AssayGate, Inc.

We offer the most cost-effective multiplex testing services for the hundreds of protein biomarkers using the leading brand of commercial Luminex assay kits with quality assurance.

In addition to Luminex bead-based multiplex assay services which may not always be the best choice in terms of sample dilution factor, analyte-dependent matrix, antibody cross-reactivity and assay availability, etc., we also offer assay services with traditional ELISAs to detect biomarkers in samples of the following species:

• Bovine
• Chicken
• Dog
• Goat
• Guinea Pig
• Horse
• Human
• Mouse
• Porcine
• Rabbit
• Rat
• Rhesus Monkey

In addition to conventional Luminex bead-based multiplex assays and ELISAs as a broad initial analysis for biomarker profiling, our ultra-sensitive assays are established upon Simoa (Single Molecule Array) technology platform to detect low-abundance biomarkers difficult or impossible to measure with conventional assays. The digital nature of the Simoa technology allows an average of 1000 x sensitivity increase over conventional assays with CVs < 10%. Our testing lab can process barcode samples with Simoa HD-1 Analyzer which is fully automated with multiplexing and custom assay capability.

Simoa™ Ultra-Sensitive ELISAs

Simoa™ Ultra-Sensitive Multiplex Assays

Human Cytokine Panel 1

Human Cytokine Panel 2

Our endotoxin/LPS measurement is a quantitative, kinetic test based on LAL-based turbidimetric assay for the detection of Gram negative bacterial Endotoxin in a variety of sample types including serum and plasma. We offer Endotoxin/LPS testing services using the leading brand of commercial Endotoxin/LPS assay kits with quality assurance.

Our biostatistics and bioinformatics experts can guide you through this process for your biomarker data and interpret the assay results. Discriminatory biomarker analyses allow you to discover and gain critical insights into biomarkers that show statistical difference between different groups. A variety of bioinformatics analyses are also available for your protein expression data. A standard descriptive statistics (including the heatmap, unsupervised hierarchical clustering and PCA plot) can be provided as a summary of the protein expression profiling. Other analyses will be tailed on the basis of the objectives of your specific study protocol, the data availability and complexity, include but are not limited to, the identification of differential protein expression, clustering, patient stratification, pathway and function enrichment analyses.

Our scientists with decades of immunoassay development experience will work with you to understand your challenges and develop custom assay kits to accomplish your specific research goals.

Multiplex Endocrine Panels

Multiplex Human Apoptosis/Sepsis Panel

• ICAM-1
• MIF
• PAI-1 (Total)
• sFas
• sFasL
• VCAM-1

Multiplex Human Adhesion Panel

Angiogenesis Biomarker Panel