BATJ Inc.

• Cloning into Expression pET Vector:
  • Clone (subclone) interest gene into a pET expression vector. The cloned gene will be verified by sequencing. The final editing sequence data and mini-prep DNA will be sent back to the customer.
    Please provide 1 ug of containing the gene of interest plasmid DNA and complete vector sequence in electronic format.
• Identification of Expression Positive Clones:
  • Transfer pET plasmid containing the gene into a BL21 (DE3).Over-express target small scale protein. Test recombinant protein by SDS-PAGE and western Blot (customer must provide appropriate antibodies).
    Please provide 1 ug of pET plasmid containing interest gene and complete vector sequence in electronic format.
• Large-scale Culture:
  • Make one liter or more of bacterial culture induced with an IPTG inducer and harvest cell pellet for protein purification.
    Please provide expression positive clone(E.coli glycerol stock or plate)

• Clone an insert of interest into a vector provided by the customer. The cloned gene will be sequenced upon completion for verification. Sequencing data and mini-prep will be sent back to the customer. Other prep services are available at additional cost.

BATJ offers high quality longer oligos (up to 120 bases) for gene synthesis, cloning, mutagenesis quick change, primer walking sequencing, and PCR applications. Our synthesis machine has a real time trityl monitor which guarantees product quality and no lost bases during synthesis. Primer prices depend on the scale, number of bases, and purification level. Turnaround time is normally 24 hours, two additional days for PAGE purification and HPLC purification.

• Site-directed mutagenesis for any combination of mutation within a 4 base-frame. The price includes quick change reaction, transformation, mini-prep and sequencing verification.
• Turnaround time is 3 to 5 workdays in most cases.
• DNA template and identified sequencing data will be sent back to customer.

• BATJ Inc.'s excellent protocol for fragment analysis is performed with 3730xl DNA Analyzer.

• Fragment Analysis Applications include:

  • Microsatelite Analysis
  • SNP Genotyping
  • Amplified Fragment Length Polymorphism (AFLP)

• Currently, we support the following Dye Matrices and Size Standards:

  • DS-33: 6-FAM (Blue), VIC (Green), NED (Yellow), PET (Red), and LIZ-500 (Orange) size standard.
  • DS-30: 6-FAM (Blue), HEX (Green), NED (Yellow), and ROX-500 (Red) for size standard.

• Turnaround time is 24 hours for fragment analysis. Free pick up is offered for local companies.

• Analysis ".fsa" data and/or text files will be sent via email.

BATJ offers a comprehensive range of next generation sequencing services utilizing Thermo Fisher's Ion PGM and Illumina's HiSeq 2500 platforms to deliver fast, reliable and high quality results. HiSeq 2500 can produce over 300 million reads with "Rapid Run" mode, and 2 billion reads with "High-Output" mode. It is much better for RNA-SEQ, CHIP-Seq, Exome-Seq and many others. Detail of NGS service is on Flyer of NGS Service.

NGS applications of Ion PGM : Ion Ampliseq (50-gene Cancer panel etc.); Metagenomic Sequencing (16s rRNA); Targeted Resequencing; SNP Discovery and Verification; Genotyping By Sequencing; Antibody Repertoire Sequencing (Ig-seq) and so on.

***BATJ also developed a special low-cost and high-throughput sequencing service for whole plasmids or PCR fragments (PPS service). The advantages of PPS service over conventional ones are:

1. Determine the complete sequence of circular or linear DNA molecules in a single run, no more walking primers to design and synthesis

• Ideal for unknown plasmid structure or whole plasmid identification after modification
• Suitable for a wide range of difficult templates (repetitive regions, strong secondary structures, GC rich )
• Reference sequence preferred, but not required
• Deep coverage of the whole DNA sequence (>100x)
• Up to 96 barcoded samples per chip run
• Fast turnaround time (usually 3-5 business days)

NGS applications of HiSeq-2500 :Whole-Transcriptome Sequencing (RNA-Seq), mRNA-Seq, de novo Genome Sequencing, Metagenomic Sequencing, Copy Number Analysis, DNA-Protein Interaction Analysis (ChIP-Seq), DNA Methylation analysis.

• Long range (LR) DNA sequencing done with the newest version of the ABI 3730 capillary machine, readable bases up to 1,000 and longer. DNA sequencing price depends on the service.
• Turnaround time is 24 hours for most sequencing. Free pick up is offered for local companies, if the order is over five reactions.
• Quality Guarantee: If desired, one free sequencing repeat is offered per reaction.
• Sequencing trace data and/or text files will be sent via email.