Georgetown University Medical Center, we provide customized Surface Plasmon Resonance (SPR) services with a Biacore T-200 and a Biacore 4000. The two Biacore machines in our facility utilize SPR technology to study biomolecular binding events on a chip surface. The basic principle involves immobilization of a ligand on a sensor chip followed by delivery of an analyte by a microfluidic system. Any protein, DNA, RNA, lipid, carbohydrate, polysaccharide, cell, virus, drug, drug like molecule (organic or inorganic) can be used as the ligand or analyte. Since the detection system is based on measuring total mass on the sensor chip surface neither ligand nor analyte has to be tagged. In addition to identifying binding partners to a target molecule, SPR also provides quantitative data on:
The Biacore T-200 instrument can measure direct interactions between two molecules and calculate binding affinities and thermodynamic parameters of the interaction using Surface Plasmon Resonance (SPR). Biacore 4000 is optimized for higher throughput application where a small molecule library, fragment library or large collection of hybridoma clones can be screened for direct binding to target proteins.
User pays the cost of Biacore sensor chips plus the following machine usage fees:
For large projects we can provide a customized quote to cover the entire study.
The Biacore 4000 instrument can perform small molecule or fragment based screening studies to identify direct binders to target proteins. The instrument is also optimized for antibody screening and characterization. Biacore 4000 experiments are priced based on the number of targets, controls and the size of the library. Typical small library screens (~1500 compounds) cost ~$4000 for academic institutions ($10,000 for others) .
The Biacore 4000 instrument is optimized for antibody screening and characterization.
"I will certainly work with BMISR again. We like the way the experiments are designed and iterated until all details are ironed out. Experiments are performed in a timely fashion and with expertise."
"Working with BMISR has been very easy and highly productive. Dr. Uren has guided us on the best way to carry out and get the maximum out of the assays. They are very responsive and worked with us throughout the planning, execution and analysis phases of the experiment."
"Yes. They are very reliable, nice and efficient people."
"Customer service and support were excellent. The study was conducted in a timely manner. We received detailed report as well as raw data upon request."
"Aykut was very helpful during the planning phase of the work and did an excellent job of explaining the results of our study."
"Working with BMISR was a charm. Aykut was so patient while we prepared our samples! Communication was easy and very fast. Highly recommended! Albert Parisien Project Manager Technology Access Centre in Bio-Innovation La Cité Ottawa, Canada"
Biacore Molecular Interaction Shared Resource (BMISR) has not received any endorsements.