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Beijing SBS Genetech Co.,Ltd.

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Beijing, CN

About Beijing SBS Genetech Co.,Ltd.

Beijing SBS Genetech Co.,Ltd. is one of the earliest Chinese companies providing custom oligonucleotides synthesis, gene synthesis and custom peptide synthesis. Since we founded in 2000, we have been committed to use technology to create a future that we are a proud of. Over decades, by providing safer, higher quality and more affordable products, we have served researchers in more than 40 countries, empowering them to create new fundamental knowledge in the field of biology.


Today we not only provide custom oligos, gene synthesis and peptide synthesis services, we also offer the following products: DNA synthesis products, nucleic acids isolation & purification kits, DNA molecular weight markers, PCR-Related Products, RNA-Related Products, gene manipulation products, etc. With its remarkable characteristics, we have acquired a leading position in such fields, and contributed to life science development together with wonderful researchers. Thousands of papers have been published in the world's top academic journals with our products.


From genome sequencing of E. coli to human beings, from basic molecular cloning technique to CRISPR-Cas-mediated genome editing, from earliest combinatorial synthesis of genetic networks to chemical synthesis of two S. cerevisiae chromosome arms, we have been witnessing the miracle created by life science throughout past decades. We are proud that we realized our dream and we believe the future will be brighter with our joint efforts.

Our Services (16)


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Molecular Cloning

Price on request

Gene manipulation (molecular cloning) is a process that uses biotechnology to manipulate genes directly to generate new DNA, and has been widely used in research, medicine, industrial biotechnology and agriculture. Our Premium™ efficient seamless cloning kit, Muta-direct™ site-directed mutation kit and topo cloning kits provide efficient solutions for these types of demands.


Products


Muta-direct™ Site-Directed Mutagenesis Kit

Muta-direct™ Site-Directed Mutagenesis Kit can induce mutagenesis at the specific point of sequence that cloned on plasmid DNA. It guarantees 100% of efficiency in theory. Also it is very convenient and simple because it takes just two steps for all experimental procedures. The Site-Directed Mutagenesis Kit does not necessary using M13 vector and methylation step. Indeed, the Kit can induce mutation of nucleotide, re-mutation to wild type, mutation of codon and insertion even deletion. As the Kit has these characteristics, it is applicable to analysis for genomic/proteomic function. Also as inducing mutagenesis of specific gene, it can be used for protein engineering like protein development or improving productivity.


Premium™ Master Assembly Mix

Premium™ Master Assembly Mix is a ready-to-use reaction mix for seamless cloning reaction. It can simultaneously insert one or more PCR fragments composed of any sequence into any linearized vector in approximately 15 min with high efficiency and is not limited by the restriction sites of either the vector or the target fragment. Premium™ Master Assembly Mix is ideal for the construction of long DNA fragments and can be used in point mutation, construction of a mutation library, and other molecular cloning experiments.


pBM16K Topo Cloning Kit

pBM16K Topo Cloning Kit is not only suitable for cloning blunt-end PCR products which are amplified by high-fidelity DNA polymerases such as Pfu, sPfu, KOD, Xerox, Phusion, Q5, and SuperGold™, but also can be used to clone PCR products with an extra "A" nucleotide at the 3'end which are amplified by DNA polymerases such as Taq, Taq plus, Tth and klenTaq. The pBM16K vector in the kit is linearized. Positive clones can be identified by colony PCR with primers M13F and M13R.


pBM16A Topo Cloning Kit

pBM16A Topo Cloning Kit is not only suitable for cloning blunt-end PCR products which are amplified by high-fidelity DNA polymerases such as Pfu, sPfu, KOD, Xerox, Phusion, Q5, and SuperGold™, but also can be used to clone PCR products with an extra "A" nucleotide at the 3'end which are amplified by DNA polymerases such as Taq, Taq plus, Tth and klenTaq. The pBM16A vector in the kit is linearized. Positive clones can be identified by colony PCR with primers M13F and M13R.


pBM23 Topo Cloning Kit

pBM23 Topo Cloning Kit is not only suitable for cloning blunt-end PCR products which are amplified by high-fidelity DNA polymerases such as Pfu, sPfu, KOD, Xerox, Phusion, Q5, and SuperGold™, but also can be used to clone PCR products with an extra "A" nucleotide at the 3'end which are amplified by DNA polymerases such as Taq, Taq plus, Tth and klenTaq. The pBM16K vector in the kit is linearized. Positive clones can be identified by colony PCR with primers M13F (-47) and M13R (-48).


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PCR

Price on request

Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification.

Products

dNTPs Set (100mM each)

dNTPs Set contains 4×1ml of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of 100mM each in sterile deionized water at pH7.5, whose purity is up to 99.5% (HPLC). It is free of RNase and DNase, and suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis and nick translation.

dNTPs Mix (10mM each)

10 mM dNTPs Mix is a ready-to-use solution of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of10 mM each in sterile deionized water at pH7.5, whose purity is ≥99% (HPLC). It is free of RNase and DNase, and issuitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNAsynthesis and nick translation.

Bst DNA Polymerase

Bst DNA Polymerase was derived from Bacillus stearothermophilus DNA polymerase I. Its 5 '- 3' exonuclease activity was removed by genetic engineering, while the 5 '- 3' polymerase activity was retained. The enzyme has strong strand displacement ability, so it is an excellent enzyme for isothermal amplification. Compared with wild-type Bst DNA polymerase (large fragment), the enzyme has been greatly improved in terms of amplification speed, yield, salt tolerance and thermal stability.

Bst DNA/RNA Polymerase

Bst DNA/RNA Polymerase is a mixture of Bst DNA polymerase and extremely thermostable reverse transcriptase (65℃ tolerant), which is suitable for isothermal amplification reaction of RNA. It can detect low-sensitivity RNA molecules. This enzyme is recommended in isothermal amplification experiments using RNA as a template. In addition, Bst DNA / RNA Polymerase can also perform isothermal amplification of DNA templates .

PCR Plates

Our PCR plates are consist of 96 PCR tubes. They are compatible with most commercial PCR instruments and real-time PCR instruments, and guaranteed free of human DNA, PCR inhibitors, RNase and DNase. There plates are made from high quality polypropylene to ensure the minimal loss of reaction solution. Ultra-thin well wall design facilitates rapid and steady heat transfer. These polypropylene plates are autoclave-safe (121℃, 20 min). Semi-skirted plates are available with bar code on request.

PCR Tubes

Our PCR tubes are compatible with most commercial PCR instruments and real-time PCR instruments. These polypropylene tubes are guaranteed free of human DNA, PCR inhibitors, RNase and DNase. Ultra-thin well wall design facilitates rapid and steady heat transfer. These tubes and caps have a design that allows the caps to seal securely with the least possible pressure. With high sealing performance, the loss of reaction volume is generally less than 5%. These polypropylene tubes are autoclave-safe.


6 × TriDye DNA/RNA Loading Buffer

6 × TriDye DNA/RNA Loading Buffer can be used for checking the integrity of RNA or DNA during agarose gel electrophoresis.

PrimeTaq™ Probe One-Step RT-qPCR Kit

PrimeTaq™ Probe One-Step RT-qPCR Kit provides rapid real-time quantification of RNA targets. The kit contains optimized components that allow both reverse transcription and PCR amplification to take place. With this kit, all steps can be performed in a single tube. PrimeTaq™ Probe One-Step RT-qPCR Kit is intended for molecular biology applications.

PrimeTaq™ SYBR Green One-Step RT-qPCR Kit

PrimeTaq™ SYBR Green One-Step RT-qPCR Kit is a high-quality premix based on SYBR Green for one-step Quantitative reverse transcription PCR (RT-qPCR), which is mainly used for specific ultra-high sensitivity quantitative detection of RNA.

Taq PCR EasyMasterMix

Taq PCR EasyMasterMix is our basic solution for PCR reactions. This mix contains Taq DNA polymerase, dNTPs, MgCl2, and buffer. Taq DNA polymerase has both 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity, without 3’-5’ exonuclease activity. The extension rate of Taq DNA polymerase is 1-2 kb/min.

Faster™ PCR EasyMasterMix 

Faster™ PCR EasyMasterMix is our solution for PCR reactions which require high extension rate. This mix contains Faster™ DNA polymerase, dNTPs, MgCl2, and buffer. Faster™ DNA polymerase has both 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity, without 3’-5’ exonuclease activity. The extension rate of Faster™ DNA polymerase can reach as fast as 10 sec/kb, which is six times as high as Taq DNA polymerase. 

SuperGold™ High Fidelity PCR EasyMasterMix 

SuperGold™ High Fidelity PCR EasyMasterMix is our ultimate solution for PCR reactions which require both high fidelity (69 times as high as Taq DNA polymerase) and high extension rate (4 times as high as Taq DNA polymerase). This mix contains SuperGold™ High Fidelity DNA polymerase, dNTPs, MgCl2, and buffer. Among them, SuperGold™ High Fidelity DNA polymerase has extremely high fidelity, which is 69 times as high as Taq DNA polymerase. Extremely high fidelity makes it possible to clone ultra-long fragments. And simple templates such as plasmids and lambda DNA can be amplified by SuperGold™ High Fidelity PCR MasterMix with an effective length of 40 kb, genomes can be amplified with an effective length of 20 kb, and difficult fragments such as cDNA can be amplified with an effective length of 10 kb. In addition, the extension rate of SuperGold™ High Fidelity DNA polymerase can reach as fast as 15 sec/kb, which is four times as high as Taq DNA polymerase. 

2 x Taq PCR MasterMix

The 2 x Taq PCR MasterMix is an optimized ready-to-use PCR mixture of Taq DNA Polymerase, PCR buffer, MgCl2, dNTPs, and PCR dye. The 2 x Taq PCR MasterMix contains all components for PCR, except DNA template and primers. The Taq DNA has no 3’→5’exonuclease activity, so it is suitable to amplification of DNA fragment less than 5Kb, and the PCR product has an “A” base at the 3’terminal, thus it is very convenient to clone to T- vector. After PCR, samples can be removed from the reaction and loaded directly onto agarose gel. The green dye, acting as a tracking dye, migrates at about the same rate as 3-5kb DNA fragment in 1% agarose gel.   

f-Pfu DNA Polymerase

f-Pfu DNA Polymerase is a thermostable enzyme with a molecular weight of 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction, resulting in blunt-ended PCR products without 3'-dA overhangs. f-Pfu DNA Polymerase exhibits 3'→5' exonuclease (proofreading) activity that enables the polymerase to correct nucleotide-misincorporation errors, and lacks 5'→3' exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter. 

sPfu DNA Polymerase

sPfu (super Pfu) DNA polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3’ direction, resulting in blunt-ended PCR products without 3’-dA overhangs. sPfu DNA Polymerase exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct mis-incorporated nucleotides, and lacks 5’→3’ exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter. The fidelity is 64 times higher than Taq DNA Polymerase, and 8 times higher than regular Pfu DNA Polymerase. 

U-Taq DNA Polymerase

U-Taq DNA Polymerase consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5'→3' DNA polymerase activity and lacks 3'→5' exonuclease activity. Its extension rate is 2~4 kb/min in standard condition, which is the highest among all thermostable DNA enzyme. The appropriate reaction temperature is 70~75℃, the work concentration of dNTPs is 100~300 μM, the work concentration of Mg2+ is 2~3 mM, and the suitable pH is 8.1~9.1. The enzyme generates PCR products with 3'-dA overhangs, suitable for T-A cloning. The amount of enzyme is 1~1.5unit for 20μl PCR reaction, while 2~3unit for 50μl PCR reaction. 6 kb Lambda DNA and 2.1 kb Human genomic DNA can be amplified very well at our laboratory.


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DNA Purification

Price on request

DNA purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our DNA purification kits use both first-class silica gel column technology and magnetic beads technology, which can purify DNA from various sources quickly and reliably. The purity of DNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning and cleavage.




Products




VirusMag™ Magnetic Beads


VirusMag™ Magnetic Beads are composed of silica with coated superparamagnetic iron oxide particles. The beads are designed for efficient nucleic acid (DNA/RNA) isolation and purification for a range of samples, from biofluids to liquid biopsy. The beads provide an optimal content of ferric oxide for high magnetization in the presence of a magnetic field and low sedimentation rate. At the same time, they provide good binding capacity. The surface functional group is -OH.




VSep™ Magnetic Separators


VSep™ magnetic separators are durable and easy-to-use. The separators consist of a series of powerful NdFeB (neodymium-iron-boron) magnetic disks. Each magnetic disk attracts the beads in tubes fitted in an adjacent hole. VSep™ magnetic separators are widely used in cell sorting, RNA and DNA isolation and purification of biomolecules.




VirusMag™ DNA/RNA Isolation Kit


VirusMag™ DNA/RNA Isolation Kit is a research use only (RUO) kit, which is based on biological nanomagnetic beads. The main principle is to enrich nucleic acid from sample lysate products to the surface of magnetic beads by using functional groups on the surface of functional biological magnetic beads, and then separate the magnetic beads by using a magnetic separation device, so as to quickly separate and purify nucleic acid.




VirusMag™ One-Step DNA/RNA Isolation Kit


VirusMag™ One-Step DNA/RNA Isolation Kit is a research use only (RUO) kit, which is designed for the isolation of viral RNA/DNA or bacterial DNA from cell-free body fluids such as serum, plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab by using magnetic beads and unique buffer system.




SiMax™ Spin Column


SiMax™ Spin Column is miniprep spin column, which can be used for plasmid, PCR products, genomic DNA isolation, as well as agarose gel purification. In a high-salt buffer, DNA is selectively bound to the membrane in SiMax™ Spin Column. Following a wash step, DNA is eluted in low-salt buffer or water without alcohol precipitation or desalting.




SiMax™ Genomic DNA Extraction


The SiMax™ Genomic DNA Extraction Kit is designed for rapid, small scale isolation of genomic DNA from a wide range of samples. In a high-salt buffer, DNA is selectively bound to the SiMax™ membrane in a spin column. Following a wash step, DNA is eluted in low-salt buffer or water without alcohol precipitation or desalting. Although the protocol provided in this kit is for the isolation of genomic DNA from whole blood, the kit can also be used for extracting DNA from many other samples.




SiMax™ PCR Products/Agarose Gel Purification


The SiMax™ PCR Products/Agarose Gel Purification Kit is designed for the rapid purification of PCR products, or for the efficient extraction of DNA fragments from agarose gel. In a high-salt buffer, DNA is bound to the SiMax™ membrane in a spin column. Following a wash step, DNA is eluted in low-salt buffer or water without alcohol precipitation or desalting. This kit removes DNA polymerase, dNTPs, and salts. DNA fragments of 50 bp to 20 kb can be cleaned up within 20 minutes.




SiMax™ Plasmid DNA Miniprep


The SiMax™ Plasmid DNA Miniprep Kit is designed for the rapid purification of plasmid DNA. In a high-salt buffer, DNA is bound to the SiMax™membrane in a spin column. Following a wash step, the DNA is eluted in low-salt buffer or water without alcohol precipitation or desalting. 5~15 μg of a high-copy number plasmid DNA can be isolated from 3 ml of bacterial culture in 30 minutes. This kit removes proteins, RNA, and low molecular weight impurities. The high-quality plasmid DNA can be used for restriction enzyme digestion, PCR, automated sequencing, manual sequencing, and transformation.




Funcbeads™ Plasmid Purification Kit


The unique modified functional groups on the surface of magnetic beads have strong specific adsorption on plasmid DNA under specific conditions. When the surrounding environmental conditions are changed, DNA can be detached from the surface of magnetic beads, thus achieving the purpose of rapid separation and purification of DNA. Proteins and other impurities can be removed to the maximum extent, thus ensuring the purity of the extracted plasmid. Plasmid DNA extracted by this kit can be used in various molecular biology experiments, such as enzymatic digestion, sequencing, library screening, ligation, transformation, etc.




Funcbeads™ PCR Gel Purification Kit


Funcbeads™ PCR Gel Purification Kit utilizes the characteristics of DNA binding and detaching from magnetic beads under specific conditions, which can effectively recover DNA fragments from TAE or TBE agarose gels. The kit can also effectively remove impurities such as agarose, primer dimer, dNTP, other organic compounds, inorganic salt ions and proteins.




Funcbeads™ PCR Clean Up Kit


Funcbeads™ PCR Clean Up Kit utilizes the characteristics of DNA binding and detaching from magnetic beads under specific conditions, which can effectively purify PCR products over 150 bp. The kit can also effectively remove impurities such as primer dimer, dNTP, inorganic salt and protein. Funcbeads™ PCR Clean Up Kit has several advantages such as high recovery, simplicity and rapidity.




Funcbeads™ Modified Magnetic Beads


Funcbeads™ Modified Magnetic Beads cover 3 different types of magnetic beads, including Funcbeads™ Amino Magnetic Beads, Funcbeads™ SA (streptavidin) Magnetic Beads, and Funcbeads™ Carboxyl magnetic beads. Each type of Funcbeads™ Modified magnetic beads utilizes different reaction principle to bind other molecules and has specific applications.




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Lab Consumables

Price on request

We provide various lab consumables with high cost-effective ratio to make your experiments smoother!




Products




SiMax™ Spin Column


SiMax™ Spin Column is miniprep spin column, which can be used for plasmid, PCR products, genomic DNA isolation, as well as agarose gel purification. In a high-salt buffer, DNA is selectively bound to the membrane in SiMax™ Spin Column. Following a wash step, DNA is eluted in low-salt buffer or water without alcohol precipitation or desalting.




Conical Cryovials 


We have conical cryovials in different sizes (1.5 ml and 2 ml) with various color films on top.




96-well Deep-Well Plate


96-well Deep-Well Plate has uniform plate thickness and uniform pore size. Free DNA within the wells can be extracted. It is suitable for storage of polar organic solutions, including acidic and alkaline solutions.




Serological Pipettes


Our serological pipettes cover six different volumes. The pipettes are constructed from polystyrene of high clarity for sterile serological and tissue culture applications. Color-coding on the pipettes provides easy identification. The black printed, easy to read ascending and descending graduations simplify fluid measuring and dispensing. All pipettes feature negative graduations for extra working volume. These polystyrene serological pipettes are perfect for use with all popular pipette controllers.




Low-Retention Pipette Tips, Filtered, Sterile


Here, a unique molding process is employed to produce our Low-Retention Pipette Tips. The surfaces of our Low-Retention Pipette Tips are produced through a highly special physiochemical process, which dramatically reducing retained sample after dispensing. Meanwhile, with filter inside tips, cross contamination can be largely prevented. We also offer Pipette Tips with other specifications.




PCR Tubes


Our PCR tubes are compatible with most commercial PCR instruments and real-time PCR instruments. These polypropylene tubes are guaranteed free of human DNA, PCR inhibitors, RNase and DNase. Ultra-thin well wall design facilitates rapid and steady heat transfer. These tubes and caps have a design that allows the caps to seal securely with the least possible pressure. With high sealing performance, the loss of reaction volume is generally less than 5%. These polypropylene tubes are autoclave-safe.




PCR Plates


Our PCR plates are consist of 96 PCR tubes. They are compatible with most commercial PCR instruments and real-time PCR instruments, and guaranteed free of human DNA, PCR inhibitors, RNase and DNase. There plates are made from high quality polypropylene to ensure the minimal loss of reaction solution. Ultra-thin well wall design facilitates rapid and steady heat transfer. These polypropylene plates are autoclave-safe (121℃, 20 min). Semi-skirted plates are available with bar code on request.




Centrifuge Tubes


Our centrifuge tubes are used to contain liquids during centrifugation, which separates the sample into its components by rapidly rotating it around a fixed axis. With conical bottoms, any solid or heavier parts of the sample being centrifuged can be collected. Our centrifuge tubes have large white writing areas and clear black graduations, and are guaranteed free of human DNA, PCR inhibitors, RNase and DNase. They can be safely used up to 12500 rcf, with temperature range from -80℃ to 120℃.




Centrifuge Bottles


Our centrifuge bottles are ideal for low to moderate speed centrifugation of large volumn biological and chemical samples. Made of high quality polypropylene, they have excellent chemical resistance, and translucent walls provide visibility of contents. Our centrifuge bottles are reusable and autoclavable, and guaranteed free of human DNA, PCR inhibitors, RNase and DNase. With leakage proof cap, samples are protected from cross contamination.




Microcentrifuge Tube


Our microcentrifuge tubes feature easy-to-read graduations and smooth inner walls for easy filling and sample preparation. Crystal-clear polymer facilitates visualization of samples. Our tubes are guaranteed non-pyrogenic, DNase/RNase free, and with endotoxin less than 0.1 EU. They can be safely used up to 30000xg rcf, with temperature range from -80℃ to 120℃. These polypropylene tubes are autoclave-safe.




Cell Culture Plates


Our Cell Culture Plates are available in 6-. 12-, 24-, 48- and 96 well formats. They are all made of high clarity, 100% virgin polystyrene, which facilitates visualization of cultured cells. With vacuum plasma treatment, even cell attachment can be achieved. These polystyrene plates are guaranteed non-pyrogenic and DNase/RNase free.




Cell Culture Dishes


Our Cell Culture Dishes are available in 35 mm, 60 mm, and 100 mm diameters. They are all made of high clarity, 100% virgin polystyrene, which facilitates visualization of cultured cells. Ultra flat optical clear bottom further ensures easy observation. These polystyrene dishes are guaranteed non-pyrogenic, DNase/RNase free, and TC-treated, which provides clean, uniform and compatible surfaces for cell attachment and growth.




ELISA Plates


Our ELISA Plates are specifically designed for ELISA (enzyme-linked immunosorbent assay). These plates all made of polystyrene, which facilitates visualization. The overall sizes of plates are in accordance with the ANSI-SBS standard. We offer these polystyrene in different specifictions: medium and high binding as well as detachable and non-detachable.


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DNA Ladders

Price on request

DNA Ladders are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.




Products




50bp Ladder (for PAGE)


50 bp Ladder (for PAGE) consists of nine DNA fragments ranging from 100 to 700 bp and is supplied in loading buffer containing tracking dye and precipitant for PAGE gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp, 150 bp, 200 bp, 250 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700bp.




DNA MarkerⅠ (for PAGE)


DNA Marker I (for PAGE) consists of seven DNA fragments ranging from 100 to 700 bp and is supplied in loading buffer containing tracking dye and precipitant for PAGE gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100bp, 200bp, 300bp, 400bp, 500bp, 600 bp, 700bp.




λDNA /HindⅢ Marker


λDNA /HindⅢ Marker consists of eight DNA fragments ranging from 125 to 23130 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is 23130bp, 9416bp, 6557bp, 4361bp, 2322bp, 2027bp, 564bp, and 125bp.




1kb Ladder Plus DNA Marker


1kb Ladder Plus DNA Marker consists of twelve DNA fragments ranging from 100 to 10000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100bp(50ng/5μl), 250bp(50ng/5μl), 500bp(50ng/5μl), 750bp(75ng/5μl), 1000bp(50ng/5μl), 2000bp(100ng/5μl), 3000bp(50ng/5μl), 4000bp(100ng/5μl), 5000bp(50ng/5μl), 6000bp(50ng/5μl), 8000bp(50ng/5μl), 10000bp(50ng/5μl).




1kb Ladder DNA Marker


1kb Ladder DNA Marker consists of eight DNA fragments ranging from 1000 to 10000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:1000bp(50ng/5μl), 2000bp(50ng/5μl), 3000bp(50ng/5μl), 4000bp(100ng/5μl), 5000bp(50ng/5μl), 6000bp(50ng/5μl), 8000bp(50ng/5μl), 10000bp(50ng/5μl).




100bp Ladder Plus DNA Marker


100bp Ladder Plus DNA Marker consists of fourteen DNA fragments ranging from 100 to 5000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 200 bp (30ng/5μl), 300 bp (30ng/5μl), 400 bp (40ng/5μl), 500 bp (50ng/5μl), 600 bp (30ng/5μl), 700 bp (35ng/5μl), 800 bp (40ng/5μl), 900 bp (45ng/5μl), 1000 bp (50ng/5μl), 1500 bp (75ng/5μl), 2000 bp (50ng/5μl), 3000 bp (30ng/5μl), 5000 bp (50ng/5μl).




100bp Ladder DNA Marker


100bp Ladder DNA Marker consists of eleven DNA fragments ranging from 100 to 1500 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 200 bp (30ng/5μl), 300 bp (30ng/5μl), 400 bp (40ng/5μl), 500 bp (50ng/5μl), 600 bp (30ng/5μl), 700 bp (35ng/5μl), 800 bp (40ng/5μl), 900 bp (45ng/5μl), 1000 bp (50ng/5μl), 1500 bp (75ng/5μl).




50bp Ladder DNA Marker


50bp Ladder DNA Marker consists of ten DNA fragments ranging from 50 to 700 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:50bp(50ng/5μl), 100bp(50ng/5μl), 150bp(30ng/5μl), 200bp(30ng/5μl), 250bp(25ng/5μl), 300bp(30ng/5μl), 400bp(40ng/5μl), 500bp(25ng/5μl), 600bp(30ng/5μl), 700bp(35ng/5μl).




DNA MarkerⅣ


DNA Marker IV consists of seven DNA fragments ranging from 200 to 5000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:200 bp (50ng/5μl), 500 bp (50ng/5μl), 800 bp (40ng/5μl), 1200 bp (60ng/5μl), 2000 bp (50ng/5μl), 3000 bp (30ng/5μl), 5000 bp (50ng/5μl).




DNA MarkerⅢ


DNA Marker III consists of six DNA fragments ranging from 200 to 1500 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:200 bp (50ng/5μl), 400 bp (40ng/5μl), 600 bp (30ng/5μl), 800 bp (40ng/5μl), 1000 bp (50ng/5μl), 1500 bp (75ng/5μl).




DNA MarkerⅡ


DNA Marker II consists of six DNA fragments ranging from 100 to 1200 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 300 bp (30ng/5μl), 500 bp (50ng/5μl), 700 bp (35ng/5μl), 900 bp (45ng/5μl), 1200 bp (60ng/5μl).




DNA MarkerⅠ


DNA Marker I consists of seven DNA fragments ranging from 100 to 700 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 200 bp (30ng/5μl), 300 bp (30ng/5μl), 400 bp (40ng/5μl), 500 bp (25ng/5μl), 600 bp (30ng/5μl), 700 bp (35ng/5μl).




BM15000 DNA Marker


BM15000 consists of seven DNA fragments ranging from 250 to 15000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:250 bp (50ng/5μl), 1000 bp (50ng/5μl), 2500 bp (50ng/5μl), 5000 bp (50ng/5μl), 7500 bp (40ng/5μl), 10000 bp (40ng/5μl), 15000 bp (40ng/5μl).




BM8000 DNA Marker


BM8000 consists of nine DNA fragments ranging from 100 to 8000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 250bp (50ng/5μl), 500bp (50ng/5μl), 750bp (75ng/5μ), 1000bp (50ng/5μl), 2000bp (100ng/5μl), 3000bp (30ng/5μl), 5000bp (50ng/5μl), 8000bp (50ng/5μl).




BM5000+ DNA Marker


BM5000+ consists of nine DNA fragments ranging from 100 to 5000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 250 bp (50ng/5μl), 500 bp (50ng/5μl), 750 bp (75ng/5μ), 1000 bp (50ng/5μl), 1500 bp (75ng/5μl), 2000 bp (100ng/5μl), 3000 bp (30ng/5μl), 5000 bp (50ng/5μl).




BM5000 DNA Marker


BM5000 consists of eight DNA fragments ranging from 100 to 5000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 250 bp (50ng/5μl) , 500 bp (50ng/5μl), 750 bp (75ng/5μl), 1000 bp (50ng/5μl), 2000 bp (100ng/5μl), 3000 bp (30ng/5μl), 5000 bp (50ng/5μl).




BM2000+DNAMarker


BM2000+ consists of seven DNA fragments ranging from 100 to 2000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 250 bp (50ng/5μl), 500 bp (50ng/5μl), 750 bp (75ng/5μl), 1000 bp (50ng/5μl), 1500 bp (75ng/5μl), 2000 bp (100ng/5μl).




BM2000 DNA Marker


BM2000 consists of six DNA fragments ranging from 100 to 2000 bp and is supplied in loading buffer containing tracking dye and precipitant for agarose gel electrophoresis, which is in a convenient ready-to-load format. The recommended volume for each lane is 5ul, and the amount of each band is:100 bp (50ng/5μl), 250 bp (50ng/5μl), 500 bp (50ng/5μl), 750 bp (75ng/5μl), 1000 bp (50ng/5μl), 2000 bp (100ng/5μl).




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Biochemical Reagents

Price on request

Biochemistry, as its name implies, is a discipline that studies chemical processes in organisms, often referred to as biochemistry. It is mainly used to study the structure and function of various intracellular components, such as proteins, sugars, lipids, nucleic acids and other biomacromolecules. We provide variety types of biochemical reagents to meet these demands.




Products




Proteinase K


Our Proteinase K is a non-specific serine protease that has been purified to remove RNase and DNase activities. It is active in the presence of SDS or urea and in a wide range of pH, salt concentration and temperature. Its activity will increase in 1% SDS. Further discount will be offered for bulk order.




Mutant Proteinase K


The application of this Mutant Proteinase K is similar with wild type Proteinase K. But this mutant one has higher specific activity and more stable at room temperature. It is a non-specific serine proteinase with broad substrates. It is active over the pH range from 4 to 12. It can be used at any situation to digest native and denatured proteins. For instance, it is used for isolating mRNA or genomic DNA from different tissues and modifying glycoprotein for structure studies. Mutant Proteinase K is active with SDS, urea and EDTA and active between 15°C and 75°C.




Bovine Serum Albumin


Bovine serum albumin (BSA) is a serum albumin protein derived from cows. In molecular biology lab experiments, BSA is widely used for stabilizing restriction enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipette tips, and other vessels. Here we offer 3 different specifications: BSA Fraction V, BSA Protease Free, and BSA Fatty Acid Free.




PreScission Protease (PSP)


PreScission Protease is a genetically engineered fusion protein of human rhinovirus 3C protease and glutothione S trasferase (GST). PreScisson protease specifically cleaves between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.




TEV Protease


TEV protease has (NIa) protease catalytic domain which corresponds to a molecular weight of 27 kDa. It is unique with high specificity and is active at low temperature. The tobacco etch virus (TEV) protease is a useful tool for the removal of fusion tags from recombinant proteins.




SUMO Protease


SUMO proteases are a general class of enzymes that specifically remove the post-translational protein modification (PTM) known as small ubiquitin-related modifier (SUMO), which falls into the PTM class of ubiquitin and/or ubiquitin-like proteins (UBL). The enzyme commonly referred to as ‘SUMO protease’ is the Ubl-specific protease 1 (Ulp1) from Saccharomyces cerevisiae. This was the first of this class of enzymes to be isolated.




Enterokinase


Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.




DNase I (RNase Free)


DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and even chromatin.




5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) 


C14H15BrCINO6, MW 408.64, purity>99%. X-Gal, a histochemical substrate for β-galactosidase, fields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.




Isopropyl-beta-D-thiogalactopyranoside (IPTG) 


C9H18O5S, MW 238.31, purity>99%.




Besta™ LE Agarose


Besta™ LE Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances ease of gel processing and handling.


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DNA Synthesis and Probe Development

Price on request

Since the establishment, we have been unremittingly providing high-quality DNA Synthesis Products and Molecular Beacons for customers around the world, and the products have been successfully applied in various fields of molecular biology. 




DNA Synthesis Products




For DNA synthesis products , we are offering:


Synthesis Columns for ABI 3900, MerMade, Dr. Oligos, OligoMaker, ASM-2000, etc.

Universal Support-CPG

Standard Support-CPG

DNA Phosphoramidites

LNA Phosphoramidites

Oligonucleotide Purification Resin

Modified Amidites & CPG







Molecular Beacons




Molecular Beacons have a fluorescent reporter and a quencher at their 5' and 3' ends, respectively. The sequence of these probes is designed so that they form a hairpin structure in which the fluorescent dye and the quencher are in close proximity. A Molecular Beacon specific for the sequence of interest is used in PCR. The probe is designed to anneal between the PCR primers. When the probe hybridizes to its target sequence in the PCR annealing step, the loop opens and the fluorescent reporter and quencher are separated, resulting in a fluorescent signal. The amount of signal is proportional to the amount of target sequence, and is measured in real time to allow quantification of the amount of target sequence.




Beijing SBS Genetech is a licensed supplier of Molecular Beacons, and offers a large number of fluorescent reporters and different quenchers. Molecular Beacons with a dabcyl quencher and a variety of different fluorescent reporters can be conveniently ordered from Beijing SBS Genetech. All Molecular Beacons provided are PAGE purified.


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Peptide Libraries

Price on request

A peptide library is a new technique for studying structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.




We supply peptide library as either desalt or >70%, >80%, >90%, and >95% purities. Peptides can be made in required amounts from minimal to mg scale for initial screening. Appropriate peptide labels (Biotin, FITC, etc.) can be added upon request. Each peptide in the library undergoes rigorous quality control to avoid any cross contaminants before delivery. All purified peptides are delivered with complete QC data including RP-HPLC, MS, and COA report to ensure high quality. The desalted peptides are provided with MS only. Peptide length in a given library is usually 5-15 aa with 1-5 amino acid overlap.




Peptide Library Types


Depending upon a given application, peptide libraries are usually defined as:


Overlapping Peptide Library

Alanine Scanning Library

Truncation Library

Random/scrambled Library

Positional Scanning Library


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siRNA Synthesis

Price on request

We are proud to offer custom miRNA and siRNA with our professional synthesis and purification technology.




Features


We can provide as short as 2 nt DNA/RNA synthesis services to as long as 200 nt DNA, 70 nt RNA synthesis services.

We can provide various DNA/RNA modifications.

We provide both mass spectrometry and Waters high performance liquid chromatography analysis. You can use our custom miRNA with confidence.

We can provide synthesis services from microgram to hundreds gram.

Turnaround time is short. For routine modifications, only 2-3 working days are needed for the synthesis and purification.

The purity of clinical test grade is more than 99%, with high stability and no cross-contamination.

Storage


DNA: The DNA dry powder is stable and can be stored at -20 C for 2 years. Dissolution of DNA can be done with sterile water or TE, and the dissolved DNA is best stored at -20 C. Repeated freeze-thaw should be avoided.

RNA: Because the stability of RNA is much worse than that of DNA, it is recommended that the RNA be stored at -80 C in either dry powder or solution state, and the RNA in solution state should be avoided from repeated freezing and thawing. Modified oligonucleotides are recommended to be stored up to -20 C, and fluorescent modifications need to be kept away from light.



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MicroRNA (miRNA) Synthesis

Price on request

We are proud to offer custom miRNA and siRNA with our professional synthesis and purification technology.


Features


We can provide as short as 2 nt DNA/RNA synthesis services to as long as 200 nt DNA, 70 nt RNA synthesis services.

We can provide various DNA/RNA modifications.

We provide both mass spectrometry and Waters high performance liquid chromatography analysis. You can use our custom miRNA with confidence.

We can provide synthesis services from microgram to hundreds gram.

Turnaround time is short. For routine modifications, only 2-3 working days are needed for the synthesis and purification.

The purity of clinical test grade is more than 99%, with high stability and no cross-contamination.

Storage


DNA: The DNA dry powder is stable and can be stored at -20 C for 2 years. Dissolution of DNA can be done with sterile water or TE, and the dissolved DNA is best stored at -20 C. Repeated freeze-thaw should be avoided.

RNA: Because the stability of RNA is much worse than that of DNA, it is recommended that the RNA be stored at -80 C in either dry powder or solution state, and the RNA in solution state should be avoided from repeated freezing and thawing. Modified oligonucleotides are recommended to be stored up to -20 C, and fluorescent modifications need to be kept away from light.



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PNA Synthesis

Peptide Nucleic Acid Synthesis
Price on request

Description


PNA (Peptide Nucleic Acid), an artificially created DNA analogue, was first invented by Professor Nielsen, Egholm, Berg and Buchardt of the University of Copenhagen, Denmark in 1991. 


PNA has a structure in which the phosphate-ribose backbone of DNA is substituted with a peptide-like amide backbone (N-(2-aminoethyl) glycine). So the binding affinity and stability to the target DNA or RNA are greatly increased. Despite the structural change of the backbone, PNA can be used in a variety of applications where DNA can be used because it can make a complementary binding to the target sequence as DNA does.


PNA Applications

  • Sequence specific PCR blocker (PNA clamp)
  • FISH probes for telomere, centromere, gene specific probes, infection test
  • Anti-sense/ anti-microbial reagents
  • miRNA inhibitors
  • Double strand DNA invasion & capture
  • Microarray probes

 

Unlabeled PNA


Due to its high affinity and specificity, PNA oligos can efficiently bind its target nucleic acid. One of the popular usage of unlabeled PNA is a gene specific blocker of PCR reaction (PNA clamp). This technology can efficiently detect SNP mutations in a target gene.

PNA binds to its target nucleic acid by either orientation but antiparallel is preferred over parallel. 5' end of PNA is NH2- (also written as 5' or H-) that can be conjugated to other functional groups, and 3' end of PNA is -CONH2 (also written as -NH2 or 3'), which is inactive end. Acetylation at 5' end can block any potential reactivity.


Because Tm of PNA is higher than that of DNA, usually 10~21mer is used for most applications. Longer length of PNA can reduce solubility. High purine content (>60%), specially G base can be a reason for low solubility. Depending on the sequence, possible longest length of PNA is about 40 mer. However, it is strongly recommended to check its Tm and design the probe that has appropriate length and sequence.


To improve solubility of PNA probes, addition of solubility enhancer such as O linker, E linker, X linker, or 2 Lysines is recommended for PNA with long length (>22mer) or high purine content (>60%). These linkers can also work as a spacer for conjugation to other functional groups such as peptides or dyes.


Labeled PNA


PNA oligomers can be labeled at 5' and/or 3' end. Since 3' end PNA is inactive (-CONH2), one Lysine is added and its amine is used for conjugation at 3' labeling.

It is recommended to include 1~2 O linkers (also called eg1, or AEEA linker) between the label and PNA for 5' end labeling.

Possible modifications:

  • Fluorophore/Quencher:Cyanine dye;FAM;FITC;TAMRA (TMR);TexasRed;ROX;HEX;ATTO dye;Thiazol Orange;Methylene Blue;Dabcyl;BHQ;Digoxigenine;Biotin 
  • Linker:C3-NH2, C4-NH2;C6-NH2;C12-NH2;Polyethylene Glycol linker;E linker
  • Functional group:Amine;Alkyne;Thiol;Maleimi;Azide;Acetylation
  • Backbone modification:Alpha modification;Gamma modification
  • Base modification:D (2,6-diaminopurine);J (Pseudoisocytosine);I (Inosine)


PNA-peptide


Since the backbone of PNA is based on poly amides, PNA can be easily linked to peptide to add functionality. For example, Lys addition can improve solubility of PNA. Addition of Cysteine can be used as a way to conjugate other molecules using disulfide bond formation.


Peptide can be conjugated at 5' end or 3' end but 5' end conjugation (peptide+PNA) is more popular. O linker can be added between peptide and PNA as a spacer.


One of the most popular applications of PNA peptide conjugation includes antimicrocidal reagents, which comprise of CPP (most commonly (KFF)3K or (RXR)4XB and 10~15 basepair of PNA molecules that are antisense to the essential gene of the microbes.


Similarly in mammalian cells, anti-sense oligo approach can be easily adapted using CPP and PNA conjugation where PNA is designed antisense to its target mRNA. Most commonly, PNA is degisned to 5' ATG and upstream region.


Another approach using peptide and PNA conjugation is in microarray type where you can capture both antigene and transcript from the target microorganisms.


In general, PNA peptide conjugates are consecutively synthesized on resin from C-terminus to N-terminus.


Gamma PNA


Gamma (γ)-PNA is a backbone-modified PNA that possesses a stereogenic center through modifications introduced at gamma (γ)-carbon of the backbone. Due to the stereogenic center, the gamma-PNA oligo itself forms an alpha-helical structure, thereby reducing the self-aggregation, improving the solubility and forming a more stable duplex with the target DNA. These features provide higher binding affinity to the target. In addition, various modifications such as internal multi-labeling are possible at any gamma (γ)-position. With these advantages, gamma (γ)-PNA can be an attractive material for diagnostics and drug development.


Possible gamma functional groups

  • Lysine: better solubility, possible for dual labeling, potential for cell penetration
  • MiniPEG: best for improved solubility and specific binding, efficient for double strand DNA invasion
  • Alanine and glutamic acid are also possible modification.


PNA Design


Binding properties: PNAs can form duplexes in either orientation, but the anti-parallel orientation is strongly preferred. This will be the orientation for all antisense and DNA probe type applications. The N-terminal of the PNA oligomer is equivalent to the 5'-end of an oligonucleotide and is often referred to as "the 5'-end of the PNA". A PNA/DNA-duplex will usually have a higher Tm than the corresponding DNA/DNA-duplex. As a rough rule, there will be an increase in Tm of about 1°C per base pair at 100 mM NaCl depending on the sequence.


Probe Length: Due to this higher affinity it is not necessary to prepare long PNA oligomers. For most applications an oligomer length of 12-18 is optimal. as opposed to the 25-40-mers, which is the typical length for an oligouncleotide probe. Bear in mind that the shorter a probe the more specific it is. The impact of a mismatch is greater, the shorter the sequence is. In many cases even shorter probes will work well, Longer PNA oligomers, depending on the sequence, tend to aggregate and are difficult to purify and characterize.

Purine Content: Purine rich PNA oligomers tend to aggregate, with G-rich oligomers being the worst. As a rule, never have more than 7 purines in any stretch of 10 units. Observing this rule will dramatically reduce the likelihood that the PNA oligomer will aggregate. Then shorter the sequence the less attention needs to be paid to the sequence design.

Self-complementarity: Avoid self-complementary sequences such as inverse repeats, hairpin forming and palindromic sequences as PNA/PNA interactions are even stronger than PNA/DNA interactions. For example, AATT would be OK but not CCGG or ATTATT. There is no problem with the synthesis but they are generally difficult to characterize and purify.


PNA Order


The price of custom oligo is dependent on the length, amount and label. Please indicate the specifics in quote request.


Minimum amount is 20 nmole for non-labeled PNA and 10 nmole for labeled PNA.

Custom PNA oligos will be provided at >90% or >95% purity by HPLC analysis along with MALDI-TOF report.


Synthesis takes 2~3 weeks for the most cases, and 3~4 weeks for gamma PNA or special labeling.


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sgRNA Synthesis

Price on request

Description


CRISPR/Cas9 genome editing technology is powerful for genome engineering like gene knock-ins and knock-outs. Genome engineering using synthetic sgRNAs yields more efficient and consistent editing results than any other types of guide RNA.


With excellent sgRNA synthesis and purification technology, we provide high-quality synthetic sgRNAs, which can be applied in various genome engineering. Our exclusive long-chain RNA synthesis technology can support around 100nt sgRNA synthesis. Normally we would provide 2'-O-methyl 3' phosphorothioate modifications in the first and last 3 nucleotides but we can also provide other types of customized modifications.




Features


Our professional sgRNA synthesis and purification technology will make your experiment more likely to be successful.

We provide high precision mass spectrometry reports.

We can ensure single main peak and theoretical molecular weight consistent with the detected molecular weight.

The purity is greater than 90%.




Storage


We recommend that RNA should be stored at -80℃ in either dry powder or solution state. RNA in solution state should also be avoided from repeated freezing and thawing. Modified oligonucleotides are recommended to be stored at -20℃.


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Peptide Synthesis

Price on request

With more than 10 years experience in solid phase peptide synthesis, SBS Genetech is committed to supplying high quality peptides at competitive prices and providing our customers with the extensive services and products.




Features:


Purities from desalt to 98%

Phosphorylation/Fluorescein/Biotin labeled peptides

Cyclic peptides

KLH/BSA Conjugation

MAP peptides

2-3 weeks turnaround time


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Gene Synthesis

Price on request
Length Price Turnaround
Up to 300 bp $115 per gene 1-2 weeks
300 bp to 1,000 bp $0.38 per bp 1-2 weeks
1,001 bp to 2,000 bp $0.43 per bp around 2 weeks
2,001 bp to 3,000 bp $0.50 per bp 2-3 weeks
3,001 bp to 5,000 bp $0.58 per bp around 3 weeks
Larger than 5,000 bp inquiry inquiry

Assuming all sequences are codon optimized for protein expression and high GC content and repeats sequences can be modified for easy manipulation and synthesis to meet required turnaround times.

In the event the construct is toxic to E.coli or will not clone, the customer will be immediately contacted and an alternative strategy will be agreed upon to generate the desired construct (an additional fee may be added).

Delivery Specs:

2-5ug of plasmid DNA containing the synthesized gene.
A stab of E.coli harboring the plasmid and gene.
Certificate of Analysis for each construct.
Sequence trace files, double strand sequence verification.
Text and Doc files of the gene sequence alone and combined with plasmid.

Subcloning:

We can custom clone each gene into a commercially available E.coli vector at $50/each, and clone into non-commercial vector at $100/each.Sequences larger than 3kb are quoted on an individual basis. Complexity may also result in a price change.


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Oligonucleotide Probes

Price on request

Taqman probes have a fluorescent reporter and a quencher at their 5' and 3' ends, respectively. These probes can be used in quantitative PCR systems that take advantage of the 5'-3' exonulease activity of Taq DNA polymerase. A probe specific for the sequence of interest is used in PCR together with specific PCR primers. This probe is designed to anneal between the PCR primers. During the extension phase of PCR, the 5'-3' exonulease activity of Taq DNA polymerase cleaves the fluorescent reporter from the probe. The amount of free reporter accumulates as the number of PCR cycles increases. The fluorescent signal from the free reporter is measured in real time and allows quantification of the amount of target sequence.




Beijing SBS Genetech offers a large number of fluorescent reporters and different quenchers for Taqman probes. Taqman probes with Black Hole Quenchers or a TAMRA quencher and a variety of different fluorescent reporters can be conveniently ordered from Beijing SBS Genetech. All Taqman probes provided are PAGE purified.


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Oligonucleotide Synthesis

Price on request

Our oligos are synthesized with a typical coupling efficiency of 99% and purified by QPC free of charge. PAGE purification is also available with additional charge. The high coupling efficiency of our synthesis technology allows efficient synthesis of oligos up to 120 bases. In addition, a variety of modifications are available. Every oligo is analyzed by PAGE to check oligo quality. Malidi-Tof Mass QC is also employed to examine the oligos randomly at our facility.




We are offering:


Standard Oligos

Long Oligos

Phosphorothioate Oligos (S-Oligo)

Modified Oligos

Fluorescent Oligos


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Jack Lan

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