonic Stem Cell (ESC/HR)
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Knock-In Mouse Development
Price on request

Definition: mouse models with single or multiple base changes or an exogenous gene introduced into the target gene position.

Principle: Knockins introduce specific mutation(s) or an exogenous gene in the target gene position. For example, this method can introduce point mutation(s), which can simulate a human genetic disease,... Show more »

Definition: mouse models with single or multiple base changes or an exogenous gene introduced into the target gene position.

Principle: Knockins introduce specific mutation(s) or an exogenous gene in the target gene position. For example, this method can introduce point mutation(s), which can simulate a human genetic disease, in the target gene. It can also be used to introduce a reporter gene (such as EGFP, mRFP, mCherry, mYFP or LacZ, etc.) or functional cDNA (such as Cre and Dre, etc.) to be expressed in a specific locus by homologous recombination, thereby making the expression of the reporter gene or other cDNA accordant with the expression of the target gene. The knockout and knockin occurs at the same time when the reporter gene or cDNA replaces the mouse gene.

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Genome Editing
Price on request

Using the EGE system, EGFPand mCherrygenes are knocked into the ACTB and LMNB1 translation initiation sites, respectively, in the rodent C6 cell lines.

By using flow cytometry analysis, we have found that the CRISPR/Cas9 mediated gene EGFP-ACTB knockin in efficiency is only 1.91% in U2OS cells while the efficiency is 15.02% by... Show more »

Using the EGE system, EGFPand mCherrygenes are knocked into the ACTB and LMNB1 translation initiation sites, respectively, in the rodent C6 cell lines.

By using flow cytometry analysis, we have found that the CRISPR/Cas9 mediated gene EGFP-ACTB knockin in efficiency is only 1.91% in U2OS cells while the efficiency is 15.02% by using EGE system, which shows an 8-fold increase. In C6 cell line, the EGFP-LMNB1 knockin efficiency increased from 0.19% to 3.6% by using EGE system, which is about a 19-fold increase.

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Vector Construction and Optimization
Price on request

Targeting Vector Construction Services

  1. ES Cell based targeting vector: (1-2 months) Including analysis of gene structure, screening strategy design, targeting vector construction and confirmation (DNA sequencing verification). Southern blot strategy and probe design will also be provided.
  2. CRISPR/Cas9 based sgRNA/Cas9... Show more »

Targeting Vector Construction Services

  1. ES Cell based targeting vector: (1-2 months) Including analysis of gene structure, screening strategy design, targeting vector construction and confirmation (DNA sequencing verification). Southern blot strategy and probe design will also be provided.
  2. CRISPR/Cas9 based sgRNA/Cas9 vector (1-2 months): Including analysis of gene structure, screening strategy design; construction and confirmation of CRISPR/Cas9 plasmids, sgRNA activity testing (selected the best activity one among 4-6 sgRNAs)
  3. CRISPR/Cas9 based donor plasmid (DNA template for cKO/KI): (1-2 months) Including analysis of gene structure, screening strategy design; targeting vector construction and confirmation (DNA sequencing verification)
  4. Sub-cloning plasmid: (2-3 weeks)
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Transgenic Rat Development
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Custom Mouse Models
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Prostate Cancer Animal Models
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Animal Models and Studies
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Animal Models of Disease
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Oncology Animal Models
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Xenograft Models
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Model Organisms
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Mammalian Models
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Rodent Models
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Mouse Models
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Biology
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Cells and Tissues
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In vitro Disease Models
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Genetic Engineering
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Transgenic Animal Model Development
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Transgenic Mouse Services
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Services
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Plasmids and Vectors
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2018-05-24 03:05:13 -0700

Net Promoter Score of 9 received for Humanized Mouse Models.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-01-09 07:28:33 -0800

Net Promoter Score of 9 received for Humanized Mouse Models.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 7.

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