AtaGenix Laboratories specializes in researching and manufacturing customized recombinant proteins and antibodies and have extensive experience in using the five major protein expression systems to satisfy customers' various requirements.
All services are fully customised to ensure Customer's complete satisfaction
AtaGenix's customized antibody platform provides antibody engineering services such as monoclonal/polyclonal antibody customization, recombinant antibody expression, therapeutic antibody development, phage display and antibody humanization.
We also utilize our own proprietary CHO-K1 cell line in carrying out research and development of protein and antibody based drugs. AtaGenix focuses on the development of high-end protein and antibody products.
Now we have protein series such as interleukin, CD molecular, cytokines protein and industrial enzyme, and antibody series like IL molecular antibody, CD molecular antibody and zebra fish antibody
Immune or Naive Phage Display Libraries available for screening
Can develop Monoclonal Antibodies from our Phage Display Libraries
Stable transfection is the process of integrating exogenous plasmid DNAs into chromosomes of host cells, causing the host cells to express target gene and protein for long periods of time. The target cells will be screened based on transient transfection. To build stable cell lines, target cells are screened by related drugs according to the resistance marker contained in the vectors. The most commonly used eukaryotic expression vector gene markers are hygromycin, neomycin, puromycin and Hygromycin B. G418 and puromycin are commonly used for selective screening. The selected cell lines can stably express the target protein.
Provisions by AtaGenix
AtaGenix can either construct vectors or provide stable selection of the built foreign plasmid DNA. Corresponding drugs will be selected according to the resistance markers of the plasmid DNA to carry out drug test on the cells to be screened and select the lowest concentration when all the cells undergo apoptosis. Upon transient transfection, the infected cells will be screened by a suitable concentration of drugs. After 10-14 days, single clones will be picked out for amplification to detect protein expression by ELISA, SDS-PAGE and Western Blot etc. Finally, stable cell lines can be screened.
Process Flow of Stable Cell Line Service
Submission by Customers
AtaGenix provides two methods for large-scale production of monoclonal antibodies, ascites preparation and roller bottle culture.
Professional SPF animal laboratory, with sterile roller bottle culture apparatus in large scale．
Ascites can be rapidly and efficiently obtained for hybridoma cultivation．
Sophisticated technicians will handle with the purification of ascites and supernatant of hybridoma culture.
Mouse or Rabbit
Requirement of the samples provided by clients:
AtaGenix offers a one-stop service for custom antibody preparation, from antigen preparation, to antibody production, antibody labelling service as well as antibody sequencing service.
AtaGenix is able to provide clients with a tailored polyclonal antibodies service in different hosts, including rabbit, mouse, rat, guinea pig, goat and chicken.
Polyclonal Antibodies Preparation contains:
√ Antigen preparation
(1) Peptide antigen synthesis
(2) Protein antigen preparation
(3) Other antigens
√ Immunization - AtaGenix will provide an optimised and tested immunization scheme, or complete the immunisation process according to the requirements of the client
√ Antiserum production
√ Antibody purification
(1) Protein A/G purification
(2) Affinity purification
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AtaGenix Laboratories has not received any endorsements.