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Bioalternatives

12 Orders Completed
Gençay, FR

About Bioalternatives

Founded: 1996 Type: Partnership Size: 11-50 employees

Bioalternatives is a contract research organization (CRO) specializing in cellular and molecular pharmacology. It was founded in 1996 by Dr. François-Xavier BERNARD and Dr. Alain DEGUERCY.


Our laboratory is committed to...

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Bioalternatives is a contract research organization (CRO) specializing in cellular and molecular pharmacology. It was founded in 1996 by Dr. François-Xavier BERNARD and Dr. Alain DEGUERCY.


Our laboratory is committed to developing effective in vitro alternative options to animal experimentation methods, by offering various in silico, in vitro and ex vivo complementary services that are suitable for drug discovery (screening, profiling, safety) and can also provide you with assays for claim substantiation (proof of concept, repositioning, benchmarking).


Beyond this historical product evaluation and optimization activity, Bioalternative assists the health industry in its new strategic choices by offering competitive custom-oriented and collaborative innovation services.


Our main fields of study are healthy and/or pathological skin (e.g. psoriasis, atopic dermatitis, wound healing, fibrosis, etc.), immune-inflammation and more generally pharmacology (signaling, stress, ageing).

Company Facts

  • Founded in 1996
  • Employees: 47 (32 scientists)
  • Corporate Headquarters: Gençay, Vienne France
  • Subsidiarie: Bioalternatives Inc.New York, NY, USA
  • 1000 square meter laboratory
  • 500+ in vitro developed assays - 300+ studies/year - Cumulative R&D spent > 15 % of turnover
  • Research tax credit (Crédit Impôt Recherche) accreditation (France)

Recent Publications

  • REGNACQ M., VOISIN P., SERE YY., WAN B., SOEROSO VM., BERNARD M., CAMOUGRAND N., BERNARD FX., BARRAULT C., BERGES T. (2016) Increased fatty acid synthesis inhibits nitrogen starvation-induced autophagy in lipid droplet-deficient yeast. Biochemical and Biophysical Research Communications, 1-7.
  • RABEONY H., POHIN M., VASSEUR P., PETIT-PARIS I., JÉGOU JF., FAVOT L., FROUIN E., BOUTET MA., BLANCHARD F., TOGBE D., RYFFEL B., BERNARD FX., LECRON JC., MOREL F. (2015) IMQ-induced skin inflammation in mice is dependent on IL-1R1 and MyD88 signaling but independent of the NLRP3 inflammasome. European Journal of Immunology, 45(7):2847-2857.
  • BARRAULT C., GARNIER J., PEDRETTI N., CORDIER-DIRIKOC S., RATINEAU E., DEGUERCY A., BERNARD FX. (2015) Androgens induce sebaceous differentiation in sebocyte cells expressing a stable functional androgen receptor. The Journal of Steroid Biochemistry and Molecular Biology, 152:34-44.
  • AKIL H., ABBACI A., LALLOUÉ F., BESSETTE B., COSTES LM., DOMBALLE L., CHARREAU S., GUILLOTEAU K., KARAYAN-TAPON L., BERNARD FX., MOREL F., JAUBERTEAU MO., LECRON JC. (2015) IL22/IL-22R Pathway Induces Cell Survival in Human Glioblastoma Cells.
    PLoS ONE, 10(3)
  • MCHEIK JN., BARRAULT C., LEVARD G., MOREL F., BERNARD FX., LECRON JC. (2014)
    Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective.
    Plastic and Reconstructive Surgery Global Open, 2(9)
  • MCHEIK JN., BARRAULT C., PEDRETTI N., GARNIER J., JUCHAUX F., LEVARD G., MOREL F., BERNARD FX. and LECRON JC. (2014)
    Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children.
    Burns, 41(2):352-8
  • RABEONY H., PETIT-PARIS I., GARNIER J., BARRAULT C., PEDRETTI N., GUILLOTEAU K., JEGOU JF., GUILLET G., HUGUIER V., LECRON JC., BERNARD FX. and MOREL F. (2014) Inhibition of Keratinocyte Differentiation by the Synergistic Effect of IL-17A, IL-22, IL-1α, TNFα and Oncostatin M. PLOS ONE, 9(7)
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Our Services (43)


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Cosmetic and Personal Care Product Testing

Price on request

In vitro and ex vivo efficacy studies of cosmetic products: oily skin, dry skin, hydration, skin ageing, pigmentation, hair growth, etc.


You are looking for validated or innovative efficacy assays to support your cosmetic claims? You wish to characterize your active ingredients, demonstrate the... Show more »

In vitro and ex vivo efficacy studies of cosmetic products: oily skin, dry skin, hydration, skin ageing, pigmentation, hair growth, etc.


You are looking for validated or innovative efficacy assays to support your cosmetic claims? You wish to characterize your active ingredients, demonstrate the efficacy of your cosmetic formulations? Or would you like to test the safety of your products at an early stage? Our services will help you meet your objectives and support your projects.


With our strong expertise in numerous in vitro and ex vivo models and in skin physiology, our aim is to bring value to your cosmetic products:
- Screening of bioactive ingredients
- Mechanism of action and proof-of-concept
- in vitro safety assays (non-GLP)
- Repositioning or benchmarking studies
- in vitro or ex vivo evaluation of cosmetic formulations before clinical trials.


As your partner in innovation, we provide you with new concepts to substantiate your claims
Let’s talk about it!

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Biology Cosmetics

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Product Testing Services

Price on request

At Bioalternatives, we are committed to developing effective in vitro alternative options to animal experimentation methods, by offering a full range of solutions for the development of active ingredients and cosmetic formulations.
We offer customized technical solutions to guide your product research... Show more »

At Bioalternatives, we are committed to developing effective in vitro alternative options to animal experimentation methods, by offering a full range of solutions for the development of active ingredients and cosmetic formulations.
We offer customized technical solutions to guide your product research and support the claims of your cosmetic products. Our selection of in vitro testing solutions can be used to characterize your cosmetic products’ active ingredients, demonstrate the efficacy of your formulations, and test the safety of your cosmetic products at an early stage (for R&D purposes only).
With extensive experience in cosmetic product testing and state-of-the-art facilities, we are pleased to offer you dedicated project management support and consulting for your R&D process.


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Pharmacology

Price on request

Bioalternatives provides services to support your research needs in biology and in in vitro pharmacology. For more than 20 years, we have been assisting scientists from the pharmaceutical and biotechnological industry on preclinical issues (exploratory research, in vitro modelling, discovery of therapeutic... Show more »

Bioalternatives provides services to support your research needs in biology and in in vitro pharmacology. For more than 20 years, we have been assisting scientists from the pharmaceutical and biotechnological industry on preclinical issues (exploratory research, in vitro modelling, discovery of therapeutic molecules).
Because each of our clients is unique and each study is based on different prerequisites and objectives, we focus on the customized technical solution that can best meet your requirements. We pay particular attention to the feasibility study and to the personalized management of your project, especially through regular and open communication between your study manager and our specialists.
in vitro pharmacological studies are our core business: by managing a plurality of projects, our scientists have acquired an expertise and know-how which can be applied to a wide range of products.


Pharmaceutical products and active ingredients:
- Natural extracts (plant, marine, bacterial)
- Synthetic molecules, peptides, proteins, glycoproteins, lipids
- Biotherapies and biosimilars (antibodies, growth factors)
- Formulations (creams, gels, lotions)
- Medical devices


in vitro pharmacological studies:
- 2nd or 3rd intention screening
- Activity profiling
- Proof-of-concept, study of mechanism of action
- Confirmatory assay
- Biosimilarity

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Pharmaceutical

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Pharmacology & Toxicology

Price on request

Our existing in vitro models can be used for regulatory safety and toxicity testing of your cosmetics and ingredients.


Such tests can include:
Cytotoxicity, including skin/eye irritation and corrosion
Phototoxicity (chemically induced skin irritation)
Mutagenicity (DNA... Show more »

Our existing in vitro models can be used for regulatory safety and toxicity testing of your cosmetics and ingredients.


Such tests can include:
Cytotoxicity, including skin/eye irritation and corrosion
Phototoxicity (chemically induced skin irritation)
Mutagenicity (DNA damage)


Bioalternatives cells and tissues models:
2D models
suspended cells
3D models
Skin explant


Example of Bioalternatives assays:
- Cytotoxicity assay
This in vitro preliminary cytotoxicity assay evaluates the non-cytotoxic concentrations of a tested compound (molecule, active ingredient, extract) for a specific cell or tissue model (monolayer, RHE, skin explant) and associated culture conditions (culture medium, density, time and frequency of treatment, etc.). This assay is an essential precondition for carrying out biological assays (in vitro pharmacological assays).
Once the tested compound has been given a concentration range, this assay allows cell or tissue integrity to be measured by microscopic observation and cell viability quantification to be evaluated by using MTT reduction (vital staining) or WST-8 (water-soluble tetrazolium dye).
- Phototoxicity assay
Bioalternatives offer to measure the phototoxicity of our test compound on 3T3 murine fibroblasts in absence and in presence of UVA irradiation. Cell viability, after test compound incubation, is assessed by using the neutral red uptake method.
The phototoxic effect of the test compound will be assessed according to the Commission Directive 2000/33/EC Annex II “3T3/neutral red uptake” and the OECD guideline 432 from the 13 April 2004.
- Cutaneous irritation assay, OCDE 42 bis
Bioalternatives offer to determine the skin sensibilization effect of your compounds (molecules, active ingredients, extracts, etc.)
In this model, skin irritation is measured on reconstructed epidermis with a measurement of cell viability by photometry (MTT).
- And more other tests


Other additional information (graphics, illustrations) are also provided on our site on our website
Contact us!

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Gene Expression Analysis

Price on request

As a researcher in the pharmaceutical industry or as a physician, you wish to carry out transcriptome analysis as part of your preclinical or clinical studies.
Do you have biological samples (cells, tissues, human or animal biopsies), RNA or cDNA that are ready to be analyzed? Are you looking for a... Show more »

As a researcher in the pharmaceutical industry or as a physician, you wish to carry out transcriptome analysis as part of your preclinical or clinical studies.
Do you have biological samples (cells, tissues, human or animal biopsies), RNA or cDNA that are ready to be analyzed? Are you looking for a reactive high-quality contract research organization that can provide you with expertise and flexibility?
Our laboratory and our special team dedicated to molecular biology methods would like to assist you with your studies.


Routine assays:
- Preparation and quality control on RNA (total RNA and microRNA), cDNA or DNA
- Gene expression analysis by RT-qPCR (mRNA and microRNA) or PCRarrays (SYBR Green or Taqman)
- Whole genome or miRNome expression analysis (Affymetrix platform)
- Bioinformatic analysis (Biological processes and pathways analysis)


Customized services:
- Design and validation of PCR primers and probes
- Design and preparation of qPCRarrays (384-well format)
- in situ hybridization
- Genome editing, construction of modified cell lines
- Target gene silencing (SiRNA/ShRNA)
- Target gene overexpression


Our PCR arrays:
PCR arrays are dedicated to gene expression analysis by quantitative PCR. They are sets of primers, on the same support (384-well microplate), selected according to a theme or a given biological process. Gene expression analysis by PCR arrays is considered as the most effective and cost-efficient method for studying a panel of targeted genes.
This method can be used for applied research (sample or biological model characterization, identification of biomarkers, etc.) or for the discovery and selection of active ingredients. PCR arrays can also be used for the validation of results obtained after transcriptomic analysis.


- Standard PCR arrays:
Bioalternatives has a wide range of thematic ready-to-use PCR arrays (8 to128 genes) to perform gene expression studies:
Tissue inflammation
Oxidative stress
Ageing
Angiogenesis
Psoriasis
Atopic dermatitis
Innate immunity and skin defense mechanisms
Adipocyte differentiation and conversion
Extracellular matrix synthesis and degradation (fibroblasts)
Epidermal differentiation (keratinocytes)
Cell junction and communication
Pigmentation
UV
etc.


- Our qPCR services include:
Biological sample preparation and recovery
Total RNA, mRNA and microRNA extraction and quality control using electrophoresis (Agilent)
Reverse transcription and qPCR by SYBR Green or TaqMan probe
Raw data
Quality control and data normalization


At Bioalternatives, we can also design and validate custom PCR arrays dedicated to the design of tailored arrangements that include the selection of your genes of interest (8 to 128 genes).


Do not hesitate to contact us for any additional information.

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biomarker PCR analysis Gene Expression Cosmetics Dermatology Inflammation Oxidative Stress Pharmaceutical Show 8 more tags Show less

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In vitro Skin Microbiome Models

Price on request

The human skin serves as the first natural physical barrier against external assaults while concomitantly providing a home for more than 1000 billion beneficial microorganisms. These microorganisms form the commensal resident skin flora also known as the cutaneous microbiota. The microbiota lives a... Show more »

The human skin serves as the first natural physical barrier against external assaults while concomitantly providing a home for more than 1000 billion beneficial microorganisms. These microorganisms form the commensal resident skin flora also known as the cutaneous microbiota. The microbiota lives a symbiotic relationship with its host and interacts constantly with the adaptive immune system, thus creating a complex ecosystem which is unique to each individual but highly variable depending on several factors such as (temperature, ph, humidity, body area, life period, diet, etc.). This fragile balanced ecosystem helps to maintain healthy skin and protects it against the adhesion and development of pathogens. Its deterioration or imbalance can cause skin disorders (dry skin, oily skin, etc.) and amplify pathologies (psoriasis, atopic dermatitis, acne, etc.)
Thanks to its expertise in cellular and tissue engineering, Bioalternatives has developed models that are suitable for analyzing the interactions between microbiota and skin and evaluate the efficacy of your products from in silico to clinical sample bioanalysis.


Microbiota-friendly studies:
- Bacterial growth and yeast growth: Photometry (OD), Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM)
- Bacterial adhesion on RHE surface: Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM), Radioactivity, Scanning electron microscopy)
- Bacterial & yeast quantification & identification:  Traditional microbiology on agar, Targeted qPCR, Non targeted metagenomic (MiSeq)


Anti-microbial studies:
- Biological target, active molecule: SELNERGY
- Bacterial viability, Bacterial growth /Yeast growth, Bacterial identification: Photometry (OD) - MIC / MBC / TKC, Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM)
- Bacterial adhesion on RHE surface, epidermal protein analysis (antimicrobial peptides), inflammation assay (cytokine release): Colony-forming unit (CFU), qPCR (SYBR Green & TaqmanTM), Radioactivity, Scanning Electron Microscopy
- Wound healing delay after bacterial infection: Wound diameter, tissue morphology (HES)
- Bacterial quantification, bacterial identification: Traditional microbiology on agar, targeted qPCR, non targeted metagenomic (MiSeq).


Bioalternatives offers a wide range of microorganisms tested by our laboratory and available for in vitro testing:
GRAM (+)
Staphylococcus aureus

Staphylococcus epidermidis
Cutibacterium acnes
Corynebacterium xerosis
Streptococcus pyogenes
Mix of several strains (upon request)
Other strains upon request
GRAM (-)
Pseudomonas aeruginosa
Other strains upon request
VIRUSES
Herpes simplex virus type 1 (HSV-1)
Human Rhinovirus 16 (HRV-16)
YEAST
Malassezia


Example of Bioalternatives assays:
- Bacterial enumeration
The purpose of our test is to evaluate the effect of the compounds (molecules, active ingredients, extracts, etc.) on stimulating or inhibiting bacterial proliferation. This is done by bacterial enumeration using qPCR (DNA quantification) or by bacterial counting using colony forming unit (CFU). Products can be tested at different concentrations and on different time-point.
- RHE, adhesion of a bacterial mix (basal)
The purpose of our test is to evaluate via qPCR quantification the effect of compounds (molecules, active ingredients, extracts, etc.)  on the adhesion of a mix of 4 bacterial strains on reconstructed human epidermis.
(This test can also be realized with an uncial strains)
- NHDF, Cytokine/chemokine release
The purpose of our test is to evaluate via ELISA quantification the effect of compounds (molecules, active ingredients, extracts, etc.)  on the inflammatory response induced by bacterial strain in human fibroblasts.
(This test can also be realized in human sebocytes cell line)
- And more other tests


Other additional information (graphics, illustrations) are also provided on our website
Contact us!

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Microbiology Dermatology

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Anti-Inflammatory Drug Screening in Human Cells

Price on request

Inflammation is a biological response of the immune system that can be triggered by a variety of factors, including pathogens, damaged cells and toxic compounds. Our laboratory can support anti-Inflammatory project in the following research areas:


Cell and tissue damage:
Acute inflammation or... Show more »

Inflammation is a biological response of the immune system that can be triggered by a variety of factors, including pathogens, damaged cells and toxic compounds. Our laboratory can support anti-Inflammatory project in the following research areas:


Cell and tissue damage:
Acute inflammation or stress: innate immunity & epithelial barrier response against damage
Defense mechanisms
Chronic inflammation, stress or disease: tissue response, damage, ageing, etc.

Regulation of immune and inflammatory responses:
Recruitment, proliferation, differentiation, polarization and function of immune cells
Cytokine, chemokine and growth factor release

Tissue Regeneration:
Tissue repair, wound healing
Regeneration, proliferation and migration
Differentiation process
Function and recovery


Bioalternatives cells and tissues models:

Our immune blood cells
Primary cells (human)
Whole blood
PBMC
PMN
CD4+ T lymphocytes
CD19+ B lymphocytes B
CD14+ monocytes, M1 and M2 macrophages
Dendritic and langerhans cells
Granulocytes
Basophils
Primary cells (murin)
Splenocytes
Thymocytes
Mast cells
Cell lines
U937
THP1
HL-60
Jurkat


Readout:
-Macrophage-like U-937 (basal or stimulated)
IL-1 beta release
IL-6 release
IL-8 release
TNF- alpha release
Annexin V / PI
PGE2 release
TXB2 release
-Thymocytes (basal or stimulated)
Active caspase 3
-THP-1 (basal or stimulated)
Caspase-1 activity
IL-1 beta release
-T cells, (basal or stimulated)
IFN- gamma release
CD25 expression
-PMN (basal or stimulated)
IL-8 release
IL-1 release beta
CD11b/CD62L expression
-PBMc (basal or stimulated)
IFN- gamma release
PI3K/AKT activation
Cytokine release
Inflammation genes
-Mast cells (basal or stimulated)
Histamine release 
-HL-60
mRNA expression
Arachidonic acid metabolite release
-CD4+ T cells
Cytokine release
-CD19+ B cells
IL-6 release
Ig production


Example of Bioalternatives assays:
- Blood, human basophil activation assay
Immediate-type hypersensitivity is characterized by allergic reactions following the contact with an allergen. This allergic reaction can be mediated or not by immunoglobulin E (IgE) class of antibodies. The cross-linking of two IgE receptors (FceRI) by an allergen induces intracellular signaling pathways, thus resulting in the basophil activation. The ultimate step of basophil activation is the degranulation resulting in histamine release. Such histamine release is responsible for the appearance of allergy reactions.
The degranulation step consists in the fusion of cytoplasmic granules with the basophil plasma membrane. CD63 is then exposed to the extracellular matrix and it is considered as a specific marker of activated basophils.
The purpose of our test is to evaluate via flow cytometry the effect of compounds (molecules, active ingredients, extracts, etc.) on CD63 expression in CCR3+ basophils.
- Mast cells degranulation, histamine release (compound 48/80 stimulation)
Mast cells and basophils represent the most relevant source of histamine in the immune system. Histamine is stored in cytoplasmic granules. Histamine release is regulated by several activating and inhibitory receptors. In conventional allergy, presence of allergen specific IgE activate mast cell degranulation and histamine release.
The purpose of our test is to evaluate via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.) on histamine release in rat stimulated mast cells.
- Peripheral Blood Mononuclear Cells stimulated monocytes, cytokine release (LPS or anti-CD3/anti-CD28 stimulation)
The purpose of our test is to evaluate via flow cytometry (multiplex) the effect of compounds (molecules, active ingredients, extracts, etc.)  on cytokine in stimulated Peripheral Blood Mononuclear Cells (PBMC).
- And more other tests


Other additional information (graphics, illustrations) are also provided on our website
Contact us!

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In vitro Acne Models

Price on request

Acne is a chronic inflammatory pathology of the pilosebaceous follicle under hormonal dependence that includes:
-abnormal increase of sebaceous lipid production (sebum)
-formation of microcomedones due to an abnormal epidermal differentiation, a likely consequence of abnormal androgen (testosterone)... Show more »

Acne is a chronic inflammatory pathology of the pilosebaceous follicle under hormonal dependence that includes:
-abnormal increase of sebaceous lipid production (sebum)
-formation of microcomedones due to an abnormal epidermal differentiation, a likely consequence of abnormal androgen (testosterone) metabolism
-accumulation of peroxidized lipids on the surface (blackheads) or inside the comedo (whitehead)
formation of infectious and non-infectious inflammatory lesions and a skin wound healing process


Bioalternatives provides you with a range of innovative in vitro pharmacological models and assays, more particularly dedicated to the evaluation of the pharmacological efficacy (screening, profiling, proof-of-concept) of your products (APIs, biosimilars, fomulations, medical devices):
-Sebaceous gland regulation and hyperseborrhea
-Androgen metabolism
-Abnormal epidermal differentiation
-Lipid peroxidation
-Inflammatory, lesional or microbial response
-Infection, immune system and skin defenses


Bioalternatives cells and tissues models
-Human sebocytes: SEBO662 and SEBO662AR cell lines
-Normal human epidermal keratinocytes (NHEK)
-Normal human dermal fibroblasts (NHDF)
-Human follicle dermal papilla cells (HFDPC)
-Reconstructed human epidermis (RHE)


Readout:
-SEBO662 (basal)
testosterone metabolism
5- alpha reductase activity
-SEBO662AR (basal or stimulated)
Lipogenesis
-NHEK (Basal)
Antimicrobial peptide


Example of Bioalternatives assays:
- Keratinocytes antimicrobial peptide release
Elafin and beta-defensin 2 are potent antimicrobial peptides which are notably expressed in normal human keratinocytes. A high level of expression of these two peptides in keratinocytes is frequently associated with inflammatory skin lesions.
The purpose of our test is to evaluate via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.)  on peptide release (beta-defansin, elafin) in human keratinocytes.
- Sebocytes, testosterone metabolism & 5-a reductase activity
Acne is a multiparametric skin disorder in which sebum hypersecretion induced by circulating testosterone is involved. Testosterone is converted, by 5α-reductase, into dihydrotestosterone (DHT), one of its biologically active metabolites. Moreover, an excess of sebum (hyperseborrhea) also favors the development of bacteria (i.e. Propionibacterium acnes) which in turn induces inflammation. The inhibition of 5α-reductase enzymatic activity and/or inflammation is known to reduce acne in human skin.
The purpose of our assays is to evaluate, via [14C]-testosterone incorporation, the effect of compounds (molecules, active ingredients, extracts, etc.) on 5-alpha reductase activity in human sebocytes 
- Sebocyte, lipogenesis
Acne is an inflammatory pathology linked to an abnormal production of sebum by the sebocytes. Sebum is produced because of a complex process of lipid synthesis, lipogenesis and then released by holocrine secretion. The production is under hormonal control such as testosterone known to stimulate the production of sebum. The stimulation by a complete testosterone results in an even stronger increase of the lipid synthesis and storage.
The purpose of our assays is to evaluate, via fluorescence, the effect of compounds (molecules, active ingredients, extracts, etc.) on lipid production in a model of human sebocytes cell line
- And more other tests


Other additional information (graphics, illustrations) are also provided on our site on our website
Contact us!

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Fluorescent labeling PCR Immunofluorescence

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In vitro Psoriasis Models

Price on request
Psoriasis is a non-contagious inflammatory skin disease linked to a genetic predisposition and which develops under the influence of environmental parameters. While no treatment can completely cure this autoimmune disease, psoriasis is the subject of intensive research and especially so since the identification of... Show more »
Psoriasis is a non-contagious inflammatory skin disease linked to a genetic predisposition and which develops under the influence of environmental parameters. While no treatment can completely cure this autoimmune disease, psoriasis is the subject of intensive research and especially so since the identification of subpopulations of Th17 and Th22 lymphocytes.

Bioalternatives has carried out an extensive research program on psoriasis  and can offer a full range of innovative models and in vitro pharmacology assays specifically dedicated to the evaluation of pharmacological efficacy (screening, profiling, proof-of-concept) of your products (APIs, biosimilars, formulations, medical devices):
-Immune response (Th17/Th22 cytokine release)
-Induction in keratinocytes (or reconstructed epidermis) of a psoriasis-like profile by cytokines
-Production of chemokines, antimicrobial peptides (AMPs) and expression of epidermal differentiation markers
-Specific marker analysis by immunoassays or RT-qPCR

Bioalternatives cells and tissues models:
Reconstructed Epidermis, Bioalternatives (RHE)
Normal Human Epidermal Keratinocytes (NHEK)
CD4+ T cells

Readout:
-RHE (basal or stimulated):
S100A7
Beta-defensin 2/4
RNAse7
-NHEK (stimulated):
STAT3 activation
NF kappaB translocation
-CD4+ T cells (stimulated):
IL-17 release

Example of Bioalternatives assays:
- Keratinocyte, gene expression (cytokine stimulation)
The purpose of our assays is to evaluate, via qPCR, the effect of compounds (molecules, active ingredients, extracts, etc.) on gene implicated in psoriasis expression in normal human keratinocytes. A mix of pro-inflammatory cytokines is used to stimulate the cells in order to recreate the pathological tissue environment found in the chronic phase of psoriasis.
(This test can also be realized in Reconstructed Human Epidermis (RHE))
- Kératinocytes antimicrobial peptide release (cytokine stimulation)
The purpose of our assays is to evaluate, via ELISA, the effect of compounds (molecules, active ingredients, extracts, etc.) on peptides implicated in psoriasis expression (beta defebsin 2, S100A7, elafin) in normal human keratinocytes. A mix of pro-inflammatory cytokines is used to stimulate the cells in order to recreate the pathological tissue environment found in the chronic phase of psoriasis
- Reconstructed Human Epidermis), skin innate immunity markers expression (cytokine stimulation)
The purpose of our assays is to evaluate, via immunohistochemistry or immunohistofluorescence, the effect of compounds (molecules, active ingredients, extracts, etc.) on skin innate markers implicated in psoriasis expression (beta defebsin 2/4, S100A7, RNAse 7) in normal human keratinocytes. A mix of pro-inflammatory cytokines is used to stimulate the cells in order to recreate the pathological tissue environment found in the chronic phase of psoriasis


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Gene expression profiling PCR ELISA Flow cytometry western blot histology Immunofluorescence immunohistochemistry Human Show 9 more tags Show less

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In vitro Wound Healing Assay

Price on request
Wound healing is a complex and dynamic process of restoring skin cellular structures and tissue layers that involves multiple components: differentiated cells, stem cells, hair follicles, extracellular matrix (ECM) proteins, cytokines networks, microRNAs , blood vessels, nerves  and mechanical forces.
There is... Show more »
Wound healing is a complex and dynamic process of restoring skin cellular structures and tissue layers that involves multiple components: differentiated cells, stem cells, hair follicles, extracellular matrix (ECM) proteins, cytokines networks, microRNAs , blood vessels, nerves  and mechanical forces.
There is 4 interrelated and overlapping phases:
-haemostasis,
-inflammation,
-granulation/proliferation
-maturation/remodelling

Thanks to its expertise Bioalternative can evaluate the pro-healing effect of active compounds, formulations or medical devices by measuring:
-inflammatory response, cleaning of skin wounds and innate immunity
-reepithelialization
-neovascularization and neuritogenesis
-extracellular matrix synthesis, fibrosis and myofibroblast interaction
-dermal remodeling and epidermal differentiation
-pigmentation

Bioalternatives cells and tissues models:
-Cell lines
-Peripheral blood immune cells (PBMCs and purifed blood cell populations: subpopulations of T lymphocytes, monocytes, polymorphonuclear cells, etc.)
-Macrophages and monocyte-derived dendritic cells
-Normal human epidermal keratinocytes (NHEK)
-Normal human dermal fibroblasts (NHDF)
-Myofibroblasts
-Mesenchymal stem cells
-Microvascular endothelial cells
-Normal human epidermal melanocytes (NHEM)
-Sensory neurons
-Reconstructed human epidermis (RHE)
-Dermal equivalents (DE)
-Skin explants (ex vivo)

Example of Bioalternatives assays:
- Fibroblast, IL-8 release (IL-1a stimulation)
The purpose of our assays is to evaluate, via ELISA, the effect of compounds (molecules, active ingredients, extracts, etc.) on IL-8 release in normal human keratinocytes stimulated with IL-1-alpha.
- Keratinocytes, migration/proliferation
Epidermal regeneration is a complex and dynamic process responsible for restoring the cellular and structural components of the skin, using the mechanisms of keratinocyte proliferation and migration as well as extracellular matrix remodeling.
The purpose of our test is to evaluate via fluorescence/imaging the capacity of a compound to stimulate the cell migration process, using the Oris™ Cell Migration assay in normal human keratinocytes.
- Keratinocyte, differentiation markers
Bioalternatives has developed an assay to determine keratinocyte differentiation on Normal Human Epidermal Keratinocytes cultivated in low calcium medium.
The purpose of our in vitro assay is to evaluate the compound capacity to modulate the expression of early (K1, K10, involucrin….) or later (Loricrin, Transglutaminase, Filagrin…) differentiation markers on normal human keratinocytes, by using Rt-qPCR.   
- And more other tests

Other additional information (graphics, illustrations) are also provided on our site on our website
Contact us!
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Immunofluorescence Flow cytometry ELISA PCR

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Claim Substantiation

Price on request

Are you looking for solutions to support your claims? in vitro assays are highly effective for establishing proof-of-concept of your compounds (e.g. extracts, active ingredients, formulations).

BIOalternatives, a premier in vitro dermatological testing service laboratory, can assist you at every step of the pre-clinical... Show more »

Are you looking for solutions to support your claims? in vitro assays are highly effective for establishing proof-of-concept of your compounds (e.g. extracts, active ingredients, formulations).

BIOalternatives, a premier in vitro dermatological testing service laboratory, can assist you at every step of the pre-clinical development of your products: screening of bioactive compounds, proof-of-concept studies, repositioning of active ingredients.

Our knowledge of skin biology and its application to new target assessment, mechanistic
understanding and claim substantiation are without question of the highest standards in the industry :

  • Epidermal regeneration
  • Skin barrier and hydration
  • Skin pigmentation
  • Skin aging
  • Skin protection
  • Skin vascularization
  • Skin inflammation
  • Sebaceous gland regulation
  • Adipocyte metabolism
  • Hair regeneration
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In vitro Skin Models

Price on request
We provide a wide range validated assays for compound (ingredient or finish products) testing using:
-2D skin models
Normal human epidermal keratinocytes (NHEK)
Normal human dermal fibroblasts (NHDF)
Normal human epidermal melanocytes (NHEM)
Human sebocytes SEBO662... Show more »
We provide a wide range validated assays for compound (ingredient or finish products) testing using:
-2D skin models
Normal human epidermal keratinocytes (NHEK)
Normal human dermal fibroblasts (NHDF)
Normal human epidermal melanocytes (NHEM)
Human sebocytes SEBO662 and SEBO662AR cell lines
etc.
-3D-skin related models
dermis equivalent, (DE)
reconstructed human epidermis (RHE)
reconstructed human epidermis including melanocytes (RHEm)
full thickness skin model
3D sebocytes
etc.
-Ex vivo skin models

Other additional information (graphics, illustrations) are also provided on our site on our website
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Dermal Pharmacology & Efficacy Studies

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Bioalternatives developed and validated in vitro cell and tissue models (e.g. normal or aged dermal fibroblasts, full thickness skin) and assays for investigating the anti-aging effect of cosmetics compounds, such as:
-cell migration
-proliferation or differentiation assays
-extracellular matrix synthesis or... Show more »

Bioalternatives developed and validated in vitro cell and tissue models (e.g. normal or aged dermal fibroblasts, full thickness skin) and assays for investigating the anti-aging effect of cosmetics compounds, such as:
-cell migration
-proliferation or differentiation assays
-extracellular matrix synthesis or degradation assays (e.g. collagen or tropoelastin synthesis/ expression, MMP activities)
-gene expression assays
-reinforcement of dermis cohesion and firmness
-production of extracellular matrix components and improvement of cell-matrix interactions (collagen I, collagen III), elastin, fibronectin, proteoglycans, glycoaminoglycans  and hyaluronic acid proteases (MMPs) etc.).
-improvement of dermo-epidermal junction quality and cell adhesion (integrins, laminin V, collagen IV, collagen VII, desmogleins, etc.).


Bioalternatives cells and tissues models
-normal human dermal fibroblasts (NHDF)
-dermal equivalent (DE)
-normal human epidermal keratinocytes (NHEK)
-reconstructed human epidermis (RHE)
-full thickness reconstructed skin (FTSK)
-skin explants (ex vivo)

Example of Bioalternatives assays:
- Dermal equivalent, dermis strength and cohesion
The purpose of our assay is to evaluate via imaging the effect of compounds (molecules, active ingredients, extracts, etc.) on lattice contraction in dermis equivalent.
- Fibroblast, procollagen I synthesis/release
In the dermis, fibroblasts continuously secrete the precursors of all the components of the extracellular matrix, thus maintaining the skin structural integrity. The extracellular matrix is a complex structure formed of a very well-organized network of collagen fibers, elastic fibers and reticular fibers. Fibril collagens are the most abundant proteins present in human skin, creating more than 90% of its dry weight. Type I collagen represent 60 to 80% of collagens of the dermis and the hypodermis. Type I, II and V fibril collagens self-assemble in thicker fibers forming a tridimensional network in the entire thickness of the dermis. They give to the skin its resilience strength and are essential to the integrity of the tissue. Collagen network is organized and maintained under dynamic mechanical tension provided by fibroblasts responsible of its production.
The purpose of our assay is to evaluate via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.) on Procollagen I synthesis / release in normal human fibroblasts.
- Fibroblast, extracellular matrix gene expression
 The purpose of our assay is to evaluate, via RT-qPCR  technology, the capacity of compounds to modulate the gene expression profile of normal fibroblasts.  Extracted mRNA is analyzed on a dedicated PCR array (mQPA H-NHDF-16 or 64) designed by Bioalternatives and containing 16 or 64 target genes (including housekeeping genes) selected for their importance in the fibroblast physiology and the synthesis or degradation of Extracellular matrix.
- And more other tests
Other additional information (graphics, illustrations) are also provided on our site on our website
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qPCR primary cell culture ELISA Immunofluorescence Human Aging Immunology Show 7 more tags Show less

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In vitro Hair Growth Model

Price on request
Hair is a unique feature of each individual and, more generally, of the human species. It is estimated that adults have about 5 million hair follicles, with 1 million hair follicles found on the head, and with only 120,000 to 150,000 hair follicles covering the scalp. Depending on the region of the scalp, their density... Show more »
Hair is a unique feature of each individual and, more generally, of the human species. It is estimated that adults have about 5 million hair follicles, with 1 million hair follicles found on the head, and with only 120,000 to 150,000 hair follicles covering the scalp. Depending on the region of the scalp, their density varies by 200 to 300 follicles /cm2. A hundred hair follicles are naturally shed each day. The speed at which hair grows is about 0.3 mm per day or 12 cm per year. This speed is affected by numerous factors, such as follicle location, gender, age and ethnicity of the individual as well as environmental factors.

Bioalternatives developed and validated an ex-vivo model of human hair follicle suitable for investigating the effect of compounds on:
-the stimulation/ inhibition of hair growth
-the survival rate increase in cell cultures (anti apoptosis, anti-hair loss focus)
-the hair pigmentation.
-the increase in cell viability in culture (anti-apoptosis, anti-hair loss)
-the increase of the hair shaft diameter
-5-alpha reductase activity (androgen metabolism)
-specific marker expression (Ki67, TGK, fibronectin, β-catenin, cytokines etc.)

These parameters can be analyzed by morphometric techniques (hair elongation and viability measurement) but also through complex molecular approaches:
- gene expression (dedicated PCRarrays or full genome profil)
- immunohistochemistry or histology as special staining e.g. Fontana Masson for pigmentation.

Bioalternatives cells and tissues models
-human follicle dermal papilla fibroblasts (HFDPC)
-human hair follicle (ex vivo)

Readout:
- Hair follicle:
Protein expression (Ki67, K15, K19, TGK, collagen 1, fibronectin, etc.).
Gene expression
- HFDPC:
Testosterone metabolism
5-alpha reductase activity

Example of Bioalternatives assays:
- Hair follicle growth & degeneration
Our ex vivo hair follicle model is directly derived from the widely recognized standard conditions described by Philpott et al. It represents a “natural” co-culture model of human cutaneous cells (keratinocytes, fibroblasts, melanocytes and stem cells).
The purpose of our assay is to evaluate the stimulating or inhibiting effects of compounds (molecules, active ingredients, extracts, etc.) on hair growth or hair survival (anti-apoptosis, anti-hair loss) by using morphometric techniques (images and measurements).
- Human Follicle Dermal Papilla Cells, testosterone metabolism & 5-a reductase activity
The conversion of testosterone to 5-dihydrotestosterone (DHT), the active metabolite of testosterone, by the 5α-reductase enzyme is one of the causes of sebaceous gland hypersecretion and of premature hair loss.
The purpose of our assay is to evaluate the effects of compounds (molecules, active ingredients, extracts, etc.) on the 5-α reductase activity of human hair follicle dermal papilla fibroblasts (HFDPC). This assay is based on the incorporation of [14C]-testosterone and on the extraction and separation/radiodetection of the formed metabolites. In particular, it allows the production of the active metabolite of testosterone (dihydrotestosterone) to be measured.
- Hair follicle, protein expression
Hair follicles are located under the skin and are divided into two main parts: one is of epithelial origin and is composed of the matrix (reserve of highly replicative matrix cells essential for hair follicle growth), the shaft, the internal root sheath and the external epithelial root sheath made of keratinocyte filaments; the other part is of mesenchymal origin and comprises the connective tissue sheath made of collagen and proteoglycans and the dermal papilla (reserve of fibroblasts) which is separated from the epithelial compartment by the basement membrane.
The purpose of our assay is to evaluate the stimulating or inhibiting effects of compounds (molecules, active ingredients, extracts, etc.) on hair follicles in culture with regard to the expression and/or localization of relevant proteins (e.g., Ki67, K15, K19, TGK, collagen I, fibronectin, etc.) by immunohistochemistry or immunofluorescence.
- And more other tests

Other additional information (graphics, illustrations) are also provided on our site on our website
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Alopecia Hypertrichosis Hirsutism microscopy qpc immunohistochemistry histology Immunofluorescence ELISA Human Show 10 more tags Show less

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Angiogenesis & Endothelial Cell Assays

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Dermal arterioles and venules are composed of endothelial cells, connective tissues (collagen, elastin) and of smooth muscle cells which allow vessel vasoconstriction and vasodilation. Dermal capillaries are composed of a single layer of endothelial cells and of pericytes surrounded by a basal... Show more »

Dermal arterioles and venules are composed of endothelial cells, connective tissues (collagen, elastin) and of smooth muscle cells which allow vessel vasoconstriction and vasodilation. Dermal capillaries are composed of a single layer of endothelial cells and of pericytes surrounded by a basal membrane.
Angiogenesis involves the formation of capillaries from preexisting microvessels and therefore contributes to vascular remodeling and maturation. It plays a pivotal function in a variety of normal and pathological conditions such as embryonic development, the menstrual cycle, hair cycle, wound healing, arthritis, psoriasis, proliferative diabetic retinopathy, atherosclerosis, post ischemic vascularization of the myocardium and tumor growth and metastasis.
Microcirculation can be altered by external factors and also skin ageing, with a progressive decrease of new blood vessel formation.


Bioalternatives can evaluate the effect of active cosmetic compounds on skin microvascularization by measuring:
-Viability, proliferation and migration of endothelial cells
-Cell differentiation and pseudotube (angiogenesis, neovascularization) formation
-Protection of endothelial cells against stress (inflammation, infection, etc.) 
-Veinotonic activity of smooth muscle cells
-Angiogenic factor release (e.g.VEGF)


Bioalternatives cells and tissues models
-Human umbilical vein endothelial cells (HUVEC)
-Human dermal microvascular endothelial cells (HMVEC-d)
-HMVEC-d and NHDF coculture (ENDOF)
-Human aortic smooth muscle cells (AoSMC)
-Other skin cells (NHEK, NHDF, etc.)

Readout
-NHDF:
VEGF release
-HUVEC:
ICAM-1 expression


Example of Bioalternatives assays:
- Differentiation/pseudotube formation (VEGF stimulation, anti-angiogenic agents)
Angiogenesis involves the formation of capillaries from preexisting microvessels and therefore contributes to vascular remodeling and maturation. It plays a pivotal function in a variety of normal and pathological conditions such as embryonic development, the menstrual cycle, hair cycle, wound healing, arthritis, psoriasis, proliferative diabetic retinopathy, atherosclerosis, post ischemic vascularization of the myocardium and tumor growth and metastasis. The initiation of the physiopathological angiogenic response, known as the ‘angiogenic switch’, depends on the dynamic balance between exogenous or endogenous stimuli (pro-angiogenic factors) and inhibitors (anti-angiogenic factors) acting in the immediate environment of endothelial cells.
The in vitro assay proposed below consists in a co-culture model of normal human endothelial cells with normal human dermal fibroblasts. After several days of incubation, the treatment of the co-culture with the pro-angiogenic factor VEGF results in the organization of endothelial cells in pseudotubes. Therefore, under VEGF-stimulated condition, this model is suitable to evaluate test compounds capable of inhibiting the formation of this pseudotube network. The pseudotube formation in this model is analyzed by image analysis further to the specific immunolabelling of endothelial cells only using an anti-VWF antibody.
Remark : Under basal conditions (in absence of VEGF), our co-culture model can also be used to screen compounds capable of promoting the development of the pseudotube.
- Veinotonic activity
Vascular smooth muscle cells play an essential role on the microvascular system and most vasoconstrictors/ veinotonic exert their effects by acting directly on the smooth muscle cells of the vascular wall. The contraction of smooth muscular cells, in most cases linked to an increase of cytoplasmic calcium, causes the vasoconstriction of nearby blood vessels.
The purpose of this assay is to evaluate, via time-lapse fluorescence microscopy, the capacity of the compounds to stimulate the contraction of primary smoth vascular muscle cell by measuring their capacity to induce the mobilization of intra-cellular calcium.
- VEGF release
The purpose of our assay is to evaluate, via ELISA the effect of compounds (molecules, active ingredients, extracts, etc.) on VEGF release in normal human fibroblast.

- And more other tests


Other additional information (graphics, illustrations) are also provided on our site on our website
Contact us!


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Cell and Tissue Culture

Price on request

Cell and tissue engineering, development of new comple in vitro mammalian models:

  • Tissue microdissection
  • Primary cell isolation
  • Cell line construction
  • Co-culture model system
  • Organotypic explant culture
  • Three dimensional reconstruction
  • Phenotypic and functional characterization

Cell and tissue engineering, development of new comple in vitro mammalian models:

  • Tissue microdissection
  • Primary cell isolation
  • Cell line construction
  • Co-culture model system
  • Organotypic explant culture
  • Three dimensional reconstruction
  • Phenotypic and functional characterization
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Cell-Based Assays

Price on request

Cellular and molecular pharmacology assays for hit-to-lead selection of active pharmaceutical ingredients and claim substantiation of healthcare products:

  • cell-based assays
  • Screening
  • Profiling
  • Proof-of-concept
  • Mechanism of action
  • Benchmarking
  • Safety
  • Testing of formulations prior clinical trial

Cellular and molecular pharmacology assays for hit-to-lead selection of active pharmaceutical ingredients and claim substantiation of healthcare products:

  • cell-based assays
  • Screening
  • Profiling
  • Proof-of-concept
  • Mechanism of action
  • Benchmarking
  • Safety
  • Testing of formulations prior clinical trial
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Assay Development

Price on request

Services for the development, customization and optimization of innovative research methods and protocols :

  • Literature review
  • Feasability study
  • Protocol design
  • Method validation
  • Multiplexing, miniaturization and optimization

Services for the development, customization and optimization of innovative research methods and protocols :

  • Literature review
  • Feasability study
  • Protocol design
  • Method validation
  • Multiplexing, miniaturization and optimization
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ELISA Flow cytometry western blot Immunoassays Immunofluorescence immunohistochemistry histology qPCR Fluorescent labeling radiochemistry Biochemistry Show 11 more tags Show less

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Bioanalysis

Price on request

Biological sample collection, processing, storage and information management:

  • Study design
  • Sample Collection
  • Method develoment and validation (tissue microarrays, cDNArrays, PCRarrays, immunoassays, biochemical assays)
  • Analysis service
  • Biobanking (RNA, cDNA, cells, frozen tissues, embedded tissues)
  • Data management

Biological sample collection, processing, storage and information management:

  • Study design
  • Sample Collection
  • Method develoment and validation (tissue microarrays, cDNArrays, PCRarrays, immunoassays, biochemical assays)
  • Analysis service
  • Biobanking (RNA, cDNA, cells, frozen tissues, embedded tissues)
  • Data management
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Cell Migration Assays

Price on request

Cell migration is commonly assessed using the scratch assay which measures the movement of cells into an artificial wound made on a confluent cell monolayer to create a cell-free area. As an alternative to the scratch assay which has high variability, the Oris™ Cell Migration Assay allows the formation of homogeneously sized... Show more »

Cell migration is commonly assessed using the scratch assay which measures the movement of cells into an artificial wound made on a confluent cell monolayer to create a cell-free area. As an alternative to the scratch assay which has high variability, the Oris™ Cell Migration Assay allows the formation of homogeneously sized cell-free areas into which migration can occur without releasing factors from dead cells or damaging the underlying extracellular matrix.

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Cell Proliferation Assays

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BrdU Cell Proliferation assay or 3H thymidine incorporation assay

BrdU Cell Proliferation assay or 3H thymidine incorporation assay

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In vitro Reporter Gene Assays

Price on request

Cell-Based Gene Expression Assays using qPCR (SYBR Green or TaqMan) or microrrays (Affymetrix);

Cell-Based Gene Expression Assays using qPCR (SYBR Green or TaqMan) or microrrays (Affymetrix);

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LightCycler Affymetrix GeneAtlas system

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Human Skin Acute Inflammation Assays

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Antiviral Testing

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Cytokine Analysis

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ELISA Flow cytometry

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Drug Mechanism of Action Studies

Mechanism of Action Studies
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Cells and Tissues

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Medicinal Chemistry

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Cell Invasion & Migration Assays

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Biology

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Functional Human Tissue Assays

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Drug Discovery

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Project Management

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In vitro Disease Models

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Product Development, Testing, and Packaging

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Drug Discovery & Development

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Cell Viability & Proliferation Assays

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Safety Pharmacology

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Immunoassays

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Cell-Based Compound Screening

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Compound Profiling

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In vitro Immunogenicity Assays

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Omics

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Guillaume Tenca

Sales Manager
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Maxance Vandevyvere

Business Developer, Legal & IP Manager

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