ARVYS Proteins, Inc.
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Trumbull, Connecticut, US
ARVYS Proteins Inc., a contract research organization (CRO), specializes in custom Protein Services for drug discovery and life science research. Our protein reagents are used in target validation studies, high-throughput assay screening, structural biology research, protein-protein interaction studies, diagnostics and biologics development, in vivo and toxicology studies. Strong integrated expertise in protein biochemistry and cell culture, working experience with various recombinant expression systems and protein classes, up-to-date knowledge in protein technologies, enable us to become a reliable and resourceful partner for our clients.
To assure efficient and economical production of active recombinant proteins, we offer a number of expression systems designed to meet specific goals, ranging from expression of tagged proteins for discovery research to production of biologics for preclinical development. Protein production yields are optimized by selection of an appropriate expression system, generation of a high-producing expression construct, optimization of fermentation/cell culture conditions and purification processes. We also provide native protein purification development and production from natural sources. Our project deliverables are tailored to clients's specifications of scale, purity, analytical characterization and activity.
Labeled proteins allow us to study specific molecular interactions with high sensitivity in complex biological systems. They are important reagents in numerous biological applications such as assays, purifications, protein arrays, localization studies, flow cytometry, clinical imaging and much more. The quality of labeled proteins is critical for consistent and reliable data. Although labeling procedures appear to be simple and straightforward, most of them still need to be adjusted to take into consideration the nature of a protein in order to achieve the desired results. Possible problems during labeling procedures include protein losses due to precipitation, sample manipulation & instability, inconsistent label-to-protein ratio, incomplete removal of an unconjugated labeling probe, and poor protein characterization before and after labeling. In addition, new labeling technologies had emerged for site-directed labeling requiring an integration of protein expression and purification into the labeling process. We have extensive experience with various protein labeling techniques and are confident that we can provide you with high-quality labeled reagents for your downstream applications.
NONSELECTIVE PROTEIN LABELING
SITE-SPECIFIC LABELING
CLICK CHEMISTRY LABELING
BIOMOLECULE IMMOBILIZATION ON SOLID SUPPORT
Endotoxin Removal from biological solutions is critical for many in vivo and cell-based applications, as it interferes with biological response. Endotoxins are liposaccharides that are found in the outer cell wall of Gramm-negative bacteria. Bacteria release endotoxins at the time of lysis. The toxic effect of endotoxins is triggered by its interaction with specific receptors on the immune cells resulting in the release of high concentrations of cytokines and other molecules of immunological significance. Since adventitious endotoxin is present in air, water, labware and it cannot be removed by simple sterilization, it is almost impossible to generate endotoxin-free solutions without a removal procedure. Each Endotoxin Removal project requires a protocol development step that takes into consideration biophysical properties of a target molecule, final sample application and desired formulation. For years we had successfully developed endotoxin removal protocols to meet strict endotoxin presence requirements. We are confident that we can make your Endotoxin Removal project a success.
Custom Endotoxin removal from aqueous solutions of:
Endotoxin removal methods are based on:
Endotoxin removal service features:
Our Protein Expression Services incorporate the latest developments in bacterial, baculovirus and mammalian expression technologies to assure that your recombinant protein production will be efficient and economical. We offer a number of Protein Expression Services designed to meet specific goals, ranging from expression of tagged proteins for research to production of biopharmaceuticals for clinical use. Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host. High level of protein expression also depends on stability, solubility, and folding pathway of the protein product. Accordingly, we will optimize these protein expression parameters when such optimization is necessary.
The quality of starting materials resulting from Fermentation Scale Up often determine the purification strategy or even the success of purification. Our Cell Culture Services aim at achieving maximal r-protein production through optimization of fermentation processes and it could be a very important purification step in itself. Time and effort invested at this stage often result in substantially shorter purification protocols and higher yields of active r-protein. Our experience with various high-producing cell lines, cell culture growth modes, media formulations, methods of protein extraction, fractionation and enrichment enables us to design the most optimal production process for your starting materials.
Large scale plasmid DNA preparation
Large Scale Protein Production
- clarified conditioned media up to 200L
- mammalian cell pellet
- insect cell paste
- bacterial paste from up to 50L of culture
- transient transfection into mammalian cells
- infection of insect cells with high-titer baculovirus
- propagation of stably-expressing insect or mammalian cells
- suspension cell line growth in shake flasks and Wave bioreactors
- adherent cell line growth in multifloor flasks and cell factories.
Our skills in custom protein purification were gained over many years of work with protein pharmaceuticals, drug targets,biological system components and biochemical reagents. All Protein Purification Services start with the analysis of physico-chemical and biological properties of a target protein resulting in the development of tailored procedures for its extraction, purification and characterization. Our purification strategy aims to achieve a homogeneous active protein preparation in two to three purification steps. This goal is reached by a thorough selection and optimization of the capture step, incorporation of a gel filtration step to remove aggregates, degradation products and other contaminants, selection of buffer conditions that stabilize biological activity and prevent product degradation. We routinely work with antibodies,antigens, enzymes, growth factors, DNA-binding proteins, membrane proteins, blood proteins and many more. Some of Although most proteins require an individual approach, we are confident that we can handle your protein. We will purify it cost-efficiently, characterize it according to your specifications and deliver it to you in an active and application-compatible form.
Custom Protein Purification Features
Analysis of intermediate and final purified proteins
All custom purified proteins are
Auxiliary Protein purification services include
Antibodies of different isotypes (including IgMs) will be purified to given specifications from hybridoma or plasma samples. If necessary, endotoxins will be removed to in vivo applications.
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