BellBrook labs

Madison, Wisconsin, US

BellBrook Labs is dedicated to providing the pharmaceutical industry with enabling screening tools to accelerate the discovery of more effective therapies. As a small, R&D-driven company, we identify areas where there is a strong need but a significant technical hurdle, and then we develop a solution. BellBrook Labs is focused on taking creative approaches to new product development and to continually improving the level of service provided to our customers. When you communicate with someone from BellBrook it will be immediately apparent that we share your passion for discovering more effective disease therapies and your respect for the complexity of biology. We have included names of BBL employees and direct phone numbers on this website. Please feel free to contact any of us with questions or suggestions regarding our products and technologies or how we might better serve your needs.

*Transcreener® Biochemical... Show more »

Transcreener® Biochemical Assay Technology

BellBrook’s proprietary Transcreener® HTS Platform was developed to move more new targets into HTS more rapidly and to streamline selectivity profiling within a target family. It relies on homogeneous fluorescent immunodetection of nucleotides, including ADP, UDP, GDP, AMP and GMP, that are formed by thousands of cellular enzymes. Among these are group transferase families, such as kinases and glycosyltransferases, that catalyze the covalent regulatory reactions that are central to cell signaling. Each Transcreener Assay provides a generic detection method for multiple enzyme families. This means that you can focus on evaluating new therapeutic strategies and optimizing lead molecules rather than on time-consuming assay development for individual targets. Since the introduction of the first Transcreener assays in 2005, the platform has been rapidly validated and adopted by pharmaceutical and biotechnology screening labs around the world.

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Custom Biochemical Assay Development

Price on request

Whether you want to screen 2M compounds or perform SAR on 20, you need a robust, reliable biochemical assay that has been optimized for your target.

Leveraging BellBrook’s assay development expertise will save you time and money. Your project will be handled by the same BellBrook scientists who developed the Transcreener assays... Show more »

Whether you want to screen 2M compounds or perform SAR on 20, you need a robust, reliable biochemical assay that has been optimized for your target.

Leveraging BellBrook’s assay development expertise will save you time and money. Your project will be handled by the same BellBrook scientists who developed the Transcreener assays and have optimized them for more than 100 different enzyme targets, including kinases, ATPase, GTPases, glycosyltransferases, ligases/synthetases, and phosphodiesterases. We will define the exact conditions needed for initial velocity detection of your enzyme with outstanding signal:noise; Z’ values of 0.7 to 0.9 are typical. We will tailor our efforts to meet your specific needs, for example minimizing consumption of limited reagents or enabling simultaneous screening for inhibitors and activators. And we will work fast. If your target enzyme is in good shape, we can generally complete the assay development within two to four weeks.

Assay Development Services Include:

Target Protein Sourcing/Production – If the target protein is available from the customer or a vendor, we will test it for purity and functional activity; if not, we will produce it using the most efficient and cost effective expression and purification strategy.
Assay Optimization with Target Protein – We will determine the optimal buffer composition, enzyme concentration, and reaction time and optimize detection reagents to produce the maximal signal under initial velocity conditions
Assay Validation – Depending on customer needs, validation could include dose responses with control inhibitors, determination of kinetic parameters, pilot screens with targeted libraries, or interference screens.

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Transcreener HTS

kinase

protein kinase

lipid kinase

carbohydrate kinase

ATPase

Methyltransferase

Acetyltransferase

GTPase

GAPs

GEFs

Phosphodiesterase

Helicase

Ligase

Synthetase

Glycosyltransferase

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BellBrook labs