Assay.Works
About Assay.Works
Assay.Works is a private contract research organization (CRO) occupying a 750 m2 state-of-the-art laboratory located in the Regensburg BioPark, the second largest hub of the Bavarian biotech cluster. Our mission is to accelerate our partner’s research programs by developing and executing predictive and scalable... Show more »
Assay.Works is a private contract research organization (CRO) occupying a 750 m2 state-of-the-art laboratory located in the Regensburg BioPark, the second largest hub of the Bavarian biotech cluster. Our mission is to accelerate our partner’s research programs by developing and executing predictive and scalable assays to quantify the bioactivity of novel chemical and biological entities. We combine scientific rigor with our domain expertise in laboratory automation, informatics, and industry best practices to turn challenging assays into quantitative, reproducible results.
Recent Publications
- Fan F, Hu R, Munzli A, Chen Y, Dunn RT 2nd, Weikl K, Strauch S, Schwandner R, Afshari CA, Hamadeh H, Nioi P. Utilization of human nuclear receptors as an early counter screen for off-target activity: a case study with a compendium of 615 known drugs. Toxicol Sci. 2015 Jun;145(2):283-95. doi: 10.1093/toxsci/kfv052. Epub 2015 Mar 9
- Xiong Y, Swaminath G, Cao Q, Yang L, Guo Q, Salomonis H, Lu J, Houze JB, Dransfield PJ, Wang Y, Liu JJ, Wong S, Schwandner R, Steger F, Baribault H, Liu L, Coberly S, Miao L, Zhang J, Lin DC, Schwarz M. Activation of FFA1 mediates GLP-1 secretion in mice. Evidence for allosterism at FFA1. Mol Cell Endocrinol. 2013 Apr 30;369(1-2):119-29. doi: 10.1016/j.mce.2013.01.009. Epub 2013 Feb 9.
- Yu V, Fisch T, Long AM, Tang J, Lee JH, Hierl M, Chen H, Yakowec P, Schwandner R, Emkey R. High-throughput TR-FRET assays for identifying inhibitors of LSD1 and JMJD2C histone lysine demethylases. 2012. J Biomol Screen. Jan;17(1):27-38.
- Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I. STK33 kinase activity is nonessential in KRAS-dependent cancer cells. 2011. Cancer Res. Sep 1;71(17):5818-26.
- Lee T., Schwandner R., Swaminath G., Weiszmann J., Cardozo M., Greenberg J., Jaeckel P., Ge H., Wang Y., Jiao X., Liu J., Kayser F., Tian H., Li Y. 2008. Identification and functional characterization of allosteric agonists for the G protein-coupled receptor FFA2. Mol Pharmacol. Dec;74(6):1599-609.
- Beck HP., Kohn T., Rubenstein S., Hedberg C., Schwandner R., Hasslinger K., Dai K., Li C., Liang L., Wesche H., Frank B., An S., Wickramasinghe D., Jaen J., Medina J., Hungate R., Shen W. Discovery of potent LPA2 (EDG4) antagonists as potential anticancer agents. 2008. Bioorg Med Chem Lett. Feb 1;18(3):1037-41.
Diversity Certificates
Our Services (23)
Cell Line Generation
Mammalian cell line engineering and stable cell line generation
High-throughput functional cell clone selection for superior performance in the actual functional (HTS) Assay. Successful applied for several difficult to express membrane targets.
Flow cytometry-based generation of cell clones tailored for optimal surface... Show more »
Mammalian cell line engineering and stable cell line generation
High-throughput functional cell clone selection for superior performance in the actual functional (HTS) Assay. Successful applied for several difficult to express membrane targets.
Flow cytometry-based generation of cell clones tailored for optimal surface expression.
Ion channel cell line generation with electrophysiology validation by our partner Metrion Biosciences.
Assay Development
Assay development and optimization for biochemical and cell-based assay formats
- De novo design of predictive and quantitative bioactivity assay. Adaption, miniaturization and automation of client-provided assay protocols. Experience spanning major target classes (GPCRs, Phosphotransferase, Ion Channels, Misc. Enzymes,... Show more »
Assay development and optimization for biochemical and cell-based assay formats
- De novo design of predictive and quantitative bioactivity assay. Adaption, miniaturization and automation of client-provided assay protocols. Experience spanning major target classes (GPCRs, Phosphotransferase, Ion Channels, Misc. Enzymes, Transporters, Binding Proteins, Ubiquitin Ligases across indications (Inflammation, Metabolic, Neuroscience, Oncology)).
High Throughput Screening (HTS)
Hit identification using Assay.Works small molecule diversity set of 150.000 compounds, client-provided libraries or partner libraries. HTS is supported by integrated time- and temperature controlled multi device platform. Hit identification and HTS work flow are based on biopharmaceutical industry standards with a robust set of... Show more »
Hit identification using Assay.Works small molecule diversity set of 150.000 compounds, client-provided libraries or partner libraries. HTS is supported by integrated time- and temperature controlled multi device platform. Hit identification and HTS work flow are based on biopharmaceutical industry standards with a robust set of best practices, skilled staff, sophisticated laboratory and tissue culture automation in place.
RNAi Screening
siRNA Screening:
Target identification or pathway validation using Assay.Works 97.000 siRNA sequences targeting the human
druggable genome. Different RNA chemistries and intrinsic redundancy has been built into the collection to statistically identify false hits.
Combination siRNA Screening:
Genetic interaction... Show more »
siRNA Screening:
Target identification or pathway validation using Assay.Works 97.000 siRNA sequences targeting the human
druggable genome. Different RNA chemistries and intrinsic redundancy has been built into the collection to statistically identify false hits.
Combination siRNA Screening:
Genetic interaction and Pharmacological interaction screening using Assay.Works siRNA collection or 4480 small molecules compounds of the phenotypic toolbox collection
Hit to Lead and Lead Optimization
Invitro bioactivity assessment
- Characterization of screening hits and lead candidates, concentration response experiments to determine potency efficacy, selectivity and mechanism of action. Collection of 120 engineered cell lines covering a broad range of transmembrane targets available for profiling.
- Molecular Interaction... Show more »
Invitro bioactivity assessment
- Characterization of screening hits and lead candidates, concentration response experiments to determine potency efficacy, selectivity and mechanism of action. Collection of 120 engineered cell lines covering a broad range of transmembrane targets available for profiling.
- Molecular Interaction by Microscale Thermophoresis (MST) of lead compounds provided by our partner 2bind GmbH.
Compound Libraries
Assay.ready compound plates
- Gain access to Assay.Works unique small compound collections (seed.74.000, sprout.100.000 and grow. 150.000) optimized for lead-likeness and diversity, free of undesirable chemotypes, verified by LC/UV/MS. Nanoliter dispensing is supported by Assay.Works Labcyte Echo 550® workstation.
Assay.ready compound plates
- Gain access to Assay.Works unique small compound collections (seed.74.000, sprout.100.000 and grow. 150.000) optimized for lead-likeness and diversity, free of undesirable chemotypes, verified by LC/UV/MS. Nanoliter dispensing is supported by Assay.Works Labcyte Echo 550® workstation.
Cell and Tissue Culture
Automated production of cellular reagents
- Assay.ready cryopreserved cell production, cell banking, subcellular and supernatants supported by TAP SelecT™ Automated Tissue culture system.
- Preparation of membrane vesicles for SURFE2R electrogenic event reader (label- and radioactive-free analysis of electrogenic transporters and pumps)
Automated production of cellular reagents
- Assay.ready cryopreserved cell production, cell banking, subcellular and supernatants supported by TAP SelecT™ Automated Tissue culture system.
- Preparation of membrane vesicles for SURFE2R electrogenic event reader (label- and radioactive-free analysis of electrogenic transporters and pumps)
Project Management
Design and implementation of scalable laboratory processes and workflows.
Research data and information management.
Technology evaluation and case studies.
Safety and compliance management.
Design and implementation of scalable laboratory processes and workflows.
Research data and information management.
Technology evaluation and case studies.
Safety and compliance management.
Nucleic Acid Services
Biochemistry & Molecular Biology
RNA Services
Lead Identification and Validation
Hit Identification
Cells and Tissues
Biology
Drug Discovery
Drug Discovery & Development
Mammalian Cell Culture
Cell-Based Compound Screening
Compound Profiling
Live Cell Assay Development
Cell Lines
Cell-Based Assays
Not finding what you're looking for?
Get info on this provider's capabilities without requesting a quote.Johannes Knop
Owner & COO
Ralf Schwandner
Owner & CEO
Assay.Works - Assay Capabilities.pdf
Assay.Works - Cell Lines.pdf
Assay.Works - RNAi.pdf
Assay.Works - Small Molecules.pdf
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