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Beth Israel Deaconess Medical Center

Boston Massachusetts
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Flow Cytometry Core (BIDMC)

Boston Massachusetts
No Reviews Available

Committed to meet all of the flow cytometry needs for the BIDMC and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cell sorting. It is continuously expanding with the newest software and... Show more »

Committed to meet all of the flow cytometry needs for the BIDMC and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cell sorting. It is continuously expanding with the newest software and machinery for both sort and analysis capabilities of up to 30 fluorescent parameters. The Flow Cytometry Core facility is available to:
answer technical questions;
assist you in setting up flow cytometry experiments;
assist you with acquiring, analyzing and interpreting your data;
assist in data presentation and data storage;
provide training on the four benchtop analyzers and on software.

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1359394176163 b2813ee3587f6568c12ed9079f54fb41

Confocal Imaging Core (BIDMC)

Boston Massachusetts
100% Positive Reviews
7 Orders Completed

The function of the Confocal Core at the Beth Israel Deaconess Medical Center (BIDMC) is to provide services in confocal microscopy, light microscopy and immunostaining for cells and tissues including:
Confocal microscopy
Brightfield and fluorescence microscopy
Immunoperoxidase... Show more »

The function of the Confocal Core at the Beth Israel Deaconess Medical Center (BIDMC) is to provide services in confocal microscopy, light microscopy and immunostaining for cells and tissues including:
Confocal microscopy
Brightfield and fluorescence microscopy
Immunoperoxidase and immunofluorescence staining

Equipment in the facility includes:
Zeiss LSM510 Upright Confocal System
Zeiss LSM 510 Inverted Live-cell Confocal System
Zeiss Axioimager M1 Epifluorescence and Brightfield microscope
Dell workstation with Volocity and LSM510 software

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1386024971067 c0cb627df3b1e81603999e9964b81dea

Preclinical MRI Core

Boston Massachusetts
No Reviews Available

The Preclinical MRI Core Facility offers instrumentation and expertise for a broad range of magnetic resonance imaging and spectroscopy applications for small animals, excised tissue and cell culture studies. Our facility specializes in live animal MR imaging providing... Show more »

The Preclinical MRI Core Facility offers instrumentation and expertise for a broad range of magnetic resonance imaging and spectroscopy applications for small animals, excised tissue and cell culture studies. Our facility specializes in live animal MR imaging providing multiparametric information which can be used to define cohorts for animal trials and monitor over time. The Preclinical MRI Core also focuses on imaging ex vivo specimens, providing non-destructive 3D high resolution imaging to inform histology or other processes. We provide fee for service within BIDMC and to outside investigators.

Currently Available:
• 9.4 Tesla 20 cm bore MR imaging system
• 4.7 Tesla 40 cm bore MR imaging system
• 8.5 Tesla 9 cm bore MR imaging system
• Gas anesthesia and animal monitoring facilities

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CVVR Cell Sorting Core

Boston Massachusetts
No Reviews Available

The Center for Virology and Vaccine Research Flow Cytometry Core offers sterile cell sorting and acquisition as well as dedicated staff for assistance with instrument operation, data analysis, and panel development. The facility has two BD LSR II cytometers capable of detecting up... Show more »

The Center for Virology and Vaccine Research Flow Cytometry Core offers sterile cell sorting and acquisition as well as dedicated staff for assistance with instrument operation, data analysis, and panel development. The facility has two BD LSR II cytometers capable of detecting up to 16 colors and a BD FACS Aria sorter capable of detecting up to 18 colors and sorting into a variety of plates and tubes. A dedicated Aria operator assists with all sort designs and runs all samples.

Lab certifications:
- GCLP
- Harvard COMS

Standard shipping requests:
All specimens arriving from outside of the CLS building must be transported in accordance with DOT/IATA regulations. In general, this will entail triple layer packaging. Specimens arriving from inside the CLS building should be inside a secondary container (e.g. bucket).

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Bioinformatics and Systems Biology

Boston Massachusetts
No Reviews Available

The analysis of high-throughput genomics, proteomics, metabolomics and epigenomics data is the most critical step in systematically understanding pathophysiology of a disease as well as identifying potential biomarkers. The analysis of OMICS data often presents a challenge to... Show more »

The analysis of high-throughput genomics, proteomics, metabolomics and epigenomics data is the most critical step in systematically understanding pathophysiology of a disease as well as identifying potential biomarkers. The analysis of OMICS data often presents a challenge to translational and basic science researchers. The mission of Bioinformatics and Systems Biology core is to provide expertise and infrastructure in designing, analyses and simulation of high-throughput OMICS data to answer underlying biological questions. The core support analysis of data from many next-generation sequencing assays including transcriptional quantification (RNA-Seq), protein-nucleic acid interactions (ChIP-Seq), global methylation, genotyping or variant analysis through genome sequencing. To support cutting edge research, a special emphasis was made on implementing/developing systems biology frameworks and models for integrative analysis of genomic, epigenomics proteomic, metabolomic, imaging and clinical data to identifying key molecules driving pathophysiology. In addition to analyses support, core also provides resources for data management and high performance computing.

Recent Publications

  • Jaíra Ferreira de Vasconcellos, Angelo Brunelli Albertoni Laranjeira, Paulo C Leal, Manoj K Bhasin, Priscila Pini Zenatti, Ricardo J Nunes, Rosendo A Yunes, Alexandre E Nowill, Towia A Libermann, Luiz Fernando Zerbini, José Andrés Yunes, SB225002 Induces Cell Death and Cell Cycle Arrest in Acute Lymphoblastic Leukemia Cells through the Activation of GLIPR1, PloS one 10 (8), e0134783
  • RC Starling, J Stehlik, DA Baran, B Armstrong, JR Stone, D Ikle, Y Morrison, ND Bridges, P Putheti, TB Strom, M Bhasin, I Guleria, A Chandraker, M Sayegh, KP Daly, DM Briscoe, PS Heeger, Multicenter Analysis of Immune Biomarkers and Heart Transplant Outcomes: Results of the Clinical Trials in Organ Transplantation‐05 Study, American Journal of Transplantation 2015/8/1
  • Khurram Owais, Thomas Huang, Feroze Mahmood, Jeffery Hubbard, Rabya Saraf, Amit Bardia, Kamal R Khabbaz, Yunping Li, Manoj Bhasin, Ashraf A Sabe, Frank Sellke, Robina Matyal, Cardiopulmonary Bypass Decreases Activation of the Signal Transducer and Activator of Transcription 3 (STAT3) Pathway in Diabetic Human Myocardium, The Annals of thoracic surgery 2015/7/28
  • Liang Zhang, Xiaoen Wang, Andrea J Bullock, Marcella Callea, Harleen Shah, Jiaxi Song, Kelli Moreno, Barbara Visentin, Douglas Deutschman, David C Alsop, Michael B Atkins, James W Mier, Sabina Signoretti, Manoj Bhasin, Roger A Sabbadini, Rupal S Bhatt, Anti-S1P Antibody as a Novel Therapeutic Strategy for VEGFR TKI-Resistant Renal Cancer, Clinical Cancer Research 21 (8), 1925-1934
  • Andrej Jedinak, Adam Curatolo, David Zurakowski, Simon Dillon, Manoj K Bhasin, Towia A Libermann, Roopali Roy, Monisha Sachdev, Kevin R Loughlin, Marsha A Moses, Novel non-invasive biomarkers that distinguish between benign prostate hyperplasia and prostate cancer, BMC cancer 15 (1), 259
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