1335914993000

Australian Proteome Analysis Facility

Macquarie Park, New South Wales, AU

APAF Ltd - Australian Proteome Analysis Facility, was the birthplace of the term proteomics in 1995 and was the world's first dedicated high throughput proteomics laboratory. APAF has over 15 years of experience in providing proteomic services, and combined with our leading edge infrastructure and expertise we provide total solutions for all your proteomic research needs.

APAF's mission is to assist the scientific community address their protein analysis needs.

Australian Proteome Analysis Facility has not listed any services.

PAGE
Polyacrylamide Gel Electrophoresis
Starting at $120.00 per gel

11cm mini 2-D gel - Fluorescent stained - Run gel, any pH range, stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner $180 per gel

11cm mini 2-D gel - Coomassie stained - Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner $120 per gel

17 or 18... Show more »

11cm mini 2-D gel - Fluorescent stained - Run gel, any pH range, stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner $180 per gel

11cm mini 2-D gel - Coomassie stained - Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner $120 per gel

17 or 18 cm format 2-D gel - Fluorescent stained - Run gel, any pH range, gel stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner $350 per gel

17 or 18 cm format 2-D gel - Coomassie stained - Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner $200 per gel

24 cm format 2-D gel - Fluorescent stained - Run gel, any pH range, gel stain with SYPRO Ruby or Flamingo Pink, or Lava Purple, etc., image capture with a Typhoon scanner $400 per gel

24 cm format 2-D gel - Coomassie stained - Run gel, any pH range, gel stain with Coomassie, image capture with a flatbed scanner $250 per gel

« Show less
2D gel image analysis
Starting at $100.00 per hour

Image analysis is extracting tangible data out of the 2DE image with specially designed software.

Typically it involves detecting spots and warping separate images to align like-spots of the same protein. Spot data comes from the level of spot darkness which is proportional to the level of protein staining or dye labeling of... Show more »

Image analysis is extracting tangible data out of the 2DE image with specially designed software.

Typically it involves detecting spots and warping separate images to align like-spots of the same protein. Spot data comes from the level of spot darkness which is proportional to the level of protein staining or dye labeling of particular amino acids.

Gel Image Analysis/Differential display analysis - Image analysis using Progenesis software, interpretation of results and report writing - $100 per hour or ask for a quote for bulk

« Show less
Amino acid analysis
Starting at $135.00 per sample

APAF has refined Amino Acid Analysis methodologies and Free Amino Acids ensuring accurate and reliable results from a broad range of sample types including:

food stuffs
traditional medicines
recombinant protein sources
plant materials

The pricing ranges based on what you require but for standard amino acid analysis of the... Show more »

APAF has refined Amino Acid Analysis methodologies and Free Amino Acids ensuring accurate and reliable results from a broad range of sample types including:

food stuffs
traditional medicines
recombinant protein sources
plant materials

The pricing ranges based on what you require but for standard amino acid analysis of the 16 Amino Acids (does not include Cysteine or Tryptophan and Asparagine and Glutamine convert to their acid forms) the price is AU$135

« Show less
DNA/RNA Analysis and Quality Control
Price on request
Request a quote for more information about this service.
Agilent 2100 Bioanalyzer
Bioinformatics
Starting at $150.00 per hour

APAF employs commercially available and in-house built state-of-the-art bioinformatics tools for analysing proteomics data. We have terabytes of storage space for the vast amount of data that proteomics experiments generate.

For analysing iTRAQ/SILAC data, we use:

AB Sciex ProteinPilot
For gel image analysis, we... Show more »

APAF employs commercially available and in-house built state-of-the-art bioinformatics tools for analysing proteomics data. We have terabytes of storage space for the vast amount of data that proteomics experiments generate.

For analysing iTRAQ/SILAC data, we use:

AB Sciex ProteinPilot
For gel image analysis, we use:

Nonlinear Dynamics Progenesis
Bio-Rad PDQuest
For protein identification we have the following in-house search engines:

Matrix Science Mascot Search Engine
Thermo Scientific Sequest Search Engine and BioWorks Browser
The GPM Organisation X! Tandem Search Engine
NCBI OMSSA Search Engine

« Show less
Bradford assay
Starting at $150.00 per sample

$150 per batch (1 to 72 wells per batch)

$150 per batch (1 to 72 wells per batch)

« Show less
DIGE
Differential gel electrophoresis
Starting at $600.00 per gel

Difference Gel Electrophoresis (DIGE) saturation labeling - Sample labelling with Cy3 and Cy5, run gel, image capture with a Typhoon scanner - $600 per gel (up to 4 gels)

Difference Gel Electrophoresis (DIGE) minimal labeling - Sample labelling with Cy2, Cy3 and Cy5, run gel, image capture with a Typhoon scanner $1,200 per gel (up to 4 gels)

Difference Gel Electrophoresis (DIGE) saturation labeling - Sample labelling with Cy3 and Cy5, run gel, image capture with a Typhoon scanner - $600 per gel (up to 4 gels)

Difference Gel Electrophoresis (DIGE) minimal labeling - Sample labelling with Cy2, Cy3 and Cy5, run gel, image capture with a Typhoon scanner $1,200 per gel (up to 4 gels)

« Show less
ESI-MS
Electrospray Ionization Mass Spectrometry
Starting at $220.00 per sample

Mass Analysis of Intact Protein by MALDI or direct infusion ESI MS - includes MS data acqusition and report

$220 for the first sample, $125/sample for second or more samples

Mass Analysis of Intact Protein by MALDI or direct infusion ESI MS - includes MS data acqusition and report

$220 for the first sample, $125/sample for second or more samples

« Show less
LC-ESI-MS/MS
Liquid Chromatography Coupled Electrospray Ionization Tandem Mass Spectrometry
Price on request

1D NanoLC ESI MS/MS analysis for Protein Identification:

The sample is digested with trypsin and loaded onto a Capillary LC system coupled to an MS/MS instrument. The peptides are separated using a reversed phase C18 column and directly eluted into the MS. The ions entering the MS are continuously sampled and when a peptide is... Show more »

1D NanoLC ESI MS/MS analysis for Protein Identification:

The sample is digested with trypsin and loaded onto a Capillary LC system coupled to an MS/MS instrument. The peptides are separated using a reversed phase C18 column and directly eluted into the MS. The ions entering the MS are continuously sampled and when a peptide is detected, the MS switches to MS/MS mode and automatically fragments the peptide. When the run is completed, software (e.g Mascot) is used to interrogate protein, DNA or EST databases and identify the protein(s). Where the protein of interest is not in the database, de-novo sequencing and Blast searching can be used to identify peptides similar to those in known proteins.

2D LC ESI MS/MS Analysis for Protein Identification:

The sample is digested with trypsin. The digest is then loaded onto a strong cation ion exchange (SCX) column. The fractions from the SCX chromatograph are collected and further separated using a reversed phase C18 column which is coupled with an ESI mass spectrometer. The ions entering the MS are continuously sampled and when a peptide is detected, the MS switches to MS/MS mode and automatically fragments the peptide. When the run is completed, software (e.g Mascot) is used to interrogate protein, DNA or EST databases and identify the protein(s).

« Show less
Edman degradation
Starting at $223.00 per sample

Edman sequencing set-up per sample $190
Edman sequencing of each amino acid residue cycle $33

Edman sequencing set-up per sample $190
Edman sequencing of each amino acid residue cycle $33

« Show less
ESI-TOF-MS
Electrospray ionization time of flight mass spectrometry
Price on request

The intact protein is analysed by ESI-TOF MS.This is recommended as it is more accurate and requires less sample. Proteins are treated in a variety of ways depending on the sample and the MS method used. Once analysed the result is reported as a mass, plus or minus the experimental error.

The intact protein is analysed by ESI-TOF MS.This is recommended as it is more accurate and requires less sample. Proteins are treated in a variety of ways depending on the sample and the MS method used. Once analysed the result is reported as a mass, plus or minus the experimental error.

« Show less
Gel Staining
Price on request
Request a quote for more information about this service.
Glycan analysis
Price on request

Glycosylation is one of the most universal and structurally diverse post-translational modification (PTM) with more than half of all mammalian proteins known to be glycosylated. The significance of correct glycosylation in various biological events such as protein secretion, stability and immunogenicity and the direct correlation... Show more »

Glycosylation is one of the most universal and structurally diverse post-translational modification (PTM) with more than half of all mammalian proteins known to be glycosylated. The significance of correct glycosylation in various biological events such as protein secretion, stability and immunogenicity and the direct correlation of aberrant glycosylation with diseases like cancer and congenital disorders of glycosylation are well documented. Identification and characterisation of glycans together with their particular protein carriers is crucial for deciphering glycan functionality in normal and diseased conditions. APAF has a documented record of glycan analysis with leading biotechnology firms.

« Show less
HPLC
High Performance Liquid Chromatography
Price on request
Request a quote for more information about this service.
High abundant protein removal
Price on request

Removal of high abundance protein using Agilent MARS and Genway columns.

Removal of high abundance protein using Agilent MARS and Genway columns.

« Show less
Agilent MARS
Genway
Ion Exchange Chromatography
Starting at $200.00 per sample

Price on a per sample basis and there may be some development cost.

Price on a per sample basis and there may be some development cost.

« Show less
LC-MS
Liquid Chromatography-Coupled Mass Spectrometry
Starting at $150.00 per hour

instrument run time

instrument run time

« Show less
LC-ESI-MS/MS
Liquid Chromatography Coupled Electrospray Ionization Tandem Mass Spectrometry
Starting at $350.00 per sample

ID nanoLC ESI MS/MS for Protein Identification; - Trypsin digestion, reverse phase nanoLC ESI MS data acqusition, database search and report

1 - 5 samples $350/sample
6+ samples $330/sample

ID nanoLC ESI MS/MS for Protein Identification; - Trypsin digestion, reverse phase nanoLC ESI MS data acqusition, database search and report

1 - 5 samples $350/sample
6+ samples $330/sample

« Show less
Luminex Multiplex Assays
Price on request

APAF can offer analysis using commercially available kits on a BioRad Bioplex and robotic system.

Multiplex assays of biological fluids using Luminex 100 system
Luminex 100 IS system is developed based on xMAP® technology and it is a flexible analyzer based on the principles of flow cytometry. The system enables to multiplex... Show more »

APAF can offer analysis using commercially available kits on a BioRad Bioplex and robotic system.

Multiplex assays of biological fluids using Luminex 100 system
Luminex 100 IS system is developed based on xMAP® technology and it is a flexible analyzer based on the principles of flow cytometry. The system enables to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. This gives opportunities for performing quick and cost-effective bioassays, while ensuring optimum accuracy.

« Show less
MALDI TOF MS
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Starting at $170.00 per sample

4800 MALDI-TOF/TOF (PMF+MS/MS) for Protein Identification; 1 - 96 samples 1

Include trypsin digestion, zip tipping clean up and concentrating samples on AnchorChip plate, MS data acqusition, database search and report

$170/sample, maximum $6300

4800 MALDI-TOF/TOF (PMF+MS/MS) for Protein Identification; 1 - 96 samples 1

Include trypsin digestion, zip tipping clean up and concentrating samples on AnchorChip plate, MS data acqusition, database search and report

$170/sample, maximum $6300

« Show less
Mass spectrometry data analysis
Starting at $150.00 per hour
Request a quote for more information about this service.
MudPIT Analysis
Multidimensional Protein Identification Technology
Price on request
Request a quote for more information about this service.
MRM/SRM
Multiple Reaction Monitoring/Selected Reaction Monitoring Mass Spectrometry
Price on request

MRM/SRM Assay Development
Method development for targeted proteins or peptides

MRM/SRM Sample Analysis
Using the developed method to acquire data for the targeted proteins or peptides, data analysis, report

MRM/SRM Assay Development
Method development for targeted proteins or peptides

MRM/SRM Sample Analysis
Using the developed method to acquire data for the targeted proteins or peptides, data analysis, report

« Show less
Nano-LC-MS - Short Gradient
Nano Liquid Chromatography Coupled Mass Spectrometry - Short Gradient
Price on request

Phosphopeptide analysis by nano-LC/MS/MS:
The analysis of phosphopeptides from complex protein mixtures remains a challenging procedure. APAF uses an Applied Biosystems 4000 Q-Trap hybrid quadrupole linear ion-trap instrument. This instrument is well suited to the analysis of post-translational modifications as diagnostic... Show more »

Phosphopeptide analysis by nano-LC/MS/MS:
The analysis of phosphopeptides from complex protein mixtures remains a challenging procedure. APAF uses an Applied Biosystems 4000 Q-Trap hybrid quadrupole linear ion-trap instrument. This instrument is well suited to the analysis of post-translational modifications as diagnostic fragment ions can be detected by precursor ion scanning. In this scan type, Q1 is scanned across a mass range and ions are fragmented in Q2. Q3 is set to transmit only the mass of the diagnostic fragment, (-79 m/z in the case of a phosphopeptide). In a follow-up experiment, precursor ions are selected from fragmentation and product ions scanned out of the ion-trap to provide MS/MS sequence information. Often the site of phosphorylation can be determined from this spectra.

« Show less
Peptide mapping
Price on request

De Novo Peptide Sequencing - Data analysis only. Sample prep and MS data acqusition extra.
$75/peptide

De Novo Peptide Sequencing - Data analysis only. Sample prep and MS data acqusition extra.
$75/peptide

« Show less
Phosphoproteomics
Price on request

Phosphoproteomics refers to the global analysis of the phosphate modified proteome. Typically phosphorylated amino acids include serine, threonine, and tyrosine and at APAF we have established methods to enrich and analyse peptides containing these modified residues. For phospho-serine/threonine peptides we use a combination of... Show more »

Phosphoproteomics refers to the global analysis of the phosphate modified proteome. Typically phosphorylated amino acids include serine, threonine, and tyrosine and at APAF we have established methods to enrich and analyse peptides containing these modified residues. For phospho-serine/threonine peptides we use a combination of ion exchange chromatography and titanium dioxide enrichment prior to LC/MS. For phosphotyrosine containing peptides immunoaffinity capture is used.

« Show less
Post-Translational Modification Analysis
Price on request
Request a quote for more information about this service.
Protein Purification
Starting at $100.00 per sample

Sample preparation from tissue for 2-DE:
Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel $150 for the first sample; $50/sample for second or more samples

Sample preparation from microorganisms for 2-DE:
Extraction of proteins, desalting, quantitation by Bradford assay and validation... Show more »

Sample preparation from tissue for 2-DE:
Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel $150 for the first sample; $50/sample for second or more samples

Sample preparation from microorganisms for 2-DE:
Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel $150 for the first sample; $50/sample for second or more samples

Sample preparation from plants for 2-DE: Extraction of proteins, desalting, quantitation by Bradford assay and validation on 1-D gel $200 for the first sample; $75/sample for second or more samples

Sample preparation from human body fluids (+/- depletion): Concentration, desalting, quantitation by Bradford assay and validation on 1-D gel $100 for the first sample; $50/sample for second or more samples

« Show less
Receptor Binding Assays
Price on request

APAF provides services for ‘Functional Proteomics’, the purification of protein bioactives and their assessment for biological activity as indicated by cellular activation or modulation. These capabilities will be underpinned by the purchase and implementation of state-of-the-art protein purification equipment and methodologies,... Show more »

APAF provides services for ‘Functional Proteomics’, the purification of protein bioactives and their assessment for biological activity as indicated by cellular activation or modulation. These capabilities will be underpinned by the purchase and implementation of state-of-the-art protein purification equipment and methodologies, and the development of cell-based high throughput screening assays of biological activity. The cell-based assays will utilize measurement of cellular signal transduction pathways as the index of receptor activation, inhibition or modulation. Development of these assays is at an advanced stage at TGR BioSciences, and it is anticipated that a limited service utilizing these technologies will be available by the end of the calendar year or early 2003. The assays utilize changes in protein states (such as enzymes) as the readout of cellular activation status. The number and further implementation of these assays will be expanded in the coming calendar year.

« Show less
Western Blot
Starting at $300.00 per gel

Western analysis (1-D gel, up to 10 wells) - using client supplied primary antibody:
Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera - $300 per Western blot

Western analysis (1-D gel, up to 18 wells) - using client supplied primary antibody: Run... Show more »

Western analysis (1-D gel, up to 10 wells) - using client supplied primary antibody:
Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera - $300 per Western blot

Western analysis (1-D gel, up to 18 wells) - using client supplied primary antibody: Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera - $325 per Western blot

Western analysis (11 cm 2-D gel) - using client supplied primary antibody:
Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera - $360 per Western blot

Western analysis (17 cm 2-D gel) - using client supplied primary antibody: Run gel, transfer proteins to membrane, probe with antibodies, ECL detection and image capture with a Fuji LAS 3000 CCD camera - $450 per Western blot

« Show less
iTRAQ Protein Labeling
Isobaric Tags for Relative and Absolute Quantitation
Price on request

Quantitation usng iTRAQ™ reagents and nano-LC/MS/MS

iTRAQ™ reagents are isobaric amine-specific reagents that allow relative quantitation of up to four different samples in a single MS/MS experiment. iTRAQ™ reagents eliminate the need of conducting separate MS experiments to compare expression changes. A further advantage is... Show more »

Quantitation usng iTRAQ™ reagents and nano-LC/MS/MS

iTRAQ™ reagents are isobaric amine-specific reagents that allow relative quantitation of up to four different samples in a single MS/MS experiment. iTRAQ™ reagents eliminate the need of conducting separate MS experiments to compare expression changes. A further advantage is that quantitative values are obtained for all identified proteins from each of the four mixed analytes - meaning there are no "gaps" in data tables. iTRAQ™ experiments can usually conducted via LC/MS/MS, with the preferred approach utilizing a 2D-nanoLC/MS/MS system and Q-STAR mass spectrometer. For more technical information regarding iTRAQ™ visit (www.appliedbiosystems.com).

« Show less
Mass Spectrometry
Price on request
Request a quote for more information about this service.
Spectroscopy
Price on request
Request a quote for more information about this service.
Omics
Price on request
Request a quote for more information about this service.
Chromatography
Price on request
Request a quote for more information about this service.
Data Analysis and Management
Price on request
Request a quote for more information about this service.
Protein Expression Visualization
Price on request
Request a quote for more information about this service.
Electrophoresis
Price on request
Request a quote for more information about this service.
Protein Sequencing
Price on request
Request a quote for more information about this service.
Biochemical Assays
Price on request
Request a quote for more information about this service.
Gel Electrophoresis
Price on request
Request a quote for more information about this service.
Protein Services
Price on request
Request a quote for more information about this service.
Biology
Price on request
Request a quote for more information about this service.
Protein Characterization Services
Price on request
Request a quote for more information about this service.
Biostatistics & Bioinformatics
Price on request
Request a quote for more information about this service.
Protein Quantification
Price on request
Request a quote for more information about this service.
Purification Services
Price on request
Request a quote for more information about this service.
Proteomics
Price on request
Request a quote for more information about this service.
Imaging & Spectroscopy
Price on request
Request a quote for more information about this service.
Liquid Chromatography
Price on request
Request a quote for more information about this service.
Protein Data Analysis
Price on request
Request a quote for more information about this service.
Protein Purification and Quantification
Price on request
Request a quote for more information about this service.
Image Analysis
Price on request
Request a quote for more information about this service.
Size Exclusion Chromatography
Price on request
Request a quote for more information about this service.
Protein Depletion
Price on request
Request a quote for more information about this service.
Ionization/Fragmentation Methods
Price on request
Request a quote for more information about this service.
MALDI Mass Spectrometry Methods
Price on request
Request a quote for more information about this service.
Tandem Mass Spectrometry Methods
Price on request
Request a quote for more information about this service.
Liquid Chromatography Coupled Mass Spectrometry Methods
Price on request
Request a quote for more information about this service.
Chemistry and Materials
Price on request
Request a quote for more information about this service.
DNA
Price on request
Request a quote for more information about this service.
Pharmacology & Toxicology
Price on request
Request a quote for more information about this service.
Analytical Chemistry Services
Price on request
Request a quote for more information about this service.
Bioanalysis
Price on request
Request a quote for more information about this service.
Receptor Assays
Price on request
Request a quote for more information about this service.
Computational & Statistical Analysis
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Nano Mass Spectrometry Methods
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
Ask A Question Find what you're looking for? If not, you can ask this lab a question:

Australian Proteome Analysis Facility has not received any ratings.

Be the first to endorse Australian Proteome Analysis Facility

Endorse this lab