Asymmetrex, LLC

Asymmetrex's AlphaSTEM Test service can determine the specific count of adult tissue stem cells in complex research and clinical preparations. This capability can be used to determine the dose of stem cells in transplantation preparations, to identify drug candidates that will cause chronic organ failure because of stem cell toxicity, to identify tissue stem cell-active agents, and for general research of the cell kinetics properties of tissue stem cells, committed progenitor cells or transiently amplifying cells, and terminally differentiated cells in diverse mammalian tissues during cell culture.

The following cell kinetics parameters can be determined for the indicated specific tissue cell types in complex tissue cell cultures:

Stem Cell Kinetics Parameters
1. Number vs. time
2. Death rate vs. time
3. Cell cycle time for symmetric self-renewal
4. Cell cycle time for asymmetric self-renewal
5. Symmetric self-renewal rate
6. Asymmetric self-renewal rate
7. Stem cell unit generation number
8. Density-dependence of symmetric self-renewal rate

Non-stem Cycling Cell Kinetics Parameters
1. Number vs. time
2. Death rate vs. time
3. Cell cycle time

Terminally-Arrested Cell Kinetics Parameters
1. Number vs. time
2. Death rate vs. time

Asymmetrex's AlphaSTEM Test service can determine the specific count of adult tissue stem cells in complex research and clinical preparations. This capability can be used to determine the dose of stem cells in transplantation preparations, to identify drug candidates that will cause chronic organ failure because of stem cell toxicity, to identify tissue stem cell-active agents, and for general research of the cell kinetics properties of tissue stem cells, committed progenitor cells or transiently amplifying cells, and terminally differentiated cells in diverse mammalian tissues during cell culture.

The following cell kinetics parameters can be determined for the indicated specific tissue cell types in complex tissue cell cultures:

Stem Cell Kinetics Parameters
1. Number vs. time
2. Death rate vs. time
3. Cell cycle time for symmetric self-renewal
4. Cell cycle time for asymmetric self-renewal
5. Symmetric self-renewal rate
6. Asymmetric self-renewal rate
7. Stem cell unit generation number
8. Density-dependence of symmetric self-renewal rate

Non-stem Cycling Cell Kinetics Parameters
1. Number vs. time
2. Death rate vs. time
3. Cell cycle time

Terminally-Arrested Cell Kinetics Parameters
1. Number vs. time
2. Death rate vs. time

AlphaSTEM can design serial expansion culture parameters and schedules that increase the yield of tissue stem cells at reduced cost and time.
Requirements: total viable cell count data from serial culture expansion procedures
Approximate turnaround time: <1 week
Deliverables: 1. Serial culture expansion parameters project to increase final expanded stem cell number while reducing costs for material and labor, decreasing expansion time.
2. Estimated specific stem cell count for final product
3. Projected stem cell kinetics properties for final product
Pricing: Negotiated per Expansion Design

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