BellBrook labs
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Madison, Wisconsin, US
BellBrook Labs is dedicated to providing the pharmaceutical industry with enabling screening tools to accelerate the discovery of more effective therapies. As a small, R&D-driven company, we identify areas where there is a strong need but a significant technical hurdle, and then we develop a solution. BellBrook Labs is focused on taking creative approaches to new product development and to continually improving the level of service provided to our customers. When you communicate with someone from BellBrook it will be immediately apparent that we share your passion for discovering more effective disease therapies and your respect for the complexity of biology. We have included names of BBL employees and direct phone numbers on this website. Please feel free to contact any of us with questions or suggestions regarding our products and technologies or how we might better serve your needs. *Transcreener® Biochemical Assay Technology* BellBrook’s proprietary Transcreener® HTS Platform was developed to move more new targets into HTS more rapidly and to streamline selectivity profiling within a target family. It relies on homogeneous fluorescent immunodetection of nucleotides, including ADP, UDP, GDP, AMP and GMP, that are formed by thousands of cellular enzymes. Among these are group transferase families, such as kinases and glycosyltransferases, that catalyze the covalent regulatory reactions that are central to cell signaling. Each Transcreener Assay provides a generic detection method for multiple enzyme families. This means that you can focus on evaluating new therapeutic strategies and optimizing lead molecules rather than on time-consuming assay development for individual targets. Since the introduction of the first Transcreener assays in 2005, the platform has been rapidly validated and adopted by pharmaceutical and biotechnology screening labs around the world.
Whether you want to screen 2M compounds or perform SAR on 20, you need a robust, reliable biochemical assay that has been optimized for your target.
Leveraging BellBrook’s assay development expertise will save you time and money. Your project will be handled by the same BellBrook scientists who developed the Transcreener assays and have optimized them for more than 100 different enzyme targets, including kinases, ATPase, GTPases, glycosyltransferases, ligases/synthetases, and phosphodiesterases. We will define the exact conditions needed for initial velocity detection of your enzyme with outstanding signal:noise; Z’ values of 0.7 to 0.9 are typical. We will tailor our efforts to meet your specific needs, for example minimizing consumption of limited reagents or enabling simultaneous screening for inhibitors and activators. And we will work fast. If your target enzyme is in good shape, we can generally complete the assay development within two to four weeks.
Assay Development Services Include:
Target Protein Sourcing/Production – If the target protein is available from the customer or a vendor, we will test it for purity and functional activity; if not, we will produce it using the most efficient and cost effective expression and purification strategy.
Assay Optimization with Target Protein – We will determine the optimal buffer composition, enzyme concentration, and reaction time and optimize detection reagents to produce the maximal signal under initial velocity conditions
Assay Validation – Depending on customer needs, validation could include dose responses with control inhibitors, determination of kinetic parameters, pilot screens with targeted libraries, or interference screens.
BellBrook scientists will use their extensive biochemistry and enzymology expertise to identify actives from your virtual screen, prioritize your HTS hits, or generate quantitative data to drive your SAR/medicinal chemistry efforts.
We will work closely with you to define the specific parameters that are important for your lead discovery program and implement the most efficient and cost effective assays and/or kinetic analyses.
Lead Discovery Services Include:
BellBrook Labs provides a variety of biochemical assay development and lead discovery services to advance your target into a screen faster and with confidence.
Assay Development Services Include:
Lead Discovery Services Include:
The Transcreener ADP Kinase Assay is a universal assay platform that relies on the direct detection of ADP to monitor kinase activity. As a homogenous assay, it can be used for high throughput screening. The platform has been validated in millions of wells for drug discovery.
Chose between a variety of services including:
Transcreener® ADP2 Assay
Transcreener® UDP2 Assay
Transcreener® GDP Assay
Transcreener® AMP/GMP Detection Assay
ENPP1 Assay Services
Interested in moving your program forward, but don't want to bring an assay in-house? Our scientists can help! BellBrook scientists will use their extensive biochemistry and enzymology expertise to work with you and accelerate your ENPP1 program.
Lead Discovery Services Include:
Inhibitor Screening – To identify or confirm the activity with the target.
Inhibitor Potency Profiling – Dose-response with target and/or related proteins. Fast IC50 results.
Residence Time Measurements – Determination of koff using ‘jump dilution’ enzymatic assay method.
Mechanism of Action Studies – Kinetic analysis to define the mode of inhibition
CD73 Assay Services
Interested in moving your program forward, but don't want to bring an assay in-house? Our scientists can help! BellBrook scientists will use their extensive biochemistry and enzymology expertise to work with you and accelerate your CD73 program.
Lead Discovery Services Include:
Inhibitor Screening – To identify or confirm the activity with the target.
Inhibitor Potency Profiling – Dose-response with target and/or related proteins. Fast IC50 results.
Residence Time Measurements – Determination of koff using ‘jump dilution’ enzymatic assay method.
Mechanism of Action Studies – Kinetic analysis to define the mode of inhibition.
CD39 Assay Services
Interested in moving your program forward, but don't want to bring an assay in-house? Our scientists can help! BellBrook scientists will use their extensive biochemistry and enzymology expertise to work with you and accelerate your CD39 program.
Lead Discovery Services Include:
Inhibitor Screening – To identify or confirm the activity with the target.
Inhibitor Potency Profiling – Dose-response with target and/or related proteins. Fast IC50 results.
Residence Time Measurements – Determination of koff using ‘jump dilution’ enzymatic assay method.
Mechanism of Action Studies – Kinetic analysis to define the mode of inhibition.
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