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Assay.Works

ic 9.5/10 Overall Rating 100% Positive
ic 4 Orders Completed
Regensburg, DE

About Assay.Works

Assay.Works is a private contract research organization (CRO) occupying a 750 m2 state-of-the-art laboratory located in the Regensburg BioPark, the second largest hub of the Bavarian biotech cluster. Our mission is to accelerate our partner’s research programs by developing and executing predictive and scalable... Show more »

Assay.Works is a private contract research organization (CRO) occupying a 750 m2 state-of-the-art laboratory located in the Regensburg BioPark, the second largest hub of the Bavarian biotech cluster. Our mission is to accelerate our partner’s research programs by developing and executing predictive and scalable assays to quantify the bioactivity of novel chemical and biological entities. We combine scientific rigor with our domain expertise in laboratory automation, informatics, and industry best practices to turn challenging assays into quantitative, reproducible results.

Recent Publications

  • Fan F, Hu R, Munzli A, Chen Y, Dunn RT 2nd, Weikl K, Strauch S, Schwandner R, Afshari CA, Hamadeh H, Nioi P. Utilization of human nuclear receptors as an early counter screen for off-target activity: a case study with a compendium of 615 known drugs. Toxicol Sci. 2015 Jun;145(2):283-95. doi: 10.1093/toxsci/kfv052. Epub 2015 Mar 9
  • Xiong Y, Swaminath G, Cao Q, Yang L, Guo Q, Salomonis H, Lu J, Houze JB, Dransfield PJ, Wang Y, Liu JJ, Wong S, Schwandner R, Steger F, Baribault H, Liu L, Coberly S, Miao L, Zhang J, Lin DC, Schwarz M. Activation of FFA1 mediates GLP-1 secretion in mice. Evidence for allosterism at FFA1. Mol Cell Endocrinol. 2013 Apr 30;369(1-2):119-29. doi: 10.1016/j.mce.2013.01.009. Epub 2013 Feb 9.
  • Yu V, Fisch T, Long AM, Tang J, Lee JH, Hierl M, Chen H, Yakowec P, Schwandner R, Emkey R. High-throughput TR-FRET assays for identifying inhibitors of LSD1 and JMJD2C histone lysine demethylases. 2012. J Biomol Screen. Jan;17(1):27-38.
  • Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I. STK33 kinase activity is nonessential in KRAS-dependent cancer cells. 2011. Cancer Res. Sep 1;71(17):5818-26.
  • Lee T., Schwandner R., Swaminath G., Weiszmann J., Cardozo M., Greenberg J., Jaeckel P., Ge H., Wang Y., Jiao X., Liu J., Kayser F., Tian H., Li Y. 2008. Identification and functional characterization of allosteric agonists for the G protein-coupled receptor FFA2. Mol Pharmacol. Dec;74(6):1599-609.
  • Beck HP., Kohn T., Rubenstein S., Hedberg C., Schwandner R., Hasslinger K., Dai K., Li C., Liang L., Wesche H., Frank B., An S., Wickramasinghe D., Jaen J., Medina J., Hungate R., Shen W. Discovery of potent LPA2 (EDG4) antagonists as potential anticancer agents. 2008. Bioorg Med Chem Lett. Feb 1;18(3):1037-41.
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Diversity Certificates

Small business

Our Services (23)


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Cell Line Generation

Price on request

Mammalian cell line engineering and stable cell line generation

  • High-throughput functional cell clone selection for superior performance in the actual functional (HTS) Assay. Successful applied for several difficult to express membrane targets.

  • Flow cytometry-based generation of cell clones tailored for optimal surface... Show more »

Mammalian cell line engineering and stable cell line generation

  • High-throughput functional cell clone selection for superior performance in the actual functional (HTS) Assay. Successful applied for several difficult to express membrane targets.

  • Flow cytometry-based generation of cell clones tailored for optimal surface expression.

  • Ion channel cell line generation with electrophysiology validation by our partner Metrion Biosciences.

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BD FACSAria Cell Sorting System Cybio FeliX TAP SelecT Robotics

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Assay Development

Price on request

Assay development and optimization for biochemical and cell-based assay formats
- De novo design of predictive and quantitative bioactivity assay. Adaption, miniaturization and automation of client-provided assay protocols. Experience spanning major target classes (GPCRs, Phosphotransferase, Ion Channels, Misc. Enzymes,... Show more »

Assay development and optimization for biochemical and cell-based assay formats
- De novo design of predictive and quantitative bioactivity assay. Adaption, miniaturization and automation of client-provided assay protocols. Experience spanning major target classes (GPCRs, Phosphotransferase, Ion Channels, Misc. Enzymes, Transporters, Binding Proteins, Ubiquitin Ligases across indications (Inflammation, Metabolic, Neuroscience, Oncology)).

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Oncology CNS/Neurology Metabolism and Endocrinology Inflammation

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High Throughput Screening (HTS)

Price on request

Hit identification using Assay.Works small molecule diversity set of 150.000 compounds, client-provided libraries or partner libraries. HTS is supported by integrated time- and temperature controlled multi device platform. Hit identification and HTS work flow are based on biopharmaceutical industry standards with a robust set of... Show more »

Hit identification using Assay.Works small molecule diversity set of 150.000 compounds, client-provided libraries or partner libraries. HTS is supported by integrated time- and temperature controlled multi device platform. Hit identification and HTS work flow are based on biopharmaceutical industry standards with a robust set of best practices, skilled staff, sophisticated laboratory and tissue culture automation in place.

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Perkin Elmer Operetta MD FLIPR Tetra Perkin Elmer EnVision Cybio integrated multi device robotics Cybio FeliX Cytomat 6004 Genedata Screener live-cell imaging Show 8 more tags Show less

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RNAi Screening

Price on request

siRNA Screening:

Target identification or pathway validation using Assay.Works 97.000 siRNA sequences targeting the human
druggable genome. Different RNA chemistries and intrinsic redundancy has been built into the collection to statistically identify false hits.

Combination siRNA Screening:

Genetic interaction... Show more »

siRNA Screening:

Target identification or pathway validation using Assay.Works 97.000 siRNA sequences targeting the human
druggable genome. Different RNA chemistries and intrinsic redundancy has been built into the collection to statistically identify false hits.

Combination siRNA Screening:

Genetic interaction and Pharmacological interaction screening using Assay.Works siRNA collection or 4480 small molecules compounds of the phenotypic toolbox collection

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Molecular Devices FLIPR Oncology CNS/Neurology Metabolism and Endocrinology Inflammation

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Hit to Lead and Lead Optimization

Price on request

Invitro bioactivity assessment
- Characterization of screening hits and lead candidates, concentration response experiments to determine potency efficacy, selectivity and mechanism of action. Collection of 120 engineered cell lines covering a broad range of transmembrane targets available for profiling.

Invitro bioactivity assessment
- Characterization of screening hits and lead candidates, concentration response experiments to determine potency efficacy, selectivity and mechanism of action. Collection of 120 engineered cell lines covering a broad range of transmembrane targets available for profiling.

  • Molecular Interaction by Microscale Thermophoresis (MST) of lead compounds provided by our partner 2bind GmbH.
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Tecan Freedom EVO platforms GPCR Transporter Ion Channel Nuclear Hormone Receptor Phosphotransferase Binding Protein Ubiquitin Ligase Show 8 more tags Show less

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Compound Libraries

Price on request

Assay.ready compound plates
- Gain access to Assay.Works unique small compound collections (seed.74.000, sprout.100.000 and grow. 150.000) optimized for lead-likeness and diversity, free of undesirable chemotypes, verified by LC/UV/MS. Nanoliter dispensing is supported by Assay.Works Labcyte Echo 550® workstation.

Assay.ready compound plates
- Gain access to Assay.Works unique small compound collections (seed.74.000, sprout.100.000 and grow. 150.000) optimized for lead-likeness and diversity, free of undesirable chemotypes, verified by LC/UV/MS. Nanoliter dispensing is supported by Assay.Works Labcyte Echo 550® workstation.

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Labcyte Echo 550 integrated Workstation

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Cell and Tissue Culture

Price on request

Automated production of cellular reagents
- Assay.ready cryopreserved cell production, cell banking, subcellular and supernatants supported by TAP SelecT™ Automated Tissue culture system.

  • Preparation of membrane vesicles for SURFE2R electrogenic event reader (label- and radioactive-free analysis of electrogenic transporters and pumps)

Automated production of cellular reagents
- Assay.ready cryopreserved cell production, cell banking, subcellular and supernatants supported by TAP SelecT™ Automated Tissue culture system.

  • Preparation of membrane vesicles for SURFE2R electrogenic event reader (label- and radioactive-free analysis of electrogenic transporters and pumps)
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TAP SelecT Robotics

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Project Management

Price on request

Design and implementation of scalable laboratory processes and workflows.
Research data and information management.
Technology evaluation and case studies.
Safety and compliance management.

Design and implementation of scalable laboratory processes and workflows.
Research data and information management.
Technology evaluation and case studies.
Safety and compliance management.

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Nucleic Acid Services

Price on request
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Biochemistry & Molecular Biology

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RNA Services

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Lead Identification and Validation

Price on request
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Hit Identification

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Cells and Tissues

Price on request
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Biology

Price on request
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Drug Discovery

Price on request
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Drug Discovery & Development

Price on request
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Mammalian Cell Culture

Price on request
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Cell-Based Compound Screening

Price on request
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Compound Profiling

Price on request
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Live Cell Assay Development

Price on request
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Cell Lines

Price on request
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Cell-Based Assays

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Johannes Knop

Owner & COO
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Ralf Schwandner

Owner & CEO
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Assay.Works - Assay Capabilities.pdf

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Assay.Works - Cell Lines.pdf

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Assay.Works - RNAi.pdf

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Assay.Works - Small Molecules.pdf

  • Positive review for Cell Line Generation:

    March 2019

    "They delivered results in a very timely fashion and communicated very well throughout the whole process."

Assay.Works has not received any endorsements.