Bio S&T is your resource for all of your DNA and cDNA library construction and screening needs. Located in Montreal, Canada, we have been able to retain the privilege of providing research services for almost all of the world’s top pharmaceutical, agriculture and biotech companies, as well as some of the most renowned universities and research institutes.
We are proud to offer our clients our flagship BAC library construction and screening service. Our clients in the crop, animal and microbial science community, have been extremely pleased with the many successful screening projects we have completed. We have provided the requisite clones containing gene sequences of interest such transgene insertion sites, small molecule biosynthetic gene clusters etc. Additionally, we offer BAC clone and genomic sequencing; fosmid and cosmid genomic library construction, normalized and subtracted cDNA library construction, as well as exclusive products used in retroviral research and genomic walking.
APAgene™ GOLD Genome Walking Services are designed to rapidly and reliably amplify unknown genomic DNA using our patented APA technology.
APAgene™ GOLD Genome Walking allows you to carry out an entire range of molecular genetic experiments:
Did you know that our APAgene™ GOLD Genome Walking technology was cited in Molecular Biotechnology (D. Cullen et al., 11 January 2011 pp. 1-13) where it was rated best in class? The technology was also cited in Food Chemistry (MA Fraiture et al, 15 March 2014 pp 60-69) as an approach in identifying unauthorized GMOs.
Bio S&T's high-quality RNA can be used directly for NxGen RNA sequencing as well as other downstream applications that require optimal-quality RNA such as quantitative real-time RT-PCR, microRNA cloning and amplification and Northern blotting.
Very competitive price is available upon request. We tailor the library to each scientist's needs.
Fosmid clones are similar in size (ca. 40kb) to cosmids, but, like BAC clones, they contain replicons derived from the F factor for DNA replication and segregation. Because of this property, they are present in low copy number and consequently more stable than cosmids. Also, the copy control vector can be used for easy DNA purification from fosmid clones.
Fosmid libraries are commonly used for closing gaps in a whole genome sequence, physical maping, metagenomic and expression screening and more. Although we greatly prefer to perform the DNA isolation at our facilities we understand that this is not always feasible. Fosmid libraries, contrary to BAC large-insert libraries, do not require HMW DNA (typically 2Mb) as a starting material. This allows scientiststhe needed flexibility in providing DNA to construct a library.
Proprietary high efficiency cloning methods guarantee high quality and genome coverage that customers need. Obtained inserts sizes are always uniform and range within 35-45Kb. Our optimized methods are reliable, fast and cost effective.
We offer non-arrayed libraries and arrayed libraries (colony picking, replicate plates, high density filters). We also offer customer library screening for positive clones. Copy control vector can be used for easy DNA purification from fosmid clones.
Bio S&T produces standard (non-normalized) and normalized cDNA libraries using proprietary technology which features full-length-enriched, high quality size-fractionated and directionally cloned cDNA.
Normalized libraries are characterized by a reduction of abundant transcripts (typically 100X fold). Additionally, our normalization hybridization method prevents significant loss of average insert size. We perform normalization of cDNA population prior to cloning and library preparation. This prevents size bias against long and otherwise difficult-to-amplify cDNA.
Available vectors for construction are pBluescript or pcDNA3.1. Cloning into a customer-provided vector can be performed (additional fee applies).
Bio S&T has been providing high-quality BAC libraries for almost 20 years. We focus on high quality and have tremendous experience with all types of starting material (especially plants, GC-rich organisms and marine bacteria). Custom libraries remain the customer’s property and are never used internally or re-sold.
We typically construct a guaranteed 5X BAC library. BAC libraries are typically constructed using pCC1BAC and partial enzymatic digestion. Other available vectors are pCC1BAC, pCLD04541 as well as shuttle vectors. HMW DNA isolation and QC by HMW DNA enzymatic are included. Our BAC libraries have a high average insert and even insert size distribution. Average insert size is dependent on the quality of the starting material but is typically greater than 120Kb. BAC clones are amplified on semi-solid media in individual Petri dishes by overnight culture. It is worth noting that semi-solid medium amplification is preferable to liquid amplification as it's best for representation. Pooled, arrayed, and non-arrayed libraries are offered.
Pooled libraries: BAC clones are pooled and distributed in 96-well plates. We distribute the primary BAC clones at a rate of about 300-500 clones/well (depending on genome size). The pooled library is then screened using customer-provided PCR primer pair(s).
Arrayed libraries: An arrayed library is the preferred choice for downstream applications such a full genome sequencing, physical mapping and screening via hybridization. BAC clones will be arrayed and amplified in 384-well plates (1 unique clone/well) and shipped on dry ice. Additional services offered are: small and large nylon membranes for hybridization; preparation of pools and superpools; duplicate plates; screening.
Non-arrayed libraries: A non-arrayed BAC library is ideal for scientists studying large-size genomes and/or having arraying capabilities and/or wishing to limit shipping fees. BAC clones (transformed E. coli cells containing BAC DNA) will be supplied as glycerol stock on dry ice.
Additional services offered: preparation of row and column pools (cell stock and/or DNA can be provided); duplicate plates; additional screening.
Bio S&T is the exclusive worldwide provider of pESAC13 PAC libraries. This unique vector (licensed exclusively to Bio S&T by NAICONS) is used to create Streptomyces sp. and Actinomycete sp. libraries. These libraries can then be screened for clones that will be positive for the desired gene clusters of biosynthetic pathways.
ESAC (E. coli-Streptomyces Artificial Chromosome) vectors are bacterial artificial chromosomes that can be shuttled between Escherichia coli, where they replicate autonomously, and a suitable Streptomyces host, where they integrate site-specifically into the chromosome.
pESAC13 is a derivative of pPAC-S1, one of the non-conjugative ESACs originally developed (Sosio et al., 2000). It was constructed by inserting a 760 bp DNA fragment containing oriT from the RK2 replicon (Simon et al., 1983) into the unique BstXI site of pPAC-S1 (M. Sosio et al., unpublished).
The important advantage of pESAC13 is that libraries can be conjugatively transferred from E. coli to a numberof Streptomyces species as well as into several non-streptomyces actinomycetes, without the need to purify large-insert clones and introduce them by protoplast mediated transformation or other means.
Bio S&T can clone large inserts ranging in size from 9-23 kb into a lamdba vector. The Lambda DASH II Vector (Stratagene) will be used for genomic lambda library construction. Instructions will be followed as specified by manufacturer (Stratagene). We do not guarantee average insert size but will attempt to obtain desired range of average insert size. Insert size can vary from about 9-23Kb, as specified in manufactures’ kit. We guarantee the minimum requested 1,000,000 clones.
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