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Applied StemCell, Inc.

Milpitas, California, US

Applied StemCell Inc. (ASC) is a fast-growing biotechnology company providing custom animal and cell line models as tools for drug discovery and diagnostics. Our gene editing platforms include CRISPR/Cas9 and our proprietary TARGATT™ integrase technology for site-specific, efficient knock-in of transgenes up to 20 kb in size. Our company employs these platforms to enable scientific advances ranging from pre-clinical discovery to clinical application.

Custom Gene Editing in Cell Line and Animal Models
ASC uses CRISPR/Cas9 and TARGATT technologies to enable a large number of custom genetic modifications including knock-in, knock-out, point mutations, and large fragment knock-in. These modifications can be made for several pre-clinical applications, including:

  • Mouse models
  • Rat models
  • iPSC models (normal and patient-derived)
  • Cell line models

**Diagnostic Reference Standards and Custom FFPE... Show more »

Applied StemCell Inc. (ASC) is a fast-growing biotechnology company providing custom animal and cell line models as tools for drug discovery and diagnostics. Our gene editing platforms include CRISPR/Cas9 and our proprietary TARGATT™ integrase technology for site-specific, efficient knock-in of transgenes up to 20 kb in size. Our company employs these platforms to enable scientific advances ranging from pre-clinical discovery to clinical application.

Custom Gene Editing in Cell Line and Animal Models
ASC uses CRISPR/Cas9 and TARGATT technologies to enable a large number of custom genetic modifications including knock-in, knock-out, point mutations, and large fragment knock-in. These modifications can be made for several pre-clinical applications, including:

  • Mouse models
  • Rat models
  • iPSC models (normal and patient-derived)
  • Cell line models

Diagnostic Reference Standards and Custom FFPE Products
ASC offers isogenically-paired mutant and wild-type cell lines as FFPE reference standards for assay development. In addition, we offer a number of custom FFPE cell line services, which enables us to incorporate your own cell lines into FFPE blocks, slides, or scrolls.

Other Products and Services
ASC offers a number of other products and services including iPSC differentiation and characterization, as well as culture reagents and pre-validated gene editing kits.

Applied StemCell Inc. is AAALAC, ISO 9001, and ISO 13485 certified.

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Applied StemCell, Inc. has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
Genome Editing
Price on request
Request a quote for more information about this service.
CRISPR/Cas9 Genome Editing
Price on request
Request a quote for more information about this service.
Neurotoxicity Testing
Starting at $100.00 per test

We provide stage-specific phenotype assays in our neurotoxicity/ drug screening platform:
Dose-response curve for neurotoxicity and drug efficacy
Screening in multiple cell types: iPSCs, NSCs, neural cells
Cellular Morphology
Synaptogenesis, network formation using neuronal co-cultures

Cytotoxicity &... Show more »

We provide stage-specific phenotype assays in our neurotoxicity/ drug screening platform:
Dose-response curve for neurotoxicity and drug efficacy
Screening in multiple cell types: iPSCs, NSCs, neural cells
Cellular Morphology
Synaptogenesis, network formation using neuronal co-cultures

Cytotoxicity & Cell Viability Assays
MTT/ MTS cell proliferation assay
LDH, Necrosis and Apoptosis assays
Luciferase (bioluminescence) expression
cAMP level measurement

Mitochondrial Toxicity Testing
Volume fraction detection

Functional Assays
Calcium influx/ imaging
Electrophysiology: Multielectrode array (MEA) analysis and Patch clamp recording

Quantitative Gene Expression
qPCR
RNA-seq using NGS (next generation sequencing)

Morphology
Neurite growth assay
Biomarker screening

Cell-based in vitro assays of drug toxicity are becoming crucial tools to screen new drug candidates before moving to expensive animal models testing. They are inexpensive, efficient, and ethically compatible preliminary screening alternatives to using animal models. Human iPSC-derived neuronal cells have been shown to be more physiologically relevant neuronal models than immortalized cell lines, can improve drug discovery and safety assessment, and reduce late stage drug attrition by providing a reliable drug testing platform.
Applied StemCell (ASC) offers a one-stop-solution for neurological compound screening: generating iPSCs (healthy and diseased patient samples), disease modeling via gene editing for drug target discovery and efficacy testing, differentiation to cell lineage(s) of choice, cell line model characterization, as well as, a comprehensive cell-based test battery for drug efficacy, neurotoxicity and target discovery that are regulatory compliant.

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Human
Mouse
Regenerative medicine
neurological
neurology
CNS/Neurology
neuroscience
Neuroscience
neurodegenerative disorders
neurodegenerative diseases
neuropathy
Central Nervous System
Neurodegenerative
Knockout Mouse Development
Price on request

Knock-out/in Mice
We provide high quality service for generation of knock-out/in mice models.

Our Services:
• Gene targeting vector construction
• ES cell targeting
• Cre/FLP recombination
• Chimera production by blastocyst injection

Cost-effective:
Applied StemCell has years of experience and a high success rate in... Show more »

Knock-out/in Mice
We provide high quality service for generation of knock-out/in mice models.

Our Services:
• Gene targeting vector construction
• ES cell targeting
• Cre/FLP recombination
• Chimera production by blastocyst injection

Cost-effective:
Applied StemCell has years of experience and a high success rate in generating conventional gene-targeted mice with our proprietary mESC lines.

High-Quality:
Our scientists have extensive experience in gene targeting and genetic mouse models. All mice are generated and shipped from our California facility (NIH guidance certified).

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Mouse
CRISPR
Knock-in Mouse Development
Price on request

Site-Specific!
Applied Stem Cell Inc.’s proprietary TARGATT™ Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active... Show more »

Site-Specific!
Applied Stem Cell Inc.’s proprietary TARGATT™ Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Applied StemCell can create site-specific knock-in mice for you in as fast as 3 months. Using our novel TARGATT™ system, a gene of interest can be inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and/or ubiquitous expression options are available. The TARGATT™-mice are available in 2 backgrounds: FVB and C57BL6J). Please contact us to discuss your project plan.

Advantages of TARGATT™ Technology

  1. High integration efficiency mediated by PhiC31 integrase reduces time and cost
  2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene
  3. Integration at intergenic region ensures that no internal genes are interrupted
  4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability
  5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

TARGATT™ Technology can be utilized for a variety of applications including reporter gene expression, gene knockdown and disease models.

We supply 2 strains of

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Mouse
Genetically Engineered Mice
Price on request

Transgenic mice using CRISPR/Cas9 system:
Applied StemCell offers CRISPR/Cas9 or Homologous recombination mediated engineering of custom transgenic mice. Our scientific experts offer many types of gene modification, including knock-out, knock-in, gene replacement, point mutation, precise deletion, and conditional knock-out... Show more »

Transgenic mice using CRISPR/Cas9 system:
Applied StemCell offers CRISPR/Cas9 or Homologous recombination mediated engineering of custom transgenic mice. Our scientific experts offer many types of gene modification, including knock-out, knock-in, gene replacement, point mutation, precise deletion, and conditional knock-out services. Interested in other variants? Our scientists will help you evaluate the feasibility of your transgenic model. We offer engineering on a C57BL6 background, or on your strain of interest.

Traditional Transgenic Mice: Homologous Recombination:
Applied StemCell offers Cas9-mediated engineering of custom transgenic mice. Our scientific experts offer many types of gene modification, including knock-out, knock-in, gene replacement, point mutation, precise deletion, and conditional knock-out services. Interested in other variants? Our scientists will help you evaluate the feasibility of your transgenic model. We offer engineering on a C57BL6 background, or on your strain of interest.

Applied StemCell, Inc. offers various services related to animal models including conventional transgenic mice, transgenic rats, and phenotype analysis. Applied StemCell, Inc. has an AAALAC accredited animal facility and all mice and rats are generated within the USA.

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Mouse
Rat
CRISPR Knock-out Cell Lines
Price on request

Custom iPSC Engineering Services – 4 Reasons to Choose Applied StemCell! We are the most experienced company in the industry!
iPSC genome editing is a highly technical process and one that is best handled by a company with specialized expertise, validated protocols, patented technology, and a track record of success. There are... Show more »

Custom iPSC Engineering Services – 4 Reasons to Choose Applied StemCell! We are the most experienced company in the industry!
iPSC genome editing is a highly technical process and one that is best handled by a company with specialized expertise, validated protocols, patented technology, and a track record of success. There are four reasons why Applied StemCell’s iPSC Genome Engineering Service matches this description and is unique within the genome editing marketplace.
1. ASC Has Extensive Experience in Stem Cell and Genome Editing Technologies
ASC licensed rights to Yamanaka reprogramming factors from iPS Academia Japan, and also has the rights to use iPSCs as starting material for gene editing and differentiation. ASC is also a premier licensee of the CRISPR/Cas9 technology from the Broad Institute.
2. Provides iPSC Genome Editing Services for Cell Lines from Healthy and Diseased Donors
In addition to providing expertise in stem cell and genome editing technologies, ASC can provide iPSC genome editing services for cell lines from healthy and diseased donors. Importantly, ASC has a well-characterized human iPSC cell line (with teratoma formation analysis) that was reprogrammed from fibroblasts of a healthy donor and is very responsive to CRISPR-based genome editing.
3. Applied StemCell’s Service Portfolio Accommodates Fully Customizable Deliverables for Customers
Additionally, ASC’s service portfolio accommodates fully customizable deliverables for customers, a common limitation among companies that have less experience with iPSC genome engineering.
4. Quality of Client Experience
Finally, Applied StemCell has extensive experience in CRISPR Genome Editing with iPSCs. It has executed custom projects for well-known clients. It operates with professional project managers. It provides high-resolution, publication-quality images.
Please ask us for details of services.

Applied StemCell offers Cas9-mediated cell line engineering. Our scientific experts have successfully engineered and delivered many types of gene modificationin a wide variety of cell lines most notably in HEMATOPOIETIC CELLS (TF-1 and Jurkat)

Service includes:
- knock-out
- knock-in
- gene replacement
- point mutation
- precise deletion
- conditional knock-out
- Disease Models
- Reporter Lines

Service includes: Targeting vector construction and confirmation, Cell transfection and selection, Screening gene-edited cell clones, and Expansion and cryopreservation of engineered cells.

Applied Stem Cell also offers CRISPR/Cas9 engineered cell line disease models. You provide us with either your source cells or iPSCs, or we can start with our own iPSCs. Applied StemCell will genetically modify the iPSCs according to your specifications using CRISPR/ Cas9.

Source cells can be fibroblasts, PMBCs, or other biosamples. Applied StemCell has optimized transfection and culture conditions for iPSCs that provide the healthiest and most robust iPSCs on the market, genetically modified to your specifications.

  • Generate iPSCs (healthy / disease)
  • Knock-out, knock-in your gene of interest (bio marker)
  • Introduce or correct mutations
  • Conditional knock-out of genes
  • Further differentiation to certain progenitor cells to characterize/compare phenotype

Deliverables:
- At least 1 clone (with 2 vials of >2x10^5 cell/vial per clone)

With strong expertise in genomic engineering and cell culture techniques, Applied StemCell can make your customized genetically modified cell lines. We specialize in both adherent and suspension cell culture. With a great variety of tools for cell line gene modification we have successfully made genetically modified cells for a variety of cell lines including cells that are not susceptible to transfection or clonal selection.

We use CRISPR/Cas9, our proprietary TARGATT™, and homologous recombination technologies to make various forms of gene modification, including knockout, knockin, gene replacement, point mutation, and deletions. TARGATT™ technology enables highly efficient, site-specific gene integration into the mammalian genome with robust gene expression. The technology uses phiC31 integrase to insert any gene of interest into a safe harbor locus in a transcriptionally active, intergenic region.


Turnaround time: 3-9 months
Deliverables: 2 vials, 0.5x10^/6 cells/vial AND full report


Other applications

CRISPR/Cas9 Knock-in Knock-out Rat Model Generation Service:
We use our proprietary TARGATT™, CRISPR/Cas9, and homologous recombination technologies to make various forms of gene modification, including knockout, knockin, gene replacement, point mutation, and deletions.

Updates:
Applied StemCell, Inc. announces licensing agreement with Broad Institute for CRISPR/Cas9 Gene-Editing technology.

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Cell culture
CRISPR
Cell line engineering
CRISPR Knock-In Cell Lines
Price on request

Applied StemCell offers Cas9-mediated cell line engineering. Our scientific experts have successfully engineered and delivered many types of gene modificationin a wide variety of cell lines most notably in HEMATOPOIETIC CELLS (TF-1 and Jurkat)

Service includes:
- knock-out
- knock-in
- gene replacement
- point mutation
-... Show more »

Applied StemCell offers Cas9-mediated cell line engineering. Our scientific experts have successfully engineered and delivered many types of gene modificationin a wide variety of cell lines most notably in HEMATOPOIETIC CELLS (TF-1 and Jurkat)

Service includes:
- knock-out
- knock-in
- gene replacement
- point mutation
- precise deletion
- conditional knock-out
- Disease Models
- Reporter Lines

Service includes: Targeting vector construction and confirmation, Cell transfection and selection, Screening gene-edited cell clones, and Expansion and cryopreservation of engineered cells.

Applied Stem Cell also offers CRISPR/Cas9 engineered cell line disease models. You provide us with either your source cells or iPSCs, or we can start with our own iPSCs. Applied StemCell will genetically modify the iPSCs according to your specifications using CRISPR/ Cas9.

Source cells can be fibroblasts, PMBCs, or other biosamples. Applied StemCell has optimized transfection and culture conditions for iPSCs that provide the healthiest and most robust iPSCs on the market, genetically modified to your specifications.

  • Generate iPSCs (healthy / disease)
  • Knock-out, knock-in your gene of interest (bio marker)
  • Introduce or correct mutations
  • Conditional knock-out of genes
  • Further differentiation to certain progenitor cells to characterize/compare phenotype

Deliverables:
- At least 1 clone (with 2 vials of >2x10^5 cell/vial per clone)

With strong expertise in genomic engineering and cell culture techniques, Applied StemCell can make your customized genetically modified cell lines. We specialize in both adherent and suspension cell culture. With a great variety of tools for cell line gene modification we have successfully made genetically modified cells for a variety of cell lines including cells that are not susceptible to transfection or clonal selection.

We use CRISPR/Cas9, our proprietary TARGATT™, and homologous recombination technologies to make various forms of gene modification, including knockout, knockin, gene replacement, point mutation, and deletions. TARGATT™ technology enables highly efficient, site-specific gene integration into the mammalian genome with robust gene expression. The technology uses phiC31 integrase to insert any gene of interest into a safe harbor locus in a transcriptionally active, intergenic region.


Turnaround time: 3-9 months
Deliverables: 2 vials, 0.5x10^/6 cells/vial AND full report


Other applications

CRISPR/Cas9 Knock-in Knock-out Rat Model Generation Service:
We use our proprietary TARGATT™, CRISPR/Cas9, and homologous recombination technologies to make various forms of gene modification, including knockout, knockin, gene replacement, point mutation, and deletions.

Updates:
Applied StemCell, Inc. announces licensing agreement with Broad Institute for CRISPR/Cas9 Gene-Editing technology.

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Human
Mouse
Rat
Stable Cell Line Generation
Price on request

Possible Cell Line Engineering Projects (selected):

Knock-in/knock-out cell lines
Gene replacement
Gene editing including single base mutations
Gene tagging
Gene Therapy
Promoter modifications
Mutagenesis
Removal of viral sequences
Stable cell lines / immortalizatioin

CRISPR/Cas9, Lentivirus, and other methods are available.

Possible Cell Line Engineering Projects (selected):

Knock-in/knock-out cell lines
Gene replacement
Gene editing including single base mutations
Gene tagging
Gene Therapy
Promoter modifications
Mutagenesis
Removal of viral sequences
Stable cell lines / immortalizatioin

CRISPR/Cas9, Lentivirus, and other methods are available.

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Human
Mouse
Cell culture
CRISPR
Cell line engineering
lentivirus
Non-human primates
CRISPR-Mediated Targeted Gene Integration
Price on request

Custom iPSC Engineering Services – 4 Reasons to Choose Applied StemCell! We are the most experienced company in the industry!
iPSC genome editing is a highly technical process and one that is best handled by a company with specialized expertise, validated protocols, patented technology, and a track record of success. There are... Show more »

Custom iPSC Engineering Services – 4 Reasons to Choose Applied StemCell! We are the most experienced company in the industry!
iPSC genome editing is a highly technical process and one that is best handled by a company with specialized expertise, validated protocols, patented technology, and a track record of success. There are four reasons why Applied StemCell’s iPSC Genome Engineering Service matches this description and is unique within the genome editing marketplace.
1. ASC Has Extensive Experience in Stem Cell and Genome Editing Technologies
ASC licensed rights to Yamanaka reprogramming factors from iPS Academia Japan, and also has the rights to use iPSCs as starting material for gene editing and differentiation. ASC is also a premier licensee of the CRISPR/Cas9 technology from the Broad Institute.
2. Provides iPSC Genome Editing Services for Cell Lines from Healthy and Diseased Donors
In addition to providing expertise in stem cell and genome editing technologies, ASC can provide iPSC genome editing services for cell lines from healthy and diseased donors. Importantly, ASC has a well-characterized human iPSC cell line (with teratoma formation analysis) that was reprogrammed from fibroblasts of a healthy donor and is very responsive to CRISPR-based genome editing.
3. Applied StemCell’s Service Portfolio Accommodates Fully Customizable Deliverables for Customers
Additionally, ASC’s service portfolio accommodates fully customizable deliverables for customers, a common limitation among companies that have less experience with iPSC genome engineering.
4. Quality of Client Experience
Finally, Applied StemCell has extensive experience in CRISPR Genome Editing with iPSCs. It has executed custom projects for well-known clients. It operates with professional project managers. It provides high-resolution, publication-quality images.
Please ask us for details of services.

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Human
Mouse
Cell culture
iPS cell engineering
Cell line engineering
Non-human primates
TARGATT Genome Editing
Price on request

TARGATT™ Gene Targeting in Cell Lines

Advantages of TARGATT™ Technology:
- Site-specific DNA integration (knock-in) in defined, transcriptionally-active locus

  • Ensures robust gene expression
  • Eliminates unpredictable phenotypes caused by random integration
  • Allows for proper comparison of different transgenes
    -... Show more »

TARGATT™ Gene Targeting in Cell Lines

Advantages of TARGATT™ Technology:
- Site-specific DNA integration (knock-in) in defined, transcriptionally-active locus

  • Ensures robust gene expression
  • Eliminates unpredictable phenotypes caused by random integration
  • Allows for proper comparison of different transgenes
  • Single-copy integration: Eliminates repeat-induced gene silencing and genomic instability.
  • Insertion of intact transgene: Avoids partial transgenes with unexpected phenotype

  • High integration efficiency

  • Save months of time and costs

TARGATT™ Technology in Cell Lines
- TARGATT™ site is placed in the specified locus in the genome of your cell line by homologous recombination
- TARGATT™ site can then be used for gene integration into this locus (for any gene of interest)

We offer a customized TARGATT™ gene targeting service for any cell line for your projects, e.g.

Gene Editing, Gene Modification, Cell Based Assays and Drug Screening (HTP Screening).

Service options:

  1. Vector design and construction

  2. Electroporation (or other transfection) into cell line of choice (suspension or adherent)

  3. Selection of clones and screening for successful integration

  4. Expansion of targeted clones

TARGATT™ Master Human iPSC line

The TARGATT™ master iPSC line contains a "docking attP" site at a safe harbor genomic Rosa26 locus. Any genes of interest on an attB vector can be inserted efficiently at the Rosa26 locus through phiC31 integrase mediated recombination between attP and attB. Efficiency of transgene insertion is up to 100% with drug selection and up to 30% without drug selection.

Application:

iPSC reporter lines for cell tracking
iPSC lines with surface markers for cell purification
iPSC lines with cell-specific promoters to direct iPSC differentiation

ASC provides iPSC services under licensing agreement with iPS Academia, Japan.

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Human
Mouse
Induced Pluripotent Stem Cell (iPSC) Differentiation
Price on request

Applied StemCell Inc. (ASC) offers various iPSC differentiation services for ESC / iPSC differentiation into more specialized cells including multipotent stem cells and fully differentiated somatic cells. Differentiation can be performed using either our in-house iPSC lines, or your own pluripotent cell line.

Applied StemCell Inc. (ASC) offers various iPSC differentiation services for ESC / iPSC differentiation into more specialized cells including multipotent stem cells and fully differentiated somatic cells. Differentiation can be performed using either our in-house iPSC lines, or your own pluripotent cell line.

  • Neural Progenitor Cells (NPCs)
  • Motor Neurons
  • Cardiomyocytes
  • Hepatocytes

Advantage of using ESC / iPSC differentiation to produce multipotent stem cells:
- Thaw ESC/iPSC-derived multipotent stem cells (e.g. Neural Progenitor Cells) when you need them
- Differentiate multipotent stem cells to specialized somatic cells (e.g. Motor Neurons).
- Expand the multipotent stem cells for a ready supply for your planned experiments = save money and time!

ASC provides iPSC services under licensing agreement with iPS Academia, Japan.

Deliverables: 2 vials od 5x10^5 cells/ vial

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Cell culture
Induced Pluripotent Stem Cell (iPSC) Culture
Price on request

We offer standard and customized cell culture services including expansion, plating, mycoplasma testing and FBS lot evaluation.
Please contact us to discuss your needs with our technical service specialists.

We offer standard and customized cell culture services including expansion, plating, mycoplasma testing and FBS lot evaluation.
Please contact us to discuss your needs with our technical service specialists.

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Cell culture
Teratoma Formation
Price on request

All-inclusive Teratoma Formation & Analysis Service for the characterization of pluripotent human and mouse ES/iPS cells. Publication-quality images and a summary report including a list of identified tissues will be provided.

  • Fast turnaround time--as short as four weeks.
  • Only 0.5 million (mouse) or 1-2 million... Show more »

All-inclusive Teratoma Formation & Analysis Service for the characterization of pluripotent human and mouse ES/iPS cells. Publication-quality images and a summary report including a list of identified tissues will be provided.

  • Fast turnaround time--as short as four weeks.
  • Only 0.5 million (mouse) or 1-2 million (human) cells per injection site
  • We perform both kidney and testis injections with a >90% success rate of teratoma formation.

What you get from us:
- Detailed report including protocol description and a list of identified tissues with images
- CD with all images in high resolution
- Tissue blocks
- H & E slides
- Snap-frozen teratoma tissue (if requested)

What we need from you:
- Mouse ES/iPS: 3-6 X 10^6 cells
- Human ES/iPS: 20 X 10^6 cells


Teratoma Formation Analysis (for non-Human/Mouse species)

All-inclusive Teratoma Formation & Analysis Service for the characterization of pluripotent ES/iPS cells from non-human, non-mouse species. Publication-quality images and a summary report including a list of identified tissues will be provided.

Please inquire for details.

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Embryoid body formation
Price on request

Embryoid Body (EB) Formation and Characterization Service

We offer a complete embryoid body (EB) formation and characterization service to evaluate the pluripotency and the differentiation potential of mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in vitro.

Service includes:

EB... Show more »

Embryoid Body (EB) Formation and Characterization Service

We offer a complete embryoid body (EB) formation and characterization service to evaluate the pluripotency and the differentiation potential of mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in vitro.

Service includes:

EB formation from human or mouse ESCs or iPSCs
EB embedding, sectioning, and staining
Full report with high-resolution, publication-grade images
Efficiency:

Primary screen for markers representing all three germ layers
Data ready for publication of your new cell line
Turnaround time: 3 weeks

Markers:

Nestin
Beta III tubulin
Desmin
Smooth muscle actin
Alpha fetoprotein
Please contact us if you require different or additional markers.

What you get from us:

Detailed report including protocol description and a list of identified tissues with images
CD with all images in high resolution

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Knockout Rat Development
Price on request

Applied StemCell utilizes Cas9-CRISPR service to generate rat models that contain point mutation(s), small reporter insertions, conditional knockout and knockout.

Applied StemCell utilizes Cas9-CRISPR service to generate rat models that contain point mutation(s), small reporter insertions, conditional knockout and knockout.

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Rat
Knock-in Rat Development
Price on request

Applied StemCell utilizes Cas9-CRISPR, BAC and TARGATT(TM) service to generate rat models that contain reporter insertions and conditional knockout.

Applied StemCell utilizes Cas9-CRISPR, BAC and TARGATT(TM) service to generate rat models that contain reporter insertions and conditional knockout.

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Rat
Transgenic Rat Development
Price on request

Applied StemCell, Inc., offers various services related to animal models including conventional transgenic rat, transgenic rats, and phenotype analysis. Applied StemCell, Inc. has an AAALAC accredited animal facility and all mice and rats are generated within the USA.

TARGATT™ transgenic rats

Our TARGATT™ technology uses... Show more »

Applied StemCell, Inc., offers various services related to animal models including conventional transgenic rat, transgenic rats, and phenotype analysis. Applied StemCell, Inc. has an AAALAC accredited animal facility and all mice and rats are generated within the USA.

TARGATT™ transgenic rats

Our TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site, that has been pre-engineered in an intergenic and transcriptionally active genomic locus with guaranteed transgene expression. Applied StemCell, Inc. can create cost-effective, site-specific knock-in rats for you in as little as 7-9 months. This technology can be utilized for a variety of applications including reporter gene expression, gene knock-down and disease models.

CRISPR/Cas9 knock-in, knock-out, conditional knock-out rats

Cas9-CRISPR method will be used to generate rat models that contain point mutation(s), small reporter insertions and conditional knockouts. The CRISPR/Cas9 system uses the Cas9 nuclease to facilitate RNA-guided site-specific DNA cleavage. The system consists of two components: (1) mammalian codon-optimized version of the Cas9 protein carrying a nuclear localization signal to ensure nuclear compartmentalization in mammalian cells; (2) guide RNAs (gRNAs) to direct Cas9 protein to sequence-specifically cleave the targeted DNA. The advantage of CRISPR/Cas9 over ZFNs or TALENs is its scalability and multiplexibility in that multiple sites within the mammalian genome can be simultaneously modified, providing a robust, high-throughput approach for gene editing in mammalian cells.

Applied StemCell, Inc. can generate CRISPR/Cas9 transgenic rats in as little as 7-9 months.

BACTERIAL ARTIFICIAL CHROMOSOME (BAC)

Bacterial Artificial Chromosomes (BACs) are ultra-low copy vectors that can hold up to 300 kb of genomic fragments, making them ideal vectors for introduction of entire genes including the regulatory regions into the rat genome. BAC transgenic rats are similar to other knock-in models in the expression pattern of the gene of interest, except that the BAC is randomly integrated in the genome as a transgene. Applied StemCell can create a BAC knock-in and conditional knock-in rat models for you.

Our services include:
BAC design and cloning strategy
Cloning and sequence validation of the BAC tailor-made to your specifications
Pronuclear microinjection of BAC into rat embryos
Genotyping

Alternatively, you can choose to have us do the BAC modification strategy and cloning, and you can do the pronuclear injections and transgenic rat screening yourself.

Please contact us so we can help you plan your projects and give in-depth detail on transgenic rat and additional services we offer to streamline your research.

Transgenic Rat Development

Site-specific transgenic rats are gaining importance as a preferred in vivo model for researchers who seek better representation of human genetics and physiology. Their similarity to human physiology and pathology makes them a better in vivo model than mice for the study of cardiovascular, diabetics, neurological, psychiatric and autoimmune dysfunctions.

Using Applied StemCell, Inc's., proprietary TARGATT™ and CRISPR/Cas9 technologies, we can generate site-specific transgenic rats in as little as 7 months. The novel TARGATT™ system uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus.

Advantages of the TARGATT™ system:

High integration efficiency mediated by PhiC31 integrase- reduces time and cost
Site-specific integration at a pre-selected genomic locus - eliminates position effect and ensures high expression levels of the transgene
Integration at intergenic region - ensures that no internal genes are interrupted
Single copy gene integration - eliminates repeat-induced gene silencing and genomic instability
Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.
Please contact us for more details and to explore our other custom rat gene editing services.

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Rat
Custom FFPE Cell Line Slides
Price on request

Custom FFPE Processing of Your Cell Lines: Single or Multi-Spot Capabilities
ASC now provides custom FFPE processing services. Ship us your own cells, and we will culture, fix, and incorporate your cell lines into custom FFPE blocks that can be used to generate custom FFPE slides or scrolls.

Benefits:
• Generation of FFPE... Show more »

Custom FFPE Processing of Your Cell Lines: Single or Multi-Spot Capabilities
ASC now provides custom FFPE processing services. Ship us your own cells, and we will culture, fix, and incorporate your cell lines into custom FFPE blocks that can be used to generate custom FFPE slides or scrolls.

Benefits:
• Generation of FFPE reference materials from your own lab’s cell lines
• Utilize our multi-spotting capacity to mix and match different mutations or cell lines on a single slide
• Perfect for pairing drug discovery with companion diagnostics development

Available FFPE formats include:
• FFPE Slides: Custom FFPE slides are the perfect solution to your IHC and ISH application needs. ASC offers both single-spotting and custom multi-spotting services.
• FFPE Scrolls: Custom FFPE scrolls can be used as reference materials for in vitro diagnostics development or other applications.
• FFPE Blocks: Full FFPE blocks provide you with bulk material for the generation of your own FFPE slides and scrolls

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Human
Cancer
CRISPR/Cas9 vector generation
Price on request

Custom CRISP/Cas9 plasmid design / construction
Let our experts design and generate your vectors for genome editing.

Choose our full service CRISPR/Cas9 vector design, construction and gRNA validation for:

  • Transfection into cell lines
  • Microinjection into embryos for KI/KO/CKO animal models
  • Virus packaging

... Show more »

Custom CRISP/Cas9 plasmid design / construction
Let our experts design and generate your vectors for genome editing.

Choose our full service CRISPR/Cas9 vector design, construction and gRNA validation for:

  • Transfection into cell lines
  • Microinjection into embryos for KI/KO/CKO animal models
  • Virus packaging

Or let Applied StemCell generate your cell line or mouse/rat model for you!
Applied StemCell, Inc. has years of expertise generating genetically modified cell lines (multiple species) and animal models (rats, mice).

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Mouse
Rat
Blastocyst Injection
Price on request

Germline Transmission Service by Blastocyst Injection

Germline transmission testing is the gold standard for pluripotency testing of iPSC lines. Applied StemCell provides germline-transmission testing for mouse ESC/iPSC lines via blastocyst injection. Chimeric mice are generated and bred to determine germline transmission.

We proide full report of genotyping results.

Germline Transmission Service by Blastocyst Injection

Germline transmission testing is the gold standard for pluripotency testing of iPSC lines. Applied StemCell provides germline-transmission testing for mouse ESC/iPSC lines via blastocyst injection. Chimeric mice are generated and bred to determine germline transmission.

We proide full report of genotyping results.

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Mouse
Embryonic stem cell generation
Price on request

Mouse Embryonic Stem Cell Derivation

Our scientists have successfully derived over a hundred new mouse embryonic stem cell lines, including those from gene-targeted embryos.

Turnaround time: 2 months

Deliverable: 2 vials of frozen ESCs (1x10^6 cells/vial)

Mouse Embryonic Stem Cell Derivation

Our scientists have successfully derived over a hundred new mouse embryonic stem cell lines, including those from gene-targeted embryos.

Turnaround time: 2 months

Deliverable: 2 vials of frozen ESCs (1x10^6 cells/vial)

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Mouse
FBS Testing
Price on request

Are you looking for the best FBS for your stem cell growth, or screening assays?

Does your facility need a tested FBS for your research programs?

When consistency is important, consider our FBS evaluation service.
Simply provide us your FBS candidates (samples) to find out which batches/brands are qualified for your... Show more »

Are you looking for the best FBS for your stem cell growth, or screening assays?

Does your facility need a tested FBS for your research programs?

When consistency is important, consider our FBS evaluation service.
Simply provide us your FBS candidates (samples) to find out which batches/brands are qualified for your research. We will provide you with a full service report containing high resolution staining images.

FBS test for ES cell growth support:
Timeline: 2-3 months
Mouse ESCs will be cultured using customer’s FBS containing medium for up to 10 passages
ES cell colony formation and morphology observation
ES cell biomarker staining

FBS test for mouse ES cell germline transmission:
Timeline: 5-6 months
ES cells will be cultured with customer’s FBS-containing medium for up to 5 passages and microinjected into mouse blastocytes to test for germline transmission.

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Pluripotency analysis
Price on request

Human ES/iPS Cell Pluripotency Characterization Service

ASC provides services on identifying the pluripotent status of human embryonic stem cells (ESC) or induced pluripotent stem cells (iPS) with a set of commonly-used ESC antibody markers and alkaline phosphatase (AP) activity.

We provide a full report of results,... Show more »

Human ES/iPS Cell Pluripotency Characterization Service

ASC provides services on identifying the pluripotent status of human embryonic stem cells (ESC) or induced pluripotent stem cells (iPS) with a set of commonly-used ESC antibody markers and alkaline phosphatase (AP) activity.

We provide a full report of results, containing high-definition, publication-grade images.


Mouse ES/iPS Cell Pluripotency Characterization Service

ASC provides services for identifying the pluripotent status of mouse embryonic stem cells (ESC) or induced pluripotent stem cells (iPSC) with a set of commonly-used ESC antibody markers and alkaline phosphatase (AP) activity.

We provide a full report of results, containing high-definition, publication-grade images.

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Human
Mouse
Toxicology
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Tissue Fixation
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Biospecimens
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Plasmids and Vectors
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Biochemistry & Molecular Biology
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Genetic Engineering
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Cell and Tissue Culture
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Cells and Tissues
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Nucleic Acid Services
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Biology
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Mammalian Cell Culture
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Transgenic Mouse Services
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Embryo Transfer
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Embryonic stem cell gene targeting
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BAC Transgenic Mouse Production
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Embryonic stem cell electroporation
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Embryonic stem cell screening
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Embryo Isolation
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Pronuclear injection
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Cell Culture Media and Reagents
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Induced Pluripotent Stem Cell (iPSC) Generation
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Embryonic Stem Cell (ESC) Culture
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Pharmacology & Toxicology
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Tissue Processing
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Transgenic Animal Model Development
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In vitro Toxicity Testing
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DNA
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June 22, 2017

Positive review received for CRISPR/Cas9 vector generation:

"Very fast and reliable service."

2016-12-09 10:39:29 -0800

Net Promoter Score of 10 received for CRISPR Modified Cell Lines.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 10.
November 17, 2016

Positive review received for CRISPR Modified Cell Lines:

"We contacted Applied StemCell for generating a CRISPR KO line attending at their expertise working with the Jurkat cell line. They were very professional and efficient generating the cell line in a very short time and the price was very competitive compared with other companies in the market. I recommend their services for other customers."

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