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Bioalternatives

12 Orders Completed
Gençay, FR

About Bioalternatives

Founded: 1996 Type: Partnership Size: 11-50 employees

From in silico testing to the clinic, Bioalternatives provides a global offer in the world of testing.


As your partner in innovation, Bioalternatives supports you at each step of your project and offers a global outsourcing solution, from in silico testing to the bioanalysis of non-invasive clinical samples, including in vitro and ex vivo testing and an exclusive offer based on a fresh blood model: "Bloodassaysolutions".


Our know-how is based on laboratories specialized in cellular and molecular pharmacology, biochemistry, microbiology, analytical chemistry and tissue engineering.


As a Contract Research Organization (CRO), Bioalternatives proposes a unique offer in dermo-cosmetics and life sciences research and has a strong expertise in the evaluation of active ingredients and product efficacy (drugs, cosmetics, medical devices, food supplements) in the fields of skin biology, immune-inflammation, neurobiology and veterinary medicine.


Would you like to analyze your biological samples, evaluate the efficacy of your compounds or develop customized tools, models and methods? Contact us!

    Our Services (47)


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    Cosmetic and Personal Care Product Testing

    Price on request

    In vitro and ex vivo efficacy studies of cosmetic products: oily skin, dry skin, hydration, skin ageing, pigmentation, hair growth, etc.


    You are looking for validated or innovative efficacy assays to support your cosmetic claims? You wish to characterize your active ingredients, demonstrate the efficacy of your cosmetic formulations? Or would you like to test the safety of your products at an early stage? Our services will help you meet your objectives and support your projects.


    With our strong expertise in numerous in vitro and ex vivo models and in skin physiology, our aim is to bring value to your cosmetic products:
    - Screening of bioactive ingredients
    - Mechanism of action and proof-of-concept
    - in vitro safety assays (non-GLP)
    - Repositioning or benchmarking studies
    - in vitro or ex vivo evaluation of cosmetic formulations before clinical trials.


    As your partner in innovation, we provide you with new concepts to substantiate your claims
    Let’s talk about it!

    Biology Cosmetics

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    In vitro Skin Models

    Price on request
    We provide a wide range validated assays for compound (ingredient or finish products) testing using:
    -2D skin models
    Normal human epidermal keratinocytes (NHEK)
    Normal human dermal fibroblasts (NHDF)
    Normal human epidermal melanocytes (NHEM)
    Human sebocytes SEBO662 and SEBO662AR cell lines
    etc.
    -3D-skin related models
    dermis equivalent, (DE)
    reconstructed human epidermis (RHE)
    reconstructed human epidermis including melanocytes (RHEm)
    full thickness skin model
    3D sebocytes
    etc.
    -Ex vivo skin models

    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!

    Skin ageing is a natural, multifactorial and still little-known process which results both from intrinsic factors (genomic, hormonal, etc.) and from external aggravating factors (UV radiation, pollution, food, free radicals, etc.). The latter are referred to as accelerated ageing or as photo-ageing in the particular case of UVs.
    Intrinsic ageing and accelerated ageing have slightly different phenotypic characteristics. Intrinsic ageing is characterized by skin dryness, fine wrinkles and a loss of adipose tissue. Photo ageing is characterized by a significant loss of firmness and elasticity (loose skin), deeper wrinkles and pigmentation disorders.
    In the past few years, significant progress has been made in the understanding of cell ageing mechanisms and we can now explore new dermo-cosmetics strategies which might prevent or decelerate the signs of ageing.


    Bioalternatives has many in vitro models at your disposal:
    intrinsic ageing model:
    - “aged” human dermal fibroblasts (Hayflick model)
    - “aged” dermal equivalent (Hayflick model)
    accelerated ageing model:
    - human dermal fibroblasts aged by oxidative stress (H2O2)
    - human reconstructed skin in deficient medium
    photo-ageing model:
    - human dermal fibroblasts subjected to UVA, infrared or UV radiation
    - photo-aged human full thickness reconstructed skin


    Readout:
    - cell renewal (cell proliferation, migration and differentiation)
    - extracellular matrix synthesis and degradation (collagen, elastin, hyaluronic acid, MMPs, etc.)
    - senescence marker expression
    - free radical production


    Example of Bioalternatives assays:
    - Aged fibroblasts, reversion of aging, procollagen I and/or MMP-1 synthesis
    Skin is the largest organ of the human body and the most exposed of external aggressions (chemical, UV, smoke, humidity etc.). All of these disturbances participate in the phenomenon of aging and the consequence is both phenotypic and structural modifications (reduction in the thickness of the skin, loss of cohesion, loss of elasticity, wrinkles, etc.).
    Disturbances caused such as UV radiation cause epidermal stress contributing to the appearance of oxygen reactive species (ROS) capable of inactivating the phosphotase itself responsible for the activation of tyrosine kinase receptors. When activated, these receptors act on the map kinase pathway leading to the inhibition of procollagen synthesis as well as to the synthesis of matrix metalloproteases (MMP). MMPs are enzymes which act on the degradation of the components of the extracellular matrix (collagen, elastin, proteoglycans). This significant increase in MMP activity is the main factor influencing skin aging.
    The purpose of this test is to measure by ELISA the level of expression of procollagen 1, collagen 1, an essential component of the extracellular matrix, and MMP-1 a collagenase protein responsible of the degradation of collagen within the extra cellular matrix, on aged Normal Human Dermal Fibroblasts.
    - Aged fibroblasts, control of aging
    Replicative aging/senescence is induced in fibroblasts by repeated subcultures (15-20 passages) of normal human dermal fibroblasts. This method modifies the metabolic activity and the gene expression profile of the cells generating a profile of aged fibroblasts characterized by:
    - reduced cell proliferation,
    - reduced synthesis of collagens and other ECM components,
    - increased production of ECM degrading enzymes like MMPs,
    - increased beta-galactosidase activity,
    - expression of specific aging markers.
    The purpose of this assay is to evaluate, via RT-qPCR  technology, the potential anti-aging effect of the compound on aged dermal fibroblasts (Hayflick model) versus normal human dermal fibroblasts (NHDF) at the transcriptional level (modulation of gene expression). More specifically, the effects of the compound on aged fibroblasts are analyzed using the RT-qPCR technology. Extracted mRNA is analyzed on a dedicated PCR array (expression of a set of « aging » genes, mQPA-NHDF AGING-16 or 64).
    - Fibroblasts, prevention of aging, procollagen I and/or MMP-1 synthesis (H2O2 stimulation)
    Skin ageing is characterized by dermis changes resulting from complex mechanisms. These mechanisms combine a decrease of the synthesis of extracellular matrix components, particularly collagen I, and an increase of protease expression and/or activity. Proteases such as MMPs (matrix metalloproteinases) are matrix-degrading enzymes.
    There are multiple consequences to these changes: increase of stiffness of the collagen network and decrease of matrix volume, which have both an impact on the biomechanical properties of dermis.
    The purpose of this assay is to evaluate, via an ELISA test, the capacity of compounds (molecules, active ingredients, extracts, etc.) to prevent the decrease of procollagen I production and the increase of MMP-1 (matrix metalloproteinase-1) production in a model of fibroblasts, prematurely aged by H2O2 treatment.
    - And more other tests


    Other additional information (graphics, illustrations) are also provided site on our website
    Contact us!


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    Product Testing Services

    Price on request

    At Bioalternatives, we are committed to developing effective in vitro alternative options to animal experimentation methods, by offering a full range of solutions for the development of active ingredients and cosmetic formulations.
    We offer customized technical solutions to guide your product research and support the claims of your cosmetic products. Our selection of in vitro testing solutions can be used to characterize your cosmetic products’ active ingredients, demonstrate the efficacy of your formulations, and test the safety of your cosmetic products at an early stage (for R&D purposes only).
    With extensive experience in cosmetic product testing and state-of-the-art facilities, we are pleased to offer you dedicated project management support and consulting for your R&D process.



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    Pharmacology

    Price on request

    Bioalternatives provides services to support your research needs in biology and in in vitro pharmacology. For more than 20 years, we have been assisting scientists from the pharmaceutical and biotechnological industry on preclinical issues (exploratory research, in vitro modelling, discovery of therapeutic molecules).
    Because each of our clients is unique and each study is based on different prerequisites and objectives, we focus on the customized technical solution that can best meet your requirements. We pay particular attention to the feasibility study and to the personalized management of your project, especially through regular and open communication between your study manager and our specialists.
    in vitro pharmacological studies are our core business: by managing a plurality of projects, our scientists have acquired an expertise and know-how which can be applied to a wide range of products.


    Pharmaceutical products and active ingredients:
    - Natural extracts (plant, marine, bacterial)
    - Synthetic molecules, peptides, proteins, glycoproteins, lipids
    - Biotherapies and biosimilars (antibodies, growth factors)
    - Formulations (creams, gels, lotions)
    - Medical devices


    in vitro pharmacological studies:
    - 2nd or 3rd intention screening
    - Activity profiling
    - Proof-of-concept, study of mechanism of action
    - Confirmatory assay
    - Biosimilarity

    Pharmaceutical

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    Histological Staining

    Price on request

    Bioalternatives offers histology services.
    As a researcher in the pharmaceutical industry or as a physician, you wish to carry out histological or immunohistochemical analysis as part of your research program or as part of your preclinical or clinical studies.

    Do you have samples (tissues, human or animal biopsies), blocks or slides that are ready to be analyzed? Are you looking for a reactive high quality contract research organization that can provide you with expertise and flexibility?


    Our laboratory and our special team dedicated to histology and immunohistochemistry methods would like to assist you with your studies:


    Routine assays

    • Preparation of samples to study:


          - cryo blocks or paraffin inclusion


          - generation of slices

    • Methods:

          

    • trichrome staining, specific staining

          

    • immunolabeling (immunofluorescence or immunohistochemistry)

          

    • Microscope observation and generation of images

          

    • Image analysis: scoring, quantification


      Customized services

      - Development:

          

    • staining

          

    • immunolabeling (research and validation of antibodies)

          

    • co-labeling

    • Preparation of slices:

          

    • Images, image mosaic, slide scanning, MET/MEB

          

    • Quantification / image analysis (thickness, intensity, counts, etc.)


    Our Histological staining services:

    At Bioalternatives, we offer more than thirty standard (topographic) histological and special (descriptive) staining techniques.


    These staining processes are carried out after a thorough preparation of samples and slides.

    – With routine standard staining techniques, tissue morphology (structure, organization, dimension / nucleus, cytoplasm, collagen fibers) can be examined and tissue integrity or alteration can be evaluated.


    – Special staining techniques allow us to go further in tissue observation and to visualize particular tissue components (lipids, melanin pigments, elastic fibers etc.).

    Some tissue components can be highlighted in situ by using special staining techniques based on biochemical reactions between the dyes and the tissue components.


    Example of Bioalternatives assays:

    • RHE, epidermal morphology (Hematoxylin-Eosin staining):


      The purpose of this test is to observe the effect of compounds on RHE morphology
      After incubation, RHE were rinsed and fixed with a formaldehyde solution. Fixed tissues were dehydrated in multiple baths with increasing concentration of ethanol and then embedded in paraffin. Transversal sections were performed with a microtome.


      The sections were deparaffinized and stained with a solution of hematoxylin. Sections were rinsed and whitened using a diluted hydrogen chloride (HCl) solution, then stained using an eosin solution. After washes, the sections were rinsed, dehydrated and mounted in CV Ultra mounting medium

    • Hair follicle, pigmentation:

      At the end of incubation, the selected hair follicles were rinsed and fixed with formaldehyde solution. Follicles were then dehydrated in multiple baths with increasing concentration of ethanol and then embedded in paraffin. The transversal sections were performed using a microtome and kept at room temperature until staining.
      The sections were deparaffinized, rinsed in acidified water and then incubated in silver nitrate solution. The sections were then washed and incubated in a silver nitrate, gelatin, and hydroquinone. After washing, the sections were incubated in a sodium thiosulfate solution. After washing, the sections were counter-stained with a Fast-red solution. Finally, the sections were washed again and mounted in CV Ultra medium.

    • 3D sebocytes, lipid accumulation:

      Sebocyte differentiation is associated to an increase of lipid synthesis and accumulation. BIOalternatives has developed a human cell line (SEBO662) able to spontaneously differentiate into a sebaceous-like phenotype when cultured as a three-dimension (3D) epithelium.

    • And more other tests


    Other additional information (graphics, illustrations) are also provided on our website.


    Contact us!

    Leica microtome Microscopes Biology Biomarker analysis 3D Tissue Culture histology Special staining IHC Staining immunostaining 3D cell models Skin Human Dermatology Show 13 more tags Show less

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    Immunostaining

    Price on request

    Bioalternatives offers immunolabeling services in order to detect the cell and tissue components in a tissue section. Proteins of interest (antigens) are detected by using polyclonal or monoclonal antibodies specifically directed against the target proteins.

    Our laboratory can perform immunolabeling by using either the direct approach (antibody directly coupled to a marker) or the indirect approach (unlabeled primary antibody specifically recognized by a marker-labeled secondary antibody) for immunolabeling on different types of tissues: reconstructed tissues, human or animal explants and biopsies, paraffin embedded (FFPE) or frozen tissues.

    The detection methods directly depend on the type of marker that is used. Our laboratory uses special in situ detection techniques: immunofluorescence and immunohistochemistry.


    • Immunofluorescence is a technique which consists in detecting and localizing a protein of interest using a specific antibody (directly linked to a fluorophore or detected by a secondary fluorescent antibody). Immunolabeling of proteins of interest is generally associated to nuclear labeling by Hoechst staining or by propidium iodide staining. This technique, when associated to an image processing program (e.g. ImageJ), can also generate fluorescence intensity data that can be analyzed quantitatively. It is also possible to perform co-labeling with this technique.

    • Immunohistochemistry by enzyme detection is the second special in situ detection technique, which consists in detecting and localizing a protein of interest within a tissue section, using a specific antibody which will be detected by an enzymatic reaction (such as peroxidase) that generates a red precipitate from an AEC-type chromogen (Aminoethyl carbazole).


    Our laboratory has validated more than 120 markers and is at your disposal for any immunolabeling request
    This is a non-exhaustive list of the validated biomarkers we offer:


    Inflammation

    • IL-1

    • IL-8

    • PGE2

    Hydration and skin barrier
    - Pro-collagen type I

    - Collagen type I

    - Collagen type III

    - Collagen type VII

    - Collagen IV

    - Hyaluronic Acid

    - Filaggrin

    - E-cadhérine,Epithelial

    Pigmentation

    - Tyrosinase

    Prolifération

    - Ki67

    Differentiation

    - Cytokeratin-10

    - Claudin 1

    - Transglutaminase 1

    Apoptosis
    - Caspase 14

    And more other markers


    Other additional information (graphics, illustrations) are also provided on our website

    Contact us!
     


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    Skin Hydration Measurement

    Price on request

    Hydration and Skin Barrier


    Skin hydration partly determines the aspect of the skin (dry, rough and oily skin, etc.). Through its barrier function, skin is our shield from external stress factors and prevents the evaporation of such essential elements as water, ions and amino acids.
    Skin hydration is mostly linked to the skin barrier function. The role of a moisturizing skin care product is to act on skin surface to physically limit water loss (occlusive effect) or to act at the cellular level via different biological action models:
    - reinforcement of the barrier function and of epidermal differentiation
    - restoration of skin lipids
    - stimulation of hygroscopic endogenous factors (water retention)
    - improvement of cellular exchanges in solutes and in water
    Hydration is a multifactorial process that cannot be understood through a single assay or model. It is therefore more conclusive to use a panel of complementary efficacy assays in order to study and demonstrate the hydrating properties of an active compound or cosmetic product.


    Bioalternatives has many in vitro or ex vivo models at your disposal:
    - normal human epidermal keratinocytes (NHEK)
    - reconstructed human epidermis (RHE)
    - sebocytes (SEBO662AR)
    - full thickness skin (FTSK)
    - skin explants (ex vivo)


    On which we can evaluate case by case the hydrating effect of active compounds or formulations by measuring:
    - the reinforcement of the barrier function
    - the stimulation of the epidermal differentiation (e.g. filaggrin, involucrin, transglutaminase, cytokeratins)
    - lipid synthesis (acid mantle):
        - sebaceous lipids
        - epidermal lipids (e.g. ceramides, cerebrosides and phospholipids)
    - the expression or synthesis of epidermal extracellular matrix components:
        - glycosaminoglycans and hyaluronic acid
        - proteoglycans and ECM receptors
        - proteases (e.g. MMPs)
    - the expression of markers of epidermal cohesion and intercellular cell junctions:
        - occluding junctions and attachment proteins (claudin, occludin, desmogleins, etc.)
        - dermoepidermal junction (e.g. integrin V, collagen IV, collagen VII, etc.)
        - gap junctions (e.g. connexins) and molecular channels (e.g. aquaporins)


    Example of Bioalternatives assays:
    - RHE, transepidermal permeation and barrier function
    The epidermis provides a physical and permeability barrier which is continuously regenerated by the epidermal keratinocyte differentiation process. Multiple components can impact the barrier function quality including protein structure (claudin, desmoglein, filaggrin, etc.), lipids (ceramides) and proteases. When properly differentiated, the epidermis prevents water loss and provides a barrier to epidermal invasion of pollutants, toxins, allergens and bacteria.
    The purpose of our assay is to evaluate, via radiodetection, the capacity of compounds (active ingredients or formulations) to reinforce the human reconstructed epidermis barrier function by analyzing caffeine diffusion.
    - NHEK, epidermal barrier markers (mRNA)
    The epidermis provides a physical and permeability barrier which is continuously regenerated by the epidermal keratinocyte differentiation process. Multiple components can impact the barrier function quality including protein structure (claudin, desmoglein, filaggrin, etc.), lipids (ceramides) and proteases. When properly differentiated, the epidermis prevents water loss and provides a barrier to epidermal invasion of pollutants, toxins, allergens and bacteria.
    The purpose of our assay is to evaluate, via RT-qPCR technology, the capacity of the compounds to improve the epidermal barrier by analyzing gene expression level of 32 markers related to keratinocyte differentiation, tight junctions and adherens junctions, hyaluronic acid synthesis and other.
    - NHEK, hyaluronic acid synthesis / release
    Hyaluronic acid, a sulfate-free glycosaminoglycan, is one of the major components of the extracellular matrix. Hyaluronic acid fills the inter-cellular spaces in skin. It plays an important role in maintaining skin hydration and turgescence, due to its capacity to capture water molecules and ions.
    The purpose of our in vitro assay is to evaluate, via an ELISA test, the stimulating effects of compounds (molecules, active ingredients, extracts, etc.) on hyaluronic acid production (synthesis and release) by normal human epidermal keratinocytes.

    - And more other tests


    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!



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    Atopic Dermatitis Animal Models

    Price on request

    Atopic dermatitis, also known as atopic eczema, is a recurrent inflammatory skin disease that affects children in particular. It is due to an epidermal barrier dysfunction and to a hypersensitivity to environmental factors. Since the 2000s, the discovery of a preventive treatment for this severe dermatosis has been a special line of research in the pharmaceutical and dermocosmetics industry.

    Using its long-standing expertise in skin inflammation and in in vitro models, Bioalternatives initiated a research program on atopic dermatitis several years ago. This program has made it possible for us to develop and validate predictive in vitro pharmacological assays in order to limit attrition in clinical trials.


    The in vitro pharmacological assays listed below can be used for screening or evaluating potential modulators of atopic dermatitis (APIs, biosimilars, formulations, medical devices):
    - Immune response (Th2/Th22 cytokine release, Ig production, etc.)
    - Skin sensitization (basophil activation, histamine release, neuropeptide production, etc.)
    - Activation of inflammatory response of keratinocytes or reconstructed epidermis
    - Epidermal differentiation, skin integrity and lesions, barrier function and antimicrobial defenses
    - Analysis of specific markers by immunoassays or Rt-qPCR-qPCR


    Bioalternatives cells and tissues models:
    - Reconstructed Epidermis, Bioalternatives (RHE)
    - Normal Human Epidermal Keratinocytes (NHEK)
    - Mast cells
    - CD4+ T cells


    Readout:
    -RHE (basal or stimulated):
    Filaggrin
    -NHEK (stimulated):
    TSLP release
    Atopic dermatitis gene expression
    -Mast cells:
    Histamine release
    -CD4+ T cells (stimulated):
    IL-10 release
    IL-4 release
    or other Th2 cytokines
    TNF- alpha release


    Example of Bioalternatives assays:
    - Gene expression (cytokine stimulation)
    The purpose of our assays is to evaluate, via qPCR, the effect of compounds (molecules, active ingredients, extracts, etc.) on gene implicated in atopic dermatitis expression in normal human keratinocytes. A mix of pro-inflammatory cytokines is used to stimulate the cells in order to recreate the pathological tissue environment found in the chronic phase of atopic dermatitis.
    - Filaggrin expression (cytokine stimulation)
    The purpose of this test is to evaluate via immunohistfluorescence or immunohistochemistry the effect of compounds (molecules, active ingredients, extracts, etc.) on filaggrin expression in Reconstructed Human Epidermis (RHE)
    A mix of pro-inflammatory cytokines is used to stimulate the RHE in order to recreate the pathological tissue environment found in the chronic phase of atopic dermatitis. The markers are then immunolabeled and the intensity of fluorescence is quantified.
    - Mast cells degranulation, histamine release (compound 48/80 stimulation)
    The purpose of this test is to evaluate via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.) on histamine release in murine mast cells.
    - And more other tests


    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!


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    Pharmacology & Toxicology

    Price on request

    Our existing in vitro models can be used for regulatory safety and toxicity testing of your cosmetics and ingredients.


    Such tests can include:
    Cytotoxicity, including skin/eye irritation and corrosion
    Phototoxicity (chemically induced skin irritation)
    Mutagenicity (DNA damage)


    Bioalternatives cells and tissues models:
    2D models
    suspended cells
    3D models
    Skin explant


    Example of Bioalternatives assays:
    - Cytotoxicity assay
    This in vitro preliminary cytotoxicity assay evaluates the non-cytotoxic concentrations of a tested compound (molecule, active ingredient, extract) for a specific cell or tissue model (monolayer, RHE, skin explant) and associated culture conditions (culture medium, density, time and frequency of treatment, etc.). This assay is an essential precondition for carrying out biological assays (in vitro pharmacological assays).
    Once the tested compound has been given a concentration range, this assay allows cell or tissue integrity to be measured by microscopic observation and cell viability quantification to be evaluated by using MTT reduction (vital staining) or WST-8 (water-soluble tetrazolium dye).
    - Phototoxicity assay
    Bioalternatives offer to measure the phototoxicity of our test compound on 3T3 murine fibroblasts in absence and in presence of UVA irradiation. Cell viability, after test compound incubation, is assessed by using the neutral red uptake method.
    The phototoxic effect of the test compound will be assessed according to the Commission Directive 2000/33/EC Annex II “3T3/neutral red uptake” and the OECD guideline 432 from the 13 April 2004.
    - Cutaneous irritation assay, OCDE 42 bis
    Bioalternatives offer to determine the skin sensibilization effect of your compounds (molecules, active ingredients, extracts, etc.)
    In this model, skin irritation is measured on reconstructed epidermis with a measurement of cell viability by photometry (MTT).
    - And more other tests


    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!


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    Claim Substantiation

    Price on request

    Are you looking for solutions to support your claims? in vitro assays are highly effective for establishing proof-of-concept of your compounds (e.g. extracts, active ingredients, formulations). Bioalternatives, a premier in vitro dermatological testing service laboratory, can assist you at every step of the pre-clinical development of your products: screening of bioactive compounds, proof-of-concept studies, repositioning of active ingredients.


    Our knowledge of skin biology and its application to new target assessment, mechanistic understanding and claim substantiation are without question of the highest standards in the industry :

    - Epidermal regeneration

    - Skin barrier and hydration

    - Skin pigmentation

    - Skin aging

    - Skin protection

    - Skin vascularization

    - Skin inflammation

    - Sebaceous gland regulation

    - Adipocyte metabolism - Hair regeneration


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    Gene Expression Analysis

    Price on request

    As a researcher in the pharmaceutical industry or as a physician, you wish to carry out transcriptome analysis as part of your preclinical or clinical studies.
    Do you have biological samples (cells, tissues, human or animal biopsies), RNA or cDNA that are ready to be analyzed? Are you looking for a reactive high-quality contract research organization that can provide you with expertise and flexibility?
    Our laboratory and our special team dedicated to molecular biology methods would like to assist you with your studies.


    Routine assays:
    - Preparation and quality control on RNA (total RNA and microRNA), cDNA or DNA
    - Gene expression analysis by RT-qPCR (mRNA and microRNA) or PCRarrays (SYBR Green or Taqman)
    - Whole genome or miRNome expression analysis (Affymetrix platform)
    - Bioinformatic analysis (Biological processes and pathways analysis)


    Customized services:
    - Design and validation of PCR primers and probes
    - Design and preparation of qPCRarrays (384-well format)
    - in situ hybridization
    - Genome editing, construction of modified cell lines
    - Target gene silencing (SiRNA/ShRNA)
    - Target gene overexpression


    Our PCR arrays:
    PCR arrays are dedicated to gene expression analysis by quantitative PCR. They are sets of primers, on the same support (384-well microplate), selected according to a theme or a given biological process. Gene expression analysis by PCR arrays is considered as the most effective and cost-efficient method for studying a panel of targeted genes.
    This method can be used for applied research (sample or biological model characterization, identification of biomarkers, etc.) or for the discovery and selection of active ingredients. PCR arrays can also be used for the validation of results obtained after transcriptomic analysis.


    - Standard PCR arrays:
    Bioalternatives has a wide range of thematic ready-to-use PCR arrays (8 to128 genes) to perform gene expression studies:
    Tissue inflammation
    Oxidative stress
    Ageing
    Angiogenesis
    Psoriasis
    Atopic dermatitis
    Innate immunity and skin defense mechanisms
    Adipocyte differentiation and conversion
    Extracellular matrix synthesis and degradation (fibroblasts)
    Epidermal differentiation (keratinocytes)
    Cell junction and communication
    Pigmentation
    UV
    etc.


    - Our qPCR services include:
    Biological sample preparation and recovery
    Total RNA, mRNA and microRNA extraction and quality control using electrophoresis (Agilent)
    Reverse transcription and qPCR by SYBR Green or TaqMan probe
    Raw data
    Quality control and data normalization


    At Bioalternatives, we can also design and validate custom PCR arrays dedicated to the design of tailored arrangements that include the selection of your genes of interest (8 to 128 genes).


    Do not hesitate to contact us for any additional information.

    biomarker PCR analysis Gene Expression Cosmetics Dermatology Inflammation Oxidative Stress Pharmaceutical Show 8 more tags Show less

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    In vitro Skin Microbiome Models

    Price on request

    The human skin serves as the first natural physical barrier against external assaults while concomitantly providing a home for more than 1000 billion beneficial microorganisms. These microorganisms form the commensal resident skin flora also known as the cutaneous microbiota. The microbiota lives a symbiotic relationship with its host and interacts constantly with the adaptive immune system, thus creating a complex ecosystem which is unique to each individual but highly variable depending on several factors such as (temperature, ph, humidity, body area, life period, diet, etc.). This fragile balanced ecosystem helps to maintain healthy skin and protects it against the adhesion and development of pathogens. Its deterioration or imbalance can cause skin disorders (dry skin, oily skin, etc.) and amplify pathologies (psoriasis, atopic dermatitis, acne, etc.)
    Thanks to its expertise in cellular and tissue engineering, Bioalternatives has developed models that are suitable for analyzing the interactions between microbiota and skin and evaluate the efficacy of your products from in silico to clinical sample bioanalysis.


    Microbiota-friendly studies:
    - Bacterial growth and yeast growth: Photometry (OD), Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM)
    - Bacterial adhesion on RHE surface: Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM), Radioactivity, Scanning electron microscopy)
    - Bacterial & yeast quantification & identification:  Traditional microbiology on agar, Targeted qPCR, Non targeted metagenomic (MiSeq)


    Anti-microbial studies:
    - Biological target, active molecule: SELNERGY
    - Bacterial viability, Bacterial growth /Yeast growth, Bacterial identification: Photometry (OD) - MIC / MBC / TKC, Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM)
    - Bacterial adhesion on RHE surface, epidermal protein analysis (antimicrobial peptides), inflammation assay (cytokine release): Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM), Radioactivity, Scanning Electron Microscopy
    - Wound healing delay after bacterial infection: Wound diameter, tissue morphology (HES)
    - Bacterial quantification, bacterial identification: Traditional microbiology on agar, targeted qPCR, non targeted metagenomic (MiSeq).


    Bioalternatives offers a wide range of microorganisms tested by our laboratory and available for in vitro testing:
    GRAM (+)
    Staphylococcus aureus

    Staphylococcus epidermidis
    Cutibacterium acnes
    Corynebacterium xerosis
    Streptococcus pyogenes
    Mix of several strains (upon request)
    Other strains upon request
    GRAM (-)
    Pseudomonas aeruginosa
    Other strains upon request
    VIRUSES
    Herpes simplex virus type 1 (HSV-1)
    Human Rhinovirus 16 (HRV-16)
    YEAST
    Malassezia


    Example of Bioalternatives assays:
    - Bacterial enumeration
    The purpose of our test is to evaluate the effect of the compounds (molecules, active ingredients, extracts, etc.) on stimulating or inhibiting bacterial proliferation. This is done by bacterial enumeration using qPCR (DNA quantification) or by bacterial counting using colony forming unit (CFU). Products can be tested at different concentrations and on different time-point.
    - RHE, adhesion of a bacterial mix (basal)
    The purpose of our test is to evaluate via qPCR quantification the effect of compounds (molecules, active ingredients, extracts, etc.)  on the adhesion of a mix of 4 bacterial strains on reconstructed human epidermis.
    (This test can also be realized with an uncial strains)
    - NHDF, Cytokine/chemokine release
    The purpose of our test is to evaluate via ELISA quantification the effect of compounds (molecules, active ingredients, extracts, etc.)  on the inflammatory response induced by bacterial strain in human fibroblasts.
    (This test can also be realized in human sebocytes cell line)
    - And more other tests


    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!

    Microbiology Dermatology

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    Anti-Inflammatory Drug Screening in Human Cells

    Price on request

    Inflammation is a biological response of the immune system that can be triggered by a variety of factors, including pathogens, damaged cells and toxic compounds.


    Our laboratory can support anti-Inflammatory project in the following research areas:

    Cell and tissue damage:
    Acute inflammation or stress: innate immunity & epithelial barrier response against damage
    Defense mechanisms
    Chronic inflammation, stress or disease: tissue response, damage, ageing, etc.

    Regulation of immune and inflammatory responses:
    Recruitment, proliferation, differentiation, polarization and function of immune cells
    Cytokine, chemokine and growth factor release

    Tissue Regeneration:
    Tissue repair, wound healing
    Regeneration, proliferation and migration
    Differentiation process
    Function and recovery


    Bioalternatives cells and tissues models:

    Our immune blood cells
    Primary cells (human)
    Whole blood
    PBMC
    PMN
    CD4+ T lymphocytes
    CD19+ B lymphocytes B
    CD14+ monocytes, M1 and M2 macrophages
    Dendritic and langerhans cells
    Granulocytes
    Basophils
    Primary cells (murin)
    Splenocytes
    Thymocytes
    Mast cells
    Cell lines
    U937
    THP1
    HL-60
    Jurkat


    Readout:
    -Macrophage-like U-937 (basal or stimulated)
    IL-1 beta release
    IL-6 release
    IL-8 release
    TNF- alpha release
    Annexin V / PI
    PGE2 release
    TXB2 release
    -Thymocytes (basal or stimulated)
    Active caspase 3
    -THP-1 (basal or stimulated)
    Caspase-1 activity
    IL-1 beta release
    -T cells, (basal or stimulated)
    IFN- gamma release
    CD25 expression
    -PMN (basal or stimulated)
    IL-8 release
    IL-1 release beta
    CD11b/CD62L expression
    -PBMc (basal or stimulated)
    IFN- gamma release
    PI3K/AKT activation
    Cytokine release
    Inflammation genes
    -Mast cells (basal or stimulated)
    Histamine release 
    -HL-60
    mRNA expression
    Arachidonic acid metabolite release
    -CD4+ T cells
    Cytokine release
    -CD19+ B cells
    IL-6 release
    Ig production


    Example of Bioalternatives assays:
    - Blood, human basophil activation assay
    Immediate-type hypersensitivity is characterized by allergic reactions following the contact with an allergen. This allergic reaction can be mediated or not by immunoglobulin E (IgE) class of antibodies. The cross-linking of two IgE receptors (FceRI) by an allergen induces intracellular signaling pathways, thus resulting in the basophil activation. The ultimate step of basophil activation is the degranulation resulting in histamine release. Such histamine release is responsible for the appearance of allergy reactions.
    The degranulation step consists in the fusion of cytoplasmic granules with the basophil plasma membrane. CD63 is then exposed to the extracellular matrix and it is considered as a specific marker of activated basophils.
    The purpose of our test is to evaluate via flow cytometry the effect of compounds (molecules, active ingredients, extracts, etc.) on CD63 expression in CCR3+ basophils.
    - Mast cells degranulation, histamine release (compound 48/80 stimulation)
    Mast cells and basophils represent the most relevant source of histamine in the immune system. Histamine is stored in cytoplasmic granules. Histamine release is regulated by several activating and inhibitory receptors. In conventional allergy, presence of allergen specific IgE activate mast cell degranulation and histamine release.
    The purpose of our test is to evaluate via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.) on histamine release in rat stimulated mast cells.
    - Peripheral Blood Mononuclear Cells stimulated monocytes, cytokine release (LPS or anti-CD3/anti-CD28 stimulation)
    The purpose of our test is to evaluate via flow cytometry (multiplex) the effect of compounds (molecules, active ingredients, extracts, etc.)  on cytokine in stimulated Peripheral Blood Mononuclear Cells (PBMC).
    - And more other tests


    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!


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    In vitro Acne Models

    Price on request

    Acne is a chronic inflammatory pathology of the pilosebaceous follicle under hormonal dependence that includes:
    -abnormal increase of sebaceous lipid production (sebum)
    -formation of microcomedones due to an abnormal epidermal differentiation, a likely consequence of abnormal androgen (testosterone) metabolism
    -accumulation of peroxidized lipids on the surface (blackheads) or inside the comedo (whitehead)
    formation of infectious and non-infectious inflammatory lesions and a skin wound healing process


    Bioalternatives provides you with a range of innovative in vitro pharmacological models and assays, more particularly dedicated to the evaluation of the pharmacological efficacy (screening, profiling, proof-of-concept) of your products (APIs, biosimilars, fomulations, medical devices):
    -Sebaceous gland regulation and hyperseborrhea
    -Androgen metabolism
    -Abnormal epidermal differentiation
    -Lipid peroxidation
    -Inflammatory, lesional or microbial response
    -Infection, immune system and skin defenses


    Bioalternatives cells and tissues models:
    -Human sebocytes: SEBO662 and SEBO662AR cell lines
    -Normal human epidermal keratinocytes (NHEK)
    -Normal human dermal fibroblasts (NHDF)
    -Human follicle dermal papilla cells (HFDPC)
    -Reconstructed human epidermis (RHE)


    Readout:
    -SEBO662 (basal)
    testosterone metabolism
    5- alpha reductase activity
    -SEBO662AR (basal or stimulated)
    Lipogenesis
    -NHEK (Basal)
    Antimicrobial peptide


    Example of Bioalternatives assays:
    - Keratinocytes antimicrobial peptide release
    Elafin and beta-defensin 2 are potent antimicrobial peptides which are notably expressed in normal human keratinocytes. A high level of expression of these two peptides in keratinocytes is frequently associated with inflammatory skin lesions.
    The purpose of our test is to evaluate via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.)  on peptide release (beta-defansin, elafin) in human keratinocytes.
    - Sebocytes, testosterone metabolism & 5-a reductase activity
    Acne is a multiparametric skin disorder in which sebum hypersecretion induced by circulating testosterone is involved. Testosterone is converted, by 5α-reductase, into dihydrotestosterone (DHT), one of its biologically active metabolites. Moreover, an excess of sebum (hyperseborrhea) also favors the development of bacteria (i.e. Propionibacterium acnes) which in turn induces inflammation. The inhibition of 5α-reductase enzymatic activity and/or inflammation is known to reduce acne in human skin.
    The purpose of our assays is to evaluate, via [14C]-testosterone incorporation, the effect of compounds (molecules, active ingredients, extracts, etc.) on 5-alpha reductase activity in human sebocytes 
    - Sebocyte, lipogenesis
    Acne is an inflammatory pathology linked to an abnormal production of sebum by the sebocytes. Sebum is produced because of a complex process of lipid synthesis, lipogenesis and then released by holocrine secretion. The production is under hormonal control such as testosterone known to stimulate the production of sebum. The stimulation by a complete testosterone results in an even stronger increase of the lipid synthesis and storage.
    The purpose of our assays is to evaluate, via fluorescence, the effect of compounds (molecules, active ingredients, extracts, etc.) on lipid production in a model of human sebocytes cell line
    - And more other tests


    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!

    Fluorescent labeling PCR Immunofluorescence

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    In vitro Psoriasis Models

    Price on request
    Psoriasis is a non-contagious inflammatory skin disease linked to a genetic predisposition and which develops under the influence of environmental parameters. While no treatment can completely cure this autoimmune disease, psoriasis is the subject of intensive research and especially so since the identification of subpopulations of Th17 and Th22 lymphocytes.

    Bioalternatives has carried out an extensive research program on psoriasis  and can offer a full range of innovative models and in vitro pharmacology assays specifically dedicated to the evaluation of pharmacological efficacy (screening, profiling, proof-of-concept) of your products (APIs, biosimilars, formulations, medical devices):
    -Immune response (Th17/Th22 cytokine release)
    -Induction in keratinocytes (or reconstructed epidermis) of a psoriasis-like profile by cytokines
    -Production of chemokines, antimicrobial peptides (AMPs) and expression of epidermal differentiation markers
    -Specific marker analysis by immunoassays or RT-qPCR

    Bioalternatives cells and tissues models:
    Reconstructed Epidermis, Bioalternatives (RHE)
    Normal Human Epidermal Keratinocytes (NHEK)
    CD4+ T cells

    Readout:
    -RHE (basal or stimulated):
    S100A7
    Beta-defensin 2/4
    RNAse7
    -NHEK (stimulated):
    STAT3 activation
    NF kappaB translocation
    -CD4+ T cells (stimulated):
    IL-17 release

    Example of Bioalternatives assays:
    - Keratinocyte, gene expression (cytokine stimulation)
    The purpose of our assays is to evaluate, via qPCR, the effect of compounds (molecules, active ingredients, extracts, etc.) on gene implicated in psoriasis expression in normal human keratinocytes. A mix of pro-inflammatory cytokines is used to stimulate the cells in order to recreate the pathological tissue environment found in the chronic phase of psoriasis.
    (This test can also be realized in Reconstructed Human Epidermis (RHE))
    - Kératinocytes antimicrobial peptide release (cytokine stimulation)
    The purpose of our assays is to evaluate, via ELISA, the effect of compounds (molecules, active ingredients, extracts, etc.) on peptides implicated in psoriasis expression (beta defebsin 2, S100A7, elafin) in normal human keratinocytes. A mix of pro-inflammatory cytokines is used to stimulate the cells in order to recreate the pathological tissue environment found in the chronic phase of psoriasis
    - Reconstructed Human Epidermis), skin innate immunity markers expression (cytokine stimulation)
    The purpose of our assays is to evaluate, via immunohistochemistry or immunohistofluorescence, the effect of compounds (molecules, active ingredients, extracts, etc.) on skin innate markers implicated in psoriasis expression (beta defebsin 2/4, S100A7, RNAse 7) in normal human keratinocytes. A mix of pro-inflammatory cytokines is used to stimulate the cells in order to recreate the pathological tissue environment found in the chronic phase of psoriasis
    - And more other tests

    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!

    Gene expression profiling PCR ELISA Flow cytometry western blot histology Immunofluorescence immunohistochemistry Human Show 9 more tags Show less

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    In vitro Wound Healing Assay

    Price on request
    Wound healing is a complex and dynamic process of restoring skin cellular structures and tissue layers that involves multiple components: differentiated cells, stem cells, hair follicles, extracellular matrix (ECM) proteins, cytokines networks, microRNAs , blood vessels, nerves  and mechanical forces.
    There is 4 interrelated and overlapping phases:
    -haemostasis,
    -inflammation,
    -granulation/proliferation
    -maturation/remodelling

    Thanks to its expertise Bioalternative can evaluate the pro-healing effect of active compounds, formulations or medical devices by measuring:
    -inflammatory response, cleaning of skin wounds and innate immunity
    -reepithelialization
    -neovascularization and neuritogenesis
    -extracellular matrix synthesis, fibrosis and myofibroblast interaction
    -dermal remodeling and epidermal differentiation
    -pigmentation

    Bioalternatives cells and tissues models:
    -Cell lines
    -Peripheral blood immune cells (PBMCs and purifed blood cell populations: subpopulations of T lymphocytes, monocytes, polymorphonuclear cells, etc.)
    -Macrophages and monocyte-derived dendritic cells
    -Normal human epidermal keratinocytes (NHEK)
    -Normal human dermal fibroblasts (NHDF)
    -Myofibroblasts
    -Mesenchymal stem cells
    -Microvascular endothelial cells
    -Normal human epidermal melanocytes (NHEM)
    -Sensory neurons
    -Reconstructed human epidermis (RHE)
    -Dermal equivalents (DE)
    -Skin explants (ex vivo)

    Example of Bioalternatives assays:
    - Fibroblast, IL-8 release (IL-1a stimulation)
    The purpose of our assays is to evaluate, via ELISA, the effect of compounds (molecules, active ingredients, extracts, etc.) on IL-8 release in normal human keratinocytes stimulated with IL-1-alpha.
    - Keratinocytes, migration/proliferation
    Epidermal regeneration is a complex and dynamic process responsible for restoring the cellular and structural components of the skin, using the mechanisms of keratinocyte proliferation and migration as well as extracellular matrix remodeling.
    The purpose of our test is to evaluate via fluorescence/imaging the capacity of a compound to stimulate the cell migration process, using the Oris™ Cell Migration assay in normal human keratinocytes.
    - Keratinocyte, differentiation markers
    Bioalternatives has developed an assay to determine keratinocyte differentiation on Normal Human Epidermal Keratinocytes cultivated in low calcium medium.
    The purpose of our in vitro assay is to evaluate the compound capacity to modulate the expression of early (K1, K10, involucrin….) or later (Loricrin, Transglutaminase, Filagrin…) differentiation markers on normal human keratinocytes, by using Rt-qPCR.   
    - And more other tests

    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!

    Immunofluorescence Flow cytometry ELISA PCR

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    Dermal Pharmacology & Efficacy Studies

    Price on request

    Bioalternatives developed and validated in vitro cell and tissue models (e.g. normal or aged dermal fibroblasts, full thickness skin) and assays for investigating the anti-aging effect of cosmetics compounds, such as:
    -cell migration
    -proliferation or differentiation assays
    -extracellular matrix synthesis or degradation assays (e.g. collagen or tropoelastin synthesis/ expression, MMP activities)
    -gene expression assays
    -reinforcement of dermis cohesion and firmness
    -production of extracellular matrix components and improvement of cell-matrix interactions (collagen I, collagen III), elastin, fibronectin, proteoglycans, glycoaminoglycans  and hyaluronic acid proteases (MMPs) etc.).
    -improvement of dermo-epidermal junction quality and cell adhesion (integrins, laminin V, collagen IV, collagen VII, desmogleins, etc.).


    Bioalternatives cells and tissues models:
    -normal human dermal fibroblasts (NHDF)
    -dermal equivalent (DE)
    -normal human epidermal keratinocytes (NHEK)
    -reconstructed human epidermis (RHE)
    -full thickness reconstructed skin (FTSK)
    -skin explants (ex vivo)

    Example of Bioalternatives assays:
    - Dermal equivalent, dermis strength and cohesion
    The purpose of our assay is to evaluate via imaging the effect of compounds (molecules, active ingredients, extracts, etc.) on lattice contraction in dermis equivalent.
    - Fibroblast, procollagen I synthesis/release
    In the dermis, fibroblasts continuously secrete the precursors of all the components of the extracellular matrix, thus maintaining the skin structural integrity. The extracellular matrix is a complex structure formed of a very well-organized network of collagen fibers, elastic fibers and reticular fibers. Fibril collagens are the most abundant proteins present in human skin, creating more than 90% of its dry weight. Type I collagen represent 60 to 80% of collagens of the dermis and the hypodermis. Type I, II and V fibril collagens self-assemble in thicker fibers forming a tridimensional network in the entire thickness of the dermis. They give to the skin its resilience strength and are essential to the integrity of the tissue. Collagen network is organized and maintained under dynamic mechanical tension provided by fibroblasts responsible of its production.
    The purpose of our assay is to evaluate via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.) on Procollagen I synthesis / release in normal human fibroblasts.
    - Fibroblast, extracellular matrix gene expression
     The purpose of our assay is to evaluate, via RT-qPCR  technology, the capacity of compounds to modulate the gene expression profile of normal fibroblasts.  Extracted mRNA is analyzed on a dedicated PCR array (mQPA H-NHDF-16 or 64) designed by Bioalternatives and containing 16 or 64 target genes (including housekeeping genes) selected for their importance in the fibroblast physiology and the synthesis or degradation of Extracellular matrix.
    - And more other tests

    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!

    qPCR primary cell culture ELISA Immunofluorescence Human Aging Immunology Show 7 more tags Show less

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    In vitro Hair Growth Model

    Price on request
    Hair is a unique feature of each individual and, more generally, of the human species. It is estimated that adults have about 5 million hair follicles, with 1 million hair follicles found on the head, and with only 120,000 to 150,000 hair follicles covering the scalp. Depending on the region of the scalp, their density varies by 200 to 300 follicles /cm2. A hundred hair follicles are naturally shed each day. The speed at which hair grows is about 0.3 mm per day or 12 cm per year. This speed is affected by numerous factors, such as follicle location, gender, age and ethnicity of the individual as well as environmental factors.

    Bioalternatives developed and validated an ex-vivo model of human hair follicle suitable for investigating the effect of compounds on:
    -the stimulation/ inhibition of hair growth
    -the survival rate increase in cell cultures (anti apoptosis, anti-hair loss focus)
    -the hair pigmentation.
    -the increase in cell viability in culture (anti-apoptosis, anti-hair loss)
    -the increase of the hair shaft diameter
    -5-alpha reductase activity (androgen metabolism)
    -specific marker expression (Ki67, TGK, fibronectin, β-catenin, cytokines etc.)

    These parameters can be analyzed by morphometric techniques (hair elongation and viability measurement) but also through complex molecular approaches:
    - gene expression (dedicated PCRarrays or full genome profil)
    - immunohistochemistry or histology as special staining e.g. Fontana Masson for pigmentation.

    Bioalternatives cells and tissues models:
    - human follicle dermal papilla fibroblasts (HFDPC)
    - human hair follicle (ex vivo)

    Readout:
    - Hair follicle:
    Protein expression (Ki67, K15, K19, TGK, collagen 1, fibronectin, etc.).
    Gene expression
    - HFDPC:
    Testosterone metabolism
    5-alpha reductase activity

    Example of Bioalternatives assays:
    - Hair follicle growth & degeneration
    Our ex vivo hair follicle model is directly derived from the widely recognized standard conditions described by Philpott et al. It represents a “natural” co-culture model of human cutaneous cells (keratinocytes, fibroblasts, melanocytes and stem cells).
    The purpose of our assay is to evaluate the stimulating or inhibiting effects of compounds (molecules, active ingredients, extracts, etc.) on hair growth or hair survival (anti-apoptosis, anti-hair loss) by using morphometric techniques (images and measurements).
    - Human Follicle Dermal Papilla Cells, testosterone metabolism & 5-a reductase activity
    The conversion of testosterone to 5-dihydrotestosterone (DHT), the active metabolite of testosterone, by the 5α-reductase enzyme is one of the causes of sebaceous gland hypersecretion and of premature hair loss.
    The purpose of our assay is to evaluate the effects of compounds (molecules, active ingredients, extracts, etc.) on the 5-α reductase activity of human hair follicle dermal papilla fibroblasts (HFDPC). This assay is based on the incorporation of [14C]-testosterone and on the extraction and separation/radiodetection of the formed metabolites. In particular, it allows the production of the active metabolite of testosterone (dihydrotestosterone) to be measured.
    - Hair follicle, protein expression
    Hair follicles are located under the skin and are divided into two main parts: one is of epithelial origin and is composed of the matrix (reserve of highly replicative matrix cells essential for hair follicle growth), the shaft, the internal root sheath and the external epithelial root sheath made of keratinocyte filaments; the other part is of mesenchymal origin and comprises the connective tissue sheath made of collagen and proteoglycans and the dermal papilla (reserve of fibroblasts) which is separated from the epithelial compartment by the basement membrane.
    The purpose of our assay is to evaluate the stimulating or inhibiting effects of compounds (molecules, active ingredients, extracts, etc.) on hair follicles in culture with regard to the expression and/or localization of relevant proteins (e.g., Ki67, K15, K19, TGK, collagen I, fibronectin, etc.) by immunohistochemistry or immunofluorescence.
    - And more other tests

    Other additional information (graphics, illustrations) are also provided on our website
    Contact us!

    Alopecia Hypertrichosis Hirsutism microscopy qpc immunohistochemistry histology Immunofluorescence ELISA Human Show 10 more tags Show less

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    Angiogenesis & Endothelial Cell Assays

    Price on request

    Dermal arterioles and venules are composed of endothelial cells, connective tissues (collagen, elastin) and of smooth muscle cells which allow vessel vasoconstriction and vasodilation. Dermal capillaries are composed of a single layer of endothelial cells and of pericytes surrounded by a basal membrane.
    Angiogenesis involves the formation of capillaries from preexisting microvessels and therefore contributes to vascular remodeling and maturation. It plays a pivotal function in a variety of normal and pathological conditions such as embryonic development, the menstrual cycle, hair cycle, wound healing, arthritis, psoriasis, proliferative diabetic retinopathy, atherosclerosis, post ischemic vascularization of the myocardium and tumor growth and metastasis.
    Microcirculation can be altered by external factors and also skin ageing, with a progressive decrease of new blood vessel formation.


    Bioalternatives can evaluate the effect of active cosmetic compounds on skin microvascularization by measuring:
    -Viability, proliferation and migration of endothelial cells
    -Cell differentiation and pseudotube (angiogenesis, neovascularization) formation
    -Protection of endothelial cells against stress (inflammation, infection, etc.) 
    -Veinotonic activity of smooth muscle cells
    -Angiogenic factor release (e.g.VEGF)


    Bioalternatives cells and tissues models:
    -Human umbilical vein endothelial cells (HUVEC)
    -Human dermal microvascular endothelial cells (HMVEC-d)
    -HMVEC-d and NHDF coculture (ENDOF)
    -Human aortic smooth muscle cells (AoSMC)
    -Other skin cells (NHEK, NHDF, etc.)

    Readout
    -NHDF:
    VEGF release
    -HUVEC:
    ICAM-1 expression


    Example of Bioalternatives assays:
    - Differentiation/pseudotube formation (VEGF stimulation, anti-angiogenic agents)
    Angiogenesis involves the formation of capillaries from preexisting microvessels and therefore contributes to vascular remodeling and maturation. It plays a pivotal function in a variety of normal and pathological conditions such as embryonic development, the menstrual cycle, hair cycle, wound healing, arthritis, psoriasis, proliferative diabetic retinopathy, atherosclerosis, post ischemic vascularization of the myocardium and tumor growth and metastasis. The initiation of the physiopathological angiogenic response, known as the ‘angiogenic switch’, depends on the dynamic balance between exogenous or endogenous stimuli (pro-angiogenic factors) and inhibitors (anti-angiogenic factors) acting in the immediate environment of endothelial cells.
    The in vitro assay proposed below consists in a co-culture model of normal human endothelial cells with normal human dermal fibroblasts. After several days of incubation, the treatment of the co-culture with the pro-angiogenic factor VEGF results in the organization of endothelial cells in pseudotubes. Therefore, under VEGF-stimulated condition, this model is suitable to evaluate test compounds capable of inhibiting the formation of this pseudotube network. The pseudotube formation in this model is analyzed by image analysis further to the specific immunolabelling of endothelial cells only using an anti-VWF antibody.
    Remark : Under basal conditions (in absence of VEGF), our co-culture model can also be used to screen compounds capable of promoting the development of the pseudotube.
    - Veinotonic activity
    Vascular smooth muscle cells play an essential role on the microvascular system and most vasoconstrictors/ veinotonic exert their effects by acting directly on the smooth muscle cells of the vascular wall. The contraction of smooth muscular cells, in most cases linked to an increase of cytoplasmic calcium, causes the vasoconstriction of nearby blood vessels.
    The purpose of this assay is to evaluate, via time-lapse fluorescence microscopy, the capacity of the compounds to stimulate the contraction of primary smoth vascular muscle cell by measuring their capacity to induce the mobilization of intra-cellular calcium.
    - VEGF release
    The purpose of our assay is to evaluate, via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.) on VEGF release in normal human fibroblast.

    - And more other tests


    Other additional information (graphics, illustrations) are also provided site on our website
    Contact us!



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    Cell and Tissue Culture

    Price on request

    Cell and tissue engineering, development of new comple in vitro mammalian models:

    • Tissue microdissection
    • Primary cell isolation
    • Cell line construction
    • Co-culture model system
    • Organotypic explant culture
    • Three dimensional reconstruction
    • Phenotypic and functional characterization


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    Cell-Based Assays

    Price on request

    Cellular and molecular pharmacology assays for hit-to-lead selection of active pharmaceutical ingredients and claim substantiation of healthcare products:

    • cell-based assays
    • Screening
    • Profiling
    • Proof-of-concept
    • Mechanism of action
    • Benchmarking
    • Safety
    • Testing of formulations prior clinical trial


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    Assay Development

    Price on request

    Services for the development, customization and optimization of innovative research methods and protocols :

    • Literature review
    • Feasability study
    • Protocol design
    • Method validation
    • Multiplexing, miniaturization and optimization

    ELISA Flow cytometry western blot Immunoassays Immunofluorescence immunohistochemistry histology qPCR Fluorescent labeling radiochemistry Biochemistry Show 11 more tags Show less

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    Bioanalysis

    Price on request

    Biological sample collection, processing, storage and information management:

    • Study design
    • Sample Collection
    • Method develoment and validation (tissue microarrays, cDNArrays, PCRarrays, immunoassays, biochemical assays)
    • Analysis service
    • Biobanking (RNA, cDNA, cells, frozen tissues, embedded tissues)
    • Data management


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    Cell Migration Assays

    Price on request

    Cell migration is commonly assessed using the scratch assay which measures the movement of cells into an artificial wound made on a confluent cell monolayer to create a cell-free area. As an alternative to the scratch assay which has high variability, the Oris™ Cell Migration Assay allows the formation of homogeneously sized cell-free areas into which migration can occur without releasing factors from dead cells or damaging the underlying extracellular matrix.


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    Cell Proliferation Assays

    Price on request

    - BrdU cell proliferation assay

    - 3H thymine incorporation assay 

    Biology

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    In vitro Reporter Gene Assays

    Price on request

    Cell-Based Gene Expression Assays using qPCR (SYBR Green or TaqMan) or microrrays (Affymetrix);

    LightCycler Affymetrix GeneAtlas system

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    Human Skin Acute Inflammation Assays

    Price on request
    Request a quote for more information about this service.

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    Antiviral Testing

    Price on request
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    Cytokine Analysis

    Price on request
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    ELISA Flow cytometry

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    Drug Mechanism of Action Studies

    Mechanism of Action Studies
    Price on request
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    Cells and Tissues

    Price on request
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    Medicinal Chemistry

    Price on request
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    Cell Invasion & Migration Assays

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    Biology

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    Functional Human Tissue Assays

    Price on request
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    Drug Discovery

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    Project Management

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    In vitro Disease Models

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    Product Development, Testing, and Packaging

    Price on request
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    Drug Discovery & Development

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    Cell Viability & Proliferation Assays

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    Safety Pharmacology

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    Immunoassays

    Price on request
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    Cell-Based Compound Screening

    Price on request
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    Compound Profiling

    Price on request
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    In vitro Immunogenicity Assays

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    Omics

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    GT

    Guillaume Tenca

    Sales Manager
    MV

    Maxance Vandevyvere

    Business Developer, Legal & IP Manager

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