Axol Bioscience specialize in the supply of human cell culture systems and custom services for disease modeling and drug discovery. Our expertise includes induced pluripotent stem cell (iPSC) generation, CRISPR-Cas9 gene editing, iPSC differentiation and custom cell sourcing.
We offer a variety of functionally validated human primary cells and human iPSC-derived cells from both healthy and patient donors alongside culture media and reagents that are tailored to support the optimal growth and maturation of our cells. This enables you to carry out your project in a physiologically relevant system that is not only reliable and easy to use but produces consistent results for robust replication studies. Our team is passionate about great science, delivering superb customer service and support and innovating future products to help customers advance their research faster. At Axol, innovation, quality and customer service are key.
We specialise in the supply of human cell culture systems and custome services for disease modeling and drug discovery.
Our expertise includes human induced pluripotent stem cell (hiPSC) generation, as well as providing a variety of custom services to support your individual research needs.
Our aim is to provide you with cutting-edge, physiologically relevant cellular models and systems that are reliable and easy to use, helping you to generate high quality, consistent results with your in vitro studies
Finding the right cells for your experiments can be difficult, especially when studying rare diseases or when specific donor inclusion/exclusion criteria are required.
On top of this, it can be particularly tough to source primary human cells and tissues, but these physiologically relevant cellular models can better reflect the biology of the tissue or disease you are studying.
So, let us find the cells and tissues for you.
By listening carefully to your needs, we can source biological specimens, such as blood and tissues, for a wide range of clinical indications, including autoimmune, neurological, pulmonary, cardiovascular, skin, kidney and bladder disorders as well as liver cirrhosis.
We also specialize in sourcing primary cancer tissue for a broad range of cancers. Many of the primary cancer tissue samples are collected prior to treatment, so the tissue is treatment-naïve. This reduces potential unexpected variables when studying the effects of anti-cancer compounds on primary cancer cells.
All our biospecimens are:
✓ Obtained with fully informed consent from the donor or the donor‘s next-of-kin, with ethics committee or IRB approval
✓ Screened for human pathogens such as HIV and hepatitis viruses
✓ Provided as tissue samples either freshly frozen or formalin-fixed and paraffin-embedded
What we can do for you
Our custom cell and tissue sourcing services cover all the potential needs:
✓ Provide physiologically relevant cellular models that better reflect the biology of the tissue or disease you are studying
✓ Source specific donor cells and tissues (from more than 40 tissue types) from healthy donors and patients across a wide variety of disease indications
✓ Source specific donor cells and tissues from patients across more than 100 disease types
✓ Provide cells and tissues that meet all the data protection and ethical standards required in the UK
What we can do for you
Our reprogramming service includes:
✓ Recovery, passaging, initial testing and banking of donor fibroblasts or PBMCs
✓ Cell reprogramming with non-integrating, zero-footprint systems:
i. Episomal vectors or
ii. CytoTune™-iPS 2.0 Sendai virus
✓ Analysis of pluripotency using immunocytochemistry and TaqMan hiPSC Scorecard Assay
Additional 'add-on' services include:
✓ Human pathogen testing
✓ Short-Tandem Repeat (STR) profiling
✓ qPCR analysis for pluripotency markers
✓ Karyotyping (G-Banding)
✓ Provision of more than one iPSC colony from a single donor
✓ Assesment of reprogramming vector clearance
✓ Checking expression of additional marker genes by immunocytochemistry
✓ Sequencing genomic regions of interest
✓ Sourcing donor fibroblasts and PBMCs
✓ Adapting your iPSCs from a feeder layer to a feeder-free system
✓ iPS cell diffferentiation to multipe important cell lineages and supply of ready-to-use/cryopreserved cells
✓ Genome editing and creation of isogenic cell lines with CRISPR-Cas9
Genetically edited iPSC-derived models can help researchers better understand how specific genes contribute to disease pathogenesis at the molecular and cellular level in a tissue-specific nature.
Furthermore, they represent valuable platforms for identifying drug targets and studying drug mechanisms of action, paving the way for developing new therapeutic interventions for many genetic diseases that are currently untreatable. Conducted in partnership with Horizon Discovery, our custom cellular reprograming service and directed differentiation of iPSCs offers the possibility of generating many disease models from any genetic background.
By combining the power of CRISPR-Cas9 genome editing from Horizon Discovery with our iPSC technology, we can create accurate cellular models of genetic conditions by introducing disease-causing mutations into healthy control cell lines.
We can also correct those mutations in genomes of patient-derived cells to provide the matched control cell line.
Our custom iPSC genome editing service includes:
✓ Optimized and highly efficient gene editing workflow for iPSCs
✓ Screening a selection of gRNAs and resulting cell clones
✓ Sequence verification of genotypes engineered:
✓ Homozygous and heterozygous knockouts
✓ Homozygous and heterozygous point mutations
✓ Tagged reporter cell lines
✓ Pluripotency assessment of gene-edited cell lines
We provide cost-effective services to generate multiple cell types from your iPSCs. Our protocols are tailored for large scale production.
The majority of our culture conditions are fully defined in order to achieve both a high differentiation efficiency, and level of purity whilst retaining minimal lot-to-lot variation.
The turnaround time for directed differentiation is approximately 2 months. at least three frozen vials of iPS cells per sample will be needed. One vial will be thawed and tested for mycoplasma. We will start the differentiation process once the quarantine is completed.
Once you receive the differentiated cells from us, our dedicated specialists will provide ongoing support to help ease the adaptation of the cells into your experimental systems.
Our iPSC differentiation capabilities include:
✓ Directed differentiation of iPSCs to many cell types:
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