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ARVYS Proteins, Inc.

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Trumbull, Connecticut, US

About ARVYS Proteins, Inc.

Founded: 2005 Type: Privately Held Size: 1-10 employees

ARVYS Proteins Inc., a contract research organization (CRO), specializes in custom Protein Services for drug discovery and life science research. Our protein reagents are used in target validation studies, high-throughput assay screening, structural biology research, protein-protein interaction studies,... Show more »

ARVYS Proteins Inc., a contract research organization (CRO), specializes in custom Protein Services for drug discovery and life science research. Our protein reagents are used in target validation studies, high-throughput assay screening, structural biology research, protein-protein interaction studies, diagnostics and biologics development, in vivo and toxicology studies. Strong integrated expertise in protein biochemistry and cell culture, working experience with various recombinant expression systems and protein classes, up-to-date knowledge in protein technologies, enable us to become a reliable and resourceful partner for our clients.

To assure efficient and economical production of active recombinant proteins, we offer a number of expression systems designed to meet specific goals, ranging from expression of tagged proteins for discovery research to production of biologics for preclinical development. Protein production yields are optimized by selection of an appropriate expression system, generation of a high-producing expression construct, optimization of fermentation/cell culture conditions and purification processes. We also provide native protein purification development and production from natural sources. Our project deliverables are tailored to clients's specificationsof scale, purity, analytical characterization and activity.

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Our Services (70)


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Fusion Proteins

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Isotope Coded Affinity Tagging (ICAT)

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Protein-Protein Interaction Analysis

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Affinity Tag Purification

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Protein Complementation Assay

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Recombinant Protein Expression

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Large Scale Protein Production

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Computational Protein Design

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Site-Directed Mutagenesis

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Cell-Free Protein Expression

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Cell Services

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HT ADME

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Equilibrium Dialysis

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DNA Library Screening

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Protein Aggregation Analysis

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Drug Target Identification

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Epitope Mapping

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Glycosylation Analysis

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Immunostaining

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In vivo Protein Binding Assays

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Custom Antibody Arrays

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Bacterial Protein Expression

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  • Codon optimization for bacterial expression and gene synthesis of DNA insert
  • Generation of bacterial expression construct(s) in a selected or client-specified vector
  • Transformation into an appropriate host and preparation of glycerol stocks
  • Screening studies for the best-expressing protein variant
  • Optimization of... Show more »
  • Codon optimization for bacterial expression and gene synthesis of DNA insert
  • Generation of bacterial expression construct(s) in a selected or client-specified vector
  • Transformation into an appropriate host and preparation of glycerol stocks
  • Screening studies for the best-expressing protein variant
  • Optimization of growth conditions (host, induction, media, temperature, additives) to drive either soluble or inclusion bodies expression
  • Soluble vs. inclusion bodies expression assessment by SDS-PAGE/Coomassie or Western blot
  • Bacterial paste scale up production
  • Inclusion bodies wash and recovery
  • Recombinant Protein purification from soluble lysate fraction
  • Recombinant Protein purification from inclusion bodies by refolding
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Antibody Products

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Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
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  • Codon optimization for mammalian cell expression and gene synthesis of DNA insert
  • Generation of mammalian protein expression construct(s) in a selected or client-specified vector
  • Endotoxin-free plasmid DNA preparation for transient or stable transfections
  • Small-scale trial to assess recombinant protein expression and/or... Show more »
  • Codon optimization for mammalian cell expression and gene synthesis of DNA insert
  • Generation of mammalian protein expression construct(s) in a selected or client-specified vector
  • Endotoxin-free plasmid DNA preparation for transient or stable transfections
  • Small-scale trial to assess recombinant protein expression and/or to optimize transient transfection conditions
  • Various transient transfection reagent options
  • SDS-PAGE, Western, ELISA or functional expression analysis of conditioned media and/or cell lysates
  • Secreted or intracellular expression in CHO, HEK293, HEK293E or client-specified cells
  • Adherent or suspension cell growth options
  • Large-scale mammalian cell culture for conditioned media or cell pellet production
  • Recombinant protein purification from conditioned media or cell pellet
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Protein Engineering/Directed Evolution

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Baculovirus/Insect Cell Protein Expression

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  • Codon optimization for insect cell expression and gene synthesis of DNA insert
  • Subcloning of a DNA insert into a selected or client-specified baculovirus expression vector
  • Virus generation, amplification and cloning by limited dilution or plaque purification
  • Generation of high-titer virus stocks
  • SDS-PAGE, Western,... Show more »
  • Codon optimization for insect cell expression and gene synthesis of DNA insert
  • Subcloning of a DNA insert into a selected or client-specified baculovirus expression vector
  • Virus generation, amplification and cloning by limited dilution or plaque purification
  • Generation of high-titer virus stocks
  • SDS-PAGE, Western, ELISA or functional expression analysis of cell lysates and conditioned media for intracellular and secreted protein products
  • Secreted or intracellular expression in SF9, SF21 and High Five insect cells
  • Expression optimization studies: MOI, time course, growth media
  • Large-scale insect cell culture for conditioned media or cell pellet production
  • Recombinant protein purification from conditioned media or cell pellet
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Bioconjugation/Chemical Crosslinking

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Labeled proteins allow us to study specific molecular interactions with high sensitivity in complex biological systems. They are important reagents in numerous biological applications such as assays, purifications, protein arrays, localization studies, flow cytometry, clinical imaging and much more. The quality of labeled proteins... Show more »

Labeled proteins allow us to study specific molecular interactions with high sensitivity in complex biological systems. They are important reagents in numerous biological applications such as assays, purifications, protein arrays, localization studies, flow cytometry, clinical imaging and much more. The quality of labeled proteins is critical for consistent and reliable data. Although labeling procedures appear to be simple and straightforward, most of them still need to be adjusted to take into consideration the nature of a protein in order to achieve the desired results. Possible problems during labeling procedures include protein losses due to precipitation, sample manipulation & instability, inconsistent label-to-protein ratio, incomplete removal of an unconjugated labeling probe, and poor protein characterization before and after labeling. In addition, new labeling technologies had emerged for site-directed labeling requiring an integration of protein expression and purification into the labeling process. We have extensive experience with various protein labeling techniques and are confident that we can provide you with high-quality labeled reagents for your downstream applications.

NONSELECTIVE PROTEIN LABELING

  • biotinylation with incorporation ratios determined by a HABA-based assay
  • fluorescent probe conjugation with incorporation ratios determined by fluorescence
  • other chemical moieties (for example, Sulfo-Tag, Dyes) with incorporation ratios determined by UV-Vis spectroscopy
  • enzyme conjugation with incorporation ratios determined by enzyme activity assays

SITE-SPECIFIC LABELING

  • C-terminal labeling through "Sortagging"
  • N-terminal labeling through "Sortagging"
  • labeling at glycosylation sites

CLICK CHEMISTRY LABELING

  • azido modified proteins and/or glycochains
  • alkyne modified proteins and/or glycochains
  • copper (I)-catalyzed click labeling
  • copper-free click labeling
  • various commercial choices of click partners including dual labels
  • in situ applications
  • protein conjugation and detection

BIOMOLECULE IMMOBILIZATION ON SOLID SUPPORT

  • site-specific immobilization on a chip in oriented fashion
  • protein conjugation to chromatography resin
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Accelerated Stability Testing

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Endotoxin Testing

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Endotoxin Removal from biological solutions is critical for many in vivo and cell-based applications, as it interferes with biological response. Endotoxins are liposaccharides that are found in the outer cell wall of Gramm-negative bacteria. Bacteria release endotoxins at the time of lysis. The toxic effect of endotoxins is... Show more »

Endotoxin Removal from biological solutions is critical for many in vivo and cell-based applications, as it interferes with biological response. Endotoxins are liposaccharides that are found in the outer cell wall of Gramm-negative bacteria. Bacteria release endotoxins at the time of lysis. The toxic effect of endotoxins is triggered by its interaction with specific receptors on the immune cells resulting in the release of high concentrations of cytokines and other molecules of immunological significance. Since adventitious endotoxin is present in air, water, labware and it cannot be removed by simple sterilization, it is almost impossible to generate endotoxin-free solutions without a removal procedure. Each Endotoxin Removal project requires a protocol development step that takes into consideration biophysical properties of a target molecule, final sample application and desired formulation. For years we had successfully developed endotoxin removal protocols to meet strict endotoxin presence requirements. We are confident that we can make your Endotoxin Removal project a success.

Custom Endotoxin removal from aqueous solutions of:

  • proteins
  • DNA
  • peptides
  • biomass

Endotoxin removal methods are based on:

  • charge
  • hydrophobicity
  • combinantion of charge and hydrophobicity
  • ligand affinity
  • size

Endotoxin removal service features:

  • protein losses are minimized by selection of an appropriate endotoxin removal method and its optimization
  • scales range from mg to g of a target molecule
  • endotoxin-free samples are sterilized and distributed into multiple aliquots for convenience
  • delivered in an application-compatible buffer
  • turnaround times are less than 1 week in most cases
  • high-throughput assay capacity
  • detergent-compatible r-Factor C fluorescence-based assay is used for Endotoxin Testing in conjunction with Endotoxin Removal service
  • detection levels below 0.01EU/ml
  • LAL kinetic assay and other endotoxin assays are available
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Protein Expression Screening and Optimization

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Our Protein Expression Services incorporate the latest developments in bacterial, baculovirus and mammalian expression technologies to assure that your recombinant protein production will be efficient and economical. We offer a number of Protein Expression Services designed to meet specific goals, ranging from expression of tagged... Show more »

Our Protein Expression Services incorporate the latest developments in bacterial, baculovirus and mammalian expression technologies to assure that your recombinant protein production will be efficient and economical. We offer a number of Protein Expression Services designed to meet specific goals, ranging from expression of tagged proteins for research to production of biopharmaceuticals for clinical use. Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host. High level of protein expression also depends on stability, solubility, and folding pathway of the protein product. Accordingly, we will optimize these protein expression parameters when such optimization is necessary.

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Cell Culture Scale-Up

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The quality of starting materials resulting from Fermentation Scale Up often determine the purification strategy or even the success of purification. Our Cell Culture Services aim at achieving maximal r-protein production through optimization of fermentation processes and it could be a very important purification step in itself.... Show more »

The quality of starting materials resulting from Fermentation Scale Up often determine the purification strategy or even the success of purification. Our Cell Culture Services aim at achieving maximal r-protein production through optimization of fermentation processes and it could be a very important purification step in itself. Time and effort invested at this stage often result in substantially shorter purification protocols and higher yields of active r-protein. Our experience with various high-producing cell lines, cell culture growth modes, media formulations, methods of protein extraction, fractionation and enrichment enables us to design the most optimal production process for your starting materials.

Large scale plasmid DNA preparation
Large Scale Protein Production
- clarified conditioned media up to 200L
- mammalian cell pellet
- insect cell paste
- bacterial paste from up to 50L of culture
- transient transfection into mammalian cells
- infection of insect cells with high-titer baculovirus
- propagation of stably-expressing insect or mammalian cells
- suspension cell line growth in shake flasks and Wave bioreactors
- adherent cell line growth in multifloor flasks and cell factories.

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Protein Purification

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Our skills in custom protein purification were gained over many years of work with protein pharmaceuticals, drug targets,biological system components and biochemical reagents. All Protein Purification Services start with the analysis of physico-chemical and biological properties of a target protein resulting in the development of... Show more »

Our skills in custom protein purification were gained over many years of work with protein pharmaceuticals, drug targets,biological system components and biochemical reagents. All Protein Purification Services start with the analysis of physico-chemical and biological properties of a target protein resulting in the development of tailored procedures for its extraction, purification and characterization. Our purification strategy aims to achieve a homogeneous active protein preparation in two to three purification steps. This goal is reached by a thorough selection and optimization of the capture step, incorporation of a gel filtration step to remove aggregates, degradation products and other contaminants, selection of buffer conditions that stabilize biological activity and prevent product degradation. We routinely work with antibodies,antigens, enzymes, growth factors, DNA-binding proteins, membrane proteins, blood proteins and many more. Some of Although most proteins require an individual approach, we are confident that we can handle your protein. We will purify it cost-efficiently, characterize it according to your specifications and deliver it to you in an active and application-compatible form.

Custom Protein Purification Features

  • purification of
    • fusion and tagged proteins from recombinant sources
    • native proteins from natural sources
    • antibodies of different isotypes including IgMs
    • membrane proteins
  • common protein purification scales range from 0.001g to 5g
  • proteins are purified according to client-specified purity
  • Protein purification methods from
    • client-supplied protocols
    • published protocols
    • improved protocols from client-supplied or published protocols
    • de novo protocols tailored to client's requirements
  • any protein purification mode can be used
    • ion-exchange
    • gel filtration
    • affinity (broad-spectrum)
    • hydrophobic interaction
  • refolding from inclusion bodies
  • protein purification method development for transfer to a GMP facility
  • dedicated columns are used in each project
  • efficiency is provided by the automation and precision of AKTA systems from Amersham BioSciences (currently GE)

Analysis of intermediate and final purified proteins

  • SDS-PAGE and/or dot/Western blotting are routinely used for fraction analysis
  • final protein purity is determined by densitometry from Coomassie-stained gels
  • final products are supplied with a certificate of analysis,purification report and, if applicable, a batch record

All custom purified proteins are

  • supplied with a certificate of analysis tailored to client's specification
  • provided at specified protein concentration
  • formulated in a buffer that protects from protein degradation due to proteolysis, oxidation and shear stress
  • dispensed into specified aliquot sizes

Auxiliary Protein purification services include

  • protein refolding
  • endotoxin removal
  • tag removal
  • labeling and conjugation
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Custom Monoclonal Antibody (mAb) Production

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ELISA

Enzyme-linked immunosorbent assay
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Protein Refolding

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Western Blot

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Biochemical Assay Kits

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Antibody Purification

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Antibodies of different isotypes (including IgMs) will be purified to given specifications from hybridoma or plasma samples. If necessary, endotoxins will be removed to in vivo applications.

Antibodies of different isotypes (including IgMs) will be purified to given specifications from hybridoma or plasma samples. If necessary, endotoxins will be removed to in vivo applications.

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Lyophilization

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HPLC

High Performance Liquid Chromatography
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Assay Development

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Biology

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Pharmacology & Toxicology

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Protein Production

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Liquid Chromatography (LC)

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Cell and Tissue Culture

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Protein Quantification

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Cells and Tissues

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Antibody Services

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Nucleic Acid Services

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Drug Discovery

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DNA Services

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Protein Services

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Mutagenesis

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Chemistry and Materials

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Protein Expression Visualization

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ADME/DMPK Studies

Drug Metabolism and Pharmacokinetics
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Drug Discovery & Development

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Analytical Chemistry Services

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Product Development, Testing, and Packaging

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Biomolecular Interaction Analysis

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Biochemical Assays

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Mammalian Cell Culture

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Formulation Services

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Biochemistry & Molecular Biology

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Computational Modeling

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Lead Identification and Validation

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Project Management

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Chromatography

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Protein Characterization

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