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ATCC

1 Order Completed
Manassas, Virginia, US

About ATCC

ATCC is the premier global biological materials resource and standards organization offering an extensive collection of products and services manufactured under ISO certification and accreditation.

ATCC offers services and custom solutions which include cell and microbial culturing and authentication,... Show more »

ATCC is the premier global biological materials resource and standards organization offering an extensive collection of products and services manufactured under ISO certification and accreditation.

ATCC offers services and custom solutions which include cell and microbial culturing and authentication, development and production of controls and derivatives, derivation and expansion of Primary Cells, Cell Immortalization and CRISPR gene editing services.

New Cell Authentication Testing Services include Mycoplasma Testing using PCR as well as direct and indirect methods. STR profiling is currently available for human cell authentication.

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Certifications & Qualifications

ISO 13485 ISO 17025 ISO 9001

Our Services (13)


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Assay Ready Cells

Price on request

Assay-Ready cells are frozen mammalian cells that are provided at the concentration needed and are ready to use without having to culture, passage, or maintain. Just thaw and go!

With more than 50 years experience in the production, authentication, storage, and distribution of mammalian cell lines ATCC is the solution for... Show more »

Assay-Ready cells are frozen mammalian cells that are provided at the concentration needed and are ready to use without having to culture, passage, or maintain. Just thaw and go!

With more than 50 years experience in the production, authentication, storage, and distribution of mammalian cell lines ATCC is the solution for Assay-ready cells that can be used in applications such as cell based assays, screening, or toxicology studies.

We can produce continuous or primary cells at the concentration and quantity you need!

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Knock-In Cell Lines

Price on request

CRISPR/Cas9 genome editing technology is one of the most outstanding scientific breakthroughs in recent years, revolutionizing basic and medical research by enabling site-specific genome engineering of cell lines. ATCC has previously employed CRISPR/Cas9 technology to generate isogenic cell lines with disease-relevant mutations... Show more »

CRISPR/Cas9 genome editing technology is one of the most outstanding scientific breakthroughs in recent years, revolutionizing basic and medical research by enabling site-specific genome engineering of cell lines. ATCC has previously employed CRISPR/Cas9 technology to generate isogenic cell lines with disease-relevant mutations (e.g. ATCC® CRL-1619IG-2™ and ATCC® CCL-185IG™). We now offer custom genome editing services that span from assay design to cell line development, and we will work with you to develop a cell line that best meets your research needs.

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CRISPR/Cas9 Knockout Cell Lines

Price on request

CRISPR/Cas9 genome editing technology is one of the most outstanding scientific breakthroughs in recent years, revolutionizing basic and medical research by enabling site-specific genome engineering of cell lines. ATCC has previously employed CRISPR/Cas9 technology to generate isogenic cell lines with disease-relevant mutations... Show more »

CRISPR/Cas9 genome editing technology is one of the most outstanding scientific breakthroughs in recent years, revolutionizing basic and medical research by enabling site-specific genome engineering of cell lines. ATCC has previously employed CRISPR/Cas9 technology to generate isogenic cell lines with disease-relevant mutations (e.g. ATCC® CRL-1619IG-2™ and ATCC® CCL-185IG™). We now offer custom genome editing services that span from assay design to cell line development, and we will work with you to develop a cell line that best meets your research needs.

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Primary Cell Line Generation

Price on request

Custom Primary Cell Derivation

ATCC’s Cell Derivation Unit produces custom primary cells allowing the capability to study tissue specific characteristics on a cellular level in vitro and to develop more biologically relevant models. Healthy
cells, as well as cells derived from diseased tissues such as asthma, COPD,... Show more »

Custom Primary Cell Derivation

ATCC’s Cell Derivation Unit produces custom primary cells allowing the capability to study tissue specific characteristics on a cellular level in vitro and to develop more biologically relevant models. Healthy
cells, as well as cells derived from diseased tissues such as asthma, COPD, pulmonary fibrosis, diabetes and many other pathologies can be produced. For each cell type, careful consideration is given
to maintain broad donor diversity, robust quality control, lot-to lot consistency and large scale production capacity. Laboratories that may have started to isolate primary cell culture in-house face challenges with lots not being standardized therefore, may be unable to perform inter-laboratory studies and comparisons due to variability among the reagents and procedures used to derive primary cells.

ATCC’s project management team can partner with you to develop custom protocols by working to understand your needs, from starting tissue requirements, production, scalability, automated vialing, to storage and distribution.

WHAT WE OFFER
▪ Custom primary cells can be produced in large lots, making them ideal for scale up as ready-to-use cells or ready-to-store for longer term studies
▪ Flexibility, by removing time dependencies with tissue procurement and isolations. All tissues utilized for human primary cell derivation are ethically obtained with documented, legal permission for research use. Upon request, detailed donor information can be provided both for normal and diseased cells
▪ Characterization with full quality control testing that can include phenotyping by immunocytochemistry (ICC) or flow cytometry
▪ Offering reagents matched and optimized to work with each primary cell type

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Cell Immortalization

Price on request

Immortalized cell lines offer extended proliferative capacity in vitro, while closely mimicking the physiology of cells in vivo. Unlike primary cells, immortalized cells are not susceptible to replicative senescence, reducing the need to repurchase and re-initiate growth, saving time and expense.
ATCC offers cell immortalization... Show more »

Immortalized cell lines offer extended proliferative capacity in vitro, while closely mimicking the physiology of cells in vivo. Unlike primary cells, immortalized cells are not susceptible to replicative senescence, reducing the need to repurchase and re-initiate growth, saving time and expense.
ATCC offers cell immortalization services using various technologies such as human telomerase reverse transcriptase ( hTERT), which provides stable genotypes of diverse cell types and tissue sources. Immortalized cell lines have an extended lifespan and typically are karyotypically, morphologically and phenotypically similar to the primary parent cells.

WHAT WE OFFER
▪ Custom immortalization services produce cell lines, which have an extended lifespan and typically are similar to the primary parent cells
▪ The ability to overcome the limited lifespan of primary cells by combining our custom cell derivation service with our custom cell immortalization service
▪ In addition to standard ATCC cell authentication testing, immortalized cell lines can be tested for extended proliferative capacity and selected phenotypic markers from the tissue of interest (e.g., the continuous expression of hTERT)
▪ Experienced project managers and subject matter experts will work with you to produce an immortalized cell line tailored to your requirements

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Cell Expansion

Price on request

Working cell banks (WCBs) are prepared from the MCB under defined cell culture conditions. Quality control tests, via DNA profiling techniques, are performed to confirm that the MCB and the WCB are genetically identical, as well as to ensure the WCB is free of contaminants.

Custom Working Cell Bank Development Service... Show more »

Working cell banks (WCBs) are prepared from the MCB under defined cell culture conditions. Quality control tests, via DNA profiling techniques, are performed to confirm that the MCB and the WCB are genetically identical, as well as to ensure the WCB is free of contaminants.

Custom Working Cell Bank Development Service Description:

Procurement of starting material/cells (source can be ATCC, commercial vendors, or customer)
Development of product specifications (e.g. product description, total cell count concentration – total cell/mL, minimum volume/vial, estimated passage at completion)
Working cell bank creation (expansion)
QC testing (e.g. viability, mycoplasma, sterility, etc.)
Storage and shipment (ongoing supply)

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Cell Banking

Price on request

Master Cell Bank (MCB) development is a critical process in biological materials production. Since the quality of your final product is dependent on the quality of the cells used for its production, great care should be taken while developing master cell banks.

Many things can go wrong with MCB development. The use of improper... Show more »

Master Cell Bank (MCB) development is a critical process in biological materials production. Since the quality of your final product is dependent on the quality of the cells used for its production, great care should be taken while developing master cell banks.

Many things can go wrong with MCB development. The use of improper and uncharacterized cell lines, genetic variation, and cell line contamination are just a few possible problem sources. The master cell bank development services we offer address all these issues.

Custom Master Cell Bank (MCB) Development Service Description:
Pre-development

Cell line viability testing
Contamination testing
Mycoplasma
Sterility

Cell id (authenticity) testing
STR, CO1, surface expression marker testing
MCB specification development (concentration – total cells/vial, volume per vial, growth medium, freezing medium, serum, antibiotic, QC specifications)

Development

Modifications to cell lines (if required)
Cell line expansion
Selection of ideal growth medium and components
Culturing and sub-culturing

Thawing
Assessment and validation of preservation methods
Cryoprotection toxicity tests
Temperature, concentration, duration, etc.
Cooling profile investigation

Documentation of procedures, cultures, and reagents used

Post-development

Post-development QC testing
Storage (liquid nitrogen vapor phase)
Documentation of stored materials (Accession records, cell banking records, quality control records, media batch preparation records, sterilization records).

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Cell Line Characterization

Price on request

ATCC provides a range of authentication testing services designed to ensure consistency and reliability of your research data.

Cell Bank Characterization Common Tests:
Mycoplasma (PCR and direct and indirect culture assays)
Cell ID testing (STR profiling)

ATCC provides a range of authentication testing services designed to ensure consistency and reliability of your research data.

Cell Bank Characterization Common Tests:
Mycoplasma (PCR and direct and indirect culture assays)
Cell ID testing (STR profiling)

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Nucleic Acid Services

Price on request

If you can’t find the nucleic acids that you need from ATCC catalog products currently available, ask ATCC to make it for you! Small-scale quantities of nucleic acids from ATCC Genuine Cultures® are ideal for PCR, cloning or other molecular applications. If your research requires larger quantities of nucleic acids, we can grow,... Show more »

If you can’t find the nucleic acids that you need from ATCC catalog products currently available, ask ATCC to make it for you! Small-scale quantities of nucleic acids from ATCC Genuine Cultures® are ideal for PCR, cloning or other molecular applications. If your research requires larger quantities of nucleic acids, we can grow, extract and purify nucleic acids from most ATCC cultures.

Small-scale product description:

  • Small-scale (1 µg-10 µg) of DNA or RNA isolated from Animal Cell Lines in the ATCC Cell Biology collection
  • Small-scale (50 ng-2 µg) of DNA isolated from most Bacterial strains in the ATCC Bacteriology collection

Turn-around Time: 7-10 days

Yield:

  • DNA and RNA from Animal Cell lines: ≥1 µg
  • DNA from Bacterial cells: ~ 200 ng

Release Testing:

  • DNA concentration is measured by PicoGreen®. DNA integrity is assessed by agarose gel electrophoresis
  • RNA is tested for concentration and integrity on an Agilent 2100 Bioanalyzer

Biosafety Level (BSL):

  • Custom nucleic acids ship at the BSL of the parental organism
  • Viability testing is available for an additional fee
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Microbial Strain Development

Price on request

Custom Microbial Strain Derivatives

INACTIVATED MICROBIAL CULTURES

▪ Custom inactivation of bacterial, fungal, viral and parasitic strains available
▪ Fully intact, whole genome, non-viable
▪ Inactivation is tested and verified and a Certificate of Analysis (COA) is provided
▪ Mimics a clinical specimen, can be used... Show more »

Custom Microbial Strain Derivatives

INACTIVATED MICROBIAL CULTURES

▪ Custom inactivation of bacterial, fungal, viral and parasitic strains available
▪ Fully intact, whole genome, non-viable
▪ Inactivation is tested and verified and a Certificate of Analysis (COA) is provided
▪ Mimics a clinical specimen, can be used in protocols identical to clinical samples
▪ Can be used as independent external controls to routinely monitor the performance of your workflows and assays
▪ We can provide a qualitative or quantitative format
▪ Both pre and post inactivation quantitation available by Droplet Digital™ PCR (ddPCR™)
▪ Quantitative format allows for generation of a standard curve and monitoring assay-to-assay and lot-to-lot variation

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Large Scale Microbial Culture

Price on request

Custom Microbial Manufacturing

ATCC was established in 1925 with the fi rst bacterial strain and has consistently attained
the highest standards, using the most reliable, consistent protocols to manufacture and
characterize microbial strains. We off er expert capabilities for the preparation, quality
control and... Show more »

Custom Microbial Manufacturing

ATCC was established in 1925 with the fi rst bacterial strain and has consistently attained
the highest standards, using the most reliable, consistent protocols to manufacture and
characterize microbial strains. We off er expert capabilities for the preparation, quality
control and preservation of microbial cultures, either from ATCC catalog cultures or your own
microbial cultures. Whether it is the culture expansion, quality control testing, preservation,
packaging formats or storage and distribution services, our experienced team is ready to
meet your microbial production needs.
WHAT WE OFFER
▪ Flexible manufacture of your microbial strains or ATCC strains, working with you to defi ne
and standardize culture conditions, scalability, quality control testing, preservation
methods, packaging and storage and distribution
▪ Comprehensive quality control analysis of your microbial strain by ATCC experts
▪ Scalable production from several vials up to thousands of vials
▪ Fill and fi nish of both small and large scale production with our automated vialing and labeling equipment
▪ ISO/IEC 17025:2005 accredited processes available for quality control tests, including both phenotypic and genotypic tests
▪ Storage and global distribution

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Microbial Quantification

Price on request

Custom Microbial Quantitation Solutions

ATCC offers several custom solutions to quantitate microbial strains including titering, qPCR
and Droplet Digital™ PCR (ddPCR™). These tests can be ordered separately or as part of the
quality control testing with the production of microbial banks.
Viral quantification is also... Show more »

Custom Microbial Quantitation Solutions

ATCC offers several custom solutions to quantitate microbial strains including titering, qPCR
and Droplet Digital™ PCR (ddPCR™). These tests can be ordered separately or as part of the
quality control testing with the production of microbial banks.
Viral quantification is also available to determine infectious virus particles in a sample. As
appropriate, Tissue Culture Infective Dose (TCID50), Chicken Embryo Infectious Dose (CEID50)
and the plaque assays are utilized for measuring the number of plaque forming units (PFU)
are methods used for viral titering at ATCC. Bacterial and fungal cultures are titered via
determining the number of colony forming units per milliliter (CFU/mL) and protist cell
counts are measured per volume, typically cells/mL, qPCR and Droplet Digital™ PCR (ddPCR™)
are measurements methods which measure target nucleic acid copies per input sample. If
you are developing molecular based assays and need quantification such as cfu/mL or TCID50
for analytical sensitivity, reactivity and specificity studies, please engage our experts to grow
and quantify microbial strains for you. Whether you need one or many strains grown and
quantitated, we off er the complete solution.

WHAT WE OFFER
▪ Tissue Culture Infective Dose (TCID50) quantifies the amount of virus required to produce a cytopathic effect in 50% of inoculated tissue culture cells
▪ Chicken Embryo Infectious Dose (CEID50) for viruses normally propagated in chicken eggs (such as Influenza virus) viral titer is calculated as the chicken embryo infectious dose
▪ The viral plaque assay measures the ability of a single infectious virus to form a plaque on a cell culture monolayer. The number of plaque forming units (PFU) is determined per volume of inoculum
▪ Bacterial and fungal cultures can be titered via determining the number of colony forming units per milliliter (CFU/mL)
▪ Quantitation of Chlamydia trachomatis strains at ATCC utilizes the IFU (Inclusion Forming Unit) Assay method to determine the titer. The IFU Assay determines the number of infectious units(elementary bodies) per preparation, by counting the number of inclusion bodies produced by elementary bodies in a dilution set
▪ Hemocytometers are commonly used to determine protist cell density, which is reported as the number of cell counts per volume, typically cells/mL
▪ qPCR and Droplet Digital™ PCR (ddPCR™) are quantitation methods used at ATCC for the quantitation of viral, bacterial, fungal and protist strains
▪ qPCR allows for the determination of the copy number of template DNA with accuracy and high sensitivity
▪ Quantification by genome copy number using Droplet Digital™ PCR provides an absolute count of target DNA copies per input sample without the need for running standard curves, making this technique ideal for measurements of target DNA, viral load analysis and microbial quantification
▪ Offering flexible solutions, we can produce and titer from one to several vials in ready to use frozen formats or a format of your choice.
▪ Pre and/or post freeze titers available

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Virus Production

Price on request

Virus Production Services

Virus Production Services

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